##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364639_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1363962 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.50014516533452 31.0 27.0 31.0 12.0 34.0 2 27.23981313262393 31.0 27.0 31.0 12.0 34.0 3 27.792957575064406 31.0 27.0 31.0 16.0 34.0 4 31.191642435786335 35.0 32.0 37.0 16.0 37.0 5 31.565132313070304 35.0 32.0 37.0 17.0 37.0 6 31.858282708755816 35.0 32.0 37.0 18.0 37.0 7 31.79990864848141 35.0 32.0 37.0 17.0 37.0 8 31.497304910254098 35.0 32.0 37.0 16.0 37.0 9 32.53815208928108 37.0 32.0 39.0 11.0 39.0 10-11 32.5174121419805 37.0 32.0 39.0 13.0 39.0 12-13 32.508074271863876 37.0 32.0 39.0 11.0 39.0 14-15 33.26587031016993 38.0 32.0 40.0 11.5 41.0 16-17 33.69950152570233 38.0 32.0 41.0 14.5 41.0 18-19 33.54365920751458 38.0 32.0 41.0 12.0 41.0 20-21 33.446354077313 38.0 32.0 41.0 12.5 41.0 22-23 33.51932348555165 38.0 32.0 41.0 14.0 41.0 24-25 33.5851251721089 38.0 32.0 41.0 16.0 41.0 26-27 33.3492945551269 38.0 32.0 40.5 12.5 41.0 28-29 33.22240832222599 38.0 32.0 40.0 10.5 41.0 30-31 33.063063340474294 38.0 31.0 40.0 10.0 41.0 32-33 32.923520230035734 38.0 31.0 40.0 9.0 41.0 34-35 32.95294370371022 38.0 31.0 40.0 8.5 41.0 36-37 32.87825723883803 38.0 31.0 40.0 8.0 41.0 38-39 32.568099771107995 38.0 30.0 40.0 4.5 41.0 40-41 32.41775577325468 38.0 30.0 40.0 2.0 41.0 42-43 31.90768511146205 37.5 30.0 40.0 2.0 41.0 44-45 31.80455650523988 37.0 30.0 40.0 2.0 41.0 46-47 31.769645708604784 37.0 30.0 40.0 2.0 41.0 48-49 31.689970834964612 37.0 30.0 40.0 2.0 41.0 50-51 31.437998272679152 37.0 30.0 40.0 2.0 41.0 52-53 31.331896709732383 37.0 29.5 40.0 2.0 41.0 54-55 31.134189955438643 36.5 29.5 40.0 2.0 41.0 56-57 30.887104992661087 36.0 29.0 40.0 2.0 41.0 58-59 30.655387026911306 36.0 29.0 40.0 2.0 41.0 60-61 30.332649296681286 35.0 28.0 39.0 2.0 41.0 62-63 30.137630300550896 35.0 28.0 39.0 2.0 40.5 64-65 29.87655814458174 35.0 28.0 39.0 2.0 40.0 66-67 29.573981166630745 35.0 28.0 38.5 2.0 40.0 68-69 29.30974103384112 34.0 28.0 38.0 2.0 40.0 70-71 28.828697940265197 34.0 27.0 37.0 2.0 40.0 72-73 28.581736148074505 34.0 27.0 37.0 2.0 39.0 74-75 28.528625064334637 34.0 27.5 36.0 2.0 39.0 76-77 28.255505651916987 34.0 27.0 36.0 2.0 39.0 78-79 27.942308143482002 34.0 26.0 35.5 2.0 37.5 80-81 27.522893599675065 34.0 26.0 35.0 2.0 37.0 82-83 27.650952885784207 34.0 26.5 35.0 2.0 37.0 84-85 27.49230916990356 34.0 26.5 35.0 2.0 36.0 86-87 27.38650636894576 34.0 27.0 35.0 2.0 36.0 88-89 27.21948265420884 34.0 26.0 35.0 2.0 36.0 90-91 27.08809702909612 34.0 26.0 35.0 2.0 35.0 92-93 26.97490692555951 34.0 26.0 35.0 2.0 35.0 94-95 26.860347648981424 34.0 26.0 35.0 2.0 35.0 96-97 26.745455518555502 34.0 25.0 35.0 2.0 35.0 98-99 26.650104988262136 34.0 25.0 35.0 2.0 35.0 100-101 26.53321096922055 33.0 25.0 35.0 2.0 35.0 102-103 25.352501389334893 31.5 23.5 34.0 2.0 35.0 104-105 26.214552531522138 33.0 24.5 35.0 2.0 35.0 106-107 26.361456550842327 33.0 25.0 35.0 2.0 35.0 108-109 26.30046548217619 33.0 25.0 35.0 2.0 35.0 110-111 26.211603035861703 33.0 24.0 35.0 2.0 35.0 112-113 26.09053551345272 33.0 24.0 35.0 2.0 35.0 114-115 25.960505864532884 33.0 24.0 35.0 2.0 35.0 116-117 25.82288876083058 33.0 23.0 35.0 2.0 35.0 118-119 25.647505942247655 33.0 21.5 35.0 2.0 35.0 120-121 25.409974398113732 33.0 19.0 35.0 2.0 35.0 122-123 25.154688693673286 33.0 10.0 35.0 2.0 35.0 124-125 24.10508540560514 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.6503988571569828 1101 2 2.0292217128904824 1101 3 1.5480564319213954 1101 4 1.7487964618918852 1101 5 1.4552939547686314 1101 6 1.614918203619041 1101 7 1.43924245797675 1101 8 1.7701881630977674 1101 9 2.182237512729351 1101 10-11 2.1602859934141136 1101 12-13 2.2241959685184796 1101 14-15 2.411467692324493 1101 16-17 1.994526444109134 1101 18-19 2.2289094037535264 1101 20-21 2.3075224431845314 1101 22-23 2.153845871761739 1101 24-25 2.024398203883983 1101 26-27 2.382654769684713 1101 28-29 2.3928870091793044 1101 30-31 2.5349092749204374 1101 32-33 2.4114303601268006 1101 34-35 2.150947485630347 1101 36-37 1.9710943659210898 1101 38-39 2.0712165472757746 1101 40-41 2.1415063680648743 1101 42-43 2.3823554519984143 1101 44-45 2.2910352065729036 1101 46-47 1.9430522157781454 1101 48-49 1.6239156410659774 1101 50-51 1.8093571566224185 1101 52-53 1.8405571418952675 1101 54-55 1.727302898467567 1101 56-57 1.8764076448348739 1101 58-59 1.6760442285621373 1101 60-61 1.7969476652610616 1101 62-63 1.7422684707329452 1101 64-65 1.571661941472538 1101 66-67 1.5518623277378758 1101 68-69 1.4105010024019542 1101 70-71 1.3193766218173302 1101 72-73 1.1089528398042745 1101 74-75 1.1171715310119374 1101 76-77 1.2837793191013134 1101 78-79 1.2199414195013425 1101 80-81 1.3614076270047022 1101 82-83 1.3391074443707005 1101 84-85 1.4146120825927149 1101 86-87 1.2993254259892844 1101 88-89 1.3004198930107869 1101 90-91 1.3151649584361458 1101 92-93 1.3705485356412765 1101 94-95 1.3142145400845955 1101 96-97 1.25693248787017 1101 98-99 1.2808061131067952 1101 100-101 1.2137449363395731 1101 102-103 1.1370888915979087 1101 104-105 1.2840197473252566 1101 106-107 1.3522394004101557 1101 108-109 1.2500574823631645 1101 110-111 1.240592635758997 1101 112-113 1.0998594360533502 1101 114-115 1.1743107520117526 1101 116-117 1.1579252468029146 1101 118-119 1.0994007683611322 1101 120-121 1.0761436585144857 1101 122-123 1.2265658566358901 1101 124-125 1.1400001813012395 1102 1 1.1248140205325186 1102 2 1.5112723175410778 1102 3 1.0633812715898543 1102 4 1.012123742865434 1102 5 0.768124601190177 1102 6 0.8074099687956249 1102 7 0.8687935500230104 1102 8 0.7786278708537928 1102 9 1.4135824753487753 1102 10-11 1.398332201068591 1102 12-13 1.384455227381828 1102 14-15 1.648628825077843 1102 16-17 1.3700424090532906 1102 18-19 1.2351153631645388 1102 20-21 1.5953687804379015 1102 22-23 1.4914807581947258 1102 24-25 1.288521734125169 1102 26-27 1.6042929230685203 1102 28-29 1.3075127223931418 1102 30-31 1.3051895159764086 1102 32-33 1.4502457621353244 1102 34-35 1.4769597033544528 1102 36-37 1.3026768925700551 1102 38-39 1.7057955264987186 1102 40-41 1.6890665137083687 1102 42-43 1.9605934008541865 1102 44-45 1.57827503527432 1102 46-47 1.3784632544883024 1102 48-49 1.345231706085329 1102 50-51 1.4417226214197285 1102 52-53 1.4475252201514195 1102 54-55 1.2988476195016325 1102 56-57 1.1914211366596028 1102 58-59 1.3224429981362285 1102 60-61 1.174715067322527 1102 62-63 1.0524347299215648 1102 64-65 0.9824872410490784 1102 66-67 1.1507891410151956 1102 68-69 1.0170243162038304 1102 70-71 1.2525945072785873 1102 72-73 1.0056557544558231 1102 74-75 1.1954017171690872 1102 76-77 0.9497187099865307 1102 78-79 1.0223241098541642 1102 80-81 1.0618081822539942 1102 82-83 1.1608060195464525 1102 84-85 1.0086875909985729 1102 86-87 1.1193182435841926 1102 88-89 0.9736592747843815 1102 90-91 1.1335190830760702 1102 92-93 1.1750966371397062 1102 94-95 1.0562299046285588 1102 96-97 0.8970787218414671 1102 98-99 1.0050162648840946 1102 100-101 1.1614051317152487 1102 102-103 0.9929088540009943 1102 104-105 1.0887639524070991 1102 106-107 1.1099022020164497 1102 108-109 1.151416489104239 1102 110-111 1.0454205761801596 1102 112-113 0.8900642338592313 1102 114-115 1.1181723153530818 1102 116-117 1.2117963391749562 1102 118-119 1.2113854587923463 1102 120-121 1.15276340284937 1102 122-123 1.0559483581387994 1102 124-125 1.0749208974241853 1103 1 1.0682178669970277 1103 2 1.4519698998271267 1103 3 1.06866328180514 1103 4 0.9144105284036144 1103 5 1.1132381583839184 1103 6 1.1079583757691367 1103 7 1.0759355847880165 1103 8 1.1900336179086146 1103 9 1.618332874147086 1103 10-11 1.6066596687781711 1103 12-13 1.654377257536396 1103 14-15 1.9364111987694912 1103 16-17 1.473026607639845 1103 18-19 1.5504710404857391 1103 20-21 1.94163853861523 1103 22-23 1.6816782559901498 1103 24-25 1.6827960135124584 1103 26-27 1.9280053513444386 1103 28-29 1.739558157066 1103 30-31 1.9604964887478644 1103 32-33 1.9245206107765895 1103 34-35 1.6170278497224686 1103 36-37 1.5181355484485906 1103 38-39 1.6579095998673523 1103 40-41 1.8524926636462737 1103 42-43 2.0386909438052996 1103 44-45 1.83979544084983 1103 46-47 1.672990262127847 1103 48-49 1.511767213558585 1103 50-51 1.5657137020817373 1103 52-53 1.127633588672083 1103 54-55 1.2303403393330292 1103 56-57 1.2698735658740254 1103 58-59 1.2550319165049508 1103 60-61 1.2825024104475418 1103 62-63 1.2513738661581435 1103 64-65 1.1497817538253692 1103 66-67 1.112248210756757 1103 68-69 1.1366056016972657 1103 70-71 1.1897445090350693 1103 72-73 1.123267344261663 1103 74-75 1.111231234321199 1103 76-77 1.0506660306593503 1103 78-79 1.1554894106959175 1103 80-81 1.0898058621594053 1103 82-83 1.044494482742774 1103 84-85 1.0442625028988282 1103 86-87 1.0121990046203457 1103 88-89 1.1459470680559534 1103 90-91 1.1540582537755952 1103 92-93 1.1912563985248497 1103 94-95 1.1054752456565637 1103 96-97 1.1640188355537973 1103 98-99 1.1811953706476395 1103 100-101 1.1869992520909243 1103 102-103 1.0692204267035734 1103 104-105 1.1700818830611972 1103 106-107 1.2237458176920626 1103 108-109 1.3090970201823033 1103 110-111 1.3302082123940444 1103 112-113 1.2901228516336474 1103 114-115 1.2808866413986806 1103 116-117 1.2191243302392074 1103 118-119 1.1176340725178804 1103 120-121 1.1861032236266773 1103 122-123 0.9463320942962454 1103 124-125 0.8717431268432811 1104 1 1.2537025392515453 1104 2 1.6334478058182604 1104 3 1.1296065106964477 1104 4 1.2069348868291243 1104 5 0.9049366057987918 1104 6 0.8932634648186095 1104 7 0.8284959334542101 1104 8 1.0401584306894414 1104 9 1.308020210464477 1104 10-11 1.4430551983339583 1104 12-13 1.5324153600877324 1104 14-15 1.5291629801882678 1104 16-17 1.4092878177902222 1104 18-19 1.404699893766015 1104 20-21 1.7410593091792848 1104 22-23 1.515427509260121 1104 24-25 1.4076544261392172 1104 26-27 1.597684002900273 1104 28-29 1.5937715544146869 1104 30-31 1.661095344763595 1104 32-33 1.6460827393282358 1104 34-35 1.5613487148621132 1104 36-37 1.4197495893037697 1104 38-39 1.491610154686228 1104 40-41 1.539875103500009 1104 42-43 1.8175764239389096 1104 44-45 1.6397056492473077 1104 46-47 1.5135151014045576 1104 48-49 1.2413199360718608 1104 50-51 1.2375734799824514 1104 52-53 1.0413355173515413 1104 54-55 1.0398851130814997 1104 56-57 1.201111720926459 1104 58-59 1.1792511159612964 1104 60-61 1.1895889550777703 1104 62-63 1.032619074512688 1104 64-65 0.9473521059139429 1104 66-67 0.94115963477552 1104 68-69 0.9240607825623322 1104 70-71 0.8971523162431758 1104 72-73 0.8508881979933527 1104 74-75 0.79926073797726 1104 76-77 0.8460804029583926 1104 78-79 0.7709047397907263 1104 80-81 0.9543580510750473 1104 82-83 1.03274760674228 1104 84-85 0.969117358207999 1104 86-87 1.0011253203065245 1104 88-89 1.0352259261815426 1104 90-91 1.0187798446080194 1104 92-93 0.8365952698247803 1104 94-95 0.7732901081294372 1104 96-97 0.6893912192387148 1104 98-99 0.7444865518120132 1104 100-101 0.7901812113387834 1104 102-103 0.8734194107827378 1104 104-105 0.8347223915858777 1104 106-107 0.9042733065570445 1104 108-109 0.8508320834350904 1104 110-111 0.8676196627860229 1104 112-113 0.9558411047208466 1104 114-115 0.9626149089482148 1104 116-117 0.9011229539252987 1104 118-119 0.9219769439601038 1104 120-121 1.054874759198011 1104 122-123 1.0390880463632328 1104 124-125 0.9278436198384341 1105 1 1.1066467427012867 1105 2 1.3504827468300924 1105 3 0.8529591538372472 1105 4 0.9438286063901487 1105 5 0.7637236722892773 1105 6 0.8634815666688738 1105 7 0.8986074508454571 1105 8 0.9885455274636321 1105 9 1.193682175664236 1105 10-11 0.932020808580269 1105 12-13 1.1614601575003647 1105 14-15 1.3637627088178448 1105 16-17 1.1108091009677423 1105 18-19 1.080612515802862 1105 20-21 1.0760688142417436 1105 22-23 1.1679159192352984 1105 24-25 0.8958802148464713 1105 26-27 1.3347203871200932 1105 28-29 1.465891141941114 1105 30-31 1.3140740905685249 1105 32-33 1.4617443851295064 1105 34-35 1.251285178221778 1105 36-37 0.9465337551827773 1105 38-39 1.2409766218668494 1105 40-41 1.2446285810989366 1105 42-43 1.4585628211121175 1105 44-45 1.2756175141914277 1105 46-47 0.8532628239158768 1105 48-49 0.8357071582395292 1105 50-51 0.9246051915206621 1105 52-53 0.7414451774997204 1105 54-55 0.9076445563344429 1105 56-57 0.9755210407377994 1105 58-59 1.0807295760820317 1105 60-61 0.9574868043429774 1105 62-63 0.9050016334624118 1105 64-65 0.6287646516500303 1105 66-67 0.5927154011603655 1105 68-69 0.49702441017208443 1105 70-71 0.829392163215811 1105 72-73 0.653274932782594 1105 74-75 0.5588022946781805 1105 76-77 0.7465984199906117 1105 78-79 0.7770348248808467 1105 80-81 0.9434998036737099 1105 82-83 0.7570010719201541 1105 84-85 0.6344901849674436 1105 86-87 0.8227643314014799 1105 88-89 0.8245776537133374 1105 90-91 0.584643598675914 1105 92-93 0.7739414705797429 1105 94-95 0.7266421952802773 1105 96-97 0.774000588970857 1105 98-99 0.8489556464592489 1105 100-101 0.8338933788834026 1105 102-103 0.6646604395297864 1105 104-105 0.6542118508442343 1105 106-107 0.8099824954150066 1105 108-109 0.781381011143381 1105 110-111 0.8655324528028103 1105 112-113 0.7644474744730978 1105 114-115 0.8405499149044076 1105 116-117 0.8874285662299499 1105 118-119 0.9208657921026848 1105 120-121 0.8573283310786444 1105 122-123 0.7824857838389683 1105 124-125 0.7714658826229481 1106 1 0.3804853056827504 1106 2 -1.6355874847990606 1106 3 -0.058657943388318756 1106 4 0.33060800952568314 1106 5 0.38804567367348497 1106 6 0.5132976325090297 1106 7 0.5346561952785329 1106 8 0.5304904619394399 1106 9 1.0899533243311836 1106 10-11 1.0840296733416395 1106 12-13 1.1388413572852087 1106 14-15 1.257208079620085 1106 16-17 0.8385832531403494 1106 18-19 0.8667629600283036 1106 20-21 1.1438168385481404 1106 22-23 0.8569725655024456 1106 24-25 0.9457645977454803 1106 26-27 0.9189465518483715 1106 28-29 1.0541347540845294 1106 30-31 1.0405721759982356 1106 32-33 1.085776285857719 1106 34-35 1.002940679696259 1106 36-37 0.7894796309576364 1106 38-39 1.063261289130331 1106 40-41 1.011764619969604 1106 42-43 1.2071699148019306 1106 44-45 1.1116731467519152 1106 46-47 0.901483947381216 1106 48-49 0.7124247233875849 1106 50-51 0.718798406080424 1106 52-53 0.5441566312566408 1106 54-55 0.531908373024681 1106 56-57 0.5247355356147274 1106 58-59 0.4878485850568133 1106 60-61 0.5756916549011741 1106 62-63 0.4161082099549489 1106 64-65 0.4396072694581683 1106 66-67 0.3727090819499672 1106 68-69 0.2724272598350588 1106 70-71 0.3873972486756081 1106 72-73 0.2975056206361657 1106 74-75 0.4090645136990041 1106 76-77 0.1462558003017591 1106 78-79 0.2136888546939808 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-1.8850334129137387 2216 84-85 -1.8159573756167333 2216 86-87 -2.0172264405452403 2216 88-89 -2.083350548019933 2216 90-91 -1.9646364125582387 2216 92-93 -1.9356830880785765 2216 94-95 -2.1190066772923473 2216 96-97 -2.094122806150313 2216 98-99 -2.1947144105139493 2216 100-101 -2.3006480269904515 2216 102-103 -2.01854209617877 2216 104-105 -1.9958524446139592 2216 106-107 -1.9475008539671137 2216 108-109 -1.9443138010857943 2216 110-111 -1.8794073642410005 2216 112-113 -2.193106991102251 2216 114-115 -2.056081344123033 2216 116-117 -1.8729423613900131 2216 118-119 -1.927436446703286 2216 120-121 -2.1031652694670164 2216 122-123 -1.9562190789438958 2216 124-125 -1.7704078134130015 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 75933.0 3 11924.0 4 8132.0 5 9067.0 6 10424.0 7 9696.0 8 8960.0 9 9064.0 10 10195.0 11 10885.0 12 11463.0 13 11748.0 14 12007.0 15 10456.0 16 8191.0 17 7263.0 18 6518.0 19 6264.0 20 6469.0 21 6854.0 22 8327.0 23 11568.0 24 16684.0 25 20697.0 26 23239.0 27 25203.0 28 29209.0 29 33926.0 30 39925.0 31 46958.0 32 57571.0 33 73935.0 34 97926.0 35 137031.0 36 202271.0 37 230277.0 38 57660.0 39 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.219216944867036 20.15758361988351 14.35867977072068 23.264519664528773 2 14.005701045632616 22.865396387280157 39.24410807442371 23.884794492663524 3 18.707568019833573 26.19508013275319 29.72447978778312 25.37287205963012 4 12.679426540793026 19.458710025538373 36.050638606313576 31.811224827355023 5 13.793917413422635 38.68056198993736 33.15169992690542 14.37382066973458 6 30.500813024856594 37.64602668543763 17.28932442888035 14.563835860825428 7 26.228274860847435 34.66823794941723 21.13341013418598 17.970077055549353 8 23.934390104159014 35.827909914939255 20.936990406793107 19.300709574108623 9 24.27580624309522 18.834032625813883 20.83737336342054 36.05278776767036 10-11 22.799719848882845 28.871018895517437 27.80022641143745 20.52903484416227 12-13 23.7934930150127 26.9174156373101 27.22803085738245 22.061060490294743 14-15 21.57057011623244 28.358115780026473 25.394944997804025 24.676369105937066 16-17 21.415223803226738 30.739140618517947 26.049335760276023 21.796299817979296 18-19 21.467097532314924 29.58266010575793 27.7069991186839 21.243243243243242 20-21 22.386147532044447 28.791658144615024 27.401153074896996 21.42104124844354 22-23 22.31113259292097 28.47930945448976 27.156843696939493 22.05271425564978 24-25 22.299000764969886 28.41391224256633 27.39943657378691 21.88765041867687 26-27 21.912638843111555 28.43575269143162 27.490467597547973 22.161140867908845 28-29 21.91882422833375 28.6880737515614 27.470275621052448 21.922826399052404 30-31 22.21645870273088 28.3461268781915 27.17080942574054 22.266604993337076 32-33 22.03174216113607 28.349399541609028 27.03559268939476 22.583265607860145 34-35 22.656590137022107 28.311220717701275 26.848932082173615 22.183257063103 36-37 22.757305988287513 27.93556479734048 26.92411184460488 22.383017369767128 38-39 22.179935649661537 28.140934732364432 27.108553189645484 22.570576428328543 40-41 22.620100936978112 27.965698616331135 27.225180694526625 22.18901975216413 42-43 22.38915579883138 28.02602178970192 26.907242148271564 22.677580263195136 44-45 22.37097186362715 28.158843174748643 26.931860572361792 22.538324389262414 46-47 22.654054220742033 28.23835452294734 26.44659864844624 22.660992607864387 48-49 22.408446428040417 28.20264496051062 26.523726851639395 22.865181759809566 50-51 22.434838860321626 28.257432852654357 26.530535071404287 22.77719321561973 52-53 22.62080017273561 28.150799948737475 26.247219760894847 22.981180117632068 54-55 22.545750412508234 28.190779642397402 26.249696172173266 23.013773772921102 56-57 22.432140390607874 28.337410655966128 26.1948427835061 23.035606169919898 58-59 22.56187001240704 27.93775190326914 26.352183712274158 23.14819437204966 60-61 22.423179335286164 28.092751997221637 26.491780745979398 22.992287921512798 62-63 22.45627623471802 27.967572190785933 26.59741098779277 22.978740586703278 64-65 22.386386536299376 28.22505426158544 26.17983388778708 23.208725314328106 66-67 22.350129356871182 28.189778219672153 26.089128333981126 23.37096408947554 68-69 22.575507882035563 27.921344809234554 26.300233066010904 23.20291424271898 70-71 22.655310621242485 27.831707370785523 26.197743472292572 23.315238535679416 72-73 22.71970837044205 27.86472868345063 26.203102196946094 23.212460749161224 74-75 22.514043686249295 28.214488592578242 26.10048629979142 23.170981421381047 76-77 22.67520496353655 27.881867888856306 26.28613082210409 23.156796325503056 78-79 22.778467027360353 28.064513760929096 26.193996432215773 22.963022779494786 80-81 22.946492812357754 27.812284334023463 26.24682466264335 22.994398190975435 82-83 22.853759273324506 27.795176127547055 26.36206685787516 22.988997741253275 84-85 22.798248869205125 27.790483888077095 26.290416534069198 23.120850708648586 86-87 22.786404648046332 27.681539068738587 26.35089812664641 23.18115815656867 88-89 22.913450492921267 27.571467434301944 26.205759981320647 23.309322091456142 90-91 22.698685214694674 27.737245569386065 26.234403053259136 23.329666162660125 92-93 22.814737971188777 27.57409934192244 26.34516419268123 23.265998494207548 94-95 22.682958261691365 27.356199283818956 26.496569079380954 23.464273375108725 96-97 22.626302526931617 27.387023777357474 26.5022379380426 23.484435757668315 98-99 22.49817551206805 27.612248224336394 26.2993878237273 23.590188439868253 100-101 22.315710348467572 27.593796946187766 26.493973820796178 23.596518884548484 102-103 22.176868899974078 27.471017648263995 26.573807605797423 23.7783058459645 104-105 22.17326581141183 27.68440377208636 26.481274998660375 23.661055417841435 106-107 22.131223056439016 27.8756590791886 26.284472617358766 23.708645247013614 108-109 21.99048378381553 28.050592616085794 26.146358259623405 23.812565340475267 110-111 21.922891445994576 28.202916783801417 26.32386737728009 23.550324392923912 112-113 22.090497153065133 28.2061594782601 26.27477214979912 23.42857121887564 114-115 21.952668562879417 28.315341819197297 26.101609456746424 23.630380161176863 116-117 21.85903203977412 28.281482340472692 26.25402387093081 23.60546174882238 118-119 22.034339229968783 28.174584537439696 26.16761013812305 23.623466094468473 120-121 21.99232603139591 28.342061757175905 26.118643458378372 23.546968753049814 122-123 22.113883496214527 28.212148013314298 26.06211626891361 23.611852221557566 124-125 22.26518706465069 28.094688853112714 25.932130554241574 23.707993527995015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5088.0 1 3884.5 2 2396.5 3 1860.0 4 1442.5 5 1179.0 6 1053.0 7 1091.0 8 1222.0 9 1223.0 10 1103.0 11 1031.0 12 1052.0 13 1076.0 14 1110.0 15 1191.5 16 1267.5 17 1374.0 18 1580.0 19 1761.0 20 1961.0 21 2275.0 22 2780.5 23 3456.0 24 4066.0 25 4973.5 26 6373.0 27 8276.5 28 10094.5 29 11971.5 30 14500.0 31 17680.0 32 21309.0 33 23921.5 34 25875.5 35 28286.5 36 31551.5 37 34897.0 38 37143.5 39 38794.5 40 39999.5 41 41516.5 42 43202.0 43 45216.0 44 47638.5 45 49476.0 46 49935.5 47 50287.0 48 51262.5 49 51107.0 50 49423.0 51 47621.0 52 46630.0 53 46200.0 54 45187.5 55 42140.5 56 38478.0 57 35369.5 58 32905.0 59 29763.0 60 25379.5 61 20973.0 62 17090.0 63 13083.5 64 9585.0 65 7517.0 66 5836.5 67 4697.5 68 3830.0 69 3019.5 70 2285.5 71 1594.5 72 1251.0 73 955.0 74 700.0 75 577.0 76 431.5 77 302.0 78 233.5 79 149.5 80 116.5 81 106.5 82 79.0 83 44.0 84 30.5 85 25.5 86 18.5 87 17.0 88 14.0 89 13.0 90 7.5 91 3.0 92 3.0 93 2.0 94 1.0 95 0.5 96 0.5 97 0.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19450688508917405 2 0.23160469279935952 3 0.28182603327658684 4 0.2680426580799172 5 0.2991285680979382 6 0.21958089741503062 7 0.21012315592369876 8 0.1893014614776658 9 0.18959472477972258 10-11 0.134901118946129 12-13 0.15751905111725986 14-15 0.1744916647237973 16-17 0.1487578099683129 18-19 0.1731719798645417 20-21 0.16851642494438995 22-23 0.19087775172622112 24-25 0.18112674693283243 26-27 0.23523382616231245 28-29 0.1613681319567554 30-31 0.14274591227614847 32-33 0.18039358867769043 34-35 0.14069306916175084 36-37 0.14014320047039433 38-39 0.17251213743491386 40-41 0.1919041732834199 42-43 0.26690626278444707 44-45 0.15880207806375837 46-47 0.14472543956503187 48-49 0.1476214146728428 50-51 0.1516904429888809 52-53 0.17181563709252898 54-55 0.1591686571913294 56-57 0.15847215684894447 58-59 0.1343145923420154 60-61 0.1471815197197576 62-63 0.1363307775436559 64-65 0.13273830209346008 66-67 0.12064119088361698 68-69 0.12368379764245631 70-71 0.12405037677002731 72-73 0.10348528771329407 74-75 0.10469499883427838 76-77 0.12518677206549744 78-79 0.12991564281116336 80-81 0.13944670012800942 82-83 0.14102299037656474 84-85 0.12877924751569325 86-87 0.15392657566706403 88-89 0.1488677837065842 90-91 0.13757714657739734 92-93 0.13845693648356772 94-95 0.15803226189585926 96-97 0.14619175607531587 98-99 0.14193943819549226 100-101 0.12445361381035543 102-103 0.16019507874852817 104-105 0.12016463801777469 106-107 0.12753287848195186 108-109 0.13680733040949822 110-111 0.11517916188280905 112-113 0.10586805204250559 114-115 0.10421844596843607 116-117 0.1076276318548464 118-119 0.09267120344994949 120-121 0.08599946332815724 122-123 0.11122010730504223 124-125 0.1083974480227455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1363962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.01287148539721 #Duplication Level Percentage of deduplicated Percentage of total 1 83.47922658350647 55.94182682740875 2 9.747545186282887 13.064219857329547 3 2.8644811642473367 5.758713243961434 4 1.258571739935557 3.37362025053817 5 0.7093271772143328 2.3767025483881836 6 0.4255133711125395 1.7108923712209647 7 0.28045593668400637 1.3155910349625413 8 0.20069891981336122 1.0759528736568655 9 0.15813268456207782 0.9537232741379442 >10 0.8177879959051998 9.645616047363731 >50 0.04084781274982542 1.8714917836482117 >100 0.01616777080798231 1.9079964880619769 >500 9.044779479427413E-4 0.41973577657152444 >1k 2.2611948698568533E-4 0.18706274952258012 >5k 1.1305974349284267E-4 0.3968548732277337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5238 0.38402829404338246 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1364 0.10000278600136954 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 7.331582551420055E-5 0.0 7 0.0 0.0 0.0 7.331582551420055E-5 0.0 8 0.0 0.0 0.0 7.331582551420055E-5 0.0 9 0.0 0.0 0.0 7.331582551420055E-5 0.0 10-11 0.0 0.0 0.0 7.331582551420055E-5 0.0 12-13 0.0 0.0 0.0 7.331582551420055E-5 7.331582551420055E-5 14-15 0.0 0.0 0.0 7.331582551420055E-5 1.8328956378550136E-4 16-17 0.0 0.0 0.0 7.331582551420055E-5 2.1994747654260162E-4 18-19 0.0 0.0 0.0 7.331582551420055E-5 2.1994747654260162E-4 20-21 0.0 0.0 0.0 1.466316510284011E-4 2.1994747654260162E-4 22-23 0.0 0.0 0.0 6.231845168707046E-4 2.1994747654260162E-4 24-25 0.0 0.0 0.0 0.001759579812340813 2.1994747654260162E-4 26-27 0.0 0.0 0.0 0.003262554235381924 2.932633020568022E-4 28-29 0.0 0.0 0.0 0.007844793330019457 2.932633020568022E-4 30-31 0.0 0.0 0.0 0.01876885133163534 2.932633020568022E-4 32-33 0.0 0.0 0.0 0.043073047489592814 2.932633020568022E-4 34-35 0.0 0.0 0.0 0.0814538821462768 2.932633020568022E-4 36-37 7.331582551420055E-5 0.0 0.0 0.13926341056422392 2.932633020568022E-4 38-39 7.331582551420055E-5 0.0 0.0 0.22101055601255754 2.932633020568022E-4 40-41 7.331582551420055E-5 0.0 0.0 0.3070466772534719 2.932633020568022E-4 42-43 1.466316510284011E-4 0.0 0.0 0.3910666132927457 2.932633020568022E-4 44-45 2.1994747654260162E-4 0.0 0.0 0.49957403505376247 2.932633020568022E-4 46-47 2.566053892997019E-4 0.0 0.0 0.6288298354352981 2.932633020568022E-4 48-49 2.932633020568022E-4 0.0 0.0 0.7503874741378425 2.932633020568022E-4 50-51 2.932633020568022E-4 0.0 0.0 0.8732281397868855 2.932633020568022E-4 52-53 2.932633020568022E-4 0.0 0.0 0.9953356471807865 2.932633020568022E-4 54-55 3.665791275710027E-4 0.0 0.0 1.1354788476511808 2.932633020568022E-4 56-57 3.665791275710027E-4 0.0 0.0 1.3009893237494887 2.932633020568022E-4 58-59 3.665791275710027E-4 0.0 0.0 1.4802098592189519 2.932633020568022E-4 60-61 3.665791275710027E-4 0.0 0.0 1.6650390553402514 2.932633020568022E-4 62-63 3.665791275710027E-4 0.0 0.0 1.8534240689989896 2.932633020568022E-4 64-65 3.665791275710027E-4 0.0 0.0 2.069155885574525 2.932633020568022E-4 66-67 4.3989495308520323E-4 0.0 0.0 2.310621556905544 2.932633020568022E-4 68-69 5.132107785994038E-4 0.0 0.0 2.588781798906421 2.932633020568022E-4 70-71 5.498686913565041E-4 0.0 0.0 2.886847287534404 2.932633020568022E-4 72-73 5.865266041136044E-4 0.0 0.0 3.1952869654726452 3.2992121481390245E-4 74-75 5.865266041136044E-4 0.0 0.0 3.5333095790058664 4.3989495308520323E-4 76-77 5.865266041136044E-4 0.0 0.0 3.9037011295036077 5.132107785994038E-4 78-79 5.865266041136044E-4 0.0 0.0 4.3334418407550945 5.132107785994038E-4 80-81 5.865266041136044E-4 0.0 0.0 4.800793570495365 5.132107785994038E-4 82-83 5.865266041136044E-4 0.0 0.0 5.290763232406768 5.132107785994038E-4 84-85 6.231845168707046E-4 0.0 0.0 5.80778643393291 5.132107785994038E-4 86-87 6.598424296278049E-4 0.0 0.0 6.359158099712455 5.132107785994038E-4 88-89 6.598424296278049E-4 0.0 0.0 6.952319786035094 5.132107785994038E-4 90-91 7.331582551420054E-4 0.0 0.0 7.608349792736162 5.498686913565041E-4 92-93 8.06474080656206E-4 0.0 0.0 8.290443575407526 6.598424296278049E-4 94-95 8.06474080656206E-4 0.0 0.0 8.998601134049188 6.598424296278049E-4 96-97 8.06474080656206E-4 0.0 0.0 9.743489921273467 7.698161678991056E-4 98-99 8.06474080656206E-4 0.0 0.0 10.535557442216131 8.06474080656206E-4 100-101 8.06474080656206E-4 0.0 0.0 11.402920315961882 8.06474080656206E-4 102-103 8.06474080656206E-4 0.0 0.0 12.310900157042498 8.06474080656206E-4 104-105 8.06474080656206E-4 0.0 0.0 13.247289880509868 8.06474080656206E-4 106-107 8.06474080656206E-4 0.0 0.0 14.206407509886638 8.06474080656206E-4 108-109 8.06474080656206E-4 0.0 0.0 15.213217083760398 8.06474080656206E-4 110-111 8.06474080656206E-4 0.0 0.0 16.267498654654602 8.797899061704065E-4 112-113 8.06474080656206E-4 0.0 0.0 17.380836123000492 8.797899061704065E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCATAG 160 3.6379788E-12 40.907196 8 ACGCATA 165 5.456968E-12 39.6705 7 GTATCAA 5185 0.0 32.40188 1 TATCAAC 6060 0.0 27.920006 2 GGTATCA 3805 0.0 27.400166 1 ATCAACG 5995 0.0 27.030216 3 TCAACGC 6000 0.0 26.809103 4 CAACGCA 6115 0.0 26.500751 5 GTATACG 90 0.0094706165 26.478127 1 GTACATG 4190 0.0 26.16216 1 AACGCAG 6090 0.0 25.60582 6 GTTATCA 775 0.0 25.367756 1 TACATGG 4045 0.0 25.185314 2 AACGCAT 260 1.1023076E-9 25.18106 6 GAGTACT 2625 0.0 24.252558 12-13 ACATGGG 3965 0.0 24.04067 3 ACGCAGA 6580 0.0 23.241606 7 CGCAGAG 6460 0.0 22.56631 8 GTACTTT 2940 0.0 21.250103 14-15 GTTCGCG 260 7.6900506E-7 20.598145 7 >>END_MODULE