FastQCFastQC Report
Thu 26 May 2016
SRR1364639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1363962
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72200.5293402602125279No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71190.5219353618355936No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49740.3646729161076335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16650.1220708494811439No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16540.1212643754004877No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46400.049.9551661
GTATCAA69900.041.770311
TATCAAC90400.032.219132
ATCAACG93950.031.4155033
TCAACGC95350.030.7670144
CAACGCA96550.029.9861835
AACGCAG99750.029.0732676
ACGCAGA111950.025.7985577
CGCAGAG112800.025.6569448
GAGTACT76350.024.60572812-13
AGTACTT78550.024.3714112-13
AGAGTAC97850.024.14300310-11
GTACATG40700.023.8639321
GCAGAGT116850.023.4953469
GTACTTT82700.022.5724114-15
CAGAGTA110650.022.10398910-11
TACATGG40800.021.8980032
ACATGGG38700.021.6803993
TACTTTT93000.020.58467114-15
CGCTATA1500.004666892319.8622111
Produced by FastQC (version 0.11.5)