##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364639_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1363962 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.576463273903524 31.0 30.0 33.0 26.0 34.0 2 29.696838328340526 31.0 30.0 34.0 26.0 34.0 3 29.697127192693053 31.0 30.0 34.0 26.0 34.0 4 33.349072774754724 35.0 33.0 37.0 28.0 37.0 5 32.742427574961766 35.0 33.0 37.0 26.0 37.0 6 32.723191701821605 35.0 33.0 37.0 25.0 37.0 7 32.51421227277593 35.0 33.0 37.0 25.0 37.0 8 32.43251058313941 35.0 33.0 37.0 23.0 37.0 9 33.71639532479644 37.0 33.0 39.0 23.0 39.0 10-11 33.48717852843408 37.0 33.0 39.0 20.5 39.0 12-13 33.36036891057083 37.0 33.0 39.0 19.0 39.0 14-15 34.297334163268474 38.0 33.0 41.0 19.0 41.0 16-17 34.214166523700804 38.0 33.0 41.0 18.5 41.0 18-19 34.26564743006037 38.5 33.0 41.0 18.5 41.0 20-21 34.26900786092281 39.0 33.0 41.0 18.0 41.0 22-23 34.23749305332554 39.0 33.0 41.0 19.0 41.0 24-25 34.19464435226202 39.0 33.0 41.0 18.5 41.0 26-27 34.054795514831056 38.0 33.0 41.0 18.0 41.0 28-29 33.77989122864126 38.0 33.0 41.0 17.0 41.0 30-31 33.64494941941197 38.0 32.5 40.0 16.5 41.0 32-33 33.39316747827286 38.0 32.0 40.0 15.5 41.0 34-35 33.20554971472812 38.0 32.0 40.0 12.5 41.0 36-37 32.986822946680334 38.0 32.0 40.0 9.0 41.0 38-39 32.82940213876927 38.0 31.5 40.0 8.5 41.0 40-41 32.630182878995164 38.0 31.0 40.0 7.5 41.0 42-43 32.421733523367955 38.0 31.0 40.0 2.0 41.0 44-45 32.15859826006883 38.0 31.0 40.0 2.0 41.0 46-47 31.82984020082671 37.0 30.0 40.0 2.0 41.0 48-49 31.494772581640838 37.0 30.0 40.0 2.0 41.0 50-51 31.257109435600114 37.0 30.0 40.0 2.0 41.0 52-53 31.0670069987287 37.0 29.0 40.0 2.0 41.0 54-55 30.828650651557744 36.0 29.0 40.0 2.0 41.0 56-57 30.57621070088463 36.0 28.0 40.0 2.0 41.0 58-59 30.372541537080945 36.0 28.0 40.0 2.0 41.0 60-61 30.13409207881158 35.0 28.0 39.0 2.0 41.0 62-63 29.855388933122768 35.0 28.0 39.0 2.0 41.0 64-65 29.572397178220506 35.0 28.0 39.0 2.0 41.0 66-67 29.277515795894608 35.0 27.5 38.5 2.0 40.0 68-69 28.952214577825483 35.0 26.5 38.0 2.0 40.0 70-71 28.669594167579447 34.0 26.0 37.0 2.0 40.0 72-73 28.497246257593687 34.0 26.0 37.0 2.0 39.0 74-75 28.21779455732638 34.0 26.0 36.0 2.0 39.0 76-77 27.935572252012886 34.0 26.0 36.0 2.0 39.0 78-79 27.646129437623628 34.0 26.0 35.5 2.0 37.5 80-81 27.385468950014737 34.0 26.0 35.0 2.0 37.0 82-83 27.141958866889254 34.0 25.5 35.0 2.0 36.5 84-85 27.1475689938576 34.0 26.0 35.0 2.0 36.0 86-87 27.022802688051428 34.0 26.0 35.0 2.0 36.0 88-89 26.89320157013172 34.0 25.5 35.0 2.0 36.0 90-91 26.751986492292307 34.0 25.0 35.0 2.0 35.0 92-93 26.623512238610754 34.0 25.0 35.0 2.0 35.0 94-95 26.538647704261557 34.0 25.0 35.0 2.0 35.0 96-97 26.460072934583224 34.0 25.0 35.0 2.0 35.0 98-99 26.367081707554902 34.0 24.0 35.0 2.0 35.0 100-101 26.263228374397528 34.0 24.0 35.0 2.0 35.0 102-103 25.06413265178942 31.5 21.5 34.0 2.0 35.0 104-105 25.99332349434955 33.0 23.5 35.0 2.0 35.0 106-107 26.157038099301886 34.0 24.0 35.0 2.0 35.0 108-109 26.157679246196007 34.0 24.0 35.0 2.0 35.0 110-111 26.07871553606332 34.0 24.0 35.0 2.0 35.0 112-113 26.00895699440307 34.0 23.0 35.0 2.0 35.0 114-115 25.903150527653995 34.0 23.0 35.0 2.0 35.0 116-117 25.756640947475077 34.0 20.0 35.0 2.0 35.0 118-119 25.6224638223059 34.0 19.5 35.0 2.0 35.0 120-121 25.45482462121379 34.0 18.5 35.0 2.0 35.0 122-123 25.236383784885504 33.0 8.5 35.0 2.0 35.0 124-125 24.382850475306498 32.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8002463448362995 1101 2 0.7776039384560001 1101 3 0.5703742152442608 1101 4 0.5490472616016149 1101 5 0.8198344258404617 1101 6 0.8867539311044084 1101 7 0.9099497786481834 1101 8 1.0636883157261963 1101 9 0.9706578221967206 1101 10-11 1.152217381643844 1101 12-13 1.0647044685385225 1101 14-15 1.2138825771351947 1101 16-17 1.3010044385089614 1101 18-19 1.2268127592542868 1101 20-21 1.2931720505306075 1101 22-23 1.3165798319571778 1101 24-25 1.1300329061481662 1101 26-27 1.3277923292614204 1101 28-29 1.3413273477181349 1101 30-31 1.2247262190550074 1101 32-33 1.2116206609219518 1101 34-35 1.1093771471186002 1101 36-37 1.1935418150624884 1101 38-39 1.2109023185244325 1101 40-41 1.2597620110855416 1101 42-43 1.3558004551084721 1101 44-45 1.2619119995410202 1101 46-47 1.3047723295532805 1101 48-49 1.1896414331087257 1101 50-51 1.1927595961442563 1101 52-53 1.1342147588856655 1101 54-55 1.1235338978847373 1101 56-57 1.3258784705858915 1101 58-59 1.2414451979715153 1101 60-61 1.1526932321953716 1101 62-63 1.1774968919108169 1101 64-65 1.2253195550314508 1101 66-67 1.2176111174420114 1101 68-69 1.3278830927277383 1101 70-71 1.3000946724845015 1101 72-73 1.1969242357737073 1101 74-75 1.1279474334131088 1101 76-77 1.125870472644241 1101 78-79 1.013226857071217 1101 80-81 1.0352039262776458 1101 82-83 1.0968718561030535 1101 84-85 1.2168249835832263 1101 86-87 1.09904506927262 1101 88-89 0.9472850409265874 1101 90-91 0.9837107683792716 1101 92-93 1.1312951929765482 1101 94-95 1.0362456781167388 1101 96-97 1.1510453853179996 1101 98-99 1.1579698878973943 1101 100-101 1.1494920223611444 1101 102-103 1.0654376073774756 1101 104-105 1.1192345171724298 1101 106-107 1.0630757171717455 1101 108-109 1.0833555160617756 1101 110-111 0.9935910327577915 1101 112-113 0.9330467828035474 1101 114-115 0.9240600706437512 1101 116-117 0.9538999447903187 1101 118-119 0.9914035526552709 1101 120-121 1.0440007767505648 1101 122-123 1.0586003862377815 1101 124-125 0.8912022689281294 1102 1 0.5714757985001135 1102 2 -0.43950779808258744 1102 3 0.1523668923470467 1102 4 0.39910831968034444 1102 5 0.7497634722298372 1102 6 0.6589378149276044 1102 7 0.6119662477859862 1102 8 0.671051019038913 1102 9 0.7972643317667334 1102 10-11 0.8433077888317442 1102 12-13 1.0469911355818695 1102 14-15 1.0845524547594323 1102 16-17 1.1543432642697198 1102 18-19 1.101843110133359 1102 20-21 1.0997959739970185 1102 22-23 1.0444263626417296 1102 24-25 1.1990686211792436 1102 26-27 1.0877988295670917 1102 28-29 1.2563919715479415 1102 30-31 1.197753259206742 1102 32-33 0.9783897443075915 1102 34-35 1.0578394456002087 1102 36-37 1.012369464318823 1102 38-39 0.9192844550532442 1102 40-41 0.8877848186576642 1102 42-43 0.7949840380952864 1102 44-45 0.5938577186799634 1102 46-47 0.601998042868928 1102 48-49 0.6733999587151942 1102 50-51 0.5487369758949612 1102 52-53 0.6931321143963949 1102 54-55 0.6896360763779406 1102 56-57 0.5977084517059907 1102 58-59 0.7668210780197526 1102 60-61 0.7060867277156575 1102 62-63 0.7920262204042992 1102 64-65 0.9431897454033624 1102 66-67 0.912442636339982 1102 68-69 0.5334936913310635 1102 70-71 0.38389453127973994 1102 72-73 0.6313438124342987 1102 74-75 0.6714034021139099 1102 76-77 0.7943793167758706 1102 78-79 0.7860122864095338 1102 80-81 0.7286759548565946 1102 82-83 0.6249430469213841 1102 84-85 0.7863176797746192 1102 86-87 0.6972504606068028 1102 88-89 0.8407434020656481 1102 90-91 0.7625376133691226 1102 92-93 0.7597658191794459 1102 94-95 0.5519786321213509 1102 96-97 0.5504083604524226 1102 98-99 0.5153762404827305 1102 100-101 0.39451485996599445 1102 102-103 0.3878031383486089 1102 104-105 0.53860642068285 1102 106-107 0.7995088105995158 1102 108-109 0.8203088375550855 1102 110-111 0.6777212261940591 1102 112-113 0.7061222316529623 1102 114-115 0.7873481309006181 1102 116-117 0.7276459126410373 1102 118-119 0.6766089434804208 1102 120-121 0.5447326337967802 1102 122-123 0.5167913822200134 1102 124-125 0.4936441848537818 1103 1 0.32037992048867636 1103 2 -1.041152070246504 1103 3 2.512035279416125E-4 1103 4 0.2976921337384084 1103 5 0.4234260651406814 1103 6 0.6065800393157303 1103 7 0.6518045386291291 1103 8 0.7389291709943322 1103 9 0.6935034348481111 1103 10-11 0.8063650006514109 1103 12-13 0.8767763755442175 1103 14-15 1.0819706828359088 1103 16-17 1.0960320408121476 1103 18-19 1.1387811545305553 1103 20-21 0.9905235703272268 1103 22-23 1.0757457164307667 1103 24-25 1.0463956019458962 1103 26-27 0.8974342736787904 1103 28-29 1.0892896460941586 1103 30-31 1.066328285727046 1103 32-33 1.0660431896660043 1103 34-35 1.054616621900479 1103 36-37 1.082032047998517 1103 38-39 1.0343769677992825 1103 40-41 1.2004032048944353 1103 42-43 1.1393670526848538 1103 44-45 1.3671659125268434 1103 46-47 1.3504395818957775 1103 48-49 1.2525271590292633 1103 50-51 1.337551699367026 1103 52-53 1.286751315794607 1103 54-55 1.2193585701924334 1103 56-57 1.2011697210244066 1103 58-59 1.1996600065811975 1103 60-61 1.1041910350451083 1103 62-63 1.216018890874711 1103 64-65 1.2374485369797341 1103 66-67 1.1493547309612744 1103 68-69 1.0299381617594747 1103 70-71 0.9990629445560764 1103 72-73 0.9544585240340844 1103 74-75 1.0751402467698838 1103 76-77 1.0002019387750778 1103 78-79 0.9194499060458234 1103 80-81 0.9443848773485612 1103 82-83 0.9787168289807049 1103 84-85 1.0549518369107673 1103 86-87 0.9776608670823492 1103 88-89 1.0169864847964583 1103 90-91 1.1150077525363393 1103 92-93 1.1183883886636927 1103 94-95 1.0953575801348734 1103 96-97 1.0641327661397355 1103 98-99 1.0857370577570755 1103 100-101 1.0384922682757178 1103 102-103 1.023730346663001 1103 104-105 0.9632238602143595 1103 106-107 0.9979517727613079 1103 108-109 1.003663235849782 1103 110-111 1.0442546310174876 1103 112-113 0.8054928213770047 1103 114-115 0.8081060284661881 1103 116-117 0.8086090852421748 1103 118-119 0.862058298979786 1103 120-121 0.846697252354307 1103 122-123 1.0058693984776 1103 124-125 0.7896736896464098 1104 1 0.344912409224861 1104 2 -0.547688089313727 1104 3 0.13765541661862457 1104 4 0.2137448360117773 1104 5 0.4556116933323011 1104 6 0.5132306285424519 1104 7 0.6662840478664975 1104 8 0.6201952325899001 1104 9 0.6555188883263696 1104 10-11 0.7649321867273287 1104 12-13 0.6605157541912305 1104 14-15 0.6372808379962862 1104 16-17 0.9476552202935409 1104 18-19 0.8202956150265379 1104 20-21 0.8793959129189872 1104 22-23 0.8807941634163114 1104 24-25 0.7815295104197872 1104 26-27 0.7782304013545982 1104 28-29 0.9531090275870255 1104 30-31 0.7109377842556697 1104 32-33 0.741729046629942 1104 34-35 0.8538374466829595 1104 36-37 0.9600616865873235 1104 38-39 0.8182197539885436 1104 40-41 0.857803836134309 1104 42-43 0.9967594652944456 1104 44-45 0.9885786337398628 1104 46-47 0.7721919656052236 1104 48-49 0.7056505452030706 1104 50-51 0.5775158317924607 1104 52-53 0.36320665735229696 1104 54-55 0.5631268244449608 1104 56-57 0.7366387533481529 1104 58-59 0.6251741923050673 1104 60-61 0.6030633653702608 1104 62-63 0.6046152346882288 1104 64-65 0.5774406372813701 1104 66-67 0.4938665872554573 1104 68-69 0.5387699999972035 1104 70-71 0.5622382062335838 1104 72-73 0.5434664126924922 1104 74-75 0.49674800711952827 1104 76-77 0.49051057900883066 1104 78-79 0.512725594478777 1104 80-81 0.49566189302897357 1104 82-83 0.6264311297654004 1104 84-85 0.6254062290326594 1104 86-87 0.6249993188641341 1104 88-89 0.6525134155305778 1104 90-91 0.6362810023667507 1104 92-93 0.7115405523101117 1104 94-95 0.7030329221586946 1104 96-97 0.6733209494667065 1104 98-99 0.6518634210377066 1104 100-101 0.6465158594246248 1104 102-103 0.7331091531461489 1104 104-105 0.6070582315075406 1104 106-107 0.665026099845619 1104 108-109 0.6254227341944052 1104 110-111 0.6269195770873282 1104 112-113 0.7308629970716929 1104 114-115 0.7452181735164238 1104 116-117 0.8463094143553889 1104 118-119 0.6161319796136304 1104 120-121 0.7408847180844553 1104 122-123 0.8030571549264636 1104 124-125 0.7168746572367972 1105 1 0.6466971370086263 1105 2 0.47396499897392985 1105 3 0.6700829989258388 1105 4 0.6158549953679682 1105 5 0.670399370228786 1105 6 0.5764059936918215 1105 7 0.6974904632809071 1105 8 0.7630001901276486 1105 9 0.8576510506896327 1105 10-11 1.0507022742306873 1105 12-13 0.9997800020255383 1105 14-15 1.16618403541176 1105 16-17 1.1942646870281877 1105 18-19 1.179077594800333 1105 20-21 1.3083089288806349 1105 22-23 1.1238355941322737 1105 24-25 1.2068104923690441 1105 26-27 1.2322033045330798 1105 28-29 1.4840832284697498 1105 30-31 1.1927086477790638 1105 32-33 1.1767276684846024 1105 34-35 1.2911757216235458 1105 36-37 1.2253951208663523 1105 38-39 1.2823591110985362 1105 40-41 1.2619396916904861 1105 42-43 1.2498287073381746 1105 44-45 1.2764332104244787 1105 46-47 1.137597915171014 1105 48-49 1.1982549323187754 1105 50-51 1.0497297010212563 1105 52-53 0.9544422444954428 1105 54-55 0.9951027536284016 1105 56-57 0.932336692281531 1105 58-59 0.8353000707987874 1105 60-61 0.8919435660944686 1105 62-63 0.8430910414355814 1105 64-65 0.8775305420414732 1105 66-67 0.8757184833586784 1105 68-69 0.8711903662644644 1105 70-71 0.8780284395009481 1105 72-73 0.8102405707521605 1105 74-75 0.8323157497647884 1105 76-77 0.903406352183854 1105 78-79 0.9362016200732377 1105 80-81 0.6536832312140106 1105 82-83 0.7586021286191169 1105 84-85 0.6149049981655139 1105 86-87 0.6251346491872383 1105 88-89 0.6557232520576974 1105 90-91 0.6261346587105372 1105 92-93 0.5074077724431021 1105 94-95 0.593534484253901 1105 96-97 0.6746233855308184 1105 98-99 0.6573844013781383 1105 100-101 0.654438834704365 1105 102-103 0.38311820277002795 1105 104-105 0.28875374064648085 1105 106-107 0.5876352542795225 1105 108-109 0.6410122195961172 1105 110-111 0.6455803747081603 1105 112-113 0.47417804582727996 1105 114-115 0.6144179856627936 1105 116-117 0.2927552780857958 1105 118-119 0.2869944383432035 1105 120-121 0.3522896100863626 1105 122-123 0.30814588264398424 1105 124-125 0.29936606009411193 1106 1 0.4815670948053281 1106 2 0.4777682796973295 1106 3 0.6792183485135865 1106 4 0.3063366376856109 1106 5 0.5698402329514636 1106 6 0.518285479202774 1106 7 0.4036655166932803 1106 8 0.5172943380874457 1106 9 0.47665414823116237 1106 10-11 0.7110477714171353 1106 12-13 0.5807395957529664 1106 14-15 0.7548684406913111 1106 16-17 0.9861213453319309 1106 18-19 0.8614097360723036 1106 20-21 0.825354575772181 1106 22-23 0.8572446106675358 1106 24-25 0.9139933752078733 1106 26-27 0.7801919243122128 1106 28-29 0.8665534379064397 1106 30-31 0.7931061609064685 1106 32-33 0.7012121403415534 1106 34-35 0.6654663385364614 1106 36-37 0.69877039263001 1106 38-39 0.7288029801479468 1106 40-41 0.911515613430069 1106 42-43 0.9022029014507709 1106 44-45 0.7955315427923821 1106 46-47 0.602163436543492 1106 48-49 0.5307448422700247 1106 50-51 0.7242355895473764 1106 52-53 0.8736867086038309 1106 54-55 0.8081659109391239 1106 56-57 0.7307042766128973 1106 58-59 0.5978375205836315 1106 60-61 0.8217849322851869 1106 62-63 0.9090305208906244 1106 64-65 0.7264969467868987 1106 66-67 0.520557935919463 1106 68-69 0.5405624840650773 1106 70-71 0.6684702106101206 1106 72-73 0.8557707402800254 1106 74-75 0.7766513346302801 1106 76-77 0.7649955442652789 1106 78-79 0.6170698579538971 1106 80-81 0.5248626713996742 1106 82-83 0.5359973632574153 1106 84-85 0.45704169528726624 1106 86-87 0.539115925705353 1106 88-89 0.518708876794399 1106 90-91 0.6927069995642334 1106 92-93 0.7434556705533559 1106 94-95 0.6567035494621329 1106 96-97 0.5022035652145576 1106 98-99 0.4984800038847581 1106 100-101 0.6180784554012817 1106 102-103 0.5793923370758165 1106 104-105 0.6482768471402203 1106 106-107 0.5754714795292806 1106 108-109 0.6574508649530024 1106 110-111 0.6796465138962944 1106 112-113 0.5631645413361284 1106 114-115 0.404778768379753 1106 116-117 0.6753526710353626 1106 118-119 0.7719978330380748 1106 120-121 0.65527905738114 1106 122-123 0.6261005507725947 1106 124-125 0.5353543629723596 1107 1 0.454306019179743 1107 2 0.6091036033058437 1107 3 0.5080033732154092 1107 4 0.40157980083458256 1107 5 0.42041794869047067 1107 6 0.3355408544074052 1107 7 0.28250325201835125 1107 8 0.20772037147145284 1107 9 0.4594893951032404 1107 10-11 0.5167817188855608 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-2.523449576828053 2216 76-77 -2.8161079713597204 2216 78-79 -2.3758826160044357 2216 80-81 -2.0405507400586593 2216 82-83 -1.9988268554925845 2216 84-85 -2.0125058307543995 2216 86-87 -2.0421177982529812 2216 88-89 -2.039347763830598 2216 90-91 -1.9106881827274336 2216 92-93 -2.038757871113308 2216 94-95 -1.9167469713712002 2216 96-97 -1.8823895337437762 2216 98-99 -1.886122860698574 2216 100-101 -2.0818824820987203 2216 102-103 -1.920461178549182 2216 104-105 -1.8258507570264477 2216 106-107 -2.0090402392207203 2216 108-109 -1.9813186662969962 2216 110-111 -1.992521454853705 2216 112-113 -1.97778923470554 2216 114-115 -2.0428351639119136 2216 116-117 -2.0003439435479713 2216 118-119 -2.194401906545256 2216 120-121 -2.1866935988688603 2216 122-123 -2.229430048185737 2216 124-125 -2.2463481109859735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 53666.0 3 17991.0 4 8622.0 5 7517.0 6 8743.0 7 10077.0 8 10865.0 9 11286.0 10 11996.0 11 12847.0 12 14247.0 13 15887.0 14 18228.0 15 15307.0 16 9959.0 17 8509.0 18 7872.0 19 7410.0 20 7205.0 21 7604.0 22 8443.0 23 10601.0 24 14192.0 25 18467.0 26 21433.0 27 24725.0 28 28387.0 29 33279.0 30 38405.0 31 45222.0 32 55028.0 33 67538.0 34 86349.0 35 117572.0 36 181588.0 37 259451.0 38 87292.0 39 152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.672761470763646 19.761690103236322 14.099060801080999 24.466487624919033 2 16.40498375166647 21.93653428071867 36.95536532519779 24.703116642417065 3 18.54685101197834 25.190364540947623 31.055337318781607 25.207447128292433 4 13.561386207579195 18.40820022509559 35.631605767432994 32.39880779989222 5 13.355764548510681 37.6989621407241 33.9655435413941 14.979729769371126 6 30.71755980398195 37.03873145468036 16.90758352908745 15.336125212250248 7 26.09634732957907 33.95727590451633 21.549369813359768 18.39700695254483 8 25.439345084393842 34.169866902450366 20.461200531979628 19.92958748117616 9 25.443875916363563 16.31986779583724 20.904709919931143 37.33154636786805 10-11 24.249568171339046 26.882943529025965 27.53777514977824 21.32971314985674 12-13 25.399213030565548 25.013133876823485 27.746043431063555 21.841609661547416 14-15 22.629489695888108 26.286428428453384 26.624546420377847 24.45953545528066 16-17 21.31618095819347 28.602468251918967 27.759691673904367 22.32165911598319 18-19 21.155825684037655 26.73648787354468 30.83763453473709 21.270051907680575 20-21 21.933705202068435 26.006566055582336 30.946835480163436 21.11289326218579 22-23 21.813027110973586 25.747359220766654 30.839887315020803 21.59972635323896 24-25 21.771427649743906 25.939395672313452 30.913471196411628 21.37570548153101 26-27 21.47647808370028 26.008899074900917 30.90031100573183 21.614311835666978 28-29 21.411794478696365 26.10750661096705 30.92920305296476 21.551495857371826 30-31 21.78352476095375 25.723847145301704 30.723363260853308 21.76926483289124 32-33 21.581288542597658 25.814366458857712 30.537175025093244 22.067169973451385 34-35 22.096216756771817 25.914541607464137 30.33178343678196 21.657458198982084 36-37 22.27557176325854 25.451250237818467 30.38713364499925 21.88604435392374 38-39 21.85394461136014 25.689058786095213 30.420129006526576 22.03686759601807 40-41 22.195742989907345 25.614716539023814 30.296041971843792 21.893498499225053 42-43 22.142541612670854 25.66522535270645 29.813570102297245 22.37866293232545 44-45 22.145301701953574 25.76123821631394 29.793205382554643 22.300254699177835 46-47 22.45856003214168 25.993081913632427 29.139469530287155 22.40888852393873 48-49 22.410314386282874 26.103130665951102 28.83986982013389 22.646685127632136 50-51 22.45874410217628 26.286028916611343 28.56315432346999 22.692072657742386 52-53 22.720077773484725 26.197193321509925 28.32331716229423 22.75941174271112 54-55 22.451981800079473 26.289845318271333 28.30012859595795 22.958044285691244 56-57 22.307681310145128 26.43740691034543 28.26846185739041 22.986449922119036 58-59 22.37228749774554 26.047536514946895 28.442691218670312 23.137484768637247 60-61 22.19328690975262 26.24057708352579 28.564285515285615 23.00185049143598 62-63 22.1841590894761 26.114070626601034 28.706958111736252 22.994812172186617 64-65 22.12735913381243 26.185633826360714 28.414631285219293 23.27237575460756 66-67 22.065900662921695 26.243912953586683 28.271902003134986 23.41828438035664 68-69 22.268423614838014 25.96894621234116 28.427090023431838 23.335540149388983 70-71 22.278113319872546 25.859481422502974 28.18865188326361 23.67375337436087 72-73 22.336229710058063 25.999865830416248 28.136168197336332 23.52773626218936 74-75 22.102184975238803 26.333565139228337 27.960373289362312 23.603876596170547 76-77 22.241479930437638 26.029725299056565 28.10559871432803 23.623196056177765 78-79 22.25880300083764 26.15113062954905 28.05346960396348 23.536596765649833 80-81 22.327155153452498 26.05231901696556 27.95064299016086 23.66988283942109 82-83 22.268781878660754 25.992312096900132 28.034379282105576 23.70452674233354 84-85 22.133990634629583 25.980618214157147 27.971071020359084 23.91432013085418 86-87 22.103283238926714 25.93921513712299 27.98539251208615 23.97210911186415 88-89 22.152083415813635 25.79745623411796 27.849896111475246 24.20056423859316 90-91 21.86178940469016 25.98983696026722 27.985603704502033 24.162769930540588 92-93 21.998120818261572 25.857408745490385 27.9190830062362 24.225387430011843 94-95 21.882665280447306 25.66230039339351 28.046160224913603 24.40887410124558 96-97 21.82694613017533 25.516295561474863 28.096911868107536 24.559846440242275 98-99 21.699396241078333 25.72383253113189 27.84314731791005 24.733623909879725 100-101 21.57682448083938 25.683590349105707 27.920575179633662 24.819009990421247 102-103 21.45574023852623 25.413373862410822 28.080792751101757 25.050093147961196 104-105 21.440220827995006 25.466482643337496 27.98525613884597 25.10804038982153 106-107 21.30731068553051 25.615535284653106 27.841444606681726 25.23570942313466 108-109 21.154316378104067 25.647813225965976 27.706371839017883 25.491498556912067 110-111 21.119640633437086 25.676057613316562 27.80160349806134 25.402698255185012 112-113 21.15713628473654 25.646891055426334 27.77680631851766 25.41916634131946 114-115 20.931202717975246 25.747448690629067 27.604472723287 25.716875868108698 116-117 20.722355822103612 25.71319119609367 27.74960775400677 25.814845227795946 118-119 20.698631462720446 25.660220625864227 27.63347747478258 26.00767043663275 120-121 20.568913590611714 25.744604797894162 27.57852441016184 26.107957201332283 122-123 20.53058085366265 25.717470772560446 27.475552766287525 26.276395607489377 124-125 20.466390286694427 25.586177140035492 27.437060259460893 26.51037231380919 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 390.0 1 298.5 2 401.0 3 676.0 4 579.0 5 487.5 6 904.5 7 2209.5 8 3408.0 9 3716.0 10 3016.0 11 1964.0 12 1664.5 13 1661.0 14 1747.5 15 1877.5 16 2001.5 17 2084.0 18 2238.5 19 2394.0 20 2586.5 21 2916.0 22 3291.0 23 3728.5 24 4339.5 25 5214.0 26 6563.0 27 8291.5 28 9941.0 29 11828.5 30 14194.0 31 17215.0 32 20997.0 33 23608.0 34 25283.0 35 27823.0 36 30956.0 37 34045.0 38 36391.5 39 37907.5 40 39139.0 41 40757.0 42 42401.0 43 44223.0 44 46439.5 45 48848.5 46 49848.5 47 50067.5 48 51345.5 49 51357.0 50 49608.0 51 48161.5 52 47157.5 53 46718.0 54 45819.0 55 42941.5 56 39362.0 57 35645.5 58 32736.5 59 30096.5 60 25883.0 61 21528.5 62 17803.5 63 13328.0 64 9612.5 65 7505.5 66 5689.0 67 4567.5 68 3759.5 69 2902.5 70 2201.5 71 1525.0 72 1117.5 73 877.0 74 667.5 75 521.0 76 369.0 77 236.5 78 166.0 79 111.5 80 71.0 81 58.5 82 44.5 83 30.0 84 21.0 85 17.0 86 14.0 87 6.5 88 2.5 89 1.0 90 2.5 91 2.0 92 1.0 93 1.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05711302807556222 2 0.07720156426645318 3 0.0 4 0.007111635074877452 5 0.02829990864848141 6 4.3989495308520323E-4 7 7.331582551420053E-5 8 0.0 9 8.064740806562058E-4 10-11 0.06330821533151217 12-13 0.008981188625489567 14-15 0.006525108470763849 16-17 0.0535205526253664 18-19 1.4663165102840107E-4 20-21 0.00949439940408897 22-23 0.011730532082272087 24-25 0.0 26-27 0.0 28-29 0.05110113038339777 30-31 0.0 32-33 0.0035924754501958263 34-35 0.0 36-37 1.0997373827130081E-4 38-39 0.0 40-41 0.0 42-43 0.0032625542353819244 44-45 0.0 46-47 7.331582551420053E-5 48-49 2.1994747654260162E-4 50-51 4.032370403281029E-4 52-53 7.331582551420053E-5 54-55 0.0 56-57 0.0015762902485553115 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.865266041136043E-4 66-67 0.0 68-69 4.3989495308520323E-4 70-71 0.0 72-73 0.001209711120984309 74-75 0.002456080154725718 76-77 4.3989495308520323E-4 78-79 6.965003423849052E-4 80-81 0.0016129481613124117 82-83 7.331582551420053E-5 84-85 7.331582551420053E-5 86-87 2.9326330205680214E-4 88-89 0.0 90-91 0.0 92-93 0.005168765698751138 94-95 0.004435607443609133 96-97 0.004618897007394634 98-99 5.132107785994039E-4 100-101 4.032370403281029E-4 102-103 2.1994747654260162E-4 104-105 3.2992121481390245E-4 106-107 0.0034825017119245257 108-109 0.0034091858864103253 110-111 4.7655286584230355E-4 112-113 0.0025293959802399186 114-115 4.032370403281029E-4 116-117 0.0016129481613124117 118-119 0.001759579812340813 120-121 0.009860978531659972 122-123 2.9326330205680214E-4 124-125 0.00135634277201271 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1363962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.49450554764508 #Duplication Level Percentage of deduplicated Percentage of total 1 82.10422797031049 51.31063130375686 2 10.290703727339071 12.862248823547272 3 3.1292358814239036 5.866801474546086 4 1.3821795383537194 3.455145073099522 5 0.783539935206618 2.4483470413785726 6 0.4809140671934036 1.8032692104095236 7 0.33987717937071366 1.486831939019072 8 0.2372606235510607 1.1861988283799596 9 0.17620155199288182 0.9910465981670544 >10 0.9913849623813942 11.127812678790086 >50 0.06021043938530354 2.5567931344492663 >100 0.022252002811882304 2.6220573762484536 >500 0.0013019604397307301 0.5313898602426383 >1k 4.7344015990208365E-4 0.69079202461108 >5k 2.3672007995104182E-4 1.0606346333545644 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7220 0.5293402602125279 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7119 0.5219353618355936 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4974 0.3646729161076335 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1665 0.1220708494811439 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1654 0.1212643754004877 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 7.331582551420055E-5 5 0.0 0.0 0.0 0.0 7.331582551420055E-5 6 0.0 0.0 0.0 7.331582551420055E-5 7.331582551420055E-5 7 0.0 0.0 0.0 7.331582551420055E-5 7.331582551420055E-5 8 0.0 0.0 0.0 7.331582551420055E-5 7.331582551420055E-5 9 0.0 0.0 0.0 7.331582551420055E-5 7.331582551420055E-5 10-11 0.0 0.0 0.0 7.331582551420055E-5 7.331582551420055E-5 12-13 0.0 0.0 0.0 7.331582551420055E-5 7.331582551420055E-5 14-15 0.0 3.6657912757100274E-5 0.0 7.331582551420055E-5 7.331582551420055E-5 16-17 0.0 7.331582551420055E-5 0.0 7.331582551420055E-5 1.0997373827130082E-4 18-19 0.0 7.331582551420055E-5 0.0 7.331582551420055E-5 2.1994747654260162E-4 20-21 0.0 1.0997373827130082E-4 0.0 1.0997373827130082E-4 2.932633020568022E-4 22-23 0.0 1.466316510284011E-4 0.0 6.598424296278048E-4 2.932633020568022E-4 24-25 7.331582551420055E-5 1.466316510284011E-4 0.0 0.0018695535506121138 3.2992121481390245E-4 26-27 7.331582551420055E-5 1.466316510284011E-4 0.0 0.0032992121481390246 3.665791275710027E-4 28-29 1.0997373827130082E-4 1.466316510284011E-4 0.0 0.00813805663207626 3.665791275710027E-4 30-31 1.466316510284011E-4 1.466316510284011E-4 0.0 0.019575325412291546 4.03237040328103E-4 32-33 1.466316510284011E-4 1.466316510284011E-4 0.0 0.04409946904679163 4.3989495308520323E-4 34-35 2.1994747654260164E-4 1.466316510284011E-4 0.0 0.08229701413969011 4.3989495308520323E-4 36-37 2.932633020568022E-4 1.466316510284011E-4 0.0 0.14179280654446386 5.132107785994038E-4 38-39 2.932633020568022E-4 1.466316510284011E-4 0.0 0.22471300520102466 5.132107785994038E-4 40-41 2.932633020568022E-4 1.466316510284011E-4 0.0 0.312582022079794 5.132107785994038E-4 42-43 3.2992121481390245E-4 1.466316510284011E-4 0.0 0.39887474871000805 5.132107785994038E-4 44-45 3.665791275710027E-4 1.466316510284011E-4 0.0 0.507455486296539 5.132107785994038E-4 46-47 4.3989495308520323E-4 1.466316510284011E-4 0.0 0.6340719169595634 6.598424296278049E-4 48-49 4.3989495308520323E-4 1.466316510284011E-4 0.0 0.7547497657559374 6.598424296278049E-4 50-51 4.3989495308520323E-4 1.466316510284011E-4 0.0 0.8771505364518952 6.598424296278049E-4 52-53 4.3989495308520323E-4 1.466316510284011E-4 0.0 0.998414911852383 7.698161678991056E-4 54-55 4.3989495308520323E-4 1.466316510284011E-4 0.0 1.135845426778752 8.797899061704065E-4 56-57 5.132107785994038E-4 1.466316510284011E-4 0.0 1.2986798752457913 8.797899061704065E-4 58-59 5.132107785994038E-4 1.466316510284011E-4 0.0 1.4735014611844024 8.797899061704065E-4 60-61 5.132107785994038E-4 1.466316510284011E-4 0.0 1.6541149973386355 8.797899061704065E-4 62-63 5.132107785994038E-4 1.466316510284011E-4 0.0 1.8365980870434808 8.797899061704065E-4 64-65 5.132107785994038E-4 1.466316510284011E-4 0.0 2.046867874618208 8.797899061704065E-4 66-67 5.865266041136044E-4 1.466316510284011E-4 0.0 2.282651569471877 8.797899061704065E-4 68-69 5.865266041136044E-4 1.466316510284011E-4 0.0 2.5571826781098004 8.797899061704065E-4 70-71 5.865266041136044E-4 1.466316510284011E-4 0.0 2.850885875119688 9.897636444417073E-4 72-73 5.865266041136044E-4 1.466316510284011E-4 0.0 3.155696419694977 0.0010264215571988075 74-75 5.865266041136044E-4 1.466316510284011E-4 0.0 3.4914829005500154 0.0010264215571988075 76-77 5.865266041136044E-4 1.466316510284011E-4 0.0 3.857585475255176 0.0010264215571988075 78-79 5.865266041136044E-4 1.466316510284011E-4 0.0 4.278564945357715 0.0010264215571988075 80-81 6.231845168707046E-4 1.466316510284011E-4 0.0 4.738255171331753 0.0010264215571988075 82-83 6.598424296278049E-4 1.466316510284011E-4 0.0 5.220856592778977 0.0010264215571988075 84-85 6.598424296278049E-4 1.466316510284011E-4 0.0 5.731354685834356 0.0010264215571988075 86-87 6.598424296278049E-4 1.466316510284011E-4 0.0 6.277410954264122 0.0010264215571988075 88-89 6.598424296278049E-4 1.466316510284011E-4 0.0 6.863204400122584 0.0010264215571988075 90-91 7.331582551420054E-4 1.466316510284011E-4 0.0 7.5127092983528865 0.0010264215571988075 92-93 7.331582551420054E-4 1.466316510284011E-4 0.0 8.186408419002877 0.0010264215571988075 94-95 7.331582551420054E-4 1.466316510284011E-4 0.0 8.886794500140033 0.0010264215571988075 96-97 7.331582551420054E-4 1.466316510284011E-4 0.0 9.624828257678733 0.0011363952954701086 98-99 7.331582551420054E-4 1.466316510284011E-4 0.0 10.407071458002495 0.0011730532082272088 100-101 7.331582551420054E-4 1.466316510284011E-4 0.0 11.265416485209998 0.0011730532082272088 102-103 7.331582551420054E-4 1.466316510284011E-4 0.0 12.166431322866767 0.0011730532082272088 104-105 8.431319934133063E-4 1.466316510284011E-4 0.0 13.09655254325267 0.0011730532082272088 106-107 8.797899061704065E-4 1.466316510284011E-4 0.0 14.047752063473908 0.0011730532082272088 108-109 9.164478189275068E-4 1.466316510284011E-4 0.0 15.047523318098305 0.0011730532082272088 110-111 9.531057316846071E-4 1.466316510284011E-4 0.0 16.09571967547483 0.001209711120984309 112-113 9.531057316846071E-4 1.466316510284011E-4 0.0 17.200955745101403 0.0012463690337414092 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4640 0.0 49.955166 1 GTATCAA 6990 0.0 41.77031 1 TATCAAC 9040 0.0 32.21913 2 ATCAACG 9395 0.0 31.415503 3 TCAACGC 9535 0.0 30.767014 4 CAACGCA 9655 0.0 29.986183 5 AACGCAG 9975 0.0 29.073267 6 ACGCAGA 11195 0.0 25.798557 7 CGCAGAG 11280 0.0 25.656944 8 GAGTACT 7635 0.0 24.605728 12-13 AGTACTT 7855 0.0 24.37141 12-13 AGAGTAC 9785 0.0 24.143003 10-11 GTACATG 4070 0.0 23.863932 1 GCAGAGT 11685 0.0 23.495346 9 GTACTTT 8270 0.0 22.57241 14-15 CAGAGTA 11065 0.0 22.103989 10-11 TACATGG 4080 0.0 21.898003 2 ACATGGG 3870 0.0 21.680399 3 TACTTTT 9300 0.0 20.584671 14-15 CGCTATA 150 0.0046668923 19.862211 1 >>END_MODULE