FastQCFastQC Report
Thu 26 May 2016
SRR1364638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4269831
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA109350.044.5072671
ATCAACG130850.036.7836723
GGTATCA69700.035.724911
CAACGCA132750.035.6306575
TCAACGC132500.035.6058734
TATCAAC140250.034.5306782
AACGCAG135050.034.2449876
GTACATG163250.034.11821
TACATGG169750.031.3722172
ACATGGG167000.029.6416243
ACGCAGA156350.029.2706787
CGCAGAG155650.027.2567048
ACGCATA5650.026.332737
CGCATAG4850.024.5369858
GCAGAGT175850.022.839379
CATGGGG85400.022.5297814
GAGTACT61550.021.9906412-13
CATGGGA90300.021.7690034
GTTATCA26450.020.7200031
AGAGTAC147700.020.6238110-11
Produced by FastQC (version 0.11.5)