FastQCFastQC Report
Thu 26 May 2016
SRR1364638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4269831
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130860.30647583007383666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115610.27076013078737776No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73160.17134167605228404No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97150.060.5855031
GTATCAA177600.051.087131
ATCAACG232250.039.0193063
TATCAAC233250.038.6733062
TCAACGC234300.038.42444
CAACGCA237600.037.501015
AACGCAG244150.036.556556
ACGCAGA275900.032.1986357
CGCAGAG278700.031.7679548
GTACATG162700.029.5122321
GCAGAGT302250.028.3081049
TACATGG166550.028.0637262
GAGTACT180650.026.47248312-13
ACATGGG166600.026.2868273
AGAGTAC270050.025.83376310-11
AGTACTT192250.025.4792512-13
GTACTTT194750.024.4641214-15
CAGAGTA290700.023.88593110-11
CATGGGG82450.022.1558364
CATGGGA88700.020.9971894
Produced by FastQC (version 0.11.5)