FastQCFastQC Report
Thu 26 May 2016
SRR1364637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273054
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43900.049.5206641
ATCAACG49650.042.3451843
TCAACGC50350.041.0467264
GGTATCA26600.040.5279921
CAACGCA51400.040.092355
AACGCAG53950.037.7266276
TATCAAC57050.037.583342
GTACATG81150.033.1747781
ACGCAGA62550.032.0603377
TACATGG81900.031.9975952
CGCAGAG61700.030.6654978
ACATGGG82700.029.5995313
GTATAGG9600.024.196441
GCAGAGT75650.023.4372049
GTGTAGC16450.021.7241841
CATGGGA48250.020.9845714
CATGGGG44450.020.3666864
AGAGTAC69900.019.57627110-11
GTACCTG21700.019.4875281
TACCTGG19900.018.8554122
Produced by FastQC (version 0.11.5)