FastQCFastQC Report
Thu 26 May 2016
SRR1364637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2273054
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51690.22740330850036997No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43940.1933082100117287No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27430.1206746518120555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44600.056.4972081
GTATCAA69050.043.652421
ATCAACG84700.034.3525733
TCAACGC85400.033.793784
TATCAAC88650.033.1129842
CAACGCA87400.032.576145
AACGCAG90550.031.4332086
GTACATG80900.027.6860371
TACATGG81400.027.2844142
ACGCAGA108700.025.9108037
CGCAGAG110400.025.565758
ACATGGG83050.024.7179993
GTGGTAT25100.023.258081
GAGTACT73550.023.1994312-13
GTACTTT76350.022.1926214-15
GCAGAGT126200.021.753029
CAGAGTA121050.021.31278610-11
TATAGGG7700.020.8786892
TGGTATC25000.020.7209382
TACCGTG3451.2732926E-920.7114947
Produced by FastQC (version 0.11.5)