##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364637_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2273054 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.847994372329033 31.0 30.0 33.0 26.0 34.0 2 29.890685395067607 31.0 30.0 34.0 26.0 34.0 3 29.919687345747175 31.0 30.0 34.0 26.0 34.0 4 33.62849804712074 35.0 33.0 37.0 28.0 37.0 5 33.08177632383568 35.0 33.0 37.0 26.0 37.0 6 33.14037018038287 35.0 33.0 37.0 26.0 37.0 7 32.95146177785482 35.0 33.0 37.0 26.0 37.0 8 32.90365605040619 35.0 33.0 37.0 26.0 37.0 9 34.215997068261466 38.0 34.0 39.0 26.0 39.0 10-11 34.02761153056637 38.0 33.5 39.0 25.0 39.0 12-13 33.901126634035094 38.0 33.0 39.0 24.5 39.0 14-15 34.93035889160574 39.0 34.0 41.0 25.0 41.0 16-17 34.84911511121162 39.0 33.5 41.0 24.5 41.0 18-19 34.872685382749374 39.0 34.0 41.0 24.0 41.0 20-21 34.87825190250649 39.0 34.0 41.0 24.0 41.0 22-23 34.87438265874898 39.0 34.0 41.0 24.0 41.0 24-25 34.88029826832094 39.0 34.0 41.0 24.0 41.0 26-27 34.79270950008227 39.0 34.0 41.0 24.0 41.0 28-29 34.55782000779568 39.0 33.5 41.0 23.0 41.0 30-31 34.50893665526644 38.5 33.5 41.0 23.0 41.0 32-33 34.34898247028008 38.0 33.0 41.0 22.5 41.0 34-35 34.22956405787104 38.0 33.0 41.0 21.5 41.0 36-37 34.084403846103086 38.0 33.0 41.0 19.5 41.0 38-39 33.9619806656595 38.0 33.0 41.0 18.5 41.0 40-41 33.80152341299414 38.0 33.0 40.0 17.0 41.0 42-43 33.64048588374935 38.0 33.0 40.0 15.5 41.0 44-45 33.454534736086345 38.0 32.5 40.0 15.0 41.0 46-47 33.23733488073754 38.0 32.0 40.0 11.0 41.0 48-49 33.00386572426348 38.0 32.0 40.0 8.5 41.0 50-51 32.81253239034356 38.0 32.0 40.0 7.5 41.0 52-53 32.643283441572436 38.0 31.0 40.0 6.5 41.0 54-55 32.427435291902434 37.5 31.0 40.0 2.0 41.0 56-57 32.20288629306651 37.0 31.0 40.0 2.0 41.0 58-59 32.010954205223456 37.0 31.0 40.0 2.0 41.0 60-61 31.79517754527609 36.5 30.5 40.0 2.0 41.0 62-63 31.52299285454723 36.0 30.0 40.0 2.0 41.0 64-65 31.246111618993652 36.0 30.0 39.0 2.0 41.0 66-67 30.965145350704383 35.0 29.5 39.0 2.0 41.0 68-69 30.637788411537958 35.0 29.0 39.0 2.0 40.0 70-71 30.377229269520214 35.0 29.0 38.0 2.0 40.0 72-73 30.219492585745876 35.0 29.0 37.5 2.0 40.0 74-75 29.93584116347434 35.0 29.0 37.0 2.0 39.0 76-77 29.63266688780821 35.0 29.0 36.5 2.0 39.0 78-79 29.32525052198496 34.0 29.0 36.0 2.0 39.0 80-81 29.05415313494532 34.0 29.0 36.0 2.0 37.5 82-83 28.800226259472936 34.0 29.0 35.0 2.0 37.0 84-85 28.78963940143965 34.0 29.0 35.0 2.0 37.0 86-87 28.64337890784821 34.0 29.0 35.0 2.0 36.0 88-89 28.504213274299687 34.0 29.0 35.0 2.0 36.0 90-91 28.349949451266887 34.0 29.0 35.0 2.0 36.0 92-93 28.2205051001868 34.0 29.0 35.0 2.0 35.5 94-95 28.137994081970774 34.0 29.0 35.0 2.0 35.0 96-97 28.057193537856996 34.0 29.0 35.0 2.0 35.0 98-99 27.96887029520636 34.0 28.5 35.0 2.0 35.0 100-101 27.87523217662229 34.0 28.0 35.0 2.0 35.0 102-103 26.67605213074568 32.5 25.0 34.5 2.0 35.0 104-105 27.627217831164593 34.0 27.0 35.0 2.0 35.0 106-107 27.807847283874473 34.0 28.0 35.0 2.0 35.0 108-109 27.824479532822362 34.0 29.0 35.0 2.0 35.0 110-111 27.7679760357651 34.0 28.0 35.0 2.0 35.0 112-113 27.722044438891466 34.0 28.0 35.0 2.0 35.0 114-115 27.64410986276613 34.0 28.0 35.0 2.0 35.0 116-117 27.52954725228701 34.0 27.0 35.0 2.0 35.0 118-119 27.427979273699613 34.0 27.0 35.0 2.0 35.0 120-121 27.29981755822783 34.0 27.0 35.0 2.0 35.0 122-123 27.117501168032085 34.0 27.0 35.0 2.0 35.0 124-125 26.303917328844804 33.0 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7928565191226795 1101 2 0.7679456622754692 1101 3 0.5491884665070259 1101 4 0.6033482569594923 1101 5 0.994197585072655 1101 6 0.9925655598753806 1101 7 1.0865324824155138 1101 8 1.177870812162773 1101 9 1.2786621072695112 1101 10-11 1.3436759854916502 1101 12-13 1.282253536895297 1101 14-15 1.3488638348314979 1101 16-17 1.421555796483922 1101 18-19 1.4366836024348117 1101 20-21 1.4770851169127397 1101 22-23 1.3869849174231916 1101 24-25 1.2362767269909156 1101 26-27 1.2365207446154685 1101 28-29 1.4469167994128895 1101 30-31 1.4244154006020011 1101 32-33 1.4297058823367195 1101 34-35 1.4133606367705696 1101 36-37 1.4593312224486041 1101 38-39 1.4387561545516476 1101 40-41 1.4944489614380245 1101 42-43 1.5326379622131014 1101 44-45 1.4303783679531534 1101 46-47 1.41192673781525 1101 48-49 1.496426172794159 1101 50-51 1.4794511548471476 1101 52-53 1.4735547417092576 1101 54-55 1.3298013165686484 1101 56-57 1.3608676915868188 1101 58-59 1.4045814010111712 1101 60-61 1.2845786759052835 1101 62-63 1.3603605478333236 1101 64-65 1.393481943853999 1101 66-67 1.3816069073990072 1101 68-69 1.3225423713273408 1101 70-71 1.3178170903702693 1101 72-73 1.3502603592839009 1101 74-75 1.2459378432130102 1101 76-77 1.2931950006672182 1101 78-79 1.1474211683680338 1101 80-81 1.1429854948563651 1101 82-83 1.1022973090159738 1101 84-85 1.1660123440255283 1101 86-87 1.113397533961912 1101 88-89 1.0906372717101505 1101 90-91 1.104993774737622 1101 92-93 1.1139630043770445 1101 94-95 1.0655261850146118 1101 96-97 1.0639596671430205 1101 98-99 1.1052494294087083 1101 100-101 1.1168303269737514 1101 102-103 1.15440189822597 1101 104-105 1.0608667344671048 1101 106-107 1.0538161171891076 1101 108-109 1.0434258428692225 1101 110-111 1.0569223192219397 1101 112-113 1.0611154297971268 1101 114-115 1.0378308531741922 1101 116-117 1.0538413834041052 1101 118-119 1.16318996810395 1101 120-121 1.1422045653818742 1101 122-123 1.1820581699530557 1101 124-125 1.012525881831511 1102 1 0.23108341224436657 1102 2 -0.5867015904450348 1102 3 -0.18313580632728232 1102 4 0.1434163932440029 1102 5 0.546937427173745 1102 6 0.31861793956493045 1102 7 0.4403531169445003 1102 8 0.27051529459039614 1102 9 0.480816942375192 1102 10-11 0.5704279595899848 1102 12-13 0.6441038858872332 1102 14-15 0.7774651409352913 1102 16-17 0.9452097800443937 1102 18-19 0.9057817806265831 1102 20-21 0.8554047204762014 1102 22-23 0.7889195532411506 1102 24-25 0.9072431473459872 1102 26-27 0.9225972858770248 1102 28-29 0.9032446946734112 1102 30-31 0.7281233477813203 1102 32-33 0.7673491482711654 1102 34-35 0.6521272113203409 1102 36-37 0.6206327878025135 1102 38-39 0.5009746454106576 1102 40-41 0.5288503839161933 1102 42-43 0.4872785104231028 1102 44-45 0.29286958128950147 1102 46-47 0.3237240614578951 1102 48-49 0.3900254100904874 1102 50-51 0.3211680668889372 1102 52-53 0.48447305064169655 1102 54-55 0.4491323747114109 1102 56-57 0.3079596938477138 1102 58-59 0.5209588553619113 1102 60-61 0.3840796739363661 1102 62-63 0.5056564741600766 1102 64-65 0.5375867564348766 1102 66-67 0.5323599071698233 1102 68-69 0.29772360347522664 1102 70-71 0.23161382390741636 1102 72-73 0.31974605999256056 1102 74-75 0.2808058670499385 1102 76-77 0.3187351141335917 1102 78-79 0.37032379435559903 1102 80-81 0.31211916804775797 1102 82-83 0.28855707070636427 1102 84-85 0.40977106452625023 1102 86-87 0.30309464661605645 1102 88-89 0.4239998750724858 1102 90-91 0.39048060860808675 1102 92-93 0.5844019489513741 1102 94-95 0.30782262553479 1102 96-97 0.2412639309493123 1102 98-99 0.3899854214378493 1102 100-101 0.23877275124332087 1102 102-103 0.22276465223621145 1102 104-105 0.24322349386683229 1102 106-107 0.4127680094761246 1102 108-109 0.4113706914391244 1102 110-111 0.3614580155830751 1102 112-113 0.35789723761002534 1102 114-115 0.5025514203052097 1102 116-117 0.5057565164253006 1102 118-119 0.38008477361026394 1102 120-121 0.4334090528618546 1102 122-123 0.4080900651462649 1102 124-125 0.2402990949324355 1103 1 0.2781143090621967 1103 2 -1.0214970795729101 1103 3 -0.03858478180466918 1103 4 0.2967131832595413 1103 5 0.5489215016010007 1103 6 0.4983231906480228 1103 7 0.5582309777200933 1103 8 0.6413878458606774 1103 9 0.7584578620615687 1103 10-11 0.9302867915723922 1103 12-13 0.8277001651866343 1103 14-15 0.8604316338804807 1103 16-17 0.8678533191652065 1103 18-19 0.9670435736000229 1103 20-21 0.9445797237137157 1103 22-23 0.8494070126987765 1103 24-25 0.6539776378659852 1103 26-27 0.7554631127059181 1103 28-29 0.9690961083586345 1103 30-31 0.819537930673178 1103 32-33 0.8269101943378985 1103 34-35 0.8742366727763979 1103 36-37 0.8955631722854562 1103 38-39 0.9761160930308037 1103 40-41 1.068206420608874 1103 42-43 0.9814373003915549 1103 44-45 1.0706115349902632 1103 46-47 1.0858851629300759 1103 48-49 0.9806985403587873 1103 50-51 1.0075588081447435 1103 52-53 0.856496387153669 1103 54-55 0.9654994711566331 1103 56-57 0.9731910980706004 1103 58-59 0.9474757635904751 1103 60-61 0.8776444004556403 1103 62-63 0.8458986118098863 1103 64-65 0.8872948200989086 1103 66-67 0.9693072782436154 1103 68-69 0.8987814392631748 1103 70-71 0.9990162315797093 1103 72-73 0.9290874433041161 1103 74-75 0.8038030850045459 1103 76-77 0.7833806130475587 1103 78-79 0.8650009987274068 1103 80-81 0.8088075793313436 1103 82-83 0.8239655608378662 1103 84-85 0.7491698417801409 1103 86-87 0.7550735060878146 1103 88-89 0.7749660582474753 1103 90-91 0.9147601813606698 1103 92-93 0.7465963084113554 1103 94-95 0.6221345236535072 1103 96-97 0.7067499850362147 1103 98-99 0.7120969891104956 1103 100-101 0.7381927598962825 1103 102-103 0.823610808657655 1103 104-105 0.826642685418129 1103 106-107 0.7751778928377142 1103 108-109 0.8218618666675113 1103 110-111 0.8298948423295585 1103 112-113 0.8489533130055911 1103 114-115 0.8595170556799374 1103 116-117 0.8643684957759206 1103 118-119 0.818996352614171 1103 120-121 0.7746727515474277 1103 122-123 0.8350314817893896 1103 124-125 0.6917948582179712 1104 1 0.15821960672505853 1104 2 -0.826151512311224 1104 3 0.03742103803338637 1104 4 0.2707755107347012 1104 5 0.48750840811553786 1104 6 0.5188177043579927 1104 7 0.5778585540910868 1104 8 0.6836276783965616 1104 9 0.6446834785503412 1104 10-11 0.839358291618403 1104 12-13 0.6388983214711388 1104 14-15 0.8008207061568839 1104 16-17 0.972449380998107 1104 18-19 0.7705025529430429 1104 20-21 0.8458408803202673 1104 22-23 0.7777754164989261 1104 24-25 0.7806532854092367 1104 26-27 0.6443125445241051 1104 28-29 0.8629710450995276 1104 30-31 0.8384581551855064 1104 32-33 0.8527400388355062 1104 34-35 0.7540414896700227 1104 36-37 0.8008923209328671 1104 38-39 0.823893829274823 1104 40-41 0.7726303889103079 1104 42-43 0.7378307835531288 1104 44-45 0.838089020498181 1104 46-47 0.8937852192881905 1104 48-49 0.8588564638497758 1104 50-51 0.894890740192956 1104 52-53 0.7195979237749413 1104 54-55 1.0363532678533574 1104 56-57 0.9853929176326091 1104 58-59 0.9099796453994529 1104 60-61 0.8463483938306844 1104 62-63 0.8746735991580579 1104 64-65 0.8469261632623244 1104 66-67 0.8731728411346715 1104 68-69 0.80827921944352 1104 70-71 0.9186038396952796 1104 72-73 0.7782324389334825 1104 74-75 0.8898397867896044 1104 76-77 0.9597520417832968 1104 78-79 0.8338969259909348 1104 80-81 0.7919871259360534 1104 82-83 0.8374390322677492 1104 84-85 0.8749275133848506 1104 86-87 0.8446606253829927 1104 88-89 0.8174826493865979 1104 90-91 0.8164835607773071 1104 92-93 0.7922701041879492 1104 94-95 0.7761819564838319 1104 96-97 0.7795590632934868 1104 98-99 0.780222017864638 1104 100-101 0.8224625197416984 1104 102-103 0.8952999131387003 1104 104-105 0.8363321119455449 1104 106-107 0.7967966934450033 1104 108-109 0.7241933961577587 1104 110-111 0.6798542989913194 1104 112-113 0.6722972182779507 1104 114-115 0.6872452485397105 1104 116-117 0.62905035996436 1104 118-119 0.6890758003046393 1104 120-121 0.7142049504340662 1104 122-123 0.6781445775751784 1104 124-125 0.6943395996607364 1105 1 0.6149153274688679 1105 2 0.42025172026791324 1105 3 0.5997134545751841 1105 4 0.6472394788105547 1105 5 0.7317277573866008 1105 6 0.6545669287945941 1105 7 0.735130277239179 1105 8 0.8191973190042035 1105 9 0.8757630143498432 1105 10-11 0.9740638057372379 1105 12-13 0.914278975991877 1105 14-15 1.020428030227933 1105 16-17 1.1839904858582102 1105 18-19 1.1933988591687594 1105 20-21 1.1802538378113852 1105 22-23 1.0309661454091525 1105 24-25 1.1529717284402565 1105 26-27 1.0876136025063303 1105 28-29 1.2438668980647805 1105 30-31 1.1750425760864687 1105 32-33 1.2058565731168827 1105 34-35 1.1485123791840195 1105 36-37 1.2296131644537311 1105 38-39 1.209698263011724 1105 40-41 1.2146311757086679 1105 42-43 1.2165507581199861 1105 44-45 1.1635843155071868 1105 46-47 1.180374546707256 1105 48-49 1.1636444531147703 1105 50-51 1.2053923215137061 1105 52-53 1.090008486112822 1105 54-55 1.114629435130695 1105 56-57 1.1832627865908307 1105 58-59 0.9552708450545424 1105 60-61 1.1615974044519355 1105 62-63 1.2547656696630582 1105 64-65 1.1614926061192108 1105 66-67 1.171420371549818 1105 68-69 1.1725924455906451 1105 70-71 1.1718676397382524 1105 72-73 1.0821221579611553 1105 74-75 1.2422539165786795 1105 76-77 1.1807628143553863 1105 78-79 1.1833630195809661 1105 80-81 1.0288405978198583 1105 82-83 1.0989668043890397 1105 84-85 0.9298944281803081 1105 86-87 1.1010756125400256 1105 88-89 1.0713203101694262 1105 90-91 1.0272065322699966 1105 92-93 0.786118260881949 1105 94-95 0.9151917333575099 1105 96-97 0.9800551482606856 1105 98-99 0.9977588176281422 1105 100-101 1.0041534305178494 1105 102-103 0.5756386349231448 1105 104-105 0.5595402848881079 1105 106-107 0.9612867912312915 1105 108-109 0.9492894814058346 1105 110-111 0.8546763477426254 1105 112-113 0.6880749654716674 1105 114-115 0.8422350457907939 1105 116-117 0.5800658863293044 1105 118-119 0.4738174593994131 1105 120-121 0.5027483711004983 1105 122-123 0.5545197631275833 1105 124-125 0.392571927769346 1106 1 0.3289747719187588 1106 2 0.277863267808911 1106 3 0.4056532398031898 1106 4 0.3359036560300339 1106 5 0.45849562511953934 1106 6 0.5662035038943785 1106 7 0.5078607544313698 1106 8 0.4416130216076013 1106 9 0.4915844674421308 1106 10-11 0.5862149233318945 1106 12-13 0.5268179628016583 1106 14-15 0.5809912955958083 1106 16-17 0.8064728608713025 1106 18-19 0.7851994595729366 1106 20-21 0.7144846823681235 1106 22-23 0.7349682868358229 1106 24-25 0.7174458830160475 1106 26-27 0.6352572508137087 1106 28-29 0.7458266628345456 1106 30-31 0.7128294114536686 1106 32-33 0.6624998618833047 1106 34-35 0.6229513121572197 1106 36-37 0.7418061769100817 1106 38-39 0.7287580178257613 1106 40-41 0.8999481916252776 1106 42-43 0.8840746660465442 1106 44-45 0.8390694507838319 1106 46-47 0.6900358990111428 1106 48-49 0.7161475786795037 1106 50-51 0.8446169940365493 1106 52-53 0.803025244573341 1106 54-55 0.8495690791902959 1106 56-57 0.7455447901642316 1106 58-59 0.7965112854518139 1106 60-61 0.932140029314187 1106 62-63 0.8657475053647019 1106 64-65 0.7896384820846265 1106 66-67 0.662286079474967 1106 68-69 0.7355932731710624 1106 70-71 0.7250282902408784 1106 72-73 0.881809953978486 1106 74-75 0.9395452284974048 1106 76-77 0.982040560351642 1106 78-79 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-1.7373945026302948 2216 108-109 -1.7158484698709486 2216 110-111 -1.678148117591725 2216 112-113 -1.6911864425181413 2216 114-115 -1.7937202212275523 2216 116-117 -1.8733845534797915 2216 118-119 -1.9292888977073162 2216 120-121 -1.8824112576393048 2216 122-123 -1.87514583535269 2216 124-125 -1.8530702390074971 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 73967.0 3 23843.0 4 11474.0 5 8945.0 6 9192.0 7 11389.0 8 12973.0 9 14130.0 10 15095.0 11 16501.0 12 18447.0 13 19845.0 14 21485.0 15 18148.0 16 13012.0 17 11170.0 18 10248.0 19 9608.0 20 9358.0 21 9832.0 22 11817.0 23 15693.0 24 21441.0 25 28408.0 26 34522.0 27 39477.0 28 45660.0 29 54331.0 30 62881.0 31 74637.0 32 90833.0 33 112658.0 34 145582.0 35 199194.0 36 318054.0 37 488273.0 38 190146.0 39 785.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.650838228594196 19.721624911136985 13.64539750105095 24.98213935921787 2 15.533481760575441 20.563263333026615 38.77195755338956 25.13129735300838 3 19.616339954968073 24.337961174701526 28.75505817283707 27.29064069749333 4 13.333688242504449 16.979104062595418 36.10306210353199 33.58414559136814 5 13.439204291166698 38.22175692630091 34.09482267340671 14.244216109125682 6 32.57737301516423 37.111683128417496 16.55249539494784 13.75844846147044 7 28.61628330544132 32.09948562549383 20.490336340743635 18.793894728321217 8 25.952133121342474 36.90545847129017 18.640604226736365 18.501804180630995 9 25.75900489300242 16.476422345033658 19.563254880464154 38.20131788149977 10-11 25.08454961674802 27.086514627754987 26.786986908290245 21.041948847206747 12-13 25.844410316856397 23.90996124458195 27.690049930846538 22.555578507715115 14-15 22.59909476381915 25.525614444181688 25.516419092453983 26.358871699545183 16-17 22.415327669363368 28.701810513045455 26.160683197852613 22.72217861973856 18-19 22.297913507363898 27.318306820486495 28.42285846015638 21.96092121199323 20-21 22.859824231687355 26.419650641284413 28.751905945979477 21.968619181048755 22-23 22.59340494947784 26.23331052704525 28.580275567425577 22.59300895605133 24-25 22.442053730355724 26.417476223618092 28.699824113285473 22.44064593274071 26-27 22.253672811996548 26.685793650304834 28.574068191956727 22.486465345741895 28-29 22.247336366251375 26.708239256165516 28.628634951390925 22.415789426192184 30-31 22.41389777805543 26.51465825272959 28.63438352102502 22.437060448189968 32-33 22.281178253429594 26.497899558486026 28.590069972188424 22.630852215895956 34-35 22.543041212395305 26.54340811964872 28.560518139912205 22.353032528043766 36-37 22.565466596951186 26.358720458450403 28.44184802919955 22.633964915398856 38-39 22.41033429034242 26.571322106733934 28.52835876314421 22.489984839779435 40-41 22.57557453540479 26.451505331593527 28.457374967774633 22.515545165227046 42-43 22.438846800235815 26.407425494285036 28.312413659601056 22.841314045878097 44-45 22.58274550450627 26.634672999409602 28.172933859028426 22.609647637055698 46-47 22.743645384345502 26.802064624552223 27.761831281943554 22.692458709158718 48-49 22.771577465241062 26.82723223899534 27.643712837627533 22.757477458136066 50-51 22.722415404189274 27.05922102695768 27.522168510596362 22.69619505825668 52-53 22.862125459514345 27.050107080700307 27.34442502855192 22.743342431233426 54-55 22.770444520895676 26.963151777300492 27.420092087561493 22.84631161424234 56-57 22.768563409032915 27.14478975108006 27.366939138238994 22.71970770164803 58-59 22.74853567051201 26.88149951562963 27.501920323934232 22.868044489924127 60-61 22.78823996262297 26.9897239572839 27.52402714585751 22.698008934235613 62-63 22.68863828136067 27.06127967043458 27.61689779477302 22.633184253431725 64-65 22.593638997676237 26.886330100948598 27.630774320534897 22.889256580840268 66-67 22.565038050129914 27.049995292676726 27.483794929640915 22.90117172755245 68-69 22.63733000445439 26.88545613523754 27.633640008138887 22.84357385216918 70-71 22.623879591069986 26.749870438625745 27.53471760899653 23.09153236130774 72-73 22.678929039024666 26.929346104572588 27.3820909941998 23.00963386220295 74-75 22.541567622811083 27.10141254905998 27.32420348062436 23.03281634750458 76-77 22.69233265032957 26.91017235671494 27.44966834173065 22.947826651224844 78-79 22.732428024708792 26.91205642495041 27.376963067473163 22.978552482867634 80-81 22.627106694767296 27.044596806497427 27.35816621180222 22.970130286933056 82-83 22.77949524274181 26.99200150634411 27.318104769224476 22.910398481689604 84-85 22.739082032165104 26.956382680829993 27.31785679483567 22.986678492169236 86-87 22.698411755687143 26.998811505890007 27.421240543244974 22.881536195177876 88-89 22.736239438218362 26.9189161366162 27.338901759483058 23.005942665682383 90-91 22.651749584479735 27.066932857732372 27.380849729042957 22.90046782874494 92-93 22.643677275941982 26.901590786351992 27.457282607380552 22.997449330325473 94-95 22.552021670544892 26.85491566675334 27.44668045493362 23.146382207768156 96-97 22.705461725596702 26.85285174172342 27.36104918067407 23.08063735200581 98-99 22.53511987159027 26.900227954984445 27.25341190077678 23.311240272648504 100-101 22.436501505028936 26.835664566401206 27.476126747984864 23.251707180584994 102-103 22.459084223050624 26.68975895200003 27.50672168516931 23.344435139780035 104-105 22.443923900469393 26.698582674948344 27.461258542763805 23.396234881818458 106-107 22.384824718610254 26.770098631321854 27.395582719357737 23.44949393071016 108-109 22.392401123201278 26.696554174223785 27.24667202676677 23.664372675808167 110-111 22.324400314731793 26.75308226206547 27.36974364580751 23.552773777395227 112-113 22.37673110329328 26.79198076030072 27.381309836720803 23.4499782996852 114-115 22.275542779738807 26.84564688460785 27.2939003173937 23.58491001825965 116-117 22.12449629315315 26.78747052109228 27.433262348585487 23.654770837169085 118-119 22.063886128235957 26.780203192854596 27.311304422807964 23.844606256101482 120-121 22.039772844768603 26.83534061625118 27.416032245588823 23.708854293391386 122-123 22.047417471901117 26.759336818820927 27.354793835931023 23.83845187334693 124-125 21.997202832890604 26.734789139002224 27.361182113264594 23.90682591484258 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 84.0 1 74.5 2 138.5 3 270.0 4 233.5 5 167.0 6 455.0 7 1009.0 8 1593.5 9 1917.0 10 1666.5 11 1255.0 12 1192.5 13 1356.5 14 1522.0 15 1648.5 16 1789.5 17 2051.0 18 2293.0 19 2489.0 20 2809.5 21 3342.5 22 4089.0 23 5110.0 24 6433.5 25 8098.5 26 10387.5 27 13504.5 28 16551.0 29 20142.5 30 24712.0 31 28877.0 32 33633.5 33 38341.0 34 42767.5 35 47321.0 36 52667.5 37 58141.5 38 62107.0 39 65382.5 40 68160.0 41 71380.0 42 73791.5 43 76468.5 44 80422.0 45 83302.5 46 84896.0 47 86122.5 48 87382.5 49 87655.0 50 85899.5 51 83330.0 52 81796.5 53 80497.5 54 79069.5 55 74750.0 56 67760.5 57 61689.0 58 56691.5 59 50534.5 60 42396.0 61 34484.0 62 27286.5 63 20777.0 64 15445.0 65 11289.0 66 8291.5 67 6217.5 68 4645.0 69 3506.5 70 2629.0 71 1940.0 72 1427.0 73 1182.0 74 859.5 75 540.0 76 359.5 77 219.5 78 141.5 79 75.0 80 40.0 81 27.5 82 24.5 83 22.5 84 16.0 85 9.5 86 6.5 87 5.0 88 3.0 89 1.0 90 1.0 91 1.5 92 1.0 93 0.5 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05714778443450969 2 0.07404135581468808 3 0.0 4 0.007566912180704902 5 0.029255794187027673 6 3.079557282845018E-4 7 8.798735093842908E-5 8 0.0 9 5.279241056305745E-4 10-11 0.057675708540140265 12-13 0.009260668686269662 14-15 0.007324946965624222 16-17 0.05573998681949483 18-19 2.1996837734607273E-4 20-21 0.010228529546592383 22-23 0.012714172210603005 24-25 0.0 26-27 0.0 28-29 0.05299038210266892 30-31 0.0 32-33 0.0034974971998025565 34-35 0.0 36-37 2.4196521508067998E-4 38-39 0.0 40-41 0.0 42-43 0.003255531984721876 44-45 0.0 46-47 6.599051320382182E-5 48-49 2.4196521508067998E-4 50-51 4.3993675469214546E-4 52-53 8.798735093842908E-5 54-55 0.0 56-57 0.0014957849659532946 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.038988075074327E-4 66-67 0.0 68-69 7.2589564524204E-4 70-71 0.0 72-73 0.0013198102640764363 74-75 0.0031235509583142328 76-77 2.8595889054989457E-4 78-79 7.478924829766473E-4 80-81 0.0014297944527494728 82-83 4.399367546921454E-5 84-85 2.199683773460727E-5 86-87 4.179399169575382E-4 88-89 0.0 90-91 0.0 92-93 0.005345231569509567 94-95 0.004707323275205956 96-97 0.005213250543101924 98-99 7.698893207112545E-4 100-101 3.5194940375371633E-4 102-103 2.199683773460727E-5 104-105 1.9797153961146545E-4 106-107 0.004157402331840775 108-109 0.003805452928087058 110-111 3.299525660191091E-4 112-113 0.002001712233849262 114-115 4.179399169575382E-4 116-117 0.0013418071018110437 118-119 0.0018037406942377964 120-121 0.010866437840895993 122-123 4.3993675469214546E-4 124-125 0.0017597470187685818 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2273054.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.39543675394391 #Duplication Level Percentage of deduplicated Percentage of total 1 80.69785142132527 51.96573387381114 2 11.52271463484614 14.840204830039571 3 3.450656727503047 6.666196411664806 4 1.4762436135359516 3.8025340899547198 5 0.7723990884450636 2.486948832438401 6 0.45752319890211773 1.7677443731018052 7 0.3012266715877106 1.3578336155179218 8 0.2274892282142614 1.1719414566139987 9 0.15522860777836656 0.8996412595135111 >10 0.8714219589136459 10.075756785039026 >50 0.048654027919400024 2.10808346540353 >100 0.017837333069565076 2.0394020272629585 >500 4.1099343243285354E-4 0.15959059255462915 >1k 2.739956216218286E-4 0.43038292193342415 >5k 6.849890540545714E-5 0.22800546515054518 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5169 0.22740330850036997 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4394 0.1933082100117287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2743 0.1206746518120555 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 4.399367546921455E-5 3 0.0 0.0 0.0 0.0 4.399367546921455E-5 4 0.0 0.0 0.0 0.0 4.399367546921455E-5 5 0.0 0.0 0.0 0.0 4.399367546921455E-5 6 0.0 0.0 0.0 0.0 8.79873509384291E-5 7 0.0 0.0 0.0 0.0 8.79873509384291E-5 8 0.0 0.0 0.0 0.0 8.79873509384291E-5 9 0.0 0.0 0.0 0.0 8.79873509384291E-5 10-11 0.0 0.0 0.0 2.1996837734607274E-5 8.79873509384291E-5 12-13 0.0 0.0 0.0 4.399367546921455E-5 8.79873509384291E-5 14-15 0.0 0.0 0.0 8.79873509384291E-5 8.79873509384291E-5 16-17 0.0 0.0 0.0 8.79873509384291E-5 8.79873509384291E-5 18-19 0.0 0.0 0.0 8.79873509384291E-5 8.79873509384291E-5 20-21 2.1996837734607274E-5 0.0 0.0 8.79873509384291E-5 1.539778641422509E-4 22-23 4.399367546921455E-5 0.0 0.0 1.9797153961146545E-4 1.9797153961146545E-4 24-25 4.399367546921455E-5 0.0 0.0 2.6396205281528726E-4 2.4196521508068E-4 26-27 4.399367546921455E-5 0.0 0.0 4.3993675469214546E-4 2.6396205281528726E-4 28-29 4.399367546921455E-5 0.0 0.0 7.918861584458618E-4 2.6396205281528726E-4 30-31 4.399367546921455E-5 0.0 0.0 0.0018257375319724036 2.6396205281528726E-4 32-33 4.399367546921455E-5 0.0 0.0 0.004311380195983025 2.6396205281528726E-4 34-35 4.399367546921455E-5 0.0 0.0 0.007566912180704901 2.6396205281528726E-4 36-37 4.399367546921455E-5 0.0 0.0 0.013198102640764365 2.6396205281528726E-4 38-39 4.399367546921455E-5 0.0 0.0 0.0229866954326646 2.6396205281528726E-4 40-41 4.399367546921455E-5 0.0 0.0 0.036294782262102004 2.6396205281528726E-4 42-43 4.399367546921455E-5 0.0 0.0 0.04793110942370925 2.6396205281528726E-4 44-45 4.399367546921455E-5 0.0 0.0 0.061107215226739 3.079557282845018E-4 46-47 4.399367546921455E-5 0.0 0.0 0.0768569510447178 3.079557282845018E-4 48-49 4.399367546921455E-5 0.0 0.0 0.0958842156851531 3.079557282845018E-4 50-51 4.399367546921455E-5 0.0 0.0 0.1182110059857795 3.079557282845018E-4 52-53 4.399367546921455E-5 0.0 0.0 0.13937196388647166 3.079557282845018E-4 54-55 4.399367546921455E-5 0.0 0.0 0.1624026529946055 3.079557282845018E-4 56-57 4.399367546921455E-5 0.0 0.0 0.19073457999677967 3.079557282845018E-4 58-59 4.399367546921455E-5 0.0 0.0 0.22214606428179884 3.519494037537164E-4 60-61 4.399367546921455E-5 0.0 0.0 0.2591227485136737 3.519494037537164E-4 62-63 4.399367546921455E-5 0.0 0.0 0.2985630785718245 3.519494037537164E-4 64-65 4.399367546921455E-5 0.0 0.0 0.3385533295733405 3.519494037537164E-4 66-67 4.399367546921455E-5 0.0 0.0 0.385406593948054 3.519494037537164E-4 68-69 4.399367546921455E-5 0.0 0.0 0.4404206851223068 3.519494037537164E-4 70-71 4.399367546921455E-5 0.0 0.0 0.5026497390735107 3.519494037537164E-4 72-73 4.399367546921455E-5 0.0 0.0 0.5705979708357126 3.959430792229309E-4 74-75 4.399367546921455E-5 0.0 0.0 0.6394480729450334 3.959430792229309E-4 76-77 4.399367546921455E-5 0.0 0.0 0.7156011251822438 3.959430792229309E-4 78-79 4.399367546921455E-5 0.0 0.0 0.8067780175921909 3.959430792229309E-4 80-81 4.399367546921455E-5 0.0 0.0 0.9098112055410914 3.959430792229309E-4 82-83 4.399367546921455E-5 4.399367546921455E-5 0.0 1.0224570115800153 3.959430792229309E-4 84-85 4.399367546921455E-5 4.399367546921455E-5 0.0 1.1332110895737628 3.959430792229309E-4 86-87 4.399367546921455E-5 4.399367546921455E-5 0.0 1.2523459627443958 3.959430792229309E-4 88-89 4.399367546921455E-5 4.399367546921455E-5 0.0 1.392091872872356 3.959430792229309E-4 90-91 4.399367546921455E-5 4.399367546921455E-5 0.0 1.5553743993763458 3.959430792229309E-4 92-93 4.399367546921455E-5 4.399367546921455E-5 0.0 1.7317010506569575 3.959430792229309E-4 94-95 4.399367546921455E-5 4.399367546921455E-5 0.0 1.9150666900126438 3.959430792229309E-4 96-97 4.399367546921455E-5 4.399367546921455E-5 0.0 2.0994001902286525 3.959430792229309E-4 98-99 4.399367546921455E-5 4.399367546921455E-5 0.0 2.301639116360632 3.959430792229309E-4 100-101 4.399367546921455E-5 4.399367546921455E-5 0.0 2.542328514852705 3.959430792229309E-4 102-103 8.79873509384291E-5 4.399367546921455E-5 0.0 2.808182295713168 3.959430792229309E-4 104-105 8.79873509384291E-5 8.79873509384291E-5 0.0 3.0759058077810733 3.959430792229309E-4 106-107 8.79873509384291E-5 8.79873509384291E-5 0.0 3.346620889780885 3.959430792229309E-4 108-109 8.79873509384291E-5 8.79873509384291E-5 0.0 3.6337016190552447 3.959430792229309E-4 110-111 8.79873509384291E-5 8.79873509384291E-5 0.0 3.963984137640373 3.959430792229309E-4 112-113 8.79873509384291E-5 8.79873509384291E-5 0.0 4.323324478872918 3.959430792229309E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4460 0.0 56.497208 1 GTATCAA 6905 0.0 43.65242 1 ATCAACG 8470 0.0 34.352573 3 TCAACGC 8540 0.0 33.79378 4 TATCAAC 8865 0.0 33.112984 2 CAACGCA 8740 0.0 32.57614 5 AACGCAG 9055 0.0 31.433208 6 GTACATG 8090 0.0 27.686037 1 TACATGG 8140 0.0 27.284414 2 ACGCAGA 10870 0.0 25.910803 7 CGCAGAG 11040 0.0 25.56575 8 ACATGGG 8305 0.0 24.717999 3 GTGGTAT 2510 0.0 23.25808 1 GAGTACT 7355 0.0 23.19943 12-13 GTACTTT 7635 0.0 22.19262 14-15 GCAGAGT 12620 0.0 21.75302 9 CAGAGTA 12105 0.0 21.312786 10-11 TATAGGG 770 0.0 20.878689 2 TGGTATC 2500 0.0 20.720938 2 TACCGTG 345 1.2732926E-9 20.711494 7 >>END_MODULE