FastQCFastQC Report
Thu 26 May 2016
SRR1364636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463805
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31410.12748573852232625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64900.042.8698161
ATCAACG74250.037.6303443
TCAACGC74600.037.3739364
CAACGCA76400.036.337445
AACGCAG77050.035.6874586
GGTATCA41450.034.3520661
TATCAAC82800.033.672652
GTACATG97700.033.2339061
TACATGG101850.030.3576452
ACATGGG102700.029.7003293
ACGCAGA90350.029.5052287
CGCAGAG90450.027.889188
GCAGAGT102600.023.3687869
CATGGGA64500.023.3680554
CATGGGG45200.022.6700134
TACCTGG22150.021.5168172
GTACGCT2055.883414E-520.3425753
ATAGCGC2055.9445432E-520.315278
GTATAGA11150.020.3043081
GTACCTG24750.019.9793971
Produced by FastQC (version 0.11.5)