FastQCFastQC Report
Thu 26 May 2016
SRR1364636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2463805
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74550.3025807643056167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63530.25785319860946787No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40660.1650292941202733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58050.050.999191
GTATCAA95050.045.9994931
ATCAACG116400.037.061813
TCAACGC117950.036.6281934
CAACGCA119650.035.547045
TATCAAC122100.035.306572
AACGCAG123000.034.6637736
GTACATG106300.030.3160821
ACGCAGA144400.029.362267
CGCAGAG146150.028.9699348
TACATGG111100.028.2977872
ACATGGG108600.027.6696133
GAGTACT86050.026.02091212-13
GCAGAGT158300.025.9945749
CATGGGG45650.023.9857834
GTACTTT93550.023.9029614-15
AGTACTT93500.022.70562612-13
CAGAGTA156050.022.01087610-11
ATACCGT3256.039045E-1021.986996
AGAGTAC147850.021.94210610-11
Produced by FastQC (version 0.11.5)