##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2463805 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.737812448631285 31.0 30.0 33.0 26.0 34.0 2 29.776142186577264 31.0 30.0 34.0 26.0 34.0 3 29.797631306048977 31.0 30.0 34.0 26.0 34.0 4 33.487777644740554 35.0 33.0 37.0 28.0 37.0 5 32.93866925345147 35.0 33.0 37.0 26.0 37.0 6 32.985013018481574 35.0 33.0 37.0 26.0 37.0 7 32.79940539125458 35.0 33.0 37.0 25.0 37.0 8 32.7402736823734 35.0 33.0 37.0 25.0 37.0 9 34.026941255497086 38.0 34.0 39.0 25.0 39.0 10-11 33.84393793339976 38.0 33.0 39.0 24.0 39.0 12-13 33.72107370510247 37.5 33.0 39.0 23.0 39.0 14-15 34.72818161339879 39.0 33.0 41.0 23.5 41.0 16-17 34.6554958691942 39.0 33.0 41.0 23.0 41.0 18-19 34.69604493862136 39.0 33.5 41.0 23.0 41.0 20-21 34.71659141044036 39.0 34.0 41.0 23.0 41.0 22-23 34.7104856106713 39.0 34.0 41.0 23.0 41.0 24-25 34.713348458989245 39.0 34.0 41.0 23.5 41.0 26-27 34.62777086660673 39.0 34.0 41.0 23.0 41.0 28-29 34.397899996144176 39.0 33.0 41.0 21.5 41.0 30-31 34.33997597212442 38.5 33.0 41.0 22.0 41.0 32-33 34.1727529167284 38.0 33.0 41.0 20.0 41.0 34-35 34.04793277065352 38.0 33.0 41.0 19.0 41.0 36-37 33.89389338847839 38.0 33.0 41.0 18.0 41.0 38-39 33.76401297992333 38.0 33.0 40.5 16.5 41.0 40-41 33.605269288762706 38.0 33.0 40.0 15.5 41.0 42-43 33.43971661718358 38.0 33.0 40.0 15.0 41.0 44-45 33.248536714553296 38.0 32.0 40.0 10.5 41.0 46-47 33.010740500973085 38.0 32.0 40.0 8.5 41.0 48-49 32.765601579670474 38.0 31.5 40.0 6.5 41.0 50-51 32.578041890490525 38.0 31.0 40.0 2.0 41.0 52-53 32.414168734944525 38.0 31.0 40.0 2.0 41.0 54-55 32.20328759784155 37.5 31.0 40.0 2.0 41.0 56-57 31.990309500954822 37.0 31.0 40.0 2.0 41.0 58-59 31.808111843266815 37.0 30.5 40.0 2.0 41.0 60-61 31.59897820647332 36.5 30.0 40.0 2.0 41.0 62-63 31.338304370678685 36.0 30.0 40.0 2.0 41.0 64-65 31.072743175697752 36.0 29.5 39.0 2.0 41.0 66-67 30.79859424751553 35.0 29.0 39.0 2.0 41.0 68-69 30.48556156026958 35.0 29.0 39.0 2.0 40.5 70-71 30.22801865407368 35.0 29.0 38.0 2.0 40.0 72-73 30.07069005055189 35.0 29.0 37.5 2.0 40.0 74-75 29.785273185174965 35.0 29.0 37.0 2.0 39.0 76-77 29.48356688130757 35.0 29.0 36.5 2.0 39.0 78-79 29.170622269213677 34.0 29.0 36.0 2.0 39.0 80-81 28.88841933513407 34.0 29.0 36.0 2.0 37.5 82-83 28.633350447783002 34.0 28.5 35.0 2.0 37.0 84-85 28.602454536783554 34.0 29.0 35.0 2.0 37.0 86-87 28.44839526667086 34.0 29.0 35.0 2.0 36.0 88-89 28.302556208790875 34.0 29.0 35.0 2.0 36.0 90-91 28.142751759981003 34.0 29.0 35.0 2.0 36.0 92-93 28.0090490115898 34.0 28.0 35.0 2.0 35.5 94-95 27.92404573413886 34.0 28.0 35.0 2.0 35.0 96-97 27.84177664222615 34.0 28.0 35.0 2.0 35.0 98-99 27.749953019820968 34.0 28.0 35.0 2.0 35.0 100-101 27.652734084069152 34.0 27.0 35.0 2.0 35.0 102-103 26.464663193718657 32.5 24.5 34.5 2.0 35.0 104-105 27.399073384460216 34.0 27.0 35.0 2.0 35.0 106-107 27.570271997986854 34.0 27.0 35.0 2.0 35.0 108-109 27.579675948380654 34.0 27.0 35.0 2.0 35.0 110-111 27.52438646727318 34.0 27.0 35.0 2.0 35.0 112-113 27.48001972558705 34.0 27.0 35.0 2.0 35.0 114-115 27.405159296291714 34.0 27.0 35.0 2.0 35.0 116-117 27.288463778586376 34.0 27.0 35.0 2.0 35.0 118-119 27.18858675909822 34.0 27.0 35.0 2.0 35.0 120-121 27.054653067105555 34.0 27.0 35.0 2.0 35.0 122-123 26.874795894967335 34.0 27.0 35.0 2.0 35.0 124-125 26.072713343791413 33.0 23.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6724908906107814 1101 2 0.6512663957810609 1101 3 0.5489203189022369 1101 4 0.6725874212502134 1101 5 0.8626807583852951 1101 6 0.908088240339481 1101 7 0.8524793808194104 1101 8 1.0273099720947556 1101 9 1.015130757032189 1101 10-11 1.2343555705582148 1101 12-13 1.090565169083014 1101 14-15 1.2392875597610882 1101 16-17 1.2961130104693694 1101 18-19 1.2782952962298566 1101 20-21 1.282606590314657 1101 22-23 1.185799590624356 1101 24-25 1.1119518660254997 1101 26-27 1.2524228764998213 1101 28-29 1.3716587050586355 1101 30-31 1.3312013377655703 1101 32-33 1.392208023315746 1101 34-35 1.3205653292128332 1101 36-37 1.3757750539216786 1101 38-39 1.3583361169687933 1101 40-41 1.4119446207279651 1101 42-43 1.390453637669303 1101 44-45 1.2651384575129327 1101 46-47 1.4039073691363484 1101 48-49 1.393460198462293 1101 50-51 1.327858970041639 1101 52-53 1.3457444927998026 1101 54-55 1.3993746240115001 1101 56-57 1.401428085107213 1101 58-59 1.4010082996837028 1101 60-61 1.2708178639476557 1101 62-63 1.3140384133631855 1101 64-65 1.3077911798477935 1101 66-67 1.29344985776153 1101 68-69 1.3799660624081937 1101 70-71 1.3736688474074121 1101 72-73 1.4058113291969434 1101 74-75 1.4069626972056497 1101 76-77 1.3521424143224579 1101 78-79 1.1734596266281478 1101 80-81 1.2150176054132338 1101 82-83 1.18264979473296 1101 84-85 1.2609009566762879 1101 86-87 1.269241708156816 1101 88-89 1.1941393897953674 1101 90-91 1.2900372367158113 1101 92-93 1.2180158950886657 1101 94-95 1.193284528520227 1101 96-97 1.129052074367614 1101 98-99 1.2329019126755654 1101 100-101 1.2856734097342652 1101 102-103 1.2617868281148255 1101 104-105 1.258898281533959 1101 106-107 1.2032000606513797 1101 108-109 1.2292898723253352 1101 110-111 1.2858318436951492 1101 112-113 1.2712720352598907 1101 114-115 1.181635568230103 1101 116-117 1.2529606417396408 1101 118-119 1.2872268110353176 1101 120-121 1.2845305970844478 1101 122-123 1.3385830042423557 1101 124-125 1.2022052286655445 1102 1 0.41063453131151206 1102 2 -0.5062622062518471 1102 3 0.10291731056092956 1102 4 0.3328077290734228 1102 5 0.6742074156319049 1102 6 0.6276091847763254 1102 7 0.6936160172507257 1102 8 0.6363760188607941 1102 9 0.9087149374111192 1102 10-11 0.9600190769471482 1102 12-13 1.0136206866544697 1102 14-15 1.0485705717488756 1102 16-17 1.2183049973420665 1102 18-19 1.1928055291484014 1102 20-21 1.1124708351146282 1102 22-23 1.0578960854509205 1102 24-25 1.0727610034823911 1102 26-27 1.0265585101507 1102 28-29 1.2680657883349866 1102 30-31 1.1939551855556587 1102 32-33 1.1192647867659247 1102 34-35 1.0164319746084018 1102 36-37 0.9417648437935995 1102 38-39 0.8296184604362864 1102 40-41 0.8373142060934455 1102 42-43 0.6747938537229103 1102 44-45 0.5704724137856303 1102 46-47 0.5838947584126757 1102 48-49 0.634873283227634 1102 50-51 0.6448462225751825 1102 52-53 0.6531663594907755 1102 54-55 0.6844554541921468 1102 56-57 0.5846078107100183 1102 58-59 0.782145543860743 1102 60-61 0.5151804146564345 1102 62-63 0.723101193495971 1102 64-65 0.5634069022295485 1102 66-67 0.5842305071378249 1102 68-69 0.5344426414075407 1102 70-71 0.3863945109897706 1102 72-73 0.39324048883654683 1102 74-75 0.42622618536220713 1102 76-77 0.6194692447765959 1102 78-79 0.5514078011119459 1102 80-81 0.5297334842895118 1102 82-83 0.5275698761708867 1102 84-85 0.6266035107276693 1102 86-87 0.5146898446565018 1102 88-89 0.6454321409083015 1102 90-91 0.705173447725123 1102 92-93 0.7729030822895417 1102 94-95 0.5151983197899597 1102 96-97 0.5460039257130127 1102 98-99 0.5673373169024352 1102 100-101 0.37737327451084113 1102 102-103 0.35193686533925117 1102 104-105 0.35302419125333273 1102 106-107 0.6377853501662436 1102 108-109 0.6864839103398275 1102 110-111 0.6561216624349875 1102 112-113 0.6665512184496478 1102 114-115 0.7201823722473044 1102 116-117 0.7020377798650479 1102 118-119 0.7100373311137176 1102 120-121 0.8015588724540343 1102 122-123 0.6896546118817177 1102 124-125 0.5731754187380176 1103 1 0.2871026295182375 1103 2 -1.0968502151797317 1103 3 -0.12613140797982325 1103 4 0.2546748883130121 1103 5 0.5040658009162868 1103 6 0.5440264864002771 1103 7 0.4969901417793139 1103 8 0.6425601179910956 1103 9 0.7800210253045989 1103 10-11 0.8498170450838387 1103 12-13 0.8118501178866708 1103 14-15 0.8488172430334089 1103 16-17 0.9971289794862912 1103 18-19 1.046370525684992 1103 20-21 1.084020900534469 1103 22-23 0.9373407864437624 1103 24-25 0.8621764579578794 1103 26-27 0.9154956301383876 1103 28-29 1.0364141297495948 1103 30-31 0.9768301951762268 1103 32-33 1.013224611286816 1103 34-35 1.052800018939955 1103 36-37 1.0683714781351625 1103 38-39 1.0998670558338048 1103 40-41 1.2219155298831765 1103 42-43 1.147719275103654 1103 44-45 1.189935971085717 1103 46-47 1.1012025395255804 1103 48-49 1.1304851511565204 1103 50-51 1.1690477156868582 1103 52-53 1.1871719779006824 1103 54-55 1.0683460106456266 1103 56-57 1.1538539432429218 1103 58-59 1.147947129329843 1103 60-61 1.020047266037949 1103 62-63 1.022726761109304 1103 64-65 1.0589167921702902 1103 66-67 1.0102549048904628 1103 68-69 0.9363328900029373 1103 70-71 0.9290320850681226 1103 72-73 0.929927551100846 1103 74-75 0.9359633515286063 1103 76-77 0.838129292494596 1103 78-79 0.8861738289731029 1103 80-81 0.8551687186464747 1103 82-83 0.8423205111281717 1103 84-85 0.8867374289913528 1103 86-87 0.9079312053892075 1103 88-89 0.7554613874256582 1103 90-91 0.827055999868584 1103 92-93 0.8832209457544842 1103 94-95 0.8101744607889572 1103 96-97 0.8650080048325393 1103 98-99 0.8473080703850826 1103 100-101 0.8336755141783563 1103 102-103 0.8679785801087441 1103 104-105 0.9109907886519331 1103 106-107 0.9333002428007404 1103 108-109 0.8848803545790354 1103 110-111 0.7903701039034701 1103 112-113 0.7808600770658245 1103 114-115 0.7913252953597514 1103 116-117 0.8014574673890991 1103 118-119 0.7614588657862313 1103 120-121 0.789340755006176 1103 122-123 0.818782219054814 1103 124-125 0.702971847584319 1104 1 0.2104190678382416 1104 2 -0.9506753517917552 1104 3 -0.1443476641504624 1104 4 0.09551682314401688 1104 5 0.4268780286821894 1104 6 0.3057465219502049 1104 7 0.36456579536114475 1104 8 0.4731484516324116 1104 9 0.5119270819908905 1104 10-11 0.6386172791501679 1104 12-13 0.5835969478717971 1104 14-15 0.512967916634075 1104 16-17 0.6745296992842569 1104 18-19 0.6329495494700126 1104 20-21 0.6883691665957343 1104 22-23 0.6370233449823388 1104 24-25 0.5508117016596472 1104 26-27 0.5253013374850752 1104 28-29 0.7303706426892447 1104 30-31 0.7227571330430536 1104 32-33 0.730061548009715 1104 34-35 0.7317490083189782 1104 36-37 0.7546763979278523 1104 38-39 0.8397837247003466 1104 40-41 0.8630322286679686 1104 42-43 0.8134012792746077 1104 44-45 0.8579062816195773 1104 46-47 0.8572319474081596 1104 48-49 0.8002793751738295 1104 50-51 0.8306014409492946 1104 52-53 0.5139921516025439 1104 54-55 0.8238980464067325 1104 56-57 0.8447273362367724 1104 58-59 0.7074901720161506 1104 60-61 0.7374027124798772 1104 62-63 0.7394477646027013 1104 64-65 0.620815897262549 1104 66-67 0.656371911657267 1104 68-69 0.5829851255123835 1104 70-71 0.6853171180221942 1104 72-73 0.6317642238391556 1104 74-75 0.5518088061540531 1104 76-77 0.6252860025427296 1104 78-79 0.6484796903108005 1104 80-81 0.5510562788129576 1104 82-83 0.5780743631032124 1104 84-85 0.6355257397540015 1104 86-87 0.655167946763509 1104 88-89 0.6358055353274175 1104 90-91 0.6382502661234355 1104 92-93 0.6631664769354622 1104 94-95 0.6651393508393824 1104 96-97 0.6826351354081126 1104 98-99 0.7277798108354361 1104 100-101 0.7304344434568897 1104 102-103 0.7084962538765929 1104 104-105 0.6205637495910139 1104 106-107 0.6523412308070426 1104 108-109 0.6071789123432616 1104 110-111 0.5417252247644129 1104 112-113 0.6083366924314859 1104 114-115 0.6630199883896744 1104 116-117 0.6292524041647916 1104 118-119 0.4779451429876431 1104 120-121 0.551411450077584 1104 122-123 0.5953903251414197 1104 124-125 0.4901340573112698 1105 1 0.47175501049134283 1105 2 0.313681784744837 1105 3 0.5234229619366637 1105 4 0.4425968914434435 1105 5 0.597877388749076 1105 6 0.4940955358533259 1105 7 0.5829752787394895 1105 8 0.6258625531448061 1105 9 0.664434997084264 1105 10-11 0.8030732127521532 1105 12-13 0.7948265627392246 1105 14-15 0.868903634633746 1105 16-17 1.0102290823351652 1105 18-19 0.9262403616635311 1105 20-21 0.9113401860757193 1105 22-23 0.8443153977141975 1105 24-25 0.8743152720190182 1105 26-27 0.8380932241421135 1105 28-29 0.942450081884985 1105 30-31 0.8651330945279412 1105 32-33 0.9044369078919345 1105 34-35 0.832587169649095 1105 36-37 0.9017668254205518 1105 38-39 0.8738370077167801 1105 40-41 0.7875882688399827 1105 42-43 0.7971104504094839 1105 44-45 0.8789487669938154 1105 46-47 0.9588752973404269 1105 48-49 1.0368204747387892 1105 50-51 0.9345735432285949 1105 52-53 0.9336800121526991 1105 54-55 1.0322932748511704 1105 56-57 0.9158109947732527 1105 58-59 0.9058476342902324 1105 60-61 1.0212929486338567 1105 62-63 0.9856373768824582 1105 64-65 0.9089323137910341 1105 66-67 0.7782167927837449 1105 68-69 0.8374623417705891 1105 70-71 0.8122050524973758 1105 72-73 0.8454645846725128 1105 74-75 0.8339691496517929 1105 76-77 0.8331506963744211 1105 78-79 0.8264853275928381 1105 80-81 0.6993733516326728 1105 82-83 0.7702123196290884 1105 84-85 0.5652732034259387 1105 86-87 0.6944136507262648 1105 88-89 0.7705520293389689 1105 90-91 0.7666995248141504 1105 92-93 0.5210898562382518 1105 94-95 0.6649423907995029 1105 96-97 0.7597909269879821 1105 98-99 0.6821245163401031 1105 100-101 0.7460470800197676 1105 102-103 0.3674547616739865 1105 104-105 0.18688941070917053 1105 106-107 0.6130679189530035 1105 108-109 0.6776257013566998 1105 110-111 0.6839465464350312 1105 112-113 0.5628284144101201 1105 114-115 0.746514231200333 1105 116-117 0.41646716477552204 1105 118-119 0.2901155492945833 1105 120-121 0.29365199396515607 1105 122-123 0.38714774161845966 1105 124-125 0.14261678688317758 1106 1 0.575912024082708 1106 2 0.5079118446762365 1106 3 0.627657540017136 1106 4 0.500460000316771 1106 5 0.4994774464126195 1106 6 0.7311965367024555 1106 7 0.6351177428360231 1106 8 0.6362137205677243 1106 9 0.6346043134021642 1106 10-11 0.8053739878696291 1106 12-13 0.7123670722528246 1106 14-15 0.8926298945732398 1106 16-17 1.0179113773439283 1106 18-19 0.9244630237850942 1106 20-21 1.0154966722566883 1106 22-23 1.0404191709839807 1106 24-25 0.9040099541399798 1106 26-27 0.985931702949884 1106 28-29 1.1221894708272941 1106 30-31 1.0662310926538723 1106 32-33 0.9795800558639556 1106 34-35 0.9673749836560646 1106 36-37 0.9999058754137806 1106 38-39 1.103693639990432 1106 40-41 1.0678962413513489 1106 42-43 1.07494216078382 1106 44-45 0.8831111601712252 1106 46-47 0.8766351744186451 1106 48-49 0.8754786372058021 1106 50-51 0.9407160108412 1106 52-53 1.0687861710755158 1106 54-55 1.0982396161173398 1106 56-57 0.9500242830540131 1106 58-59 0.8938375502034361 1106 60-61 1.015036771339581 1106 62-63 0.9878432884498025 1106 64-65 0.8846950283371875 1106 66-67 0.7887123651734598 1106 68-69 0.8250666503619293 1106 70-71 0.8752434989573104 1106 72-73 0.9062430072583361 1106 74-75 0.8384442620341588 1106 76-77 0.9768239691493221 1106 78-79 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-2.271815935276134 2216 124-125 -2.3453957898509152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 88010.0 3 26995.0 4 12538.0 5 9973.0 6 10606.0 7 12730.0 8 14388.0 9 15196.0 10 16560.0 11 18327.0 12 20501.0 13 22657.0 14 25179.0 15 21147.0 16 14474.0 17 12362.0 18 11538.0 19 10786.0 20 10450.0 21 10936.0 22 13308.0 23 17174.0 24 23700.0 25 31197.0 26 37561.0 27 42580.0 28 49337.0 29 58196.0 30 67289.0 31 79289.0 32 97278.0 33 119653.0 34 154900.0 35 211290.0 36 333665.0 37 524996.0 38 216198.0 39 841.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.17041461643553 20.440405080728613 13.831648475180359 24.5575318276555 2 15.205703799202091 21.315077796435165 39.00618130919625 24.473037095166493 3 19.124890159732608 25.595126237668968 28.972544499260287 26.307439103338133 4 13.001732839148769 17.654541934179715 36.700226133682314 32.64349909298921 5 13.1769858902401 38.71811986799709 33.933379781207776 14.171514460555034 6 31.280966024282115 37.86448433692983 17.108323805325863 13.7462258334622 7 27.885508703415006 32.881727962828194 20.89201125252303 18.34075208123377 8 25.65414064830618 36.85165830899767 19.110156850887144 18.38404419180901 9 25.902339851342788 16.742302202014862 19.96522429477524 37.3901336518671 10-11 24.641431827882403 27.60251027163745 26.97804913410411 20.77800876637604 12-13 25.441389097031724 24.61308434128137 28.016314429453647 21.929212132233257 14-15 22.48950788377583 26.042772967023737 25.940140089717005 25.527579059483436 16-17 21.85160684597848 29.33548721922124 26.50548664257758 22.3074192922227 18-19 21.683053264832157 27.687005692827587 29.20997710043258 21.419963941907668 20-21 22.505113516203522 26.842560529053582 29.278010885832707 21.37431506891019 22-23 22.08238752606044 26.663707467087534 29.19922192129584 22.054683085556185 24-25 22.02593143532057 26.793638295238466 29.321861916831892 21.858568352609073 26-27 21.76929586554131 27.099912533662362 29.28888446934721 21.84190713144912 28-29 21.696033363925054 26.88803024519399 29.4932468102041 21.922689580676863 30-31 21.79346579782085 26.85474296870085 29.451234980041036 21.900556253437266 32-33 21.79810461146808 26.821856329654814 29.295842277307475 22.08419678156963 34-35 22.08516907790998 26.867568659045666 29.152895622827295 21.894366640217065 36-37 22.13870385372815 26.638508220648138 29.157811766883295 22.064976158740414 38-39 21.93432515966158 26.90486868887757 29.2270492185867 21.93375693287415 40-41 21.98238091082695 26.71270656565759 29.2615284082953 22.043384115220157 42-43 22.019401515997117 26.69380714162498 28.981339232260094 22.30545211011781 44-45 22.069887836090924 26.9953385109617 28.736101274248572 22.1986723786988 46-47 22.113494555769723 27.186448905573137 28.346262543119348 22.35379399553779 48-49 22.27492999111736 27.178484244526075 28.19129192795909 22.355293836397475 50-51 22.320210391350397 27.29571661864782 27.95995761813256 22.424115371869227 52-53 22.430637689229897 27.33561787838471 27.797952108164537 22.435792324220856 54-55 22.33705589525145 27.311353780027236 27.858819995900653 22.492770328820665 56-57 22.202945009682352 27.579623185852846 27.81467033531256 22.402761469152242 58-59 22.280030278370244 27.179788984923725 27.989329512684648 22.550851224021383 60-61 22.45896083496868 27.291283198142715 27.97536736876498 22.274388598123636 62-63 22.236987099222542 27.339440418377265 28.085197489249353 22.33837499315084 64-65 22.170145597178973 27.058703198007617 28.064310832443102 22.706840372370305 66-67 22.12518847879601 27.473968110300937 27.87499416552852 22.525849245374534 68-69 22.125805745253096 27.264047003996485 28.069188512578485 22.540958738171934 70-71 22.071978098916105 27.227621504136895 28.01597529025227 22.684425106694725 72-73 22.315534275148945 27.254901284299553 27.86495524140054 22.564609199150958 74-75 22.149151335682543 27.425435798812785 27.76480816896612 22.660604696538552 76-77 22.29985556801829 27.34525412986783 27.83500782027036 22.519882481843517 78-79 22.284062811006862 27.248584690466487 27.901749837241436 22.565602661285215 80-81 22.112852729467075 27.57489187803156 27.78093802238319 22.531317370118174 82-83 22.31903969478042 27.451578861920613 27.678281516356844 22.551099926942122 84-85 22.183506111587224 27.30380081041365 27.755642850576358 22.757050227422763 86-87 22.21371004811081 27.242970843920844 27.962167253194924 22.581151854773427 88-89 22.23179188288034 27.209377365497676 27.882259350882073 22.676571400739913 90-91 22.15195601924665 27.45753012109319 27.910893922205695 22.479619937454466 92-93 22.207760446423045 27.305377433067495 27.8551865017713 22.63167561873816 94-95 22.139836915247315 27.201276816660965 27.85890477738716 22.79998149070456 96-97 22.246508774311838 27.25152066626604 27.801215315302702 22.700755244119424 98-99 22.123920205505446 27.242317594232272 27.656681057838384 22.977081142423895 100-101 22.02293616149071 27.22109591098285 27.882758102845408 22.87320982468103 102-103 22.191725111194945 27.044770412900217 27.85033384358777 22.913170632317065 104-105 22.101385305316324 27.01114015851195 27.92753356720076 22.95994096897096 106-107 22.120785433692504 27.179239163877455 27.848792324259293 22.851183078170752 108-109 22.213756725435797 27.079552442670163 27.543386798560636 23.1633040333334 110-111 22.02151859147938 27.197901270671267 27.71682009305633 23.063760044793025 112-113 22.072928558019655 27.05441483127597 27.8577959075988 23.014860703105576 114-115 21.917862707021037 27.180487618767756 27.643473600321784 23.25817607388943 116-117 21.77868236475488 27.173712967625026 27.758413582280106 23.28919108533999 118-119 21.849386015256467 27.124816640703454 27.5919110036684 23.43388634037168 120-121 21.815620744036323 27.233621072842816 27.654452790897338 23.296305392223523 122-123 21.809777834060167 27.22256756068402 27.60016016128152 23.367494443974294 124-125 21.743189252737114 27.166845626714974 27.567572723363348 23.52239239718456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 233.0 1 184.0 2 264.0 3 464.5 4 385.0 5 322.0 6 751.5 7 1669.0 8 2727.5 9 3230.0 10 2684.0 11 1942.0 12 1742.0 13 1885.5 14 2152.0 15 2395.5 16 2661.0 17 2917.0 18 3130.0 19 3317.0 20 3654.0 21 4237.5 22 5057.0 23 6132.0 24 7542.0 25 9428.0 26 12085.5 27 15604.5 28 19123.0 29 23456.5 30 29225.0 31 34458.5 32 39578.5 33 45484.5 34 51682.5 35 57354.0 36 64263.0 37 70779.5 38 74752.5 39 77608.0 40 78800.0 41 81221.5 42 84248.0 43 87613.5 44 91069.0 45 93057.0 46 92550.5 47 91412.5 48 94297.5 49 94713.0 50 91523.5 51 87980.5 52 84198.5 53 81248.5 54 77533.5 55 71400.0 56 63514.0 57 56939.0 58 52299.5 59 46989.0 60 39653.0 61 31763.5 62 24681.0 63 18737.0 64 14194.0 65 10910.0 66 8391.5 67 6526.0 68 5011.0 69 3666.5 70 2716.5 71 2067.5 72 1626.0 73 1408.0 74 1153.5 75 900.0 76 569.0 77 293.0 78 173.0 79 108.0 80 71.0 81 40.5 82 25.0 83 17.5 84 12.5 85 10.0 86 7.5 87 5.5 88 5.0 89 4.0 90 1.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.060110276584388785 2 0.07577710086634291 3 0.0 4 0.008848102832813473 5 0.033647143341295274 6 6.494020427753008E-4 7 8.11752553469126E-5 8 0.0 9 6.494020427753008E-4 10-11 0.05852735910512398 12-13 0.00888869046048693 14-15 0.006879602890650842 16-17 0.0519115757943506 18-19 1.2176288302036889E-4 20-21 0.009578680130935687 22-23 0.012216875929710345 24-25 0.0 26-27 0.0 28-29 0.051343349006922216 30-31 0.0 32-33 0.0035920050491008823 34-35 0.0 36-37 3.652886490611067E-4 38-39 0.0 40-41 0.0 42-43 0.003247010213876504 44-45 0.0 46-47 1.623505106938252E-4 48-49 4.2617009057129116E-4 50-51 6.291082289385726E-4 52-53 1.2176288302036889E-4 54-55 0.0 56-57 0.0017858556176320772 58-59 0.0 60-61 0.0 62-63 0.0 64-65 8.523401811425823E-4 66-67 0.0 68-69 5.885206012651164E-4 70-71 0.0 72-73 0.0035920050491008823 74-75 0.0056619740604471535 76-77 6.291082289385726E-4 78-79 0.001339391713224058 80-81 0.001481448410081155 82-83 2.029381383672815E-4 84-85 6.0881441510184446E-5 86-87 5.479329735916601E-4 88-89 0.0 90-91 0.0 92-93 0.009030747157344027 94-95 0.007224597725875222 96-97 0.006676664752283562 98-99 0.0010552783195098639 100-101 4.05876276734563E-4 102-103 6.0881441510184446E-5 104-105 6.494020427753008E-4 106-107 0.005966381267998076 108-109 0.008523401811425822 110-111 0.0011567473886935045 112-113 0.003754355559794708 114-115 6.291082289385726E-4 116-117 0.002658489612611388 118-119 0.002557020543427747 120-121 0.014205669685709707 122-123 0.0015626236654280674 124-125 0.001359685527060786 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2463805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.94334888134408 #Duplication Level Percentage of deduplicated Percentage of total 1 78.80389259320683 45.6616144173615 2 12.003893835071194 13.910916168402911 3 3.9498070907511154 6.865951508201956 4 1.7089609706853928 3.960916869960966 5 0.9218585391099398 2.670778547544671 6 0.5289029204718552 1.8387843867157492 7 0.3822419594085859 1.550386544777018 8 0.2703886944116094 1.253378116309041 9 0.20437788455945632 1.065810516179368 >10 1.0922948066444451 11.762314800693103 >50 0.0865996485686952 3.450441830298434 >100 0.04481211741814275 4.47260687392237 >500 0.0014063854948859968 0.5080129327324928 >1k 4.2191564846453454E-4 0.46547479495680116 >5k 1.406385494881782E-4 0.5626116919435845 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7455 0.3025807643056167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6353 0.25785319860946787 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4066 0.1650292941202733 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.05876276734563E-5 0.0 10-11 4.05876276734563E-5 0.0 0.0 4.05876276734563E-5 0.0 12-13 4.05876276734563E-5 0.0 0.0 4.05876276734563E-5 0.0 14-15 4.05876276734563E-5 0.0 0.0 4.05876276734563E-5 0.0 16-17 4.05876276734563E-5 0.0 0.0 4.05876276734563E-5 0.0 18-19 4.05876276734563E-5 0.0 0.0 4.05876276734563E-5 0.0 20-21 4.05876276734563E-5 0.0 0.0 6.0881441510184446E-5 0.0 22-23 4.05876276734563E-5 0.0 0.0 1.2176288302036889E-4 0.0 24-25 4.05876276734563E-5 0.0 0.0 2.6381957987746597E-4 0.0 26-27 4.05876276734563E-5 0.0 0.0 3.8558246289783485E-4 0.0 28-29 4.05876276734563E-5 0.0 0.0 8.320463673058543E-4 0.0 30-31 4.05876276734563E-5 0.0 0.0 0.0023540824050604656 0.0 32-33 4.05876276734563E-5 0.0 0.0 0.005560504991263513 0.0 34-35 4.05876276734563E-5 0.0 0.0 0.009172803854201123 0.0 36-37 4.05876276734563E-5 0.0 0.0 0.015890056234158144 0.0 38-39 4.05876276734563E-5 0.0 0.0 0.028248988860725584 0.0 40-41 4.05876276734563E-5 0.0 0.0 0.04206907608353745 0.0 42-43 4.05876276734563E-5 0.0 0.0 0.05479329735916601 0.0 44-45 4.05876276734563E-5 0.0 0.0 0.07041953401344668 0.0 46-47 4.05876276734563E-5 0.0 0.0 0.08975953859984862 0.0 48-49 4.05876276734563E-5 0.0 0.0 0.11052011015482151 0.0 50-51 4.05876276734563E-5 0.0 0.0 0.134446516668324 0.0 52-53 4.05876276734563E-5 0.0 0.0 0.15835262936798977 0.0 54-55 4.05876276734563E-5 0.0 0.0 0.18479546879724654 0.0 56-57 4.05876276734563E-5 0.0 0.0 0.21515501429699185 0.0 58-59 4.05876276734563E-5 0.0 0.0 0.25105477097416395 0.0 60-61 4.05876276734563E-5 0.0 0.0 0.29245415120108936 0.0 62-63 4.05876276734563E-5 0.0 0.0 0.3367555468066669 0.0 64-65 4.05876276734563E-5 0.0 0.0 0.3820310454764074 0.0 66-67 4.05876276734563E-5 0.0 0.0 0.4338005645739009 0.0 68-69 4.05876276734563E-5 0.0 0.0 0.49348467106771843 0.0 70-71 4.05876276734563E-5 0.0 0.0 0.5628083391339818 0.0 72-73 4.05876276734563E-5 0.0 0.0 0.6352572545311013 0.0 74-75 4.05876276734563E-5 0.0 0.0 0.7079699895080982 0.0 76-77 4.05876276734563E-5 0.0 0.0 0.788678487136766 0.0 78-79 4.05876276734563E-5 0.0 0.0 0.8859061492285307 0.0 80-81 4.05876276734563E-5 0.0 0.0 1.0014997128425343 0.0 82-83 4.05876276734563E-5 0.0 0.0 1.122369668054087 0.0 84-85 8.11752553469126E-5 0.0 0.0 1.2463039891549859 0.0 86-87 8.11752553469126E-5 0.0 0.0 1.3766917430559644 0.0 88-89 8.11752553469126E-5 0.0 0.0 1.5276980118150583 0.0 90-91 8.11752553469126E-5 0.0 0.0 1.7050050633065523 0.0 92-93 8.11752553469126E-5 0.0 0.0 1.8956451504887766 0.0 94-95 8.11752553469126E-5 0.0 0.0 2.084458794425695 0.0 96-97 8.11752553469126E-5 0.0 0.0 2.2785285361463266 0.0 98-99 8.11752553469126E-5 0.0 0.0 2.49528676173642 0.0 100-101 8.11752553469126E-5 0.0 0.0 2.751029403706868 0.0 102-103 8.11752553469126E-5 0.0 0.0 3.0290140656423703 0.0 104-105 8.11752553469126E-5 0.0 0.0 3.3050505214495463 0.0 106-107 8.11752553469126E-5 0.0 0.0 3.586383662668109 0.0 108-109 8.11752553469126E-5 0.0 0.0 3.886001530153563 0.0 110-111 8.11752553469126E-5 0.0 0.0 4.232295169463493 0.0 112-113 8.11752553469126E-5 0.0 0.0 4.619237317888388 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5805 0.0 50.99919 1 GTATCAA 9505 0.0 45.999493 1 ATCAACG 11640 0.0 37.06181 3 TCAACGC 11795 0.0 36.628193 4 CAACGCA 11965 0.0 35.54704 5 TATCAAC 12210 0.0 35.30657 2 AACGCAG 12300 0.0 34.663773 6 GTACATG 10630 0.0 30.316082 1 ACGCAGA 14440 0.0 29.36226 7 CGCAGAG 14615 0.0 28.969934 8 TACATGG 11110 0.0 28.297787 2 ACATGGG 10860 0.0 27.669613 3 GAGTACT 8605 0.0 26.020912 12-13 GCAGAGT 15830 0.0 25.994574 9 CATGGGG 4565 0.0 23.985783 4 GTACTTT 9355 0.0 23.90296 14-15 AGTACTT 9350 0.0 22.705626 12-13 CAGAGTA 15605 0.0 22.010876 10-11 ATACCGT 325 6.039045E-10 21.98699 6 AGAGTAC 14785 0.0 21.942106 10-11 >>END_MODULE