FastQCFastQC Report
Thu 26 May 2016
SRR1364635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364000
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96880.2879904875148633No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT54130.16090963139120093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44900.13347205707491083No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT44160.13127229488703923No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC36100.1073127229488704No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG185750.033.1586841
TACATGG186700.032.350892
ACATGGG198850.028.9962883
CATGGGA138100.024.1535224
ATGGGAG52750.022.1332725
CATGGGG64600.021.483724
GTTATCA24150.020.7189481
GTGTAGG18050.020.1306671
ATGGGAT51650.019.9520655
AGTACAT143650.018.9082722
GAGTACT56950.018.5949412-13
TACCTGG32600.017.9061032
GTACTTT60550.017.58802414-15
GTACTAG9250.017.3871171
ACTATAC11400.017.2425583
GTACCTG36800.017.1578791
GTATATA24950.016.9509241
TAGCCCT25450.016.85124
GAGTACA206000.016.655621
GTATAGA13950.016.6531331
Produced by FastQC (version 0.11.5)