##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364635_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3364000 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.78096046373365 31.0 26.0 31.0 10.0 34.0 2 26.41669857312723 31.0 26.0 31.0 10.0 34.0 3 26.866094530321046 31.0 26.0 31.0 10.0 34.0 4 30.07702675386445 35.0 30.0 37.0 10.0 37.0 5 30.437533590963138 35.0 31.0 37.0 7.0 37.0 6 30.513349583828774 35.0 31.0 37.0 8.0 37.0 7 30.255328180737216 35.0 30.0 37.0 8.0 37.0 8 30.088343043995245 35.0 30.0 37.0 8.0 37.0 9 30.96576753864447 37.0 30.0 39.0 7.0 39.0 10-11 30.910515160523186 37.0 29.5 39.0 7.0 39.0 12-13 30.96304131985731 37.0 30.0 39.0 2.0 39.0 14-15 31.624326545778835 38.0 29.5 40.0 2.0 41.0 16-17 32.06641319857313 38.0 31.0 40.0 2.0 41.0 18-19 31.90350029726516 38.0 30.0 40.0 2.0 41.0 20-21 31.78479756242568 38.0 30.0 40.0 2.0 41.0 22-23 31.873626783590964 38.0 30.0 40.0 2.0 41.0 24-25 31.975387485136743 38.0 30.0 40.0 2.0 41.0 26-27 31.771563763376932 38.0 30.0 40.0 2.0 41.0 28-29 31.676196195005943 38.0 30.0 40.0 2.0 41.0 30-31 31.556201397146253 38.0 30.0 40.0 2.0 41.0 32-33 31.441992271105825 37.5 30.0 40.0 2.0 41.0 34-35 31.482054399524376 38.0 30.0 40.0 2.0 41.0 36-37 31.429235285374553 38.0 30.0 40.0 2.0 41.0 38-39 31.131443668252082 37.0 29.0 40.0 2.0 41.0 40-41 31.0049555588585 37.0 29.0 40.0 2.0 41.0 42-43 30.515252972651606 37.0 27.5 40.0 2.0 41.0 44-45 30.39834690844233 37.0 28.0 40.0 2.0 41.0 46-47 30.363537752675384 36.5 28.0 40.0 2.0 41.0 48-49 30.275894768133174 37.0 28.0 40.0 2.0 41.0 50-51 30.04190487514863 36.0 27.0 40.0 2.0 41.0 52-53 29.936987960761 36.0 27.5 40.0 2.0 41.0 54-55 29.75140948275862 36.0 27.0 40.0 2.0 41.0 56-57 29.50902318668252 35.5 26.5 40.0 2.0 41.0 58-59 29.286595422116527 35.0 26.0 39.0 2.0 41.0 60-61 28.96535151605232 35.0 26.0 39.0 2.0 41.0 62-63 28.785445154577886 35.0 26.0 39.0 2.0 40.0 64-65 28.543383174791913 34.0 26.0 39.0 2.0 40.0 66-67 28.253668697978597 34.0 26.0 38.0 2.0 40.0 68-69 27.987479785969086 34.0 25.0 38.0 2.0 40.0 70-71 27.536504756242568 34.0 24.0 37.0 2.0 40.0 72-73 27.291097948870394 33.5 24.0 36.5 2.0 39.0 74-75 27.25475014863258 34.0 24.5 36.0 2.0 39.0 76-77 26.987537901307967 33.0 24.0 36.0 2.0 39.0 78-79 26.679371135552913 33.0 23.0 35.5 2.0 38.0 80-81 26.258775416171225 33.0 22.0 35.0 2.0 37.0 82-83 26.35364863258026 33.0 23.0 35.0 2.0 37.0 84-85 26.19262604042806 33.0 23.0 35.0 2.0 36.5 86-87 26.066100178359097 33.0 23.0 35.0 2.0 36.0 88-89 25.883867271105828 33.0 22.0 35.0 2.0 36.0 90-91 25.732421670630202 33.0 20.5 35.0 2.0 35.5 92-93 25.59598900118906 33.0 20.0 35.0 2.0 35.0 94-95 25.48980677764566 33.0 20.0 35.0 2.0 35.0 96-97 25.371252378121284 33.0 19.5 35.0 2.0 35.0 98-99 25.289366082045184 33.0 19.0 35.0 2.0 35.0 100-101 25.178827288941733 33.0 18.0 35.0 2.0 35.0 102-103 24.043128864447084 30.5 16.0 34.0 2.0 35.0 104-105 24.857819262782403 32.5 17.0 35.0 2.0 35.0 106-107 25.000321195005945 33.0 17.0 35.0 2.0 35.0 108-109 24.971305885850178 33.0 15.5 35.0 2.0 35.0 110-111 24.906046224732464 33.0 11.5 35.0 2.0 35.0 112-113 24.795879161712246 33.0 8.5 35.0 2.0 35.0 114-115 24.67479875148633 33.0 4.5 35.0 2.0 35.0 116-117 24.55330737217598 33.0 2.0 35.0 2.0 35.0 118-119 24.43215324019025 33.0 2.0 35.0 2.0 35.0 120-121 24.243611028537458 33.0 2.0 35.0 2.0 35.0 122-123 24.032001783590964 33.0 2.0 35.0 2.0 35.0 124-125 23.073073424494652 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.5037976572311855 1101 2 1.7979833498117692 1101 3 1.4602940120404284 1101 4 1.572110186764256 1101 5 1.3674563454070032 1101 6 1.2991089407166037 1101 7 1.353508701917324 1101 8 1.496990959317472 1101 9 2.063906983345074 1101 10-11 2.0247768398162016 1101 12-13 1.9728402195683046 1101 14-15 2.3958532790863316 1101 16-17 1.8969576029868307 1101 18-19 1.9345313563497335 1101 20-21 2.1700243409889914 1101 22-23 1.9843350675177227 1101 24-25 1.8448850719614498 1101 26-27 2.172336431214063 1101 28-29 2.099495514369263 1101 30-31 2.163981929021201 1101 32-33 2.1730369408488848 1101 34-35 2.0111007277455926 1101 36-37 1.795568682353199 1101 38-39 1.8325579109194408 1101 40-41 1.9251940013841597 1101 42-43 2.172223912086153 1101 44-45 1.9919391678170584 1101 46-47 1.7338263969463164 1101 48-49 1.5624952282380065 1101 50-51 1.6345180706933533 1101 52-53 1.486826414405531 1101 54-55 1.509171308100452 1101 56-57 1.539955912776822 1101 58-59 1.3658495733882532 1101 60-61 1.5003272198852287 1101 62-63 1.2978345195976715 1101 64-65 1.2692848464418063 1101 66-67 1.392420773539257 1101 68-69 1.1892841639185434 1101 70-71 1.1851392980040139 1101 72-73 1.2065648134062492 1101 74-75 1.1128927088871805 1101 76-77 1.2182204291130887 1101 78-79 1.2120641351742591 1101 80-81 1.426535463774691 1101 82-83 1.3623736189653997 1101 84-85 1.399229602078421 1101 86-87 1.288085625636274 1101 88-89 1.245110947648115 1101 90-91 1.2650615406673609 1101 92-93 1.2367088886399102 1101 94-95 1.1533514008857715 1101 96-97 1.199350723407381 1101 98-99 1.2572392019887495 1101 100-101 1.2499509509451308 1101 102-103 1.124711863952438 1101 104-105 1.270273448274768 1101 106-107 1.2865778480469245 1101 108-109 1.2320660903658123 1101 110-111 1.358187195943394 1101 112-113 1.2937855382217194 1101 114-115 1.2177947997555698 1101 116-117 1.1999611584726502 1101 118-119 1.1537820205541394 1101 120-121 1.178765352667419 1101 122-123 1.1020597510532433 1101 124-125 0.9058888974845232 1102 1 0.7641844675266363 1102 2 1.355245533001117 1102 3 1.0155973090190926 1102 4 0.983725533287096 1102 5 0.8201181366102865 1102 6 0.6633733254715075 1102 7 0.6694353730786062 1102 8 0.7496009182693122 1102 9 1.3209983811303836 1102 10-11 1.2733338523096442 1102 12-13 1.3498762575259207 1102 14-15 1.5807513373532416 1102 16-17 1.2392750937987316 1102 18-19 0.9889664444720161 1102 20-21 1.2929629089571115 1102 22-23 1.2529267740591798 1102 24-25 0.9885550489315236 1102 26-27 1.4085440928411899 1102 28-29 1.0781955167557875 1102 30-31 1.066552435798922 1102 32-33 1.2600158589196262 1102 34-35 1.1185401474033725 1102 36-37 1.006876055807929 1102 38-39 1.2936568739760972 1102 40-41 1.2514627728628405 1102 42-43 1.455426459100618 1102 44-45 1.161601154416747 1102 46-47 0.9813116750953306 1102 48-49 0.8698763272905587 1102 50-51 1.010846393327121 1102 52-53 0.9418239383900655 1102 54-55 0.8865976168088672 1102 56-57 0.9408543260387177 1102 58-59 0.9959406564511113 1102 60-61 0.9781972723290266 1102 62-63 0.7657164598988508 1102 64-65 0.6958754999309029 1102 66-67 0.7797063222600826 1102 68-69 0.6686070855571273 1102 70-71 0.8210945104640395 1102 72-73 0.5032155923070185 1102 74-75 0.6654478151470222 1102 76-77 0.5435980587608391 1102 78-79 0.631582333887927 1102 80-81 0.8180479885348468 1102 82-83 0.7983210379430155 1102 84-85 0.7342372538611528 1102 86-87 0.8867349293687923 1102 88-89 0.7257531678882003 1102 90-91 0.8283024062585014 1102 92-93 0.8552625227146571 1102 94-95 0.8449520777627697 1102 96-97 0.5837998920031495 1102 98-99 0.740262051435078 1102 100-101 0.768538592943127 1102 102-103 0.726688984674599 1102 104-105 0.8015411234454071 1102 106-107 0.6765144859162433 1102 108-109 0.8581565066943888 1102 110-111 0.8639448268440653 1102 112-113 0.6865587053736419 1102 114-115 0.8005867160125497 1102 116-117 0.9193506715030573 1102 118-119 0.8783609487675896 1102 120-121 0.8699403711629614 1102 122-123 0.7469691527524454 1102 124-125 0.7414210245309611 1103 1 1.0868201262650707 1103 2 1.450631413189555 1103 3 1.0519312567648527 1103 4 0.8275440932423379 1103 5 0.7777961503866173 1103 6 0.9116912719619101 1103 7 0.8100511424415977 1103 8 1.0156256926215157 1103 9 1.5808231238712374 1103 10-11 1.557821663178263 1103 12-13 1.6156633213584826 1103 14-15 1.7628675579549693 1103 16-17 1.372365258537819 1103 18-19 1.4129373226117856 1103 20-21 1.6237360452236267 1103 22-23 1.4844225177085697 1103 24-25 1.3956757515979845 1103 26-27 1.5889635155512565 1103 28-29 1.610431530723904 1103 30-31 1.6356560759177476 1103 32-33 1.7300223934478218 1103 34-35 1.5071614832443814 1103 36-37 1.323116082977009 1103 38-39 1.325356916389886 1103 40-41 1.4763535071368672 1103 42-43 1.5859369065755544 1103 44-45 1.40480535880193 1103 46-47 1.2740277608371322 1103 48-49 1.2080136190924904 1103 50-51 1.1537964182106322 1103 52-53 0.9544018249250357 1103 54-55 0.9956639904072482 1103 56-57 0.9155088659249238 1103 58-59 0.9310803850559353 1103 60-61 0.9971276943860126 1103 62-63 0.7650876063181435 1103 64-65 0.6242038357146811 1103 66-67 0.6749761046534708 1103 68-69 0.6612423993755705 1103 70-71 0.7474544143867661 1103 72-73 0.6951082478990962 1103 74-75 0.673066282845646 1103 76-77 0.7702787951309062 1103 78-79 0.7365544882316613 1103 80-81 0.8254714058732553 1103 82-83 0.8129869033340071 1103 84-85 0.8253483813970242 1103 86-87 0.7684578464215974 1103 88-89 0.7791736181566122 1103 90-91 0.87335812978883 1103 92-93 0.864188237182308 1103 94-95 0.7883914910500742 1103 96-97 0.8396585665324743 1103 98-99 0.8175947623962969 1103 100-101 0.7824123363067308 1103 102-103 0.68192324030883 1103 104-105 0.7603780873459272 1103 106-107 0.9176940099493933 1103 108-109 0.9146562207140185 1103 110-111 0.8924489960170909 1103 112-113 0.9311109404741416 1103 114-115 1.0231688363456577 1103 116-117 1.0075473767211633 1103 118-119 1.0025335292959845 1103 120-121 0.9584945423638089 1103 122-123 0.8465809661484549 1103 124-125 0.7420831382537365 1104 1 1.1800595791931308 1104 2 1.645743729308851 1104 3 1.1513874362599559 1104 4 1.3003616799612026 1104 5 0.9304480407015348 1104 6 0.9070499235795317 1104 7 0.7446361107979094 1104 8 1.1434704603646324 1104 9 1.4580480802530325 1104 10-11 1.5429584694998688 1104 12-13 1.4547297222655047 1104 14-15 1.4521692988273216 1104 16-17 1.3217999747969529 1104 18-19 1.3385568478336758 1104 20-21 1.544952078944295 1104 22-23 1.4667354333538931 1104 24-25 1.516720679490291 1104 26-27 1.7203489115943498 1104 28-29 1.7004534984542694 1104 30-31 1.7502470315334584 1104 32-33 1.6824192362908228 1104 34-35 1.5944032868496052 1104 36-37 1.5178793645418907 1104 38-39 1.5607212174556864 1104 40-41 1.5142630935192507 1104 42-43 1.817751689572404 1104 44-45 1.5051909080824188 1104 46-47 1.3900639706441247 1104 48-49 1.2817305281776044 1104 50-51 1.2361990760737598 1104 52-53 1.0857953884433797 1104 54-55 1.1866587877810808 1104 56-57 1.1361921024542312 1104 58-59 1.1635095622111393 1104 60-61 1.163942209022963 1104 62-63 1.0852466353953538 1104 64-65 1.0134716510061743 1104 66-67 0.9577033724307569 1104 68-69 0.935455824282414 1104 70-71 1.0034420349389777 1104 72-73 0.9589640918498716 1104 74-75 0.9835008492010502 1104 76-77 0.9481289022915149 1104 78-79 0.9791165018710117 1104 80-81 1.0654416073676458 1104 82-83 1.114710273947697 1104 84-85 1.1542529985096728 1104 86-87 1.0236127734260378 1104 88-89 1.0701004199099842 1104 90-91 0.9471176542039679 1104 92-93 0.9998496121028708 1104 94-95 1.030819880349501 1104 96-97 0.9945815705765426 1104 98-99 0.9777564517368766 1104 100-101 1.0859321069862524 1104 102-103 0.9648854757923644 1104 104-105 1.0572505888346875 1104 106-107 1.0511107182035104 1104 108-109 1.0133099318758703 1104 110-111 1.0215397204432506 1104 112-113 1.007489001902222 1104 114-115 1.0143069590198017 1104 116-117 1.0242078038812288 1104 118-119 0.9962476292140998 1104 120-121 0.952864762793407 1104 122-123 0.939952043218419 1104 124-125 0.7719990443728868 1105 1 0.9293654154054849 1105 2 1.321348947308703 1105 3 0.6487183094257496 1105 4 0.9136007613896204 1105 5 0.7097667229251599 1105 6 0.5978754196839056 1105 7 0.6137227169302228 1105 8 0.7471482013718855 1105 9 0.9714763871289449 1105 10-11 0.8784554605003443 1105 12-13 1.0362338547530143 1105 14-15 1.2997550119509569 1105 16-17 0.8347703717277994 1105 18-19 0.9550327898254842 1105 20-21 0.8417110793656555 1105 22-23 1.0856833609554108 1105 24-25 0.6822929281175618 1105 26-27 0.9798905253397265 1105 28-29 1.0784519531740848 1105 30-31 0.917725576442205 1105 32-33 1.085151068847356 1105 34-35 0.8831593721704252 1105 36-37 0.48339338719556935 1105 38-39 0.832675985407576 1105 40-41 0.9427804530656765 1105 42-43 1.112618166518665 1105 44-45 0.8475760238679655 1105 46-47 0.3841485144421526 1105 48-49 0.5429899946506467 1105 50-51 0.6997107694015696 1105 52-53 0.484914065129189 1105 54-55 0.5339187058873982 1105 56-57 0.6980638705970712 1105 58-59 0.774854954497247 1105 60-61 0.6756727664471711 1105 62-63 0.6438807539077729 1105 64-65 0.425574228429074 1105 66-67 0.27656647173972004 1105 68-69 0.17248435135107343 1105 70-71 0.4949566770820155 1105 72-73 0.3833489818187772 1105 74-75 0.4905485986599274 1105 76-77 0.503927598092833 1105 78-79 0.5857363689720358 1105 80-81 0.7136590977700799 1105 82-83 0.649539065052533 1105 84-85 0.5450477353464933 1105 86-87 0.7370086891003105 1105 88-89 0.7113024050017813 1105 90-91 0.5135305748466372 1105 92-93 0.6295517163437907 1105 94-95 0.505922322770207 1105 96-97 0.5285200250696853 1105 98-99 0.6614805776909094 1105 100-101 0.7140947593819966 1105 102-103 0.6117267436251019 1105 104-105 0.6993245467963511 1105 106-107 0.7128387577865567 1105 108-109 0.6785173087344276 1105 110-111 0.6961189540353558 1105 112-113 0.6278188369427369 1105 114-115 0.6809831482908741 1105 116-117 0.6668907243244782 1105 118-119 0.6590261860162379 1105 120-121 0.5407621495038271 1105 122-123 0.5198504601634433 1105 124-125 0.5192041689472084 1106 1 0.6275618164102248 1106 2 -1.571736603783446 1106 3 0.11905050304834575 1106 4 0.591561183843158 1106 5 0.5784252604987259 1106 6 0.7399317907977192 1106 7 0.848656947929701 1106 8 0.8873762657785527 1106 9 1.342105819643411 1106 10-11 1.4942064349125026 1106 12-13 1.4916187711924707 1106 14-15 1.6396539079370314 1106 16-17 1.2515198831534065 1106 18-19 1.1840318744459815 1106 20-21 1.5912922353141177 1106 22-23 1.2422699599928926 1106 24-25 1.343191292987349 1106 26-27 1.3217967452147619 1106 28-29 1.4117954688426586 1106 30-31 1.5063681890566976 1106 32-33 1.6223179252288737 1106 34-35 1.3936338320304102 1106 36-37 1.257374990185859 1106 38-39 1.51939241650512 1106 40-41 1.4794261089367247 1106 42-43 1.7476856706033317 1106 44-45 1.4788385509835429 1106 46-47 1.2267697368313186 1106 48-49 1.1771480900300908 1106 50-51 1.2355023885684417 1106 52-53 1.1679973928115928 1106 54-55 1.040490150482487 1106 56-57 1.1159774557301354 1106 58-59 1.0179250312830739 1106 60-61 1.18238599961618 1106 62-63 1.046775199093826 1106 64-65 1.081456522623995 1106 66-67 1.0763618227946523 1106 68-69 0.9638399148013406 1106 70-71 1.0437337413025887 1106 72-73 0.8827207335116753 1106 74-75 0.9822723503767072 1106 76-77 0.84862196526052 1106 78-79 1.0350587798760742 1106 80-81 1.141935297007496 1106 82-83 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-1.683710071040089 2216 82-83 -1.7607958219529856 2216 84-85 -1.7020221640986293 2216 86-87 -1.9147773599522253 2216 88-89 -1.943869248816256 2216 90-91 -1.7248795930608942 2216 92-93 -1.755666877647954 2216 94-95 -1.9491316126467346 2216 96-97 -1.9645722690695244 2216 98-99 -1.9912130712479659 2216 100-101 -2.0573300903248573 2216 102-103 -1.8952914684867856 2216 104-105 -1.826494872742547 2216 106-107 -1.816061280110155 2216 108-109 -1.907557997266796 2216 110-111 -1.7677322550494416 2216 112-113 -2.051092642785928 2216 114-115 -1.9815507859981487 2216 116-117 -1.7721882601056436 2216 118-119 -1.834597258568575 2216 120-121 -1.9750051519869771 2216 122-123 -1.859841039539237 2216 124-125 -1.7424042938872155 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 302880.0 3 38125.0 4 22844.0 5 18869.0 6 18750.0 7 21053.0 8 22731.0 9 24348.0 10 26262.0 11 28010.0 12 29966.0 13 31356.0 14 31232.0 15 27081.0 16 22853.0 17 19876.0 18 18149.0 19 17194.0 20 16614.0 21 17543.0 22 21266.0 23 29409.0 24 42398.0 25 55670.0 26 61693.0 27 65329.0 28 74601.0 29 85487.0 30 99619.0 31 115915.0 32 139477.0 33 176859.0 34 230100.0 35 310666.0 36 446168.0 37 512087.0 38 141276.0 39 244.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84384000324049 18.727583887860018 13.904973852709054 22.523602256190436 2 13.278315757860696 20.946662209486284 41.9988249251724 23.776197107480616 3 19.93167536348695 26.59799228940888 27.79362871721255 25.676703629891627 4 11.90433116968077 17.041841180013726 37.72727963632278 33.32654801398272 5 13.159104528339013 40.40218113952317 32.753614114799014 13.685100217338809 6 31.112860765723134 37.48883254643561 17.45024283160448 13.948063856236779 7 28.28292035168897 34.23394280481989 19.922547883322 17.56058896016914 8 25.388063124100984 36.471620064148574 19.296837540911838 18.843479270838607 9 25.8227879013131 18.56210458352542 19.93217687974336 35.68293063541812 10-11 24.078137594460316 28.57873171068564 26.753208406208813 20.589922288645237 12-13 25.673163735207797 25.7068660222444 26.911201767881455 21.708768474666346 14-15 22.295139470987827 26.99061458828686 25.79070582500595 24.923540115719366 16-17 21.773844237149632 30.172841601062174 25.874515687771737 22.178798474016453 18-19 21.792700605605482 29.341441819546926 27.567741477312907 21.298116097534685 20-21 23.329903707970402 27.666804179210157 27.19260177553457 21.81069033728487 22-23 22.48306627323399 27.74840996892009 27.037839701994997 22.730684055850922 24-25 22.673597178164123 27.549989242420747 27.35020350064285 22.426210078772282 26-27 22.58995075617581 27.187769024015324 27.912430993175402 22.309849226633467 28-29 22.053053697389622 28.164929312946374 27.64975222910442 22.13226476055959 30-31 22.326153483449392 27.536075497298086 27.531759222725277 22.606011796527245 32-33 22.2635890907602 27.51978202365133 27.5787577283708 22.637871157217678 34-35 22.959980996911998 27.161631026946644 27.479822614031924 22.398565362109437 36-37 22.744680838397148 27.079844427285593 27.73963835833042 22.435836375986838 38-39 22.508395705293584 27.356175765729862 27.842461924358037 22.29296660461852 40-41 22.528574366317443 27.555223721909773 27.712604796005447 22.203597115767337 42-43 22.05746670171279 27.59974585600454 27.469395734505355 22.87339170777732 44-45 22.493453468080272 27.35599602184028 27.176633477128036 22.97391703295141 46-47 22.816390823522653 27.74016005982187 26.833961116676907 22.609487999978565 48-49 22.743729543150835 27.54761915393672 26.964936644235767 22.74371465867668 50-51 22.386523415462925 27.77207839975755 27.425476497607505 22.415921687172023 52-53 22.771841347972618 28.044192982594573 26.63583160644788 22.548134062984932 54-55 22.352625763124944 27.596991727264864 26.994277279311728 23.05610523029846 56-57 22.75421299746953 27.607673016586126 26.747262428057837 22.890851557886506 58-59 23.19401673632695 27.33020640914289 26.705823663144663 22.7699531913855 60-61 22.836784911807328 27.424197049567223 27.33966890090485 22.399349137720602 62-63 22.682568843385873 27.622543518806804 27.11070901082337 22.584178626983952 64-65 22.442749727507216 27.957551249776024 26.910009221001026 22.689689801715737 66-67 22.65503375918084 27.58339501262305 26.622730775034125 23.138840453161983 68-69 22.493062895766876 27.648938106313032 26.84112837113802 23.016870626782083 70-71 22.855350321997307 27.462435631789134 26.796669966303234 22.885544079910332 72-73 22.876529575500072 27.23122341789073 26.91685396668636 22.975393039922835 74-75 22.451073146614746 28.091194736025376 26.6227307805667 22.835001336793177 76-77 22.809955382062018 27.357151690125942 26.772890781960502 23.060002145851534 78-79 22.844565933826352 27.48622194627835 26.83829290684528 22.830919213050016 80-81 22.833317656247953 27.60859755175522 26.802636607939114 22.755448184057713 82-83 22.8270399967375 27.602435742359503 26.897530248454594 22.672994012448402 84-85 22.759708906548262 27.54622316202278 26.841586045896282 22.85248188553267 86-87 22.53154985010286 27.662646585113887 26.935042587459847 22.870760977323407 88-89 22.722289757122933 27.54424197535789 26.625205017253528 23.108263250265654 90-91 22.769910218942904 27.378221679020665 26.813510676867374 23.038357425169057 92-93 22.853577710314216 27.66512904117834 26.874849866453697 22.606443382053744 94-95 22.633700442889413 27.621595116426683 26.834346973311117 22.91035746737278 96-97 22.307142274527735 27.608195668184273 27.057877411484387 23.026784645803602 98-99 22.53203265836832 27.767159098215625 26.481640681744032 23.219167561672023 100-101 22.327331536901553 27.561626598142734 26.813872286676705 23.29716957827901 102-103 22.62995703640034 27.25771773535786 26.989048234422476 23.12327699381932 104-105 22.779609656347052 27.26378947233713 26.859142633211814 23.097458238104004 106-107 22.39940807312892 27.665214876329458 27.154719618247647 22.780657432293975 108-109 22.18513368987141 27.623247187328936 26.90735814454943 23.284260978250227 110-111 21.99396332313333 28.049016566266182 26.745856201506268 23.211163909094225 112-113 22.52811515567837 27.634363343315187 26.588805535680628 23.248715965325818 114-115 22.261065463648645 27.669439237958983 26.759855730676634 23.309639567715738 116-117 22.28805703887648 27.71308861885753 26.777651639704033 23.221202702561957 118-119 22.20322508086802 27.52810743842963 27.141990912315027 23.126676568387328 120-121 22.013456643003273 28.19671136198417 26.6934266362156 23.096405358796957 122-123 22.064448778618097 27.7005327383202 26.60919588117275 23.625822601888956 124-125 22.28347909407147 27.575263425709633 26.747477332444586 23.393780147774308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6002.0 1 4536.0 2 2448.5 3 1551.5 4 1131.5 5 945.5 6 976.5 7 1225.0 8 1531.5 9 1657.0 10 1583.0 11 1511.5 12 1575.0 13 1763.0 14 1981.5 15 2179.0 16 2360.0 17 2597.0 18 2953.0 19 3398.5 20 4055.0 21 4882.0 22 5971.0 23 7598.5 24 9713.0 25 12712.0 26 16690.5 27 21531.0 28 26234.0 29 31613.0 30 38518.5 31 44471.5 32 50511.0 33 57166.0 34 63953.0 35 71205.0 36 78667.5 37 85760.5 38 90911.0 39 94023.0 40 97687.0 41 102968.5 42 107813.0 43 112605.5 44 117889.5 45 122176.5 46 126534.5 47 139054.5 48 162062.5 49 161139.0 50 137900.0 51 122873.0 52 113828.5 53 108258.0 54 104045.0 55 98349.5 56 89872.0 57 81633.0 58 75188.0 59 67121.0 60 56405.0 61 45535.0 62 35999.5 63 27898.0 64 21030.5 65 16021.5 66 12324.0 67 9557.5 68 7446.0 69 5688.0 70 4376.0 71 3469.0 72 2761.0 73 2388.0 74 1924.5 75 1412.0 76 1038.0 77 684.5 78 478.0 79 361.0 80 274.0 81 209.0 82 145.5 83 112.5 84 87.5 85 64.5 86 55.5 87 42.5 88 31.0 89 20.5 90 11.5 91 11.5 92 10.5 93 8.0 94 8.0 95 7.0 96 3.5 97 1.5 98 2.0 99 1.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19268727705112962 2 0.22645659928656361 3 0.27152199762187873 4 0.2591260404280618 5 0.2910225921521998 6 0.21245541022592151 7 0.2030023781212842 8 0.18341260404280618 9 0.18275862068965518 10-11 0.13043995243757434 12-13 0.1539090368608799 14-15 0.16953032104637336 16-17 0.14432223543400713 18-19 0.16722651605231867 20-21 0.16404577883472057 22-23 0.18442330558858502 24-25 0.17546076099881094 26-27 0.2240933412604043 28-29 0.15612366230677766 30-31 0.13738109393579073 32-33 0.17352853745541022 34-35 0.13513674197384065 36-37 0.1350178359096314 38-39 0.16606718192627826 40-41 0.1850921521997622 42-43 0.25035671819262784 44-45 0.1534036860879905 46-47 0.13952140309155767 48-49 0.142538644470868 50-51 0.14741379310344827 52-53 0.16627526753864447 54-55 0.15444411414982165 56-57 0.15274970273483948 58-59 0.12966706302021402 60-61 0.14161712247324612 62-63 0.13133174791914387 64-65 0.12717003567181928 66-67 0.11524970273483948 68-69 0.118564209274673 70-71 0.1199167657550535 72-73 0.09823127229488704 74-75 0.09945005945303209 76-77 0.11975326991676577 78-79 0.12540130796670632 80-81 0.1347205707491082 82-83 0.13714328180737217 84-85 0.12442033293697978 86-87 0.1495243757431629 88-89 0.14362366230677764 90-91 0.13359096313912008 92-93 0.13350178359096315 94-95 0.15303210463733652 96-97 0.1415576694411415 98-99 0.13721759809750297 100-101 0.11908442330558859 102-103 0.15536563614744353 104-105 0.11621581450653984 106-107 0.12263674197384067 108-109 0.13290725326991676 110-111 0.11021105826397146 112-113 0.10111474435196195 114-115 0.09931629013079668 116-117 0.10359690844233056 118-119 0.0882282996432818 120-121 0.08206004756242569 122-123 0.10778834720570749 124-125 0.10315101070154577 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3364000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.80367702712989 #Duplication Level Percentage of deduplicated Percentage of total 1 77.20663390348189 43.08414062701761 2 11.410797623744266 12.735289304347324 3 4.409370918395898 7.381773318689515 4 2.2473297067638445 5.016370445188965 5 1.3048825868814822 3.640862321833 6 0.8170309651466071 2.735599926012329 7 0.5404904711464282 2.1112948981667583 8 0.36837003456322004 1.6445121948190886 9 0.2838083499013328 1.4253794545946985 >10 1.317048257744821 12.679960072728862 >50 0.06547270582133999 2.4962300533517294 >100 0.026331597438304124 2.52569871971377 >500 8.293905594577963E-4 0.347277098366061 >1k 0.0014929030034642656 1.70966474563221 >5k 1.1058540766401967E-4 0.4659468195381979 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9688 0.2879904875148633 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5413 0.16090963139120093 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4490 0.13347205707491083 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4416 0.13127229488703923 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3610 0.1073127229488704 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.972651605231867E-5 0.0 0.0 0.0 0.0 3 2.972651605231867E-5 0.0 0.0 0.0 0.0 4 2.972651605231867E-5 0.0 0.0 0.0 0.0 5 5.945303210463734E-5 0.0 0.0 0.0 0.0 6 5.945303210463734E-5 0.0 0.0 0.0 0.0 7 8.9179548156956E-5 0.0 0.0 0.0 0.0 8 8.9179548156956E-5 0.0 0.0 0.0 0.0 9 8.9179548156956E-5 0.0 0.0 0.0 0.0 10-11 1.1890606420927467E-4 0.0 0.0 0.0 0.0 12-13 1.78359096313912E-4 0.0 0.0 0.0 0.0 14-15 2.3781212841854935E-4 0.0 0.0 0.0 0.0 16-17 2.5267538644470865E-4 2.972651605231867E-5 0.0 1.4863258026159334E-5 0.0 18-19 3.5671819262782397E-4 2.972651605231867E-5 0.0 4.4589774078478E-5 0.0 20-21 4.1617122473246133E-4 2.972651605231867E-5 0.0 1.1890606420927467E-4 0.0 22-23 4.4589774078478004E-4 2.972651605231867E-5 0.0 3.2699167657550536E-4 0.0 24-25 6.24256837098692E-4 2.972651605231867E-5 0.0 4.904875148632581E-4 0.0 26-27 6.24256837098692E-4 2.972651605231867E-5 0.0 7.13436385255648E-4 0.0 28-29 8.9179548156956E-4 2.972651605231867E-5 0.0 0.0010850178359096314 0.0 30-31 9.215219976218787E-4 2.972651605231867E-5 0.0 0.0028686087990487516 0.0 32-33 9.36385255648038E-4 2.972651605231867E-5 0.0 0.006361474435196195 0.0 34-35 0.001025564803804994 2.972651605231867E-5 0.0 0.0116230677764566 0.0 36-37 0.0010998810939357908 2.972651605231867E-5 0.0 0.01917360285374554 0.0 38-39 0.001248513674197384 2.972651605231867E-5 0.0 0.032684304399524376 0.0 40-41 0.0012633769322235432 2.972651605231867E-5 0.0 0.050817479191438766 0.0 42-43 0.001293103448275862 2.972651605231867E-5 0.0 0.06737514863258026 0.0 44-45 0.001501189060642093 2.972651605231867E-5 0.0 0.08647443519619501 0.0 46-47 0.00178359096313912 2.972651605231867E-5 0.0 0.11037455410225921 1.4863258026159334E-5 48-49 0.0018430439952437574 2.972651605231867E-5 0.0 0.13634066587395957 2.972651605231867E-5 50-51 0.0018430439952437574 2.972651605231867E-5 0.0 0.16709274673008323 2.972651605231867E-5 52-53 0.0018430439952437574 2.972651605231867E-5 0.0 0.19637336504161712 2.972651605231867E-5 54-55 0.0018727705112960762 2.972651605231867E-5 0.0 0.22899821640903684 2.972651605231867E-5 56-57 0.002051129607609988 2.972651605231867E-5 0.0 0.26908442330558857 2.972651605231867E-5 58-59 0.002080856123662307 2.972651605231867E-5 0.0 0.31624554102259217 2.972651605231867E-5 60-61 0.0021105826397146256 2.972651605231867E-5 0.0 0.37000594530321046 2.972651605231867E-5 62-63 0.002140309155766944 2.972651605231867E-5 0.0 0.4223692033293698 2.972651605231867E-5 64-65 0.002140309155766944 2.972651605231867E-5 0.0 0.47554994054696786 2.972651605231867E-5 66-67 0.002348394768133175 2.972651605231867E-5 0.0 0.5373662306777646 2.972651605231867E-5 68-69 0.002348394768133175 2.972651605231867E-5 0.0 0.613436385255648 2.972651605231867E-5 70-71 0.0024227110582639715 2.972651605231867E-5 0.0 0.7000297265160522 2.972651605231867E-5 72-73 0.002526753864447087 2.972651605231867E-5 0.0 0.7900564803804994 2.972651605231867E-5 74-75 0.002615933412604043 2.972651605231867E-5 0.0 0.8809304399524376 2.972651605231867E-5 76-77 0.002630796670630202 2.972651605231867E-5 0.0 0.9820451843043995 2.972651605231867E-5 78-79 0.0026605231866825207 2.972651605231867E-5 0.0 1.1063614744351962 2.972651605231867E-5 80-81 0.0033145065398335312 2.972651605231867E-5 0.0 1.2505945303210464 2.972651605231867E-5 82-83 0.0033888228299643283 2.972651605231867E-5 0.0 1.398796076099881 2.972651605231867E-5 84-85 0.003522592152199762 2.972651605231867E-5 0.0 1.551412009512485 2.972651605231867E-5 86-87 0.003730677764565993 2.972651605231867E-5 0.0 1.7160523186682521 2.972651605231867E-5 88-89 0.003819857312722949 2.972651605231867E-5 0.0 1.9028834720570749 2.972651605231867E-5 90-91 0.004295481569560047 2.972651605231867E-5 0.0 2.1168400713436384 2.972651605231867E-5 92-93 0.004399524375743163 2.972651605231867E-5 0.0 2.355023781212842 2.972651605231867E-5 94-95 0.004399524375743163 2.972651605231867E-5 0.0 2.588807966706302 4.4589774078478E-5 96-97 0.004399524375743163 2.972651605231867E-5 0.0 2.8228448275862066 5.945303210463734E-5 98-99 0.004607609988109393 2.972651605231867E-5 0.0 3.0885552913198575 5.945303210463734E-5 100-101 0.00490487514863258 2.972651605231867E-5 0.0 3.3957193816884663 5.945303210463734E-5 102-103 0.005142687277051129 4.4589774078478E-5 0.0 3.741230677764566 5.945303210463734E-5 104-105 0.00601961950059453 5.945303210463734E-5 0.0 4.08153983353151 5.945303210463734E-5 106-107 0.006510107015457788 5.945303210463734E-5 0.0 4.425208085612367 5.945303210463734E-5 108-109 0.00670332936979786 5.945303210463734E-5 0.0 4.787083828775268 5.945303210463734E-5 110-111 0.006747919143876338 5.945303210463734E-5 0.0 5.20108501783591 5.945303210463734E-5 112-113 0.006881688466111771 5.945303210463734E-5 0.0 5.655439952437574 5.945303210463734E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18575 0.0 33.158684 1 TACATGG 18670 0.0 32.35089 2 ACATGGG 19885 0.0 28.996288 3 CATGGGA 13810 0.0 24.153522 4 ATGGGAG 5275 0.0 22.133272 5 CATGGGG 6460 0.0 21.48372 4 GTTATCA 2415 0.0 20.718948 1 GTGTAGG 1805 0.0 20.130667 1 ATGGGAT 5165 0.0 19.952065 5 AGTACAT 14365 0.0 18.908272 2 GAGTACT 5695 0.0 18.59494 12-13 TACCTGG 3260 0.0 17.906103 2 GTACTTT 6055 0.0 17.588024 14-15 GTACTAG 925 0.0 17.387117 1 ACTATAC 1140 0.0 17.242558 3 GTACCTG 3680 0.0 17.157879 1 GTATATA 2495 0.0 16.950924 1 TAGCCCT 2545 0.0 16.8512 4 GAGTACA 20600 0.0 16.65562 1 GTATAGA 1395 0.0 16.653133 1 >>END_MODULE