FastQCFastQC Report
Thu 26 May 2016
SRR1364635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364000
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT120830.3591854934601664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113370.3370095124851368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93530.27803210463733646No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT65610.1950356718192628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64900.19292508917954815No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50320.14958382877526755No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45800.1361474435196195No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37160.11046373365041617No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA36990.10995838287752675No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG181250.030.3089871
TACATGG183250.029.088182
ACATGGG193550.026.5917573
GAGTACT145250.023.6788412-13
AGTACTT149400.023.65890712-13
GTACTTT151100.022.91974814-15
CATGGGA138900.022.5769214
ATGGGAG58300.020.5354295
AGGACCG9750.020.1597715
TACTTTT178150.019.90767514-15
ATGGGAT48650.019.5890525
CATGGGG63950.019.0752264
GGACCGA6950.018.8494136
ACTTTTT193350.018.71283516-17
AGAGTAC304850.018.43010510-11
GTATCAA434450.017.9933721
AGTACAT145300.017.8713912
GTGTAGC22850.017.7313271
TACCTGG32300.016.7793982
ATACAGT32100.016.3244136
Produced by FastQC (version 0.11.5)