##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364635_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3364000 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.338997027348395 31.0 30.0 33.0 26.0 34.0 2 29.54558947681332 31.0 30.0 34.0 25.0 34.0 3 29.547717895362663 31.0 30.0 34.0 25.0 34.0 4 33.18035671819263 35.0 33.0 37.0 27.0 37.0 5 32.6092372175981 35.0 33.0 37.0 25.0 37.0 6 32.610810939357904 35.0 33.0 37.0 25.0 37.0 7 32.39155172413793 35.0 33.0 37.0 23.0 37.0 8 32.32010136741974 35.0 33.0 37.0 23.0 37.0 9 33.57257104637336 38.0 33.0 39.0 22.0 39.0 10-11 33.361294292508916 37.0 33.0 39.0 18.5 39.0 12-13 33.228496432818076 37.0 33.0 39.0 17.5 39.0 14-15 34.158810493460166 38.0 33.0 41.0 17.0 41.0 16-17 34.08687841854935 38.0 33.0 41.0 17.0 41.0 18-19 34.14377363258026 38.5 33.0 41.0 17.5 41.0 20-21 34.159705410225925 39.0 33.0 41.0 18.0 41.0 22-23 34.141819560047566 39.0 33.0 41.0 18.0 41.0 24-25 34.12050074316291 39.0 33.0 41.0 18.0 41.0 26-27 34.00096001783591 38.5 33.0 41.0 17.5 41.0 28-29 33.77262068965517 38.0 33.0 41.0 16.0 41.0 30-31 33.6993757431629 38.0 33.0 40.5 16.0 41.0 32-33 33.50062247324614 38.0 32.0 40.0 15.5 41.0 34-35 33.34159557074911 38.0 32.0 40.0 14.0 41.0 36-37 33.154413198573124 38.0 32.0 40.0 9.0 41.0 38-39 33.006691290130796 38.0 32.0 40.0 9.0 41.0 40-41 32.82745749108204 38.0 31.5 40.0 8.0 41.0 42-43 32.63540755053508 38.0 31.0 40.0 4.5 41.0 44-45 32.40475609393579 38.0 31.0 40.0 2.0 41.0 46-47 32.11295749108204 38.0 30.5 40.0 2.0 41.0 48-49 31.823995838287754 38.0 30.0 40.0 2.0 41.0 50-51 31.61346001783591 37.5 30.0 40.0 2.0 41.0 52-53 31.445708234244947 37.0 30.0 40.0 2.0 41.0 54-55 31.222633472057076 37.0 29.5 40.0 2.0 41.0 56-57 30.990341706302022 37.0 29.0 40.0 2.0 41.0 58-59 30.791691587395956 36.0 29.0 40.0 2.0 41.0 60-61 30.543084869203327 36.0 28.0 40.0 2.0 41.0 62-63 30.28691973840666 35.5 28.0 39.0 2.0 41.0 64-65 30.009231866825207 35.0 28.0 39.0 2.0 41.0 66-67 29.715740933412604 35.0 28.0 39.0 2.0 40.5 68-69 29.3957959274673 35.0 28.0 38.5 2.0 40.0 70-71 29.11918668252081 35.0 28.0 37.5 2.0 40.0 72-73 28.95862618906064 35.0 28.0 37.0 2.0 39.5 74-75 28.680883323424496 34.0 27.0 37.0 2.0 39.0 76-77 28.38536281212842 34.0 26.5 36.0 2.0 39.0 78-79 28.079191290130794 34.0 26.0 36.0 2.0 38.5 80-81 27.802608055885848 34.0 26.0 35.5 2.0 37.0 82-83 27.548437871581452 34.0 26.0 35.0 2.0 37.0 84-85 27.53282357312723 34.0 26.0 35.0 2.0 36.5 86-87 27.406420332936978 34.0 26.0 35.0 2.0 36.0 88-89 27.265439803804995 34.0 26.0 35.0 2.0 36.0 90-91 27.10523275862069 34.0 26.0 35.0 2.0 36.0 92-93 26.976004310344827 34.0 26.0 35.0 2.0 35.0 94-95 26.892782401902497 34.0 25.0 35.0 2.0 35.0 96-97 26.809020659928656 34.0 25.0 35.0 2.0 35.0 98-99 26.724784780023782 34.0 25.0 35.0 2.0 35.0 100-101 26.627638525564805 34.0 25.0 35.0 2.0 35.0 102-103 25.44428329369798 31.5 22.0 34.0 2.0 35.0 104-105 26.350868311533887 33.5 24.5 35.0 2.0 35.0 106-107 26.51328775267539 34.0 25.0 35.0 2.0 35.0 108-109 26.533260255648038 34.0 25.0 35.0 2.0 35.0 110-111 26.484958234244946 34.0 25.0 35.0 2.0 35.0 112-113 26.421151605231866 34.0 25.0 35.0 2.0 35.0 114-115 26.328382580261593 34.0 24.5 35.0 2.0 35.0 116-117 26.201940101070157 34.0 24.0 35.0 2.0 35.0 118-119 26.1041348097503 34.0 24.0 35.0 2.0 35.0 120-121 25.96616468489893 34.0 23.5 35.0 2.0 35.0 122-123 25.77474583828775 34.0 21.5 35.0 2.0 35.0 124-125 24.955607164090367 33.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.0218064338726798 1101 2 0.9958302401835759 1101 3 0.8880402723597562 1101 4 0.8776733611322101 1101 5 1.126711187519156 1101 6 1.0816456976732738 1101 7 1.0517994246790323 1101 8 1.1942117768596034 1101 9 1.3116849103449795 1101 10-11 1.3423336122275416 1101 12-13 1.2023174699694081 1101 14-15 1.5015352860036018 1101 16-17 1.518231009688165 1101 18-19 1.5348008859876998 1101 20-21 1.6192426275784797 1101 22-23 1.5240931110834381 1101 24-25 1.4637857648128332 1101 26-27 1.6487958258849105 1101 28-29 1.7653603713948414 1101 30-31 1.702264191529153 1101 32-33 1.7125570798630676 1101 34-35 1.668645656185177 1101 36-37 1.7403958248089708 1101 38-39 1.9251171525303477 1101 40-41 1.9306319155079876 1101 42-43 1.9258366822600905 1101 44-45 1.812174124147468 1101 46-47 1.9167724705661442 1101 48-49 2.0442528032405534 1101 50-51 1.9922286038207275 1101 52-53 1.9159841344296389 1101 54-55 1.8621061424030145 1101 56-57 1.8673066936629255 1101 58-59 1.8759021134623737 1101 60-61 1.875802605076494 1101 62-63 1.8146871419798032 1101 64-65 1.8422505256002246 1101 66-67 1.9208728199791807 1101 68-69 1.8848611287111332 1101 70-71 1.9082647894178812 1101 72-73 1.8625172119484965 1101 74-75 1.734919923633246 1101 76-77 1.7540001670819265 1101 78-79 1.7436850453957327 1101 80-81 1.7104534105786655 1101 82-83 1.717362055931261 1101 84-85 1.7295683232673404 1101 86-87 1.6192045639433914 1101 88-89 1.625230153037709 1101 90-91 1.7277863737578159 1101 92-93 1.6957733586097277 1101 94-95 1.5890391487910414 1101 96-97 1.5883377447595315 1101 98-99 1.6851613615195475 1101 100-101 1.6798133837595408 1101 102-103 1.6904764125480725 1101 104-105 1.7080660995846522 1101 106-107 1.68604916113431 1101 108-109 1.6734557379967612 1101 110-111 1.7021638412520304 1101 112-113 1.6072892365494447 1101 114-115 1.5639561456599331 1101 116-117 1.6339959541174025 1101 118-119 1.7159386038774933 1101 120-121 1.7030597579074005 1101 122-123 1.7894672468471562 1101 124-125 1.6254442929346595 1102 1 0.6374289727729696 1102 2 -0.2667978115800622 1102 3 0.21685020386660625 1102 4 0.579129810108391 1102 5 0.9748012995663089 1102 6 0.9843096516611496 1102 7 0.9645212219679422 1102 8 0.9665726576051483 1102 9 1.1414436330789783 1102 10-11 1.2189339236685228 1102 12-13 1.1885742417225487 1102 14-15 1.338879267197811 1102 16-17 1.465412379312525 1102 18-19 1.3858202660292278 1102 20-21 1.3681816698676315 1102 22-23 1.3818691047710914 1102 24-25 1.2711540736047766 1102 26-27 1.2969161050482043 1102 28-29 1.4320429357712428 1102 30-31 1.3657670896605083 1102 32-33 1.2870817147253675 1102 34-35 1.211054878895311 1102 36-37 1.2721086980011762 1102 38-39 1.1701134385071512 1102 40-41 1.1516822606585748 1102 42-43 1.1676450610367652 1102 44-45 1.0400465258228095 1102 46-47 1.0958770255396502 1102 48-49 1.2151621199199454 1102 50-51 1.1520738230226755 1102 52-53 1.2344086726378443 1102 54-55 1.2526678623089893 1102 56-57 1.1442105034452794 1102 58-59 1.2849062331941283 1102 60-61 1.0011213547781743 1102 62-63 1.1382950917793302 1102 64-65 1.1841481378860372 1102 66-67 1.1085519582064727 1102 68-69 0.9544381700814739 1102 70-71 0.8873924265235082 1102 72-73 0.9524870000851031 1102 74-75 1.0413852325683806 1102 76-77 1.1031918449851332 1102 78-79 1.0506953387588176 1102 80-81 1.0110690211103837 1102 82-83 0.9217346097473076 1102 84-85 0.9948738277778695 1102 86-87 0.9121130170005252 1102 88-89 1.0723282181658647 1102 90-91 0.9985672657796769 1102 92-93 1.0607793920329485 1102 94-95 0.8976710458365318 1102 96-97 0.8793073732975465 1102 98-99 0.9009995822488719 1102 100-101 0.7367882522025759 1102 102-103 0.7219269175955638 1102 104-105 0.7145465107225455 1102 106-107 1.002836732431625 1102 108-109 1.0346220456909059 1102 110-111 0.9932279238137234 1102 112-113 1.0294487545915452 1102 114-115 1.1170074618262227 1102 116-117 1.0897032070528212 1102 118-119 1.0222975634736962 1102 120-121 1.0963075816986283 1102 122-123 1.0084409458949324 1102 124-125 0.8447910723246643 1103 1 0.3596094887353729 1103 2 -0.9764832028538883 1103 3 0.02766052705663924 1103 4 0.32856783689313573 1103 5 0.6765501197314734 1103 6 0.6601194650518494 1103 7 0.7069862931332764 1103 8 0.7488774281518644 1103 9 0.9276714326448356 1103 10-11 1.0849192975447366 1103 12-13 1.1171492142521373 1103 14-15 1.1656059423624043 1103 16-17 1.2714432864179912 1103 18-19 1.333625532540566 1103 20-21 1.3071384877798167 1103 22-23 1.2676235869305756 1103 24-25 1.2134881130765152 1103 26-27 1.2925521848922514 1103 28-29 1.438552807874153 1103 30-31 1.3491804937062355 1103 32-33 1.2877335377033248 1103 34-35 1.2755629718175143 1103 36-37 1.4206696046176859 1103 38-39 1.286525300267762 1103 40-41 1.3929715526363822 1103 42-43 1.3942012850801149 1103 44-45 1.3826103350737142 1103 46-47 1.2782913934661835 1103 48-49 1.3024822924709127 1103 50-51 1.3020147507361628 1103 52-53 1.3243934658355663 1103 54-55 1.3340771106373595 1103 56-57 1.3282813401567424 1103 58-59 1.2486167460143491 1103 60-61 1.2152583744344412 1103 62-63 1.3322256938769783 1103 64-65 1.2391292753108765 1103 66-67 1.2038245833470462 1103 68-69 1.1601642020271292 1103 70-71 1.2076842781108788 1103 72-73 1.2758019237876574 1103 74-75 1.1566290284621665 1103 76-77 1.1123721748423527 1103 78-79 1.059368996309363 1103 80-81 1.149280811840221 1103 82-83 1.1579822137852211 1103 84-85 1.1466250927056052 1103 86-87 1.1403700566848975 1103 88-89 1.1096048208782996 1103 90-91 1.1934866890582896 1103 92-93 1.1714375334903266 1103 94-95 1.1175085316654396 1103 96-97 1.1031058103930498 1103 98-99 1.1462506214624817 1103 100-101 1.156338847985534 1103 102-103 1.1739878903533274 1103 104-105 1.1640689102437491 1103 106-107 1.212128933866417 1103 108-109 1.1682137089368005 1103 110-111 1.1638865845462476 1103 112-113 1.1761206746550066 1103 114-115 1.121244121666507 1103 116-117 1.1058910792868772 1103 118-119 1.2476771365537118 1103 120-121 1.2503284959791863 1103 122-123 1.2404601340594041 1103 124-125 1.150458922711259 1104 1 0.4667716533452264 1104 2 -0.46830311524743706 1104 3 0.2655533006634485 1104 4 0.39974010516991854 1104 5 0.7527572197899346 1104 6 0.7488648063961989 1104 7 0.8563484133165105 1104 8 0.8485108474159304 1104 9 0.7801097129234762 1104 10-11 0.9332821187973721 1104 12-13 0.8988914400658246 1104 14-15 1.010636878202824 1104 16-17 1.1736197685563923 1104 18-19 1.0345417010190232 1104 20-21 1.0416617611394443 1104 22-23 1.0118820462958311 1104 24-25 1.0136143255715453 1104 26-27 0.8537862470644058 1104 28-29 1.2194392267407252 1104 30-31 1.087741222329484 1104 32-33 1.13143839781781 1104 34-35 1.096813831208145 1104 36-37 1.0805369314830529 1104 38-39 1.1671712064346949 1104 40-41 1.276775326987817 1104 42-43 1.158999388981286 1104 44-45 1.1416828165827155 1104 46-47 1.166991096959059 1104 48-49 1.225895988204563 1104 50-51 1.1904907654171737 1104 52-53 1.0168227385557849 1104 54-55 1.237143403089199 1104 56-57 1.287704767520495 1104 58-59 1.2536521889676422 1104 60-61 1.2972263225946463 1104 62-63 1.1758392671762863 1104 64-65 1.2887561328142176 1104 66-67 1.2340700776509834 1104 68-69 1.217600277087449 1104 70-71 1.2198880974000978 1104 72-73 1.1368605019202285 1104 74-75 1.1960864903064454 1104 76-77 1.1856668199011011 1104 78-79 1.1917431569068206 1104 80-81 1.078939459157418 1104 82-83 1.120680687600796 1104 84-85 1.1216397302658372 1104 86-87 1.0872049044459402 1104 88-89 1.1115782384688622 1104 90-91 1.1442169224625047 1104 92-93 1.1349558106886555 1104 94-95 1.125437427737257 1104 96-97 1.1176374712496937 1104 98-99 1.096958630444263 1104 100-101 1.1480329701480692 1104 102-103 1.2063674848220707 1104 104-105 1.1033874583355647 1104 106-107 1.1237570739411709 1104 108-109 1.0560241577267888 1104 110-111 0.9469932787756434 1104 112-113 1.026644320940438 1104 114-115 1.090462796537544 1104 116-117 1.0712710963530085 1104 118-119 0.9628848310043523 1104 120-121 1.0179437676688607 1104 122-123 1.0527850542264972 1104 124-125 1.000114649739345 1105 1 0.7516032599846945 1105 2 0.4381751537747789 1105 3 0.6308314389013923 1105 4 0.5132844838061743 1105 5 0.7846063682503086 1105 6 0.6502912889491412 1105 7 0.721471320549341 1105 8 0.8059432634660126 1105 9 0.8076572265947775 1105 10-11 0.9027645926860757 1105 12-13 0.9374335088876791 1105 14-15 0.923148120614691 1105 16-17 1.094514848178278 1105 18-19 1.0600836214962754 1105 20-21 1.1054856631849432 1105 22-23 0.9903893488242801 1105 24-25 1.0451515235543454 1105 26-27 1.1183967198202893 1105 28-29 1.1829949105555428 1105 30-31 1.1178395745136243 1105 32-33 1.0656263875863417 1105 34-35 1.0319202591347008 1105 36-37 1.084191782373054 1105 38-39 1.1419129196886004 1105 40-41 1.024250904287186 1105 42-43 1.0779129778202616 1105 44-45 1.1379472118553764 1105 46-47 1.1075024367848343 1105 48-49 1.026078266275519 1105 50-51 1.006132946903449 1105 52-53 1.0116730719079108 1105 54-55 1.0732408382339784 1105 56-57 0.9983677315977211 1105 58-59 0.9612020684053775 1105 60-61 0.9593155755447142 1105 62-63 1.0462185623730065 1105 64-65 1.0749484676162204 1105 66-67 1.0444167585552862 1105 68-69 0.9627620587835892 1105 70-71 1.0246556439912773 1105 72-73 0.9601358668027586 1105 74-75 0.9745312269926245 1105 76-77 0.9798704446741908 1105 78-79 0.9721985619227347 1105 80-81 0.7464294634714648 1105 82-83 0.7720905818127335 1105 84-85 0.6661737589250372 1105 86-87 0.7693793027500462 1105 88-89 0.7904679659208078 1105 90-91 0.7738114804536984 1105 92-93 0.700049985121538 1105 94-95 0.8758878236121888 1105 96-97 0.8926572983521943 1105 98-99 0.8674156236369086 1105 100-101 0.9567224309527447 1105 102-103 0.6339761260816381 1105 104-105 0.4786382919848826 1105 106-107 0.822493738170337 1105 108-109 0.8828042355632277 1105 110-111 0.8238432464310677 1105 112-113 0.5914676839814241 1105 114-115 0.7273274953571125 1105 116-117 0.4887262382509334 1105 118-119 0.4641453297205409 1105 120-121 0.3747797315364778 1105 122-123 0.47655848897933595 1105 124-125 0.3419503058708173 1106 1 0.50498596325005 1106 2 0.5131062657454954 1106 3 0.6367167220470797 1106 4 0.42791898381832283 1106 5 0.5764459213733844 1106 6 0.6113648077552227 1106 7 0.6286062907614749 1106 8 0.6851649165199802 1106 9 0.7234678662927934 1106 10-11 0.8512499374446563 1106 12-13 0.7255247009580046 1106 14-15 0.8560062818576739 1106 16-17 1.0463090964845208 1106 18-19 1.0104429779641606 1106 20-21 1.058436948192103 1106 22-23 0.9811629980353302 1106 24-25 1.0458194882999408 1106 26-27 1.0559105894758787 1106 28-29 1.103037563344948 1106 30-31 0.9631268983343446 1106 32-33 1.0345494206470462 1106 34-35 1.067862214350619 1106 36-37 1.1011436823719265 1106 38-39 1.122529769466702 1106 40-41 1.1251849105289438 1106 42-43 1.0963729118771113 1106 44-45 1.0808223031409838 1106 46-47 1.1143587912941513 1106 48-49 1.1840795168724227 1106 50-51 1.3491830890580871 1106 52-53 1.3116458763654215 1106 54-55 1.3489105942176067 1106 56-57 1.148895272522175 1106 58-59 1.116056515388351 1106 60-61 1.2307130511216826 1106 62-63 1.2594975963156898 1106 64-65 1.2014353018034463 1106 66-67 1.1609958429227412 1106 68-69 1.1851324431647896 1106 70-71 1.3226163443710384 1106 72-73 1.3223509408953298 1106 74-75 1.301841326973559 1106 76-77 1.2358393011577853 1106 78-79 1.109897106797721 1106 80-81 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0.8381596214936593 2203 90-91 0.7407625751637923 2203 92-93 0.8572375840443875 2203 94-95 0.8349977239196384 2203 96-97 0.7783192846580214 2203 98-99 0.7381893141693681 2203 100-101 0.8500163830534859 2203 102-103 0.8673966560696265 2203 104-105 0.8479419618329835 2203 106-107 0.8509157641970759 2203 108-109 0.7972888176914168 2203 110-111 0.6889346999302859 2203 112-113 0.6696866606909175 2203 114-115 0.7693461286281469 2203 116-117 0.7721860121539486 2203 118-119 0.7739294231427358 2203 120-121 0.7728284850543368 2203 122-123 0.7709366355390976 2203 124-125 0.763992051856448 2204 1 0.3248657952716947 2204 2 0.4595988623030678 2204 3 0.5579049702900178 2204 4 0.5195960327916822 2204 5 0.4115734423887858 2204 6 0.40795360915346635 2204 7 0.5369121749826036 2204 8 0.6006130291733314 2204 9 0.6084921896708053 2204 10-11 0.6077435830984186 2204 12-13 0.6462431173591625 2204 14-15 0.644330403179552 2204 16-17 0.6986808824614172 2204 18-19 0.7613517853580447 2204 20-21 0.7754551786852133 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22-23 0.709527548999894 2205 24-25 0.7137742471665405 2205 26-27 0.8110998193504528 2205 28-29 0.9285272081072478 2205 30-31 0.7873316191213178 2205 32-33 0.8073922591938683 2205 34-35 0.8666274144739603 2205 36-37 0.8261304707012016 2205 38-39 0.8292081014667758 2205 40-41 0.9659949580206302 2205 42-43 0.927166060197969 2205 44-45 0.8663848846117972 2205 46-47 0.9050319105550741 2205 48-49 0.959045518514813 2205 50-51 0.9159016519402599 2205 52-53 0.8756562686992062 2205 54-55 0.8012059224684833 2205 56-57 0.9199866399073784 2205 58-59 0.813569486918098 2205 60-61 0.9178285709624134 2205 62-63 0.779682546824013 2205 64-65 0.9312767502656065 2205 66-67 0.8566314498819878 2205 68-69 0.822723024360279 2205 70-71 0.8834113891006261 2205 72-73 0.8831182027321383 2205 74-75 0.840678207656584 2205 76-77 0.8373321059281977 2205 78-79 0.8247965290702233 2205 80-81 0.8156185008097978 2205 82-83 0.7757484007778537 2205 84-85 0.8455640444442416 2205 86-87 0.8299737102624505 2205 88-89 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2216 106-107 -2.911436452865651 2216 108-109 -3.055535019324843 2216 110-111 -2.954350101472201 2216 112-113 -3.0203283102463736 2216 114-115 -3.084373919773622 2216 116-117 -3.1331537903063875 2216 118-119 -3.2831220323492225 2216 120-121 -3.2585854328649084 2216 122-123 -3.2194149157720986 2216 124-125 -3.3220771089754564 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 145830.0 3 45851.0 4 20913.0 5 16452.0 6 17771.0 7 20834.0 8 23522.0 9 24897.0 10 26997.0 11 30124.0 12 33712.0 13 37881.0 14 41171.0 15 33328.0 16 22015.0 17 18646.0 18 16736.0 19 15769.0 20 15557.0 21 16259.0 22 18979.0 23 25066.0 24 34344.0 25 44674.0 26 51721.0 27 58443.0 28 68215.0 29 79527.0 30 92371.0 31 109012.0 32 132109.0 33 163053.0 34 209331.0 35 284559.0 36 447358.0 37 669141.0 38 250868.0 39 964.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58990794307045 19.54825181065124 13.957317707946046 23.90452253833227 2 15.296157731837312 21.55464364804257 38.389957673053196 24.75924094706692 3 20.268162901307967 25.38549346016647 29.060196195005943 25.286147443519617 4 12.744745666068228 17.31075494084424 36.657513183958244 33.28698620912928 5 12.979924503167506 39.54877336231492 33.40816608708451 14.063136047433064 6 31.31031899619202 37.45002214632029 16.78545417792562 14.45420467956207 7 27.864417360285376 33.553507728894175 20.310701545778834 18.27137336504162 8 25.9724435196195 35.624910820451845 18.92125445897741 19.481391200951247 9 26.153860788486526 16.77490621996683 19.85152151210958 37.21971147943706 10-11 24.832713411881997 27.361683604642845 26.47667639416148 21.328926589313678 12-13 26.451442680020985 24.490346210503002 27.358195798977615 21.700015310498394 14-15 22.908358776016197 25.695365616555232 26.500652541642268 24.895623065786303 16-17 21.71043493855826 28.853778569433757 26.916528045906794 22.519258446101183 18-19 21.584995703770797 27.724166776035585 29.344620305044906 21.34621721514871 20-21 22.91363086031711 26.180328197825443 29.19610931206888 21.709931629788574 22-23 22.10973578876658 26.306618577539552 29.13039555204809 22.453250081645777 24-25 22.27917657550535 26.368593935790724 29.37387039239001 21.978359096313913 26-27 22.17430142687277 26.108234244946495 29.851114744351964 21.866349583828775 28-29 21.630736731094345 26.867873116245374 29.660446457820832 21.840943694839446 30-31 21.920407253269918 26.341186087990486 29.507223543400713 22.231183115338883 32-33 21.843815846425326 26.397356273455003 29.55058254020348 22.208245339916193 34-35 22.416319857312722 26.125743162901312 29.4634661117717 21.994470868014268 36-37 22.272944006439964 25.97965394706411 29.70677117735644 22.040630869139484 38-39 22.177274078478 26.267568370986922 29.6259066587396 21.929250891795483 40-41 22.157669441141497 26.48671224732461 29.37804696789536 21.977571343638523 42-43 21.93776894939935 26.605937543615678 28.8523482939963 22.603945212988677 44-45 22.175639120095127 26.373721759809747 28.675118906064213 22.775520214030916 46-47 22.554457523458694 26.829623561012816 28.188349101277705 22.42756981425078 48-49 22.60814751992647 26.812347707025552 27.966511820922342 22.612992952125644 50-51 22.319106310059897 27.08630878462467 28.182047752583948 22.412537152731485 52-53 22.695974060622813 27.391919881034394 27.459845020693386 22.452261037649404 54-55 22.220109988109392 26.980350772889416 27.826263376932225 22.973275862068967 56-57 22.585422080986454 26.949329770069923 27.592367026040016 22.872881122903603 58-59 22.992746730083237 26.710374554102263 27.607357312722947 22.689521403091558 60-61 22.64241973840666 26.809036860879903 28.19659631391201 22.351947086801427 62-63 22.545659928656363 26.88996730083234 28.052095719381686 22.512277051129608 64-65 22.250054659948237 27.214233257297423 27.93122611739277 22.60448596536157 66-67 22.393177764565994 26.903938763376935 27.705856123662308 22.99702734839477 68-69 22.22061698272049 26.92573745891776 27.874493457757232 22.979152100604523 70-71 22.492152199762188 26.74677467300832 27.725653983353148 23.035419143876336 72-73 22.59445140715444 26.54673728994944 27.92150423073813 22.937307072157985 74-75 22.224355983523335 27.378761937193 27.558210932312814 22.838671146970853 76-77 22.545879366287306 26.674658180699378 27.718420266439953 23.061042186573363 78-79 22.52264732129321 26.756048055068206 27.828693087401984 22.892611536236608 80-81 22.56496228245825 26.95539353712538 27.632543508349706 22.847100672066663 82-83 22.56128791952673 26.873581115808893 27.739321563413068 22.82580940125131 84-85 22.433663753664536 26.848202644591346 27.723366660741178 22.994766941002936 86-87 22.33263525253531 26.96975462137911 27.724142646295984 22.973467479789594 88-89 22.40711950059453 26.885404280618314 27.43082639714625 23.276649821640905 90-91 22.391527942925087 26.706391200951245 27.65564803804994 23.24643281807372 92-93 22.458317540300104 27.013503972412355 27.717955006577334 22.810223480710203 94-95 22.28252371249745 26.904847974248465 27.650945140639948 23.16168317261414 96-97 21.90681808047327 26.8646491371494 27.88015220654904 23.348380575828294 98-99 22.209618085746776 27.01457353279974 27.26432271345655 23.511485667996936 100-101 22.018489075387194 26.83250958349565 27.615479431918054 23.533521909199102 102-103 22.313043429147445 26.46162087267332 27.713286859840668 23.512048838338565 104-105 22.470957859280833 26.413328692020876 27.616170792371324 23.499542656326962 106-107 22.078384012545012 26.762978845160546 27.947144486847435 23.211492655447007 108-109 21.865962318670398 26.632484781282262 27.702235596378276 23.799317303669064 110-111 21.651306139053464 27.054713026640027 27.531410343017846 23.762570491288663 112-113 22.06715739178554 26.60913183925901 27.40074974728246 23.922961021672993 114-115 21.815611818108852 26.666324810715846 27.550616975674718 23.96744639550058 116-117 21.837264380205752 26.651475186737482 27.532820820304245 23.97843961275252 118-119 21.67887381052993 26.457027915583055 27.936793268499745 23.927305005387275 120-121 21.416641446228144 27.089025274149638 27.541581021228573 23.95275225839365 122-123 21.43083702588183 26.601747571776546 27.391065112714635 24.576350289626998 124-125 21.44524933593603 26.503372304931812 27.5816894179766 24.469688941155557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 488.0 1 380.5 2 390.5 3 630.5 4 556.5 5 488.0 6 1141.0 7 2443.5 8 3728.5 9 4220.0 10 3498.0 11 2656.0 12 2625.0 13 2889.5 14 3264.5 15 3605.0 16 3943.5 17 4349.5 18 4778.5 19 5251.5 20 5868.0 21 6695.0 22 7756.0 23 9261.5 24 11167.5 25 13923.0 26 17466.5 27 21852.5 28 26374.5 29 31495.0 30 37881.5 31 43796.5 32 49648.5 33 55601.0 34 61954.0 35 68830.0 36 76526.0 37 83648.0 38 88876.5 39 92588.0 40 96302.0 41 100792.5 42 105405.0 43 111161.5 44 116192.5 45 119582.0 46 123066.0 47 136506.5 48 167345.0 49 167487.0 50 139325.5 51 123526.0 52 113649.5 53 108679.0 54 105070.0 55 99448.0 56 90725.0 57 82229.0 58 75923.0 59 68182.0 60 57242.0 61 45590.5 62 35915.5 63 27826.0 64 20663.5 65 15529.0 66 11553.5 67 8628.5 68 6651.5 69 4993.5 70 3689.0 71 2941.0 72 2374.0 73 2119.5 74 1719.0 75 1227.0 76 878.0 77 530.5 78 343.0 79 230.5 80 144.0 81 91.0 82 69.0 83 49.5 84 35.5 85 26.5 86 16.5 87 9.0 88 7.0 89 5.0 90 3.0 91 2.0 92 1.5 93 2.0 94 1.0 95 0.5 96 0.5 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0579667063020214 2 0.07574316290130798 3 0.0 4 0.007520808561236623 5 0.02809155766944114 6 2.9726516052318666E-4 7 0.0 8 0.0 9 3.2699167657550536E-4 10-11 0.05590071343638525 12-13 0.008769322235434007 14-15 0.006837098692033293 16-17 0.050802615933412607 18-19 1.0404280618311535E-4 20-21 0.009735434007134364 22-23 0.011429845422116527 24-25 0.0 26-27 0.0 28-29 0.047547562425683705 30-31 0.0 32-33 0.003329369797859691 34-35 0.0 36-37 1.78359096313912E-4 38-39 0.0 40-41 0.0 42-43 0.002987514863258026 44-45 0.0 46-47 5.945303210463733E-5 48-49 2.080856123662307E-4 50-51 4.310344827586207E-4 52-53 7.431629013079666E-5 54-55 0.0 56-57 0.0012633769322235435 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.79667063020214E-4 66-67 0.0 68-69 4.7562425683709864E-4 70-71 0.0 72-73 0.001426872770511296 74-75 0.0027199762187871583 76-77 4.4589774078478004E-4 78-79 7.728894173602853E-4 80-81 0.001426872770511296 82-83 2.9726516052318665E-5 84-85 5.945303210463733E-5 86-87 4.4589774078478004E-4 88-89 0.0 90-91 0.0 92-93 0.005202140309155767 94-95 0.004815695600475624 96-97 0.0044589774078478 98-99 7.431629013079667E-4 100-101 4.01307966706302E-4 102-103 1.0404280618311535E-4 104-105 2.67538644470868E-4 106-107 0.00334423305588585 108-109 0.003938763376932224 110-111 6.093935790725327E-4 112-113 0.0019768133174791914 114-115 2.9726516052318666E-4 116-117 0.0015755053507728895 118-119 0.0016200951248513675 120-121 0.009690844233055886 122-123 5.35077288941736E-4 124-125 0.0016200951248513675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3364000.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.63514408193372 #Duplication Level Percentage of deduplicated Percentage of total 1 73.30478981092507 35.65189014350207 2 12.545624067859665 12.203164682762601 3 5.229118399805502 7.629567803880939 4 2.762599406026223 5.374376806109995 5 1.6428228852384985 3.994946386233621 6 1.0055542543480842 2.93431656254523 7 0.7151535141239171 2.434711594008255 8 0.4898795258324233 1.9060289057319417 9 0.37583799319363564 1.6451041455393562 >10 1.777177866945651 15.068926095106622 >50 0.10112187984541837 3.3417876337876047 >100 0.04607191089991502 3.854075158783733 >500 0.0019703118649906978 0.6407492222069668 >1k 0.0019087396167089127 1.7974337769084727 >5k 2.4628898280115E-4 0.8215916082711769 >10k+ 1.23144491400575E-4 0.7013294746213357 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 12083 0.3591854934601664 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11337 0.3370095124851368 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9353 0.27803210463733646 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6561 0.1950356718192628 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6490 0.19292508917954815 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5032 0.14958382877526755 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4580 0.1361474435196195 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3716 0.11046373365041617 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3699 0.10995838287752675 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 2.972651605231867E-5 0.0 0.0 0.0 0.0 3 2.972651605231867E-5 0.0 0.0 0.0 0.0 4 2.972651605231867E-5 0.0 0.0 0.0 0.0 5 5.945303210463734E-5 0.0 0.0 0.0 0.0 6 8.9179548156956E-5 0.0 0.0 0.0 0.0 7 1.78359096313912E-4 0.0 0.0 0.0 0.0 8 1.78359096313912E-4 0.0 0.0 0.0 0.0 9 1.78359096313912E-4 0.0 0.0 0.0 0.0 10-11 2.3781212841854935E-4 0.0 0.0 0.0 0.0 12-13 2.5267538644470865E-4 0.0 0.0 0.0 0.0 14-15 4.310344827586207E-4 0.0 0.0 0.0 0.0 16-17 4.756242568370987E-4 0.0 0.0 0.0 0.0 18-19 5.796670630202141E-4 0.0 0.0 4.4589774078478E-5 0.0 20-21 6.539833531510107E-4 0.0 0.0 1.1890606420927467E-4 0.0 22-23 7.13436385255648E-4 0.0 0.0 3.56718192627824E-4 0.0 24-25 8.026159334126041E-4 0.0 0.0 5.202140309155767E-4 0.0 26-27 8.174791914387634E-4 0.0 0.0 7.431629013079667E-4 0.0 28-29 0.0010404280618311534 0.0 0.0 0.0011890606420927466 0.0 30-31 0.0011890606420927466 0.0 0.0 0.003032104637336504 0.0 32-33 0.001248513674197384 0.0 0.0 0.006584423305588585 0.0 34-35 0.0013525564803804995 0.0 0.0 0.012158145065398335 0.0 36-37 0.0014565992865636147 0.0 0.0 0.02028834720570749 0.0 38-39 0.0015457788347205707 0.0 0.0 0.03394768133174792 0.0 40-41 0.00156064209274673 0.0 0.0 0.052422711058263974 0.0 42-43 0.0016646848989298455 0.0 0.0 0.06969381688466111 0.0 44-45 0.0019470868014268726 0.0 0.0 0.08923900118906064 0.0 46-47 0.002214625445897741 0.0 0.0 0.11284185493460167 0.0 48-49 0.0022294887039239 0.0 0.0 0.13894173602853746 0.0 50-51 0.0022294887039239 0.0 0.0 0.1703180737217598 0.0 52-53 0.0022294887039239 0.0 0.0 0.20008917954815697 0.0 54-55 0.002348394768133175 0.0 0.0 0.23268430439952437 0.0 56-57 0.002407847800237812 0.0 0.0 0.27271105826397146 0.0 58-59 0.002437574316290131 0.0 0.0 0.31978299643281805 0.0 60-61 0.002437574316290131 0.0 0.0 0.37378121284185495 0.0 62-63 0.002437574316290131 0.0 0.0 0.42650118906064205 0.0 64-65 0.0024673008323424493 0.0 0.0 0.47959274673008323 0.0 66-67 0.002705112960760999 0.0 0.0 0.5410671819262782 0.0 68-69 0.002794292508917955 0.0 0.0 0.6165279429250892 0.0 70-71 0.002853745541022592 0.0 0.0 0.7039387633769323 0.0 72-73 0.002972651605231867 0.0 0.0 0.7935047562425683 0.0 74-75 0.0030766944114149824 0.0 0.0 0.8833382877526754 0.0 76-77 0.0030915576694411414 0.0 0.0 0.9835017835909632 0.0 78-79 0.003106420927467301 0.0 0.0 1.107104637336504 0.0 80-81 0.0034482758620689655 0.0 0.0 1.2512782401902496 0.0 82-83 0.003522592152199762 0.0 0.0 1.3988109393579071 0.0 84-85 0.0035969084423305587 0.0 0.0 1.5499554102259214 0.0 86-87 0.003700951248513674 0.0 0.0 1.713153983353151 0.0 88-89 0.0037604042806183118 0.0 0.0 1.8986623067776456 0.0 90-91 0.004131985731272295 0.0 0.0 2.1103596908442332 0.0 92-93 0.0041765755053507735 0.0 0.0 2.34589774078478 0.0 94-95 0.0041914387633769324 0.0 0.0 2.5786860879904876 0.0 96-97 0.0041914387633769324 0.0 0.0 2.811593341260404 0.0 98-99 0.004518430439952438 0.0 0.0 3.076501189060642 0.0 100-101 0.004860285374554102 0.0 0.0 3.382282996432818 1.4863258026159334E-5 102-103 0.005112960760998811 0.0 0.0 3.7270362663495837 2.972651605231867E-5 104-105 0.0056629013079667065 0.0 0.0 4.0664833531510105 2.972651605231867E-5 106-107 0.0058709869203329365 0.0 0.0 4.4089030915576695 2.972651605231867E-5 108-109 0.005960166468489893 0.0 1.4863258026159334E-5 4.771819262782402 2.972651605231867E-5 110-111 0.006108799048751486 0.0 2.972651605231867E-5 5.187782401902497 2.972651605231867E-5 112-113 0.006197978596908443 0.0 2.972651605231867E-5 5.6435344827586205 2.972651605231867E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 18125 0.0 30.308987 1 TACATGG 18325 0.0 29.08818 2 ACATGGG 19355 0.0 26.591757 3 GAGTACT 14525 0.0 23.67884 12-13 AGTACTT 14940 0.0 23.658907 12-13 GTACTTT 15110 0.0 22.919748 14-15 CATGGGA 13890 0.0 22.576921 4 ATGGGAG 5830 0.0 20.535429 5 AGGACCG 975 0.0 20.159771 5 TACTTTT 17815 0.0 19.907675 14-15 ATGGGAT 4865 0.0 19.589052 5 CATGGGG 6395 0.0 19.075226 4 GGACCGA 695 0.0 18.849413 6 ACTTTTT 19335 0.0 18.712835 16-17 AGAGTAC 30485 0.0 18.430105 10-11 GTATCAA 43445 0.0 17.993372 1 AGTACAT 14530 0.0 17.871391 2 GTGTAGC 2285 0.0 17.731327 1 TACCTGG 3230 0.0 16.779398 2 ATACAGT 3210 0.0 16.324413 6 >>END_MODULE