FastQCFastQC Report
Thu 26 May 2016
SRR1364634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239199
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28310.1264291382766784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65900.038.6820641
GTACATG98350.033.065081
TACATGG98400.032.1987232
ATCAACG78150.031.549573
TACCTGG22300.031.5136872
TCAACGC78800.030.8358574
GGTATCA45900.030.7529621
CAACGCA80350.030.5388625
GTACCTG23800.030.2802661
ACATGGG99200.030.2580533
AACGCAG81550.029.8503326
TATCAAC84200.029.7777652
CGGTACG808.764428E-626.02523878-79
ACGCAGA92900.025.5581427
CGCAGAG90900.024.2831138
CATGGGA59600.024.2818684
CATGGGG46850.023.1357674
GTATACG1300.002040862322.9075131
ACGCATA3205.020411E-1022.3153327
GTATAGA8950.021.2950291
Produced by FastQC (version 0.11.5)