FastQCFastQC Report
Thu 26 May 2016
SRR1364634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2239199
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66130.295328820707762No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57620.2573241592194352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39530.1765363417900776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54500.052.2442551
GTATCAA87750.043.173491
GTACCGT1902.910383E-1134.4693156
ATCAACG110350.034.1837233
TCAACGC112150.033.688134
TATCAAC113200.033.134312
CAACGCA112850.032.9821365
AACGCAG117100.032.0314946
GTACATG99250.031.2690261
TACATGG98850.030.0543772
TTTACGC1203.698408E-529.7490043
ACATGGG103800.027.800133
ACGCAGA136150.027.2447837
CGCAGAG137850.026.86568
GAGTACT85650.024.4365612-13
GTACTTT88650.023.47526614-15
AGTACTT90250.023.38897112-13
GCAGAGT152150.023.3622889
TACCTGG21700.023.0464152
CATGGGA63150.022.988944
Produced by FastQC (version 0.11.5)