##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364634_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2239199 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.636227954728454 31.0 30.0 33.0 26.0 34.0 2 29.673985652905348 31.0 30.0 34.0 26.0 34.0 3 29.69313803730709 31.0 30.0 34.0 26.0 34.0 4 33.38482019686504 35.0 33.0 37.0 28.0 37.0 5 32.82949661910353 35.0 33.0 37.0 26.0 37.0 6 32.87400539210673 35.0 33.0 37.0 26.0 37.0 7 32.68279683940552 35.0 33.0 37.0 25.0 37.0 8 32.62101045954379 35.0 33.0 37.0 25.0 37.0 9 33.8949963804021 38.0 33.0 39.0 25.0 39.0 10-11 33.70223280735656 37.0 33.0 39.0 22.5 39.0 12-13 33.56869197422829 37.0 33.0 39.0 22.0 39.0 14-15 34.5667812463296 38.0 33.0 41.0 22.0 41.0 16-17 34.49462798974098 38.0 33.0 41.0 20.5 41.0 18-19 34.53015229999656 38.5 33.5 41.0 21.5 41.0 20-21 34.55066231272879 39.0 33.5 41.0 22.0 41.0 22-23 34.54175935233983 39.0 34.0 41.0 22.0 41.0 24-25 34.53881678225115 39.0 33.5 41.0 22.0 41.0 26-27 34.447650476799964 39.0 33.0 41.0 21.5 41.0 28-29 34.21417078160539 39.0 33.0 41.0 19.0 41.0 30-31 34.14604039212236 38.0 33.0 41.0 19.0 41.0 32-33 33.980223955083936 38.0 33.0 41.0 18.5 41.0 34-35 33.850184373965874 38.0 33.0 40.0 17.5 41.0 36-37 33.69684829262607 38.0 33.0 40.0 16.0 41.0 38-39 33.56718317576955 38.0 33.0 40.0 15.5 41.0 40-41 33.40145315356071 38.0 32.0 40.0 15.0 41.0 42-43 33.23401872723237 38.0 32.0 40.0 11.0 41.0 44-45 33.03823197491603 38.0 32.0 40.0 9.0 41.0 46-47 32.79480050678836 38.0 31.5 40.0 7.5 41.0 48-49 32.55121987817965 38.0 31.0 40.0 2.0 41.0 50-51 32.35745460765211 38.0 31.0 40.0 2.0 41.0 52-53 32.180827161855646 38.0 31.0 40.0 2.0 41.0 54-55 31.960170132266043 37.0 30.5 40.0 2.0 41.0 56-57 31.744960586352533 37.0 30.0 40.0 2.0 41.0 58-59 31.561710683150537 37.0 30.0 40.0 2.0 41.0 60-61 31.33920477813718 36.0 30.0 40.0 2.0 41.0 62-63 31.06229057801473 36.0 29.0 39.5 2.0 41.0 64-65 30.791754104927698 35.5 29.0 39.0 2.0 41.0 66-67 30.50933280159557 35.0 29.0 39.0 2.0 41.0 68-69 30.1988840652394 35.0 29.0 39.0 2.0 40.0 70-71 29.937223533951205 35.0 29.0 38.0 2.0 40.0 72-73 29.782912550425397 35.0 29.0 37.0 2.0 40.0 74-75 29.500060066121858 35.0 29.0 37.0 2.0 39.0 76-77 29.206382058941614 34.5 29.0 36.5 2.0 39.0 78-79 28.901814443468403 34.0 28.0 36.0 2.0 39.0 80-81 28.625881174473548 34.0 28.0 36.0 2.0 37.5 82-83 28.372870164733015 34.0 28.0 35.0 2.0 37.0 84-85 28.351662581128338 34.0 28.0 35.0 2.0 37.0 86-87 28.20597454714833 34.0 28.0 35.0 2.0 36.0 88-89 28.061392935598846 34.0 28.0 35.0 2.0 36.0 90-91 27.906951325005057 34.0 28.0 35.0 2.0 36.0 92-93 27.772136822140418 34.0 27.0 35.0 2.0 35.5 94-95 27.681274420004655 34.0 27.0 35.0 2.0 35.0 96-97 27.598139334646007 34.0 27.0 35.0 2.0 35.0 98-99 27.512227140151456 34.0 27.0 35.0 2.0 35.0 100-101 27.41609566635212 34.0 27.0 35.0 2.0 35.0 102-103 26.229040161236227 32.0 24.5 34.0 2.0 35.0 104-105 27.166091088822387 34.0 26.5 35.0 2.0 35.0 106-107 27.33827855407224 34.0 27.0 35.0 2.0 35.0 108-109 27.354026372823498 34.0 27.0 35.0 2.0 35.0 110-111 27.295004597626203 34.0 27.0 35.0 2.0 35.0 112-113 27.244473581847792 34.0 27.0 35.0 2.0 35.0 114-115 27.16677325239963 34.0 27.0 35.0 2.0 35.0 116-117 27.05754557768202 34.0 27.0 35.0 2.0 35.0 118-119 26.957082197696586 34.0 26.0 35.0 2.0 35.0 120-121 26.823125814186234 34.0 25.5 35.0 2.0 35.0 122-123 26.641026768947288 34.0 25.0 35.0 2.0 35.0 124-125 25.83919517648945 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6237583284771162 1101 2 0.6348888200927192 1101 3 0.48495090398682805 1101 4 0.4810111936769914 1101 5 0.729767759885199 1101 6 0.6718032257875848 1101 7 0.756672776610749 1101 8 0.88469834103973 1101 9 0.9005995181508624 1101 10-11 0.9395726906348614 1101 12-13 0.9163974711579428 1101 14-15 1.0044752259004497 1101 16-17 1.1074884120856012 1101 18-19 1.158326061527589 1101 20-21 1.2508241812632406 1101 22-23 1.058445605590272 1101 24-25 0.9184898930061962 1101 26-27 1.0705689956555346 1101 28-29 1.2320284460344908 1101 30-31 1.2454362070067404 1101 32-33 1.356843114677659 1101 34-35 1.2500268774251069 1101 36-37 1.2090339905967156 1101 38-39 1.3099800687144523 1101 40-41 1.364219485066279 1101 42-43 1.2588304985779288 1101 44-45 1.1998319891055047 1101 46-47 1.2606433921093583 1101 48-49 1.352214515064361 1101 50-51 1.2547754436418117 1101 52-53 1.2098593692252848 1101 54-55 1.3272756537166472 1101 56-57 1.2960080501742937 1101 58-59 1.2384747872812731 1101 60-61 1.1867409705836032 1101 62-63 1.153546051999907 1101 64-65 1.25240191884021 1101 66-67 1.1456256918778962 1101 68-69 1.2678275236699719 1101 70-71 1.2910694464471426 1101 72-73 1.2199458914453984 1101 74-75 1.1782063812240153 1101 76-77 1.1262040913673559 1101 78-79 1.149242890070802 1101 80-81 1.1397849887937035 1101 82-83 1.1339364555642497 1101 84-85 1.199698167996246 1101 86-87 1.1829247051111835 1101 88-89 1.185028282038239 1101 90-91 1.21475637259854 1101 92-93 1.109583236149522 1101 94-95 1.0311763163858672 1101 96-97 1.0627819371824856 1101 98-99 1.046368548613195 1101 100-101 1.105577707871415 1101 102-103 1.1512882482225208 1101 104-105 1.0643234763418832 1101 106-107 1.0168055485535028 1101 108-109 0.9998191460298571 1101 110-111 1.0658842607476906 1101 112-113 1.1199042823966856 1101 114-115 1.078420106831203 1101 116-117 1.108126309112265 1101 118-119 1.1583660995795917 1101 120-121 1.1611553384343019 1101 122-123 1.087395462678181 1101 124-125 0.8914686064161472 1102 1 0.3923300720171099 1102 2 -0.6198871742630452 1102 3 -0.020720671989892026 1102 4 0.27148169372108555 1102 5 0.6809455533414166 1102 6 0.5837196205313901 1102 7 0.4901047371010918 1102 8 0.5737397027118334 1102 9 0.8832121824069716 1102 10-11 1.0428955145170349 1102 12-13 1.020268844294904 1102 14-15 1.0742924496415185 1102 16-17 1.2024903963875744 1102 18-19 1.0119496173967164 1102 20-21 0.9458673949505112 1102 22-23 0.9779865704019883 1102 24-25 0.9873176114998898 1102 26-27 1.0296645949147276 1102 28-29 1.0993586779773423 1102 30-31 1.052596229936448 1102 32-33 1.033589961842317 1102 34-35 0.9295416053552614 1102 36-37 0.8332856882772859 1102 38-39 0.6377953522491566 1102 40-41 0.7645621078725213 1102 42-43 0.5542716750485184 1102 44-45 0.5208755114619734 1102 46-47 0.47904183994427285 1102 48-49 0.5619636111045807 1102 50-51 0.5033399114079273 1102 52-53 0.4874336703100397 1102 54-55 0.5477133025392931 1102 56-57 0.33051616376455684 1102 58-59 0.49101160710665326 1102 60-61 0.4203914247682796 1102 62-63 0.5770168166175971 1102 64-65 0.5134602132224302 1102 66-67 0.4530460993212344 1102 68-69 0.3254578261657812 1102 70-71 0.3095582902605294 1102 72-73 0.265124037666407 1102 74-75 0.35257206602867086 1102 76-77 0.42351161407659177 1102 78-79 0.430738612797672 1102 80-81 0.3360891161417925 1102 82-83 0.3200699329432055 1102 84-85 0.4468019690369047 1102 86-87 0.4118172441357899 1102 88-89 0.4746190748771113 1102 90-91 0.4719496877056919 1102 92-93 0.4853229398270926 1102 94-95 0.3348148647587408 1102 96-97 0.22153800032738502 1102 98-99 0.26024344939810007 1102 100-101 0.0827789601864346 1102 102-103 0.14622519151558322 1102 104-105 0.21864638841525874 1102 106-107 0.4134247389582981 1102 108-109 0.5748663283210647 1102 110-111 0.45494039444555057 1102 112-113 0.4575323801125357 1102 114-115 0.5233131750030005 1102 116-117 0.5845916058756444 1102 118-119 0.44702735718608366 1102 120-121 0.4977961356827372 1102 122-123 0.5747256946210335 1102 124-125 0.45306420567533934 1103 1 0.2791815180889472 1103 2 -1.0455743306848326 1103 3 -0.03074386552133035 1103 4 0.25883673414958963 1103 5 0.428002793951876 1103 6 0.5441906670594108 1103 7 0.6765756042316298 1103 8 0.7045204661762554 1103 9 0.6653236907553648 1103 10-11 0.8601855874292994 1103 12-13 0.9355676051708741 1103 14-15 0.9175695290505317 1103 16-17 1.0730909935249002 1103 18-19 1.1357607887341246 1103 20-21 1.1075327161360775 1103 22-23 1.0321479512500176 1103 24-25 1.06136904732044 1103 26-27 1.093476278816368 1103 28-29 1.0813305125728618 1103 30-31 1.1018267428851019 1103 32-33 0.999776099542693 1103 34-35 1.087403699948993 1103 36-37 1.0846292472670243 1103 38-39 0.9668385229714858 1103 40-41 1.1064693593749766 1103 42-43 1.2026948993338138 1103 44-45 1.0783454944192101 1103 46-47 1.0154236490397182 1103 48-49 1.0129599078280123 1103 50-51 1.134633239103053 1103 52-53 0.921271879410611 1103 54-55 0.9319532338556868 1103 56-57 0.9462839209116254 1103 58-59 0.8385698792526348 1103 60-61 0.7767183894910481 1103 62-63 0.8827370334322673 1103 64-65 0.8864852391709981 1103 66-67 0.7463045982768861 1103 68-69 0.6636169517312034 1103 70-71 0.7302479454329429 1103 72-73 0.7151041758024661 1103 74-75 0.6628515416754617 1103 76-77 0.6493179416820212 1103 78-79 0.6786723382426452 1103 80-81 0.7869713673238934 1103 82-83 0.8208157138988845 1103 84-85 0.818159879825096 1103 86-87 0.8151924795949839 1103 88-89 0.6613936363143438 1103 90-91 0.7643842396604867 1103 92-93 0.8582428901083645 1103 94-95 0.7609364193660504 1103 96-97 0.7942093569567596 1103 98-99 0.7442642595058651 1103 100-101 0.7593547748432066 1103 102-103 0.7321348008134976 1103 104-105 0.7730435488094187 1103 106-107 0.7923891029301586 1103 108-109 0.80222444413495 1103 110-111 0.7623431033475079 1103 112-113 0.784548359302427 1103 114-115 0.7663954800043449 1103 116-117 0.7682164601151911 1103 118-119 0.7958042507905638 1103 120-121 0.707254227149825 1103 122-123 0.7938917526176752 1103 124-125 0.643784230278424 1104 1 0.15189975526059385 1104 2 -0.8778173403856577 1104 3 -0.06296524119465019 1104 4 0.13056341172733 1104 5 0.46038172178201364 1104 6 0.3252213873784626 1104 7 0.44059225929743917 1104 8 0.5485156126292168 1104 9 0.5350703845115916 1104 10-11 0.6609393294889827 1104 12-13 0.5433481734246612 1104 14-15 0.6062594005632178 1104 16-17 0.8967333015307446 1104 18-19 0.7347983880468547 1104 20-21 0.6925454300464438 1104 22-23 0.6205805659573969 1104 24-25 0.6166334057304397 1104 26-27 0.5228482335918443 1104 28-29 0.7681611118667817 1104 30-31 0.7326546050042353 1104 32-33 0.8506815933315366 1104 34-35 0.8183320505581975 1104 36-37 0.7887078885246837 1104 38-39 0.7723674279134514 1104 40-41 0.9001136583384195 1104 42-43 0.863166474102897 1104 44-45 0.7783207250254023 1104 46-47 0.7902157742537099 1104 48-49 0.8264016934818272 1104 50-51 0.8738534578262076 1104 52-53 0.6040531531218178 1104 54-55 0.8071699660515108 1104 56-57 0.7772085090812553 1104 58-59 0.6945480038019234 1104 60-61 0.7088067472488824 1104 62-63 0.6225945153568802 1104 64-65 0.7154682517563558 1104 66-67 0.6577481366004676 1104 68-69 0.5569239636644063 1104 70-71 0.5469003461829232 1104 72-73 0.6105087477936131 1104 74-75 0.5996609772468204 1104 76-77 0.5548517064417595 1104 78-79 0.550782314351153 1104 80-81 0.49375974891051655 1104 82-83 0.5816864207985688 1104 84-85 0.6504981262774336 1104 86-87 0.6208279869913085 1104 88-89 0.5658296309465953 1104 90-91 0.5544514775908915 1104 92-93 0.5469876305313903 1104 94-95 0.5498235838034731 1104 96-97 0.5266763885800678 1104 98-99 0.5887892828643473 1104 100-101 0.5602488245450346 1104 102-103 0.5490211782739749 1104 104-105 0.5305931189506978 1104 106-107 0.5928516478442809 1104 108-109 0.5192795826867886 1104 110-111 0.3884044248416991 1104 112-113 0.39414430464671923 1104 114-115 0.5243761630125405 1104 116-117 0.5619414947609904 1104 118-119 0.48560952886620257 1104 120-121 0.44448936708122133 1104 122-123 0.496158152151132 1104 124-125 0.5265669932886254 1105 1 0.6300124650341488 1105 2 0.31334394266987786 1105 3 0.6141208911550571 1105 4 0.5622555375683902 1105 5 0.8137695833470744 1105 6 0.7570135992595937 1105 7 0.8018037957233304 1105 8 0.8376611289103337 1105 9 0.9084900429026703 1105 10-11 0.9253147383150164 1105 12-13 1.0491382356612178 1105 14-15 0.9984339954013564 1105 16-17 1.1198378076417583 1105 18-19 1.0593787730829405 1105 20-21 1.1630673770490176 1105 22-23 1.087215781858255 1105 24-25 1.1999285706586562 1105 26-27 1.1017994947438012 1105 28-29 1.2527853515521485 1105 30-31 1.2034450879414322 1105 32-33 1.1376526979828512 1105 34-35 1.223438270685051 1105 36-37 1.218725015112149 1105 38-39 1.1605401078851116 1105 40-41 0.9197746279041255 1105 42-43 0.9712636383091393 1105 44-45 1.1009353523796293 1105 46-47 1.0387338223112934 1105 48-49 1.029335809046934 1105 50-51 1.0335383598300965 1105 52-53 0.9218651772890354 1105 54-55 0.9133685489688972 1105 56-57 0.8657174781475518 1105 58-59 0.6932762325436492 1105 60-61 0.9978716857858778 1105 62-63 0.9859972575108138 1105 64-65 1.0490906471443928 1105 66-67 0.8954126169808916 1105 68-69 0.8670059527913345 1105 70-71 0.8540454037646263 1105 72-73 0.9236299597914552 1105 74-75 0.9705966358333171 1105 76-77 1.0003132964730526 1105 78-79 0.9054858832497032 1105 80-81 0.6955138670269712 1105 82-83 0.7745863171162171 1105 84-85 0.7010509065658397 1105 86-87 0.7453774301736544 1105 88-89 0.8815863289079644 1105 90-91 0.8236403463947184 1105 92-93 0.6322794271402685 1105 94-95 0.8074643895944824 1105 96-97 0.9124378431728957 1105 98-99 0.9380098331407858 1105 100-101 0.8500705153273493 1105 102-103 0.4383869177707105 1105 104-105 0.428776268939977 1105 106-107 0.6961511283468411 1105 108-109 0.7549184233986708 1105 110-111 0.6676298878159734 1105 112-113 0.5416470392008996 1105 114-115 0.644692984524184 1105 116-117 0.3619349806865202 1105 118-119 0.2574097951289964 1105 120-121 0.3033877285200717 1105 122-123 0.36216499735772345 1105 124-125 0.17482597117605891 1106 1 0.5279411271026397 1106 2 0.420275306671158 1106 3 0.569584320106312 1106 4 0.5156968331149514 1106 5 0.5796617822714794 1106 6 0.6390586947768 1106 7 0.6268968095437586 1106 8 0.6761105820494961 1106 9 0.6896960717520599 1106 10-11 0.8951996256193766 1106 12-13 0.771120789635134 1106 14-15 0.9091277792294647 1106 16-17 1.0132021024200384 1106 18-19 0.8969326175103092 1106 20-21 0.9858857605508291 1106 22-23 0.9926859008986852 1106 24-25 0.9662772063120855 1106 26-27 0.9349821212296519 1106 28-29 1.0845835611979666 1106 30-31 1.0286503226124992 1106 32-33 0.9862592171510371 1106 34-35 0.8774236215478624 1106 36-37 0.9531101311743981 1106 38-39 1.005890064291627 1106 40-41 0.9809954181115614 1106 42-43 0.8820912239207033 1106 44-45 0.8534225548864711 1106 46-47 0.9250767605984578 1106 48-49 0.9088645677980125 1106 50-51 0.963680880310406 1106 52-53 1.1133042370509543 1106 54-55 1.107498751052745 1106 56-57 0.9341808294074454 1106 58-59 0.8898375267090266 1106 60-61 1.0311796974913783 1106 62-63 0.9990839351693701 1106 64-65 0.9018568911125193 1106 66-67 0.8470011894453435 1106 68-69 0.9185552479580643 1106 70-71 1.074896037812433 1106 72-73 1.1192816176387126 1106 74-75 1.0305463090979963 1106 76-77 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-2.845493194933894 2216 74-75 -2.51796114377348 2216 76-77 -2.763188611705914 2216 78-79 -2.4660127698964267 2216 80-81 -2.0749920402669275 2216 82-83 -2.078789775917542 2216 84-85 -1.9989735371838364 2216 86-87 -2.158445307930986 2216 88-89 -2.0091080922385878 2216 90-91 -2.043901454375341 2216 92-93 -2.0724950670307614 2216 94-95 -1.8504516975257808 2216 96-97 -1.9296253463676614 2216 98-99 -1.8374451991713059 2216 100-101 -2.061414065529245 2216 102-103 -1.764262699842888 2216 104-105 -1.8461203754455653 2216 106-107 -1.9680068448518426 2216 108-109 -2.0324929965645318 2216 110-111 -1.9200660619624053 2216 112-113 -1.987815511603916 2216 114-115 -2.0113611024120175 2216 116-117 -2.0742189405282403 2216 118-119 -2.2635929011895612 2216 120-121 -2.085872050122031 2216 122-123 -2.0846278292992118 2216 124-125 -2.1604324570223064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 83638.0 3 26164.0 4 12178.0 5 9499.0 6 10163.0 7 12053.0 8 13799.0 9 14647.0 10 15905.0 11 17337.0 12 19444.0 13 21272.0 14 23259.0 15 19587.0 16 13220.0 17 11376.0 18 10410.0 19 10002.0 20 9694.0 21 10257.0 22 12126.0 23 15811.0 24 22291.0 25 29312.0 26 34636.0 27 39302.0 28 45556.0 29 53165.0 30 61810.0 31 73364.0 32 88397.0 33 110194.0 34 141477.0 35 193044.0 36 305006.0 37 464866.0 38 184093.0 39 845.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.493559738591294 20.26556443054237 13.749695581746076 24.49118024912026 2 15.319056510511869 21.307349146349203 38.796129365569556 24.577464977569377 3 19.454903293543808 25.367106719858306 28.911320521311417 26.26666946528647 4 13.081097255966842 17.515051093325713 36.42458375017865 32.9792679005288 5 13.358771635548525 38.65208863142857 33.67955869221158 14.309581040811322 6 31.485955648287415 37.65599376918103 16.98813679220004 13.869913790331513 7 28.160739621623627 32.749523378672464 20.702626251619442 18.38711074808447 8 25.852816118620993 36.8583140667712 18.974865565767043 18.314004248840767 9 26.05385441578074 16.618725116504443 19.789030383822688 37.53839008389213 10-11 24.843250610229514 27.596740229345755 26.798576782791805 20.761432377632925 12-13 25.664293874838435 24.54021761558986 27.839400194908965 21.956088314662747 14-15 22.616566229680547 25.84195343648129 25.850171211081136 25.691309122757026 16-17 22.010494017748588 29.057540156842805 26.455214994211445 22.47675083119716 18-19 21.83125103831911 27.519676705388896 29.09414807814948 21.554924178142514 20-21 22.66099475387519 26.53310471793928 29.24542627778825 21.560474250397277 22-23 22.302908085558727 26.479568365245896 29.06772431473963 22.149799234455745 24-25 22.108173503114283 26.63635523238444 29.2522906628665 22.00318060163478 26-27 21.920159842872383 26.840803340837503 29.128518724776136 22.110518091513974 28-29 21.824158958210692 26.779561430650705 29.316655291551104 22.079624319587502 30-31 21.944186291615885 26.58803438193747 29.35225944634666 22.11551988009998 32-33 21.87618629087651 26.635842084531347 29.253631557369363 22.234340067222778 34-35 22.180230519931456 26.631777702651704 29.17016308063732 22.017828696779517 36-37 22.262963522852953 26.454801979192087 29.070383059280413 22.211851438674543 38-39 22.103104726288283 26.737127874744495 29.03453422406852 22.125233174898703 40-41 22.226854334965314 26.609492948147977 29.072114626703566 22.091538090183143 42-43 22.027592884372847 26.53450361033402 28.94488390123298 22.49301960406015 44-45 22.257490290054612 26.781675054338628 28.690326317580528 22.270508338026232 46-47 22.34418305125317 27.030816390511248 28.1750430287987 22.44995752943688 48-49 22.342220677024404 27.0567710590701 28.035984352415856 22.56502391148964 50-51 22.479569630281652 27.23164303536411 27.890876660365283 22.397910673988957 52-53 22.61772140931866 27.13119511002947 27.761041856327417 22.49004162432445 54-55 22.38936780518391 27.198073954123775 27.836538869479664 22.576019371212652 56-57 22.412060234181517 27.445241196421605 27.68475060184386 22.457947967553018 58-59 22.47695380220954 27.041221901770186 27.84400932833988 22.63781496768039 60-61 22.52964117972543 27.084015310832132 28.003026975271066 22.38331653417137 62-63 22.446352468003067 27.050744484969847 28.126620278054787 22.376282768972295 64-65 22.312783263765752 26.960514061002876 28.052940584081874 22.6737620911495 66-67 22.228462052725103 27.251798522596697 27.893434214645506 22.626305210032694 68-69 22.349478259180287 26.979262334780955 28.112441728279446 22.55881767775931 70-71 22.251372030802084 26.960533655115064 27.94950337151812 22.83859094256473 72-73 22.412459859362347 27.08776533684418 27.772643160365824 22.727131643427654 74-75 22.27499083905748 27.2048395679474 27.761083512290845 22.759086080704275 76-77 22.435672566199763 27.04227446684368 27.868265668566984 22.65378729838957 78-79 22.447518141139707 27.0664219042832 27.808323454338275 22.677736500238815 80-81 22.205370740051002 27.27316371691296 27.767409352572603 22.75405619046343 82-83 22.439487753525764 27.31318011312545 27.61473931150719 22.632592821841595 84-85 22.348542279097757 27.18440423410619 27.69983442728924 22.76721905950681 86-87 22.371522208583862 27.123395709297622 27.86958054707717 22.635501535041346 88-89 22.418463030753408 27.068920627420788 27.779732841967146 22.732883499858655 90-91 22.327827942045346 27.31313742101528 27.80701938505689 22.55201525188248 92-93 22.336206422983288 27.233165092563688 27.730451222012164 22.700177262440864 94-95 22.273753769123633 27.076727667126004 27.791395712929614 22.85812285082075 96-97 22.396833329537117 27.051464647615013 27.702694555018574 22.849007467829292 98-99 22.168388127103444 26.995369741834523 27.629618646792757 23.20662348426927 100-101 22.088592402378097 27.039897284171154 27.852870739944734 23.018639573506015 102-103 22.138620768039235 26.94137247060384 27.84075798651793 23.079248774838998 104-105 22.063772139134176 26.88759020556978 27.942949038291058 23.10568861700498 106-107 22.066195168964757 26.999525703562043 27.761950528469843 23.172328599003354 108-109 22.028853142839917 26.96278299627417 27.591965066282288 23.41639879460363 110-111 22.002343261715808 26.958495864457916 27.764480537909638 23.274680335916635 112-113 22.05938736031787 26.975099664090756 27.730454438778345 23.23505853681302 114-115 21.92560977352545 26.9509269415039 27.708767269622257 23.414696015348394 116-117 21.726218920181463 26.99200233659677 27.813763591070558 23.468015152151214 118-119 21.649797613792142 27.007432922933805 27.70155498078632 23.641214482487737 120-121 21.610185294156402 27.05513982313612 27.79262722517462 23.542047657532862 122-123 21.655246948384065 27.00858460621231 27.640645637414547 23.695522807989075 124-125 21.535677872203028 26.900432796033876 27.70450628605982 23.85938304570328 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 287.0 1 207.0 2 263.5 3 511.0 4 432.0 5 307.5 6 752.0 7 1758.0 8 2711.0 9 3022.0 10 2470.0 11 1699.5 12 1524.0 13 1678.0 14 1876.0 15 2041.0 16 2239.5 17 2471.5 18 2692.5 19 2985.0 20 3396.0 21 4021.5 22 4877.0 23 5901.5 24 7307.5 25 9232.0 26 12035.0 27 15445.0 28 18540.5 29 22223.0 30 26764.5 31 31303.0 32 35939.0 33 40540.5 34 45361.0 35 50140.5 36 55447.0 37 60712.5 38 63968.0 39 66320.5 40 68430.5 41 70666.0 42 73182.5 43 75657.0 44 78292.0 45 80727.5 46 81354.0 47 82005.5 48 84858.0 49 85440.0 50 83134.5 51 79600.0 52 76307.5 53 74913.5 54 73407.5 55 69084.5 56 62877.0 57 57300.5 58 52612.5 59 47464.5 60 40168.5 61 32265.5 62 25220.0 63 18714.5 64 13701.0 65 10256.5 66 7489.5 67 5630.0 68 4356.0 69 3321.5 70 2520.0 71 1887.5 72 1428.0 73 1129.0 74 839.0 75 605.0 76 428.5 77 247.0 78 138.5 79 83.0 80 57.5 81 37.5 82 25.0 83 19.0 84 17.0 85 14.5 86 10.0 87 4.5 88 1.5 89 1.5 90 2.0 91 2.5 92 1.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0591282864988775 2 0.07292786393705963 3 0.0 4 0.007100753439064594 5 0.025902119463254495 6 3.1261178662548526E-4 7 0.0 8 0.0 9 6.252235732509705E-4 10-11 0.058570051165617705 12-13 0.00915505946546064 14-15 0.006698823999117542 16-17 0.05189355657983055 18-19 1.3397647998235083E-4 20-21 0.00951233007874691 22-23 0.012013224371750791 24-25 0.0 26-27 0.0 28-29 0.05030816823337273 30-31 0.0 32-33 0.0034387296528803376 34-35 0.0 36-37 1.1164706665195903E-4 38-39 0.0 40-41 0.0 42-43 0.0031707766929156365 44-45 0.0 46-47 4.4658826660783614E-5 48-49 1.5630589331274263E-4 50-51 3.572706132862689E-4 52-53 1.5630589331274263E-4 54-55 0.0 56-57 0.0017193648264401688 58-59 4.4658826660783614E-5 60-61 0.0 62-63 0.0 64-65 4.242588532774443E-4 66-67 0.0 68-69 5.582353332597952E-4 70-71 0.0 72-73 0.0018086824797617365 74-75 0.003282423759567595 76-77 4.689176799382279E-4 78-79 9.15505946546064E-4 80-81 0.0012951059731627248 82-83 1.5630589331274263E-4 84-85 6.698823999117541E-5 86-87 3.7960002661666066E-4 88-89 0.0 90-91 0.0 92-93 0.006520188692474407 94-95 0.005247412132642074 96-97 0.0057163298125803026 98-99 6.698823999117541E-4 100-101 5.135765065990115E-4 102-103 1.5630589331274263E-4 104-105 3.572706132862689E-4 106-107 0.004153270879452876 108-109 0.004532870906069536 110-111 5.582353332597952E-4 112-113 0.0023445883996911394 114-115 3.1261178662548526E-4 116-117 0.0020096471997352624 118-119 0.0017640236531009524 120-121 0.010963741945222377 122-123 7.368706399029296E-4 124-125 0.0016970354131097772 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2239199.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.970166103706966 #Duplication Level Percentage of deduplicated Percentage of total 1 79.9999693087215 48.7761141704421 2 11.595164976251015 14.139182692038196 3 3.5889491641404536 6.564564800262112 4 1.6056211265266647 3.9157994713580737 5 0.845019052307833 2.5760475990002814 6 0.5013351829567063 1.8339893627101624 7 0.3425936809545352 1.462159554371488 8 0.23440404878057358 1.1433323031626401 9 0.18404182006391412 1.009895430539285 >10 1.0019883944396986 11.30868459553166 >50 0.07009843395493554 2.928374178847452 >100 0.029486164724747913 3.12223873774746 >500 9.595777947370782E-4 0.33933258170036695 >1k 2.214410295546133E-4 0.32335604768118975 >5k 1.4762735303640886E-4 0.5569284746074771 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6613 0.295328820707762 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5762 0.2573241592194352 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3953 0.1765363417900776 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 4.4658826660783614E-5 0.0 0.0 0.0 12-13 4.4658826660783614E-5 4.4658826660783614E-5 0.0 0.0 0.0 14-15 8.931765332156723E-5 4.4658826660783614E-5 0.0 0.0 0.0 16-17 8.931765332156723E-5 4.4658826660783614E-5 0.0 0.0 0.0 18-19 8.931765332156723E-5 4.4658826660783614E-5 0.0 0.0 0.0 20-21 8.931765332156723E-5 4.4658826660783614E-5 0.0 4.4658826660783614E-5 0.0 22-23 8.931765332156723E-5 4.4658826660783614E-5 0.0 4.4658826660783614E-5 0.0 24-25 2.6795295996470166E-4 4.4658826660783614E-5 0.0 8.931765332156723E-5 0.0 26-27 2.6795295996470166E-4 4.4658826660783614E-5 0.0 2.6795295996470166E-4 0.0 28-29 3.349411999558771E-4 4.4658826660783614E-5 0.0 4.242588532774443E-4 0.0 30-31 4.019294399470525E-4 4.4658826660783614E-5 0.0 0.0013397647998235084 0.0 32-33 4.242588532774443E-4 4.4658826660783614E-5 0.0 0.0031484472795852442 0.0 34-35 4.465882666078361E-4 4.4658826660783614E-5 0.0 0.0057163298125803026 0.0 36-37 4.465882666078361E-4 4.4658826660783614E-5 0.0 0.01114237725186551 0.0 38-39 4.912470932686197E-4 4.4658826660783614E-5 0.0 0.021034307357229082 0.0 40-41 4.912470932686197E-4 4.4658826660783614E-5 0.0 0.03228833167574655 0.0 42-43 5.359059199294033E-4 4.4658826660783614E-5 0.0 0.043921956020880684 0.0 44-45 5.805647465901869E-4 4.4658826660783614E-5 0.0 0.05555558036601481 0.0 46-47 7.145412265725378E-4 4.4658826660783614E-5 0.0 0.07094054615065476 0.0 48-49 7.145412265725378E-4 4.4658826660783614E-5 0.0 0.0896079356948623 0.0 50-51 7.145412265725378E-4 4.4658826660783614E-5 0.0 0.11321012558508645 0.0 52-53 7.145412265725378E-4 4.4658826660783614E-5 0.0 0.13475800944891456 0.0 54-55 7.145412265725378E-4 4.4658826660783614E-5 0.0 0.1569757757126544 8.931765332156723E-5 56-57 8.03858879894105E-4 4.4658826660783614E-5 0.0 0.1825876128026138 8.931765332156723E-5 58-59 8.03858879894105E-4 4.4658826660783614E-5 0.0 0.21295561493194665 8.931765332156723E-5 60-61 8.03858879894105E-4 4.4658826660783614E-5 0.0 0.24953119396712842 8.931765332156723E-5 62-63 8.03858879894105E-4 4.4658826660783614E-5 0.0 0.2886299967086445 8.931765332156723E-5 64-65 8.03858879894105E-4 4.4658826660783614E-5 0.0 0.3283540230234115 8.931765332156723E-5 66-67 8.485177065548886E-4 4.4658826660783614E-5 0.0 0.3719410378443363 8.931765332156723E-5 68-69 8.485177065548886E-4 4.4658826660783614E-5 0.0 0.4281441711969325 8.931765332156723E-5 70-71 9.15505946546064E-4 4.4658826660783614E-5 0.0 0.4901529520154305 8.931765332156723E-5 72-73 0.0010048235998676312 4.4658826660783614E-5 0.0 0.5577887449931873 8.931765332156723E-5 74-75 0.001027153013198023 4.4658826660783614E-5 0.0 0.6261167497841862 8.931765332156723E-5 76-77 0.001027153013198023 4.4658826660783614E-5 0.0 0.6980174607080478 8.931765332156723E-5 78-79 0.001027153013198023 4.4658826660783614E-5 0.0 0.7848788785632719 8.931765332156723E-5 80-81 0.0011164706665195902 4.4658826660783614E-5 0.0 0.8890902505762105 8.931765332156723E-5 82-83 0.0011611294931803738 4.4658826660783614E-5 0.0 0.9993082347750244 8.931765332156723E-5 84-85 0.0011834589065107656 4.4658826660783614E-5 0.0 1.110531042573706 1.1164706665195903E-4 86-87 0.0012057883198411574 4.4658826660783614E-5 0.0 1.2307302745312052 1.3397647998235083E-4 88-89 0.0012057883198411574 4.4658826660783614E-5 0.0 1.3703114372594842 1.3397647998235083E-4 90-91 0.0012951059731627246 4.4658826660783614E-5 0.0 1.530725942625019 1.3397647998235083E-4 92-93 0.0012951059731627246 4.4658826660783614E-5 0.0 1.7115941906011924 1.3397647998235083E-4 94-95 0.0012951059731627246 4.4658826660783614E-5 0.0 1.891770226764124 1.3397647998235083E-4 96-97 0.0012951059731627246 4.4658826660783614E-5 0.0 2.073174380660227 1.5630589331274265E-4 98-99 0.0013397647998235082 4.4658826660783614E-5 0.0 2.281128207006166 1.7863530664313445E-4 100-101 0.0013620942131539 4.4658826660783614E-5 0.0 2.5153860822553065 1.7863530664313445E-4 102-103 0.0013844236264842918 4.4658826660783614E-5 0.0 2.779565371367172 1.7863530664313445E-4 104-105 0.00160771775978821 4.4658826660783614E-5 0.0 3.0454863547188076 1.7863530664313445E-4 106-107 0.0016523765864489936 4.4658826660783614E-5 0.0 3.30834820844418 2.0096471997352625E-4 108-109 0.0016523765864489936 4.4658826660783614E-5 0.0 3.5978713816860406 2.4562354663430985E-4 110-111 0.0016523765864489936 4.4658826660783614E-5 0.0 3.9370328407613613 2.6795295996470166E-4 112-113 0.0016523765864489936 4.4658826660783614E-5 0.0 4.30687491375264 2.6795295996470166E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5450 0.0 52.244255 1 GTATCAA 8775 0.0 43.17349 1 GTACCGT 190 2.910383E-11 34.469315 6 ATCAACG 11035 0.0 34.183723 3 TCAACGC 11215 0.0 33.68813 4 TATCAAC 11320 0.0 33.13431 2 CAACGCA 11285 0.0 32.982136 5 AACGCAG 11710 0.0 32.031494 6 GTACATG 9925 0.0 31.269026 1 TACATGG 9885 0.0 30.054377 2 TTTACGC 120 3.698408E-5 29.749004 3 ACATGGG 10380 0.0 27.80013 3 ACGCAGA 13615 0.0 27.244783 7 CGCAGAG 13785 0.0 26.8656 8 GAGTACT 8565 0.0 24.43656 12-13 GTACTTT 8865 0.0 23.475266 14-15 AGTACTT 9025 0.0 23.388971 12-13 GCAGAGT 15215 0.0 23.362288 9 TACCTGG 2170 0.0 23.046415 2 CATGGGA 6315 0.0 22.98894 4 >>END_MODULE