FastQCFastQC Report
Thu 26 May 2016
SRR1364633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3453559
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA71600.042.5898361
TCAACGC83150.036.815524
ATCAACG82850.036.7337073
CAACGCA84350.036.153185
AACGCAG86000.034.047226
GTACATG124200.033.7118641
CGCATAG3050.033.163728
TATCAAC93950.032.3303032
TACATGG123400.032.0954062
ACATGGG130500.030.0753923
ACGCAGA101450.028.3886857
GGTATCA47150.028.169051
CGCAGAG100800.026.5623288
ACGCATA4450.024.0693557
CATGGGG67600.022.9063954
CATGGGA75750.021.699844
GCAGAGT118550.021.4801589
CCAATAC22800.021.41983
ATACAGT38550.021.3044176
AACGCAT6000.019.8378096
Produced by FastQC (version 0.11.5)