FastQCFastQC Report
Thu 26 May 2016
SRR1364633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3453559
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70730.20480321894022951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55790.16154349759190445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37120.10748332372488786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66900.047.8253361
GTATCAA104500.044.757231
ATCAACG130000.035.562283
TCAACGC131750.034.77484
TATCAAC135900.034.2665562
CAACGCA133600.033.795045
AACGCAG138500.032.719416
GTACATG126050.029.1170751
TACATGG124700.028.2708232
ACGCAGA166150.027.0242827
CGCAGAG166950.026.7164388
ACATGGG132900.025.7427443
GCAGAGT186850.022.9474899
GAGTACT105750.021.96591412-13
GTACTTT110500.020.99472614-15
CATGGGG66400.020.7895184
AGTACTT111700.020.52922612-13
AGAGTAC181700.020.46352410-11
ATTCGCG3200.019.51925580-81
CAGAGTA189700.019.11366810-11
Produced by FastQC (version 0.11.5)