##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364633_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3453559 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.736199960678245 31.0 30.0 33.0 26.0 34.0 2 29.783888736228338 31.0 30.0 34.0 26.0 34.0 3 29.807837943408526 31.0 30.0 34.0 26.0 34.0 4 33.5111744145677 35.0 33.0 37.0 28.0 37.0 5 32.9700407608499 35.0 33.0 37.0 26.0 37.0 6 33.01893177443906 35.0 33.0 37.0 26.0 37.0 7 32.832648291226526 35.0 33.0 37.0 26.0 37.0 8 32.776906084419004 35.0 33.0 37.0 25.0 37.0 9 34.07449040250941 38.0 34.0 39.0 25.0 39.0 10-11 33.88047996284413 37.0 33.0 39.0 24.5 39.0 12-13 33.74572187705495 37.0 33.0 39.0 23.5 39.0 14-15 34.76309424567526 38.0 33.0 41.0 24.0 41.0 16-17 34.68944297751971 38.5 33.0 41.0 23.0 41.0 18-19 34.70100568717662 38.5 33.5 41.0 23.0 41.0 20-21 34.71445051322418 39.0 34.0 41.0 23.0 41.0 22-23 34.70577468055417 39.0 34.0 41.0 23.0 41.0 24-25 34.70630934638731 39.0 34.0 41.0 23.5 41.0 26-27 34.62629305015493 39.0 34.0 41.0 23.0 41.0 28-29 34.393559079199164 39.0 33.0 41.0 21.5 41.0 30-31 34.34816793342752 38.5 33.0 41.0 22.0 41.0 32-33 34.20114134433493 38.0 33.0 41.0 21.5 41.0 34-35 34.08722900057593 38.0 33.0 41.0 19.5 41.0 36-37 33.93589337839603 38.0 33.0 40.0 18.5 41.0 38-39 33.81428433682471 38.0 33.0 40.0 17.5 41.0 40-41 33.657312499945704 38.0 33.0 40.0 16.0 41.0 42-43 33.49849980845846 38.0 33.0 40.0 15.0 41.0 44-45 33.32410261414384 38.0 32.0 40.0 14.0 41.0 46-47 33.1102478631464 38.0 32.0 40.0 9.0 41.0 48-49 32.89756538110396 38.0 32.0 40.0 8.0 41.0 50-51 32.71094065571198 38.0 31.0 40.0 7.0 41.0 52-53 32.54627371358069 38.0 31.0 40.0 4.0 41.0 54-55 32.32936110256115 37.5 31.0 40.0 2.0 41.0 56-57 32.115039152364275 37.0 31.0 40.0 2.0 41.0 58-59 31.932026787438698 37.0 31.0 40.0 2.0 41.0 60-61 31.712653092071108 36.5 30.0 40.0 2.0 41.0 62-63 31.44715089564128 36.0 30.0 40.0 2.0 41.0 64-65 31.167082421351424 36.0 30.0 39.0 2.0 41.0 66-67 30.89688521319601 35.0 29.0 39.0 2.0 41.0 68-69 30.58239007933555 35.0 29.0 39.0 2.0 40.0 70-71 30.31938718869433 35.0 29.0 38.0 2.0 40.0 72-73 30.161726786772718 35.0 29.0 37.5 2.0 40.0 74-75 29.870089232585862 35.0 29.0 37.0 2.0 39.0 76-77 29.56971894790273 34.5 29.0 36.5 2.0 39.0 78-79 29.261752875801456 34.0 29.0 36.0 2.0 39.0 80-81 28.975447502127516 34.0 29.0 36.0 2.0 37.5 82-83 28.71898467638746 34.0 29.0 35.0 2.0 37.0 84-85 28.70061782642196 34.0 29.0 35.0 2.0 37.0 86-87 28.55214417938133 34.0 29.0 35.0 2.0 36.0 88-89 28.41089597716443 34.0 29.0 35.0 2.0 36.0 90-91 28.26241436732368 34.0 29.0 35.0 2.0 36.0 92-93 28.13070371752734 34.0 29.0 35.0 2.0 35.5 94-95 28.046611191527347 34.0 29.0 35.0 2.0 35.0 96-97 27.96695901242747 34.0 28.0 35.0 2.0 35.0 98-99 27.87731351918412 34.0 28.0 35.0 2.0 35.0 100-101 27.785461606418192 34.0 27.5 35.0 2.0 35.0 102-103 26.591307546794482 32.5 25.0 34.0 2.0 35.0 104-105 27.534946558028977 34.0 27.0 35.0 2.0 35.0 106-107 27.709761871738692 34.0 27.5 35.0 2.0 35.0 108-109 27.723351910304704 34.0 28.0 35.0 2.0 35.0 110-111 27.67195203556679 34.0 28.0 35.0 2.0 35.0 112-113 27.629028633939654 34.0 28.0 35.0 2.0 35.0 114-115 27.553533470833997 34.0 27.0 35.0 2.0 35.0 116-117 27.43777491567395 34.0 27.0 35.0 2.0 35.0 118-119 27.336892608465643 34.0 27.0 35.0 2.0 35.0 120-121 27.20188883409839 34.0 27.0 35.0 2.0 35.0 122-123 27.018741970239976 34.0 27.0 35.0 2.0 35.0 124-125 26.20243841208446 33.0 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6766591830099955 1101 2 0.6717312226626824 1101 3 0.4854504445705814 1101 4 0.6338893045751703 1101 5 0.8960446777633635 1101 6 0.8209528494209124 1101 7 0.9154875781685163 1101 8 0.9061749157277319 1101 9 1.0438672444871386 1101 10-11 1.140123040903866 1101 12-13 1.042713634733012 1101 14-15 1.144724051084232 1101 16-17 1.2415165612216796 1101 18-19 1.241100475733191 1101 20-21 1.2620193677586045 1101 22-23 1.1818671671990089 1101 24-25 1.1579251639908108 1101 26-27 1.2103571247730045 1101 28-29 1.3528378649475457 1101 30-31 1.3177766310662378 1101 32-33 1.3925987761017637 1101 34-35 1.3683817399061553 1101 36-37 1.3271323220473334 1101 38-39 1.4443756643190255 1101 40-41 1.427733968484361 1101 42-43 1.3906891354955562 1101 44-45 1.3289832441635312 1101 46-47 1.3370482433452366 1101 48-49 1.4227783166668075 1101 50-51 1.3354705735546446 1101 52-53 1.4624586949420717 1101 54-55 1.3993630257334502 1101 56-57 1.4000660392881414 1101 58-59 1.3504345697259907 1101 60-61 1.3484160293811378 1101 62-63 1.3269409359045703 1101 64-65 1.360619826567845 1101 66-67 1.2656776353188803 1101 68-69 1.2727278513138849 1101 70-71 1.3360352826149935 1101 72-73 1.3085861329591246 1101 74-75 1.2026277983041709 1101 76-77 1.2371763212251956 1101 78-79 1.279105638306131 1101 80-81 1.2336437351942173 1101 82-83 1.1757901453448198 1101 84-85 1.216716986827283 1101 86-87 1.234845582328166 1101 88-89 1.1999837798424942 1101 90-91 1.1818352288691223 1101 92-93 1.2028926126944697 1101 94-95 1.0719055966340392 1101 96-97 1.119694062540919 1101 98-99 1.1888286358106406 1101 100-101 1.2407529588436539 1101 102-103 1.2672507960539434 1101 104-105 1.223988218219045 1101 106-107 1.1808276931475667 1101 108-109 1.1656964422606961 1101 110-111 1.2468559612333223 1101 112-113 1.2319635690103787 1101 114-115 1.1448936466027924 1101 116-117 1.1968036183694686 1101 118-119 1.2703142003802057 1101 120-121 1.3295548601474287 1101 122-123 1.3769697361061937 1101 124-125 1.136023055691446 1102 1 0.20925961939991566 1102 2 -0.7871929451828983 1102 3 -0.2103899203034878 1102 4 0.08718091176743314 1102 5 0.4616534290115908 1102 6 0.32578559356288395 1102 7 0.3242617322569785 1102 8 0.23918347233656334 1102 9 0.5928702275375599 1102 10-11 0.6876096437695409 1102 12-13 0.6642607383879309 1102 14-15 0.792995429045618 1102 16-17 0.940066420681525 1102 18-19 0.8995020318208944 1102 20-21 0.8157936752075514 1102 22-23 0.7807813975869564 1102 24-25 0.8013115192586255 1102 26-27 0.8331026311217542 1102 28-29 0.8648440649420195 1102 30-31 0.7983936781349144 1102 32-33 0.7712331364366634 1102 34-35 0.6289286732780468 1102 36-37 0.6109262883470024 1102 38-39 0.5172723251851892 1102 40-41 0.5149920083136621 1102 42-43 0.4024938137611187 1102 44-45 0.2436709183466732 1102 46-47 0.205804563799461 1102 48-49 0.39849209643178085 1102 50-51 0.3925968276166216 1102 52-53 0.4706054233095216 1102 54-55 0.46928192447518313 1102 56-57 0.34518440110195314 1102 58-59 0.5931281133097528 1102 60-61 0.3010469777095288 1102 62-63 0.40260707570617527 1102 64-65 0.33496986894217784 1102 66-67 0.3323950393775412 1102 68-69 0.1987537007912401 1102 70-71 0.0929263436323744 1102 72-73 0.14974749947210952 1102 74-75 0.20817952362479986 1102 76-77 0.28486844981290105 1102 78-79 0.29092701246749897 1102 80-81 0.190290613069255 1102 82-83 0.1702787243699717 1102 84-85 0.2506558523494711 1102 86-87 0.32633448303261403 1102 88-89 0.3568355639538332 1102 90-91 0.26877899301247155 1102 92-93 0.42601988845461136 1102 94-95 0.22416982452138257 1102 96-97 0.26650500290061174 1102 98-99 0.19238999881824626 1102 100-101 0.016049885151897314 1102 102-103 0.025706436267515897 1102 104-105 0.054702524546879516 1102 106-107 0.3239044944194198 1102 108-109 0.37238490878119634 1102 110-111 0.322926277698361 1102 112-113 0.3651238014112046 1102 114-115 0.4597929733729025 1102 116-117 0.4301788500911279 1102 118-119 0.3882741417133957 1102 120-121 0.485339731187878 1102 122-123 0.4298954992030666 1102 124-125 0.2393681577426534 1103 1 0.22854323133667265 1103 2 -1.0627948695374627 1103 3 -0.1633004687575088 1103 4 0.2371203337230412 1103 5 0.5113395456540033 1103 6 0.539956579126823 1103 7 0.5811909202517924 1103 8 0.686188066757019 1103 9 0.7146811953210275 1103 10-11 0.8526254487179656 1103 12-13 0.7326251883165895 1103 14-15 0.8475247398794608 1103 16-17 0.941790891633012 1103 18-19 1.060699806513803 1103 20-21 1.0565497914130262 1103 22-23 0.9342926622314138 1103 24-25 0.8152809073902674 1103 26-27 0.8468703369022066 1103 28-29 0.9806748518667945 1103 30-31 0.9213623605134345 1103 32-33 0.8643681162390919 1103 34-35 0.9899327093954327 1103 36-37 1.0504206180042814 1103 38-39 1.0552783510292514 1103 40-41 1.0796358917067579 1103 42-43 1.0881964380171283 1103 44-45 1.1365323512617564 1103 46-47 1.1577891876142061 1103 48-49 1.093230591814276 1103 50-51 1.1882023216523052 1103 52-53 1.1587588451777435 1103 54-55 1.1646011878301081 1103 56-57 1.1076706809121433 1103 58-59 1.101646944226836 1103 60-61 1.0838949146978685 1103 62-63 1.0484193240664332 1103 64-65 1.0698835658796462 1103 66-67 0.9406571605061487 1103 68-69 0.9163928122062082 1103 70-71 0.9313901671838991 1103 72-73 1.0064431373742835 1103 74-75 0.9366816903687436 1103 76-77 0.7926870859260653 1103 78-79 0.8393013639381159 1103 80-81 0.9324659168356852 1103 82-83 0.923506992971177 1103 84-85 0.9636311800167086 1103 86-87 0.9745824752027836 1103 88-89 0.9336104574230895 1103 90-91 0.9855754388946707 1103 92-93 0.9213716217030878 1103 94-95 0.8467017922355353 1103 96-97 0.8857387211326468 1103 98-99 0.9368335631942486 1103 100-101 0.9545927759049881 1103 102-103 0.946777266491658 1103 104-105 0.9641158446761011 1103 106-107 0.9540568512504244 1103 108-109 0.9358681054182867 1103 110-111 0.9119787049194024 1103 112-113 0.9445256219794551 1103 114-115 0.9573661460458815 1103 116-117 1.0364682283289461 1103 118-119 1.0440440942931453 1103 120-121 0.9955361732718835 1103 122-123 1.0586794654059695 1103 124-125 1.042842776028408 1104 1 0.31370028820792584 1104 2 -0.7653921239214654 1104 3 -0.05324130635233004 1104 4 0.19851128509861127 1104 5 0.6024867101576348 1104 6 0.46271428274238247 1104 7 0.590217048446867 1104 8 0.6299961054708589 1104 9 0.687085516868386 1104 10-11 0.9232544350400786 1104 12-13 0.812383410570412 1104 14-15 0.8660331947885567 1104 16-17 1.0031210240082586 1104 18-19 0.9834082529382613 1104 20-21 0.9650410589607574 1104 22-23 0.9461900103945382 1104 24-25 0.9610574337507387 1104 26-27 0.8022802926655928 1104 28-29 1.063538293754661 1104 30-31 0.9360860033975271 1104 32-33 0.9260884596756185 1104 34-35 1.0076874433886758 1104 36-37 0.9896451238414983 1104 38-39 0.8759191755087414 1104 40-41 0.9636160490130905 1104 42-43 1.0722967470191733 1104 44-45 1.0073507555707621 1104 46-47 1.0575436709522066 1104 48-49 1.146751461477436 1104 50-51 1.0332144391453753 1104 52-53 0.7804625345301517 1104 54-55 1.010183366750148 1104 56-57 1.087345909093564 1104 58-59 1.0615424413054697 1104 60-61 0.9972023330474435 1104 62-63 1.0002530359718413 1104 64-65 1.0119548311389188 1104 66-67 1.0281114921066035 1104 68-69 0.8887584823564545 1104 70-71 0.9832105994402127 1104 72-73 0.9419605821128663 1104 74-75 0.9694498967078999 1104 76-77 0.9062401479084166 1104 78-79 0.9327459525483306 1104 80-81 0.9012511979056583 1104 82-83 0.9035777351108649 1104 84-85 0.9124951575718647 1104 86-87 0.9809749308085749 1104 88-89 0.9333764577570989 1104 90-91 0.9869651340064749 1104 92-93 0.9925952046868574 1104 94-95 0.9407574463635626 1104 96-97 0.9843839952655387 1104 98-99 0.9976357585637281 1104 100-101 1.0322033995645121 1104 102-103 0.956276248783567 1104 104-105 0.9326869185829487 1104 106-107 1.011433972670634 1104 108-109 0.9648358897962197 1104 110-111 0.9062920200904401 1104 112-113 0.8984101359368282 1104 114-115 0.9621566412826859 1104 116-117 0.9463759295395313 1104 118-119 0.8642542264413393 1104 120-121 0.7697172715362086 1104 122-123 0.899701563698077 1104 124-125 0.902849320251427 1105 1 0.45251489084760266 1105 2 0.32925617014486974 1105 3 0.39687698627780676 1105 4 0.3996022346875776 1105 5 0.5031069258850991 1105 6 0.45210707590758403 1105 7 0.5322444863744096 1105 8 0.5302455190243549 1105 9 0.5937144621418895 1105 10-11 0.7373212247245817 1105 12-13 0.7485431226777095 1105 14-15 0.7651987250643302 1105 16-17 0.8648309838951107 1105 18-19 0.8316260733438767 1105 20-21 0.8235177514569685 1105 22-23 0.6732028197678162 1105 24-25 0.6828517077739775 1105 26-27 0.6647492360127032 1105 28-29 0.8406188207495546 1105 30-31 0.7231336483952262 1105 32-33 0.7729614951192616 1105 34-35 0.7528208977714144 1105 36-37 0.717254699682222 1105 38-39 0.8374259466975644 1105 40-41 0.7070983443917811 1105 42-43 0.7278242149032934 1105 44-45 0.7986486506126766 1105 46-47 0.7458777070944507 1105 48-49 0.7622348387327378 1105 50-51 0.7828693293910973 1105 52-53 0.6576783892685825 1105 54-55 0.6240305274546927 1105 56-57 0.6440303026936931 1105 58-59 0.5421766993556858 1105 60-61 0.6222733186588307 1105 62-63 0.6738290165909397 1105 64-65 0.667830454601134 1105 66-67 0.6020571425698691 1105 68-69 0.5766729473105592 1105 70-71 0.6248767381640867 1105 72-73 0.581213568381866 1105 74-75 0.537167052478928 1105 76-77 0.5960488286066088 1105 78-79 0.6250191347075109 1105 80-81 0.3569674280329096 1105 82-83 0.4316648172919244 1105 84-85 0.27004717379255894 1105 86-87 0.3816084048279116 1105 88-89 0.5122293245437994 1105 90-91 0.5395287788241454 1105 92-93 0.3750926970780313 1105 94-95 0.43817333360988897 1105 96-97 0.5060031451696609 1105 98-99 0.5687106663361838 1105 100-101 0.5710232840210061 1105 102-103 0.20288814604370486 1105 104-105 0.0815507331354226 1105 106-107 0.4400508275285979 1105 108-109 0.5026919680995476 1105 110-111 0.3920633287454791 1105 112-113 0.2516652114259621 1105 114-115 0.3622129496066293 1105 116-117 0.10777338073683396 1105 118-119 -0.021877614586436778 1105 120-121 0.017184901954085063 1105 122-123 0.010054304495174193 1105 124-125 -0.07268938206422959 1106 1 0.45416607295967637 1106 2 0.4054669370255901 1106 3 0.5729028311480917 1106 4 0.402803895680357 1106 5 0.5940440140150187 1106 6 0.6762153452850086 1106 7 0.6327568728367794 1106 8 0.7487670397961352 1106 9 0.7267592204825561 1106 10-11 0.847054816606736 1106 12-13 0.7557520705142124 1106 14-15 0.8518183463626343 1106 16-17 0.9843162802057748 1106 18-19 1.024629500552038 1106 20-21 1.0285103720504978 1106 22-23 1.082944724052588 1106 24-25 1.0196368402717155 1106 26-27 1.039950563526908 1106 28-29 1.2169543227930575 1106 30-31 1.06016762664796 1106 32-33 0.9457508363804763 1106 34-35 0.9024089338345362 1106 36-37 0.9660541733025667 1106 38-39 1.0805124242466064 1106 40-41 1.1324817587871223 1106 42-43 1.029520306445967 1106 44-45 0.954644675396473 1106 46-47 0.8149230004605101 1106 48-49 0.9009946458063993 1106 50-51 1.0527703932031827 1106 52-53 1.1347903518229714 1106 54-55 1.1031553090041157 1106 56-57 0.8781077533734916 1106 58-59 0.9518095143211767 1106 60-61 1.0836269448278024 1106 62-63 1.1504895339276509 1106 64-65 1.0692469911664766 1106 66-67 0.960269589334878 1106 68-69 0.9583632822775776 1106 70-71 1.0163462516467767 1106 72-73 1.091564527213471 1106 74-75 1.1126664229609204 1106 76-77 1.103696361823058 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-1.8205463729815143 2216 112-113 -2.0183202448196553 2216 114-115 -2.0396698443438517 2216 116-117 -2.042257810985987 2216 118-119 -2.1991747304068383 2216 120-121 -2.1590547335312955 2216 122-123 -2.184839334516159 2216 124-125 -2.3302963613150354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 117041.0 3 37665.0 4 17978.0 5 13787.0 6 14620.0 7 17352.0 8 19854.0 9 21422.0 10 23367.0 11 25397.0 12 28607.0 13 30401.0 14 32456.0 15 27307.0 16 19320.0 17 16887.0 18 15525.0 19 14611.0 20 14479.0 21 15172.0 22 18116.0 23 24082.0 24 32992.0 25 44173.0 26 53510.0 27 60862.0 28 70662.0 29 83290.0 30 96685.0 31 114890.0 32 138539.0 33 172556.0 34 221394.0 35 299837.0 36 478900.0 37 738647.0 38 280276.0 39 900.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.99210915761868 20.323033170741883 13.675586598199791 25.00927107343965 2 15.274451457848148 21.234530982370096 38.44174938880229 25.04926817097946 3 19.320996108651972 25.058294935745995 29.023885215222904 26.59682374037913 4 13.36540496931785 17.435643647502353 36.3581791503864 32.8407722327934 5 13.609010208777864 38.407503641440684 33.81351015765722 14.169975992124234 6 31.993747893475593 37.46324078717704 16.874809616522796 13.668201702824568 7 28.24547901034238 32.474479942135034 20.841578453293675 18.43846259422891 8 25.738636577513223 36.799342359577466 19.06942953631312 18.392591526596185 9 25.71628697402867 16.655870977568227 19.895793850887454 37.73204819751565 10-11 24.861653236758716 27.28116613852422 27.12667089180625 20.73050973291082 12-13 25.592798872478202 24.124886302330246 28.037273433592887 22.245041391598665 14-15 22.508799167870706 25.616658454737408 25.894797439963025 25.979744937428862 16-17 22.302508633690348 28.967829119202072 26.271290171231055 22.458372075876525 18-19 22.236206204361334 27.454482041618277 28.64596461413885 21.663347139881534 20-21 22.864902088315407 26.739176894336698 28.639247379397858 21.756673637950037 22-23 22.64261097877532 26.471590310983395 28.490036493015825 22.395762217225464 24-25 22.502757300512314 26.664579930442766 28.535215411116475 22.297447357928444 26-27 22.26543690146889 27.020343361732056 28.46892148071019 22.245298256088862 28-29 22.133534229622022 26.881736939809915 28.712284145609445 22.27244468495862 30-31 22.280812344598715 26.728919355366447 28.662866335858165 22.327401964176666 32-33 22.15647617397091 26.75159069675319 28.524620164910953 22.567312964364948 34-35 22.586120578800013 26.683864384537802 28.440848990852626 22.289166045809555 36-37 22.544157752785498 26.616424725185396 28.32824027295927 22.511177249069835 38-39 22.315443286186802 26.820925891232783 28.565141061727918 22.2984897608525 40-41 22.547768837885787 26.626821780082516 28.40872271184595 22.416686670185744 42-43 22.43933380185073 26.61895491663836 28.27612527425497 22.66558600725594 44-45 22.54820317243748 26.873103948709144 27.995062484816387 22.58363039403699 46-47 22.55436277601245 27.163898796545478 27.557275713915907 22.724462713526165 48-49 22.644587202292364 27.172094502014737 27.471698736027445 22.711619559665458 50-51 22.606475506018803 27.180302022337866 27.479081322782843 22.734141148860484 52-53 22.9073975276183 27.146597597691073 27.274002639311263 22.672002235379367 54-55 22.6175229668872 27.052093217460598 27.525459967529148 22.804923848123053 56-57 22.59763868402414 27.372449614854272 27.429203665385067 22.60070803573652 58-59 22.797636929456075 26.99949255224504 27.408693788940823 22.79417672935806 60-61 22.772175022925627 26.962634777630846 27.630626840311688 22.63456335913184 62-63 22.704346443770035 27.099377772321247 27.491162015764026 22.705113768144688 64-65 22.57198737700843 26.82301737101892 27.57363858151905 23.031356670453604 66-67 22.426473675417157 27.242519962739888 27.46612986776829 22.86487649407466 68-69 22.562683208936694 27.016813055255103 27.599491130323944 22.821012605484263 70-71 22.32761913145251 26.96408255946981 27.61881294050572 23.089485368571957 72-73 22.624003585917976 27.042741165579702 27.377965425890018 22.955289822612304 74-75 22.56613388149427 27.12972424682647 27.302202093130802 23.001939778548465 76-77 22.70389028077691 26.96664367095526 27.40826267520959 22.921203373058244 78-79 22.580604996787354 27.1376405478554 27.3670139529335 22.914740502423744 80-81 22.34892615509216 27.30123325558133 27.42599069379295 22.923849895533564 82-83 22.706523363165388 27.243238563382207 27.189800889286293 22.86043718416612 84-85 22.67441616362258 27.023989031600028 27.297011849375608 23.004582955401784 86-87 22.63688006246338 26.99718419646372 27.583972809376807 22.7819629316961 88-89 22.566068800330328 26.990446666757396 27.455691360709345 22.987793172202934 90-91 22.484023003516082 27.269101237303317 27.474700736254974 22.772175022925627 92-93 22.56739396117328 27.059658689867966 27.52343761650759 22.84950973245116 94-95 22.50835157264967 26.94581543969168 27.455082923555 23.09075006410365 96-97 22.63584502636479 27.022235787054083 27.346657595346237 22.995261591234893 98-99 22.327035648018384 26.983093309365685 27.30104205998244 23.38882898263349 100-101 22.22427647449386 26.972547886357802 27.57527708517142 23.22789855397692 102-103 22.581278916756947 26.88120981405974 27.3419523734308 23.19555889575251 104-105 22.52575865084854 26.642220313396496 27.60249349328669 23.229527542468276 106-107 22.39984479300063 26.926315042700022 27.564922349445954 23.108917814853395 108-109 22.394839839540566 26.813468295623068 27.29987923700256 23.49181262783381 110-111 22.25125038257812 26.884694935027593 27.522474745500492 23.34157993689379 112-113 22.465935849130908 26.855560934986233 27.48881998584618 23.18968323003668 114-115 22.27463848079846 26.81929411206031 27.382265384237847 23.523802022903382 116-117 22.070504193954942 26.76544618367112 27.500805346487205 23.663244275886733 118-119 22.14603413421313 26.874984888582343 27.237528610347518 23.741452366857008 120-121 22.066392111840912 26.87113052097799 27.42884082466065 23.63363654252045 122-123 22.15876565023224 26.846125848150727 27.275525453136098 23.719583048480935 124-125 22.070551219041835 26.680857138472348 27.342723638196826 23.905868004288994 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 115.0 1 97.0 2 160.0 3 309.5 4 270.5 5 241.0 6 618.0 7 1278.5 8 2050.5 9 2476.5 10 2204.5 11 1832.0 12 1803.5 13 1998.5 14 2369.0 15 2728.5 16 2950.0 17 3214.0 18 3562.5 19 3973.0 20 4425.5 21 5095.5 22 6158.5 23 7434.0 24 9057.5 25 11627.0 26 15667.5 27 20842.0 28 25987.0 29 31970.0 30 38928.0 31 44681.0 32 49861.5 33 56374.5 34 63651.0 35 70718.5 36 79552.5 37 89074.0 38 96066.5 39 99754.5 40 102761.5 41 109297.5 42 116151.0 43 121702.0 44 127506.0 45 131609.5 46 131820.0 47 133973.5 48 141657.5 49 143284.0 50 138614.5 51 132932.5 52 125943.5 53 122384.0 54 118657.5 55 108886.5 56 97598.5 57 88411.0 58 79567.5 59 70793.5 60 60403.5 61 47609.0 62 36236.5 63 27136.0 64 20052.5 65 14790.5 66 10727.0 67 8248.5 68 6333.0 69 4515.5 70 3227.0 71 2362.0 72 1865.5 73 1590.5 74 1228.0 75 913.0 76 641.5 77 378.0 78 217.5 79 129.5 80 97.0 81 64.0 82 37.0 83 25.0 84 18.5 85 12.0 86 7.5 87 5.0 88 2.0 89 2.5 90 2.0 91 0.5 92 0.5 93 1.0 94 1.0 95 2.5 96 3.0 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05683991499783267 2 0.07377896251374308 3 0.0 4 0.007180997921274835 5 0.02727620984613264 6 8.686691033800205E-5 7 2.895563677933401E-5 8 0.0 9 5.791127355866803E-4 10-11 0.057809928829940356 12-13 0.009323715042945553 14-15 0.007224431376443836 16-17 0.05268478111999824 18-19 1.3030036550700308E-4 20-21 0.009989694688870236 22-23 0.01252331290706196 24-25 0.0 26-27 0.0 28-29 0.04907980434097116 30-31 0.0 32-33 0.003460198595130415 34-35 0.0 36-37 1.0134472872766905E-4 38-39 0.0 40-41 0.0 42-43 0.0032430313192854097 44-45 0.0 46-47 2.895563677933401E-5 48-49 8.686691033800205E-5 50-51 1.882116390656711E-4 52-53 1.882116390656711E-4 54-55 0.0 56-57 0.001766293843539375 58-59 4.3433455169001024E-5 60-61 0.0 62-63 0.0 64-65 5.646349171970133E-4 66-67 0.0 68-69 6.515018275350153E-4 70-71 0.0 72-73 0.0014477818389667008 74-75 0.0020848058481120492 76-77 4.777680068590113E-4 78-79 5.791127355866803E-4 80-81 0.001664949114811706 82-83 8.686691033800205E-5 84-85 4.3433455169001024E-5 86-87 4.488123700796772E-4 88-89 0.0 90-91 0.0 92-93 0.005487093169683796 94-95 0.004850069160538447 96-97 0.0053423149857871255 98-99 4.343345516900102E-4 100-101 5.212014620280123E-4 102-103 1.0134472872766905E-4 104-105 3.0403418618300717E-4 106-107 0.003083775316999073 108-109 0.0029100414963230685 110-111 4.632901884693442E-4 112-113 0.0021716727584500514 114-115 2.316450942346721E-4 116-117 0.0022151062136190522 118-119 0.0012885258366803637 120-121 0.00962774922912856 122-123 4.632901884693442E-4 124-125 0.001968983300994713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3453559.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.80812539200067 #Duplication Level Percentage of deduplicated Percentage of total 1 74.87657177751096 38.04338247794915 2 12.923878540255867 13.132760828486134 3 4.850095861727877 7.392728361176807 4 2.3583583780043633 4.79295072755684 5 1.3075499456177657 3.3217080796625553 6 0.8046045707212339 2.4528269952108794 7 0.5413495533011394 1.9253469189519492 8 0.37986914262878474 1.5440351224988067 9 0.2782366398459024 1.2723013877345588 >10 1.4951606106136004 13.82407461615852 >50 0.11489403160080962 4.039491977194711 >100 0.06489156281590107 5.963664567667207 >500 0.003505095023713247 1.2043957013518425 >1k 9.193691840520512E-4 0.7209616469137922 >5k 1.149211480065064E-4 0.36937059148626433 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7073 0.20480321894022951 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5579 0.16154349759190445 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3712 0.10748332372488786 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 2.8955636779334015E-5 0.0 0.0 2.8955636779334015E-5 0.0 9 2.8955636779334015E-5 0.0 0.0 5.791127355866803E-5 0.0 10-11 5.791127355866803E-5 0.0 0.0 5.791127355866803E-5 0.0 12-13 5.791127355866803E-5 0.0 0.0 8.686691033800205E-5 0.0 14-15 8.686691033800205E-5 0.0 0.0 8.686691033800205E-5 0.0 16-17 1.1582254711733606E-4 0.0 0.0 8.686691033800205E-5 0.0 18-19 1.1582254711733606E-4 0.0 0.0 1.0134472872766905E-4 0.0 20-21 1.1582254711733606E-4 0.0 0.0 1.4477818389667008E-4 0.0 22-23 1.1582254711733606E-4 0.0 0.0 1.882116390656711E-4 0.0 24-25 1.1582254711733606E-4 0.0 0.0 2.3164509423467212E-4 0.0 26-27 1.1582254711733606E-4 0.0 0.0 3.0403418618300717E-4 1.4477818389667007E-5 28-29 1.1582254711733606E-4 0.0 0.0 5.646349171970133E-4 2.8955636779334015E-5 30-31 1.1582254711733606E-4 0.0 0.0 0.001853160753877377 2.8955636779334015E-5 32-33 1.1582254711733606E-4 0.0 0.0 0.0045605127927451075 2.8955636779334015E-5 34-35 1.1582254711733606E-4 0.0 0.0 0.007253387013223171 2.8955636779334015E-5 36-37 1.1582254711733606E-4 0.0 0.0 0.012639135454179298 2.8955636779334015E-5 38-39 1.737338206760041E-4 0.0 0.0 0.021991806133904182 2.8955636779334015E-5 40-41 1.737338206760041E-4 0.0 0.0 0.033574060845637785 2.8955636779334015E-5 42-43 2.026894574553381E-4 0.0 0.0 0.04585125084007541 2.8955636779334015E-5 44-45 2.3164509423467212E-4 0.0 0.0 0.059112932485010386 2.8955636779334015E-5 46-47 2.6060073101400616E-4 0.0 0.0 0.0755597341756721 2.8955636779334015E-5 48-49 2.6060073101400616E-4 0.0 0.0 0.09216579186862017 2.8955636779334015E-5 50-51 2.6060073101400616E-4 0.0 0.0 0.11260847143482998 2.8955636779334015E-5 52-53 2.6060073101400616E-4 0.0 0.0 0.13448445502161682 2.8955636779334015E-5 54-55 2.6060073101400616E-4 0.0 0.0 0.15814121027033273 2.8955636779334015E-5 56-57 2.6060073101400616E-4 0.0 0.0 0.18489621865443734 2.8955636779334015E-5 58-59 2.6060073101400616E-4 0.0 0.0 0.2167329412933151 2.8955636779334015E-5 60-61 2.6060073101400616E-4 0.0 0.0 0.2516244836124126 2.8955636779334015E-5 62-63 2.6060073101400616E-4 0.0 0.0 0.2889917328761431 2.8955636779334015E-5 64-65 2.6060073101400616E-4 0.0 0.0 0.32897946726840344 2.8955636779334015E-5 66-67 2.8955636779334017E-4 0.0 0.0 0.37415026064416446 2.8955636779334015E-5 68-69 2.8955636779334017E-4 0.0 0.0 0.4264151850308624 2.8955636779334015E-5 70-71 2.8955636779334017E-4 0.0 0.0 0.48642574225603213 2.8955636779334015E-5 72-73 3.185120045726742E-4 0.0 0.0 0.5534146079450214 2.8955636779334015E-5 74-75 3.474676413520082E-4 0.0 0.0 0.6201283950846069 2.8955636779334015E-5 76-77 3.474676413520082E-4 0.0 0.0 0.693950791053519 2.8955636779334015E-5 78-79 3.474676413520082E-4 0.0 2.8955636779334015E-5 0.7810927799409247 2.8955636779334015E-5 80-81 4.053789149106762E-4 0.0 2.8955636779334015E-5 0.8842906694224711 2.8955636779334015E-5 82-83 4.343345516900102E-4 0.0 2.8955636779334015E-5 0.992686095705908 2.8955636779334015E-5 84-85 4.4881237007967723E-4 0.0 2.8955636779334015E-5 1.102210791823739 2.8955636779334015E-5 86-87 4.6329018846934423E-4 0.0 2.8955636779334015E-5 1.2232308757429653 2.8955636779334015E-5 88-89 4.6329018846934423E-4 0.0 2.8955636779334015E-5 1.3598725257046427 2.8955636779334015E-5 90-91 4.6329018846934423E-4 1.4477818389667007E-5 2.8955636779334015E-5 1.522675593496448 2.8955636779334015E-5 92-93 4.922458252486783E-4 2.8955636779334015E-5 2.8955636779334015E-5 1.6992325887584374 2.8955636779334015E-5 94-95 4.922458252486783E-4 2.8955636779334015E-5 2.8955636779334015E-5 1.8845631419645645 2.8955636779334015E-5 96-97 4.922458252486783E-4 2.8955636779334015E-5 2.8955636779334015E-5 2.0759309454391834 2.8955636779334015E-5 98-99 5.646349171970133E-4 2.8955636779334015E-5 2.8955636779334015E-5 2.2789099592623145 2.8955636779334015E-5 100-101 6.080683723660143E-4 5.791127355866803E-5 2.8955636779334015E-5 2.515665723388539 2.8955636779334015E-5 102-103 6.080683723660143E-4 5.791127355866803E-5 2.8955636779334015E-5 2.779770086452845 2.8955636779334015E-5 104-105 6.949352827040164E-4 5.791127355866803E-5 2.8955636779334015E-5 3.043613848786136 2.8955636779334015E-5 106-107 7.094131010936834E-4 5.791127355866803E-5 2.8955636779334015E-5 3.316187735608397 2.8955636779334015E-5 108-109 7.238909194833504E-4 5.791127355866803E-5 2.8955636779334015E-5 3.6069168066913004 2.8955636779334015E-5 110-111 7.238909194833504E-4 5.791127355866803E-5 2.8955636779334015E-5 3.932682198277197 2.8955636779334015E-5 112-113 7.238909194833504E-4 5.791127355866803E-5 2.8955636779334015E-5 4.298478757710524 2.8955636779334015E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6690 0.0 47.825336 1 GTATCAA 10450 0.0 44.75723 1 ATCAACG 13000 0.0 35.56228 3 TCAACGC 13175 0.0 34.7748 4 TATCAAC 13590 0.0 34.266556 2 CAACGCA 13360 0.0 33.79504 5 AACGCAG 13850 0.0 32.71941 6 GTACATG 12605 0.0 29.117075 1 TACATGG 12470 0.0 28.270823 2 ACGCAGA 16615 0.0 27.024282 7 CGCAGAG 16695 0.0 26.716438 8 ACATGGG 13290 0.0 25.742744 3 GCAGAGT 18685 0.0 22.947489 9 GAGTACT 10575 0.0 21.965914 12-13 GTACTTT 11050 0.0 20.994726 14-15 CATGGGG 6640 0.0 20.789518 4 AGTACTT 11170 0.0 20.529226 12-13 AGAGTAC 18170 0.0 20.463524 10-11 ATTCGCG 320 0.0 19.519255 80-81 CAGAGTA 18970 0.0 19.113668 10-11 >>END_MODULE