FastQCFastQC Report
Thu 26 May 2016
SRR1364632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3802142
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA90000.043.490821
TCAACGC102150.036.62514
ATCAACG102750.036.586623
CAACGCA104400.035.665525
TATCAAC112950.034.284822
AACGCAG105450.034.0929156
GTACATG147950.033.3016131
GGTATCA58150.032.887441
TACATGG147600.032.69442
ACATGGG154200.030.0586853
ACGCAGA124400.028.2722727
CGCAGAG120800.027.2370598
CATGGGA102600.024.0386754
GCAGAGT139900.022.1566879
GTACCTG33900.022.1434671
CGCATAG4700.021.5290476
CATGGGG69700.020.8552134
GTTATCA24650.020.301921
GTATAGG13800.019.8588221
GAGTACA126600.019.576471
Produced by FastQC (version 0.11.5)