FastQCFastQC Report
Thu 26 May 2016
SRR1364632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3802142
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96980.25506674921662575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76900.20225441343326997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50180.13197823753031843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82100.051.1588251
GTATCAA133800.047.0645031
ATCAACG166850.037.589693
TCAACGC167300.037.455974
CAACGCA171100.036.2091145
TATCAAC172750.036.089922
AACGCAG175100.035.2659536
GTACATG158450.029.3652291
ACGCAGA211850.028.8676037
CGCAGAG211800.028.7615768
TACATGG160050.027.903772
ACATGGG165000.026.4707643
GCAGAGT234650.025.0729319
GAGTACT138150.023.60753612-13
GTACTTT145650.022.555514-15
AGTACTT147200.022.27752512-13
GTATAGA15350.022.1228521
AGAGTAC230300.021.50158110-11
CATGGGA107650.021.2278424
CAGAGTA236300.021.0186710-11
Produced by FastQC (version 0.11.5)