##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364632_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3802142 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.88522995721885 31.0 30.0 33.0 26.0 34.0 2 29.9227154062105 31.0 30.0 34.0 26.0 34.0 3 29.946448870136887 31.0 30.0 34.0 26.0 34.0 4 33.65393770143251 35.0 35.0 37.0 28.0 37.0 5 33.13203504761263 35.0 33.0 37.0 27.0 37.0 6 33.18704009476763 35.0 33.0 37.0 27.0 37.0 7 33.004317829265716 35.0 33.0 37.0 26.0 37.0 8 32.9559153761222 35.0 33.0 37.0 26.0 37.0 9 34.258612908197534 38.0 34.0 39.0 26.0 39.0 10-11 34.08204243818353 38.0 34.0 39.0 25.0 39.0 12-13 33.964089584239616 38.0 33.5 39.0 25.0 39.0 14-15 34.98909930244583 39.0 34.0 41.0 25.0 41.0 16-17 34.91961728415193 39.0 34.0 41.0 24.5 41.0 18-19 34.962790316616264 39.0 34.0 41.0 25.0 41.0 20-21 34.98636150359455 39.0 34.0 41.0 25.0 41.0 22-23 34.983956148928684 39.0 34.0 41.0 25.0 41.0 24-25 34.989504468796795 39.0 34.0 41.0 25.0 41.0 26-27 34.9053871475605 39.0 34.0 41.0 24.5 41.0 28-29 34.68218993398984 39.0 34.0 41.0 24.0 41.0 30-31 34.62968939613512 39.0 34.0 41.0 24.0 41.0 32-33 34.47049058136177 38.0 33.0 41.0 23.5 41.0 34-35 34.35327625848798 38.0 33.0 41.0 23.0 41.0 36-37 34.20498760961584 38.0 33.0 41.0 21.5 41.0 38-39 34.08316417429964 38.0 33.0 41.0 20.0 41.0 40-41 33.92051980173281 38.0 33.0 40.5 18.5 41.0 42-43 33.76003145069279 38.0 33.0 40.0 17.0 41.0 44-45 33.58026633408221 38.0 33.0 40.0 15.0 41.0 46-47 33.3506864025594 38.0 32.5 40.0 12.5 41.0 48-49 33.124506002143 38.0 32.0 40.0 9.0 41.0 50-51 32.94442527396399 38.0 32.0 40.0 8.0 41.0 52-53 32.79009463612879 38.0 32.0 40.0 7.0 41.0 54-55 32.59133154416642 38.0 31.0 40.0 4.5 41.0 56-57 32.38357470604728 37.5 31.0 40.0 2.0 41.0 58-59 32.21760234099621 37.0 31.0 40.0 2.0 41.0 60-61 32.01480731124719 37.0 31.0 40.0 2.0 41.0 62-63 31.756431506240432 36.5 30.5 40.0 2.0 41.0 64-65 31.499721341286044 36.0 30.0 39.5 2.0 41.0 66-67 31.22806946715825 36.0 30.0 39.0 2.0 41.0 68-69 30.920278884902245 35.0 29.5 39.0 2.0 41.0 70-71 30.66247828197895 35.0 29.0 39.0 2.0 40.0 72-73 30.507532070080497 35.0 29.5 38.0 2.0 40.0 74-75 30.216508878416427 35.0 29.0 37.0 2.0 39.5 76-77 29.90370967207432 35.0 29.0 37.0 2.0 39.0 78-79 29.577355737897214 34.5 29.0 36.0 2.0 39.0 80-81 29.286264295231476 34.0 29.0 36.0 2.0 38.0 82-83 29.013335640804577 34.0 29.0 35.5 2.0 37.0 84-85 28.972936702521892 34.0 29.0 35.0 2.0 37.0 86-87 28.806976698923922 34.0 29.0 35.0 2.0 36.5 88-89 28.650074352825328 34.0 29.0 35.0 2.0 36.0 90-91 28.487771235266855 34.0 29.0 35.0 2.0 36.0 92-93 28.35099780597358 34.0 29.0 35.0 2.0 36.0 94-95 28.26169261958128 34.0 29.0 35.0 2.0 35.0 96-97 28.176976951413174 34.0 29.0 35.0 2.0 35.0 98-99 28.084394664902046 34.0 29.0 35.0 2.0 35.0 100-101 27.989780234404712 34.0 29.0 35.0 2.0 35.0 102-103 26.791493058386564 32.5 25.5 34.5 2.0 35.0 104-105 27.73880867679324 34.0 27.5 35.0 2.0 35.0 106-107 27.91441153434038 34.0 29.0 35.0 2.0 35.0 108-109 27.925120760876368 34.0 29.0 35.0 2.0 35.0 110-111 27.876880847690593 34.0 29.0 35.0 2.0 35.0 112-113 27.83224219400538 34.0 29.0 35.0 2.0 35.0 114-115 27.75773616556141 34.0 29.0 35.0 2.0 35.0 116-117 27.643697026570813 34.0 28.0 35.0 2.0 35.0 118-119 27.543159881982312 34.0 28.0 35.0 2.0 35.0 120-121 27.412400168115763 34.0 27.5 35.0 2.0 35.0 122-123 27.235636386016093 34.0 27.0 35.0 2.0 35.0 124-125 26.43220479403452 33.0 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6161043925634111 1101 2 0.5999232391799474 1101 3 0.5099884789011426 1101 4 0.5689876890905694 1101 5 0.8616434878083652 1101 6 0.9058330363012388 1101 7 0.8961620828577423 1101 8 0.8883431096443246 1101 9 0.956253998812592 1101 10-11 1.028248180027127 1101 12-13 1.0279029492111746 1101 14-15 1.1212142128248814 1101 16-17 1.2400336979429838 1101 18-19 1.2765856985847819 1101 20-21 1.302259297335759 1101 22-23 1.189262404541715 1101 24-25 1.1102777317032775 1101 26-27 1.258554274144096 1101 28-29 1.3053456385404232 1101 30-31 1.2396486965323774 1101 32-33 1.3374176487828393 1101 34-35 1.27480734093065 1101 36-37 1.3251138575786712 1101 38-39 1.3064010020737555 1101 40-41 1.3753468093869259 1101 42-43 1.2996855641814804 1101 44-45 1.227494597846082 1101 46-47 1.3927866329075798 1101 48-49 1.46906853077234 1101 50-51 1.4325330845824737 1101 52-53 1.361379465136487 1101 54-55 1.3015213290900007 1101 56-57 1.4374031299280716 1101 58-59 1.4561062092645827 1101 60-61 1.4574472013331814 1101 62-63 1.3957789007588168 1101 64-65 1.36520718627089 1101 66-67 1.3860685824510632 1101 68-69 1.439684839517568 1101 70-71 1.3286062752526462 1101 72-73 1.3568215248450421 1101 74-75 1.3466381091650348 1101 76-77 1.388623520869114 1101 78-79 1.3499444870662778 1101 80-81 1.2501709983258067 1101 82-83 1.282845870446188 1101 84-85 1.3112684914941823 1101 86-87 1.2547661518021762 1101 88-89 1.2478416016420475 1101 90-91 1.3348355454052658 1101 92-93 1.3214047173184902 1101 94-95 1.22988012590109 1101 96-97 1.2564574678856602 1101 98-99 1.3171920398310952 1101 100-101 1.2569423854095163 1101 102-103 1.2455631958236815 1101 104-105 1.2290626609812136 1101 106-107 1.2637593488328278 1101 108-109 1.297560096901119 1101 110-111 1.2892683731473298 1101 112-113 1.3028963734682506 1101 114-115 1.2629488261234592 1101 116-117 1.2931461014062293 1101 118-119 1.3197208912749332 1101 120-121 1.2521915121698868 1101 122-123 1.332594854704734 1101 124-125 1.1802996055405792 1102 1 0.29702850080592924 1102 2 -0.5791798219335966 1102 3 -0.013601623172831978 1102 4 0.2525029295452086 1102 5 0.585284820366077 1102 6 0.5082616315110045 1102 7 0.4155582879934485 1102 8 0.39902503406769085 1102 9 0.6633019066861721 1102 10-11 0.8139749004100381 1102 12-13 0.8399738248040265 1102 14-15 0.9998784977450015 1102 16-17 1.0998973781713914 1102 18-19 0.9802849203605604 1102 20-21 0.9296035987430074 1102 22-23 0.9129425131699946 1102 24-25 0.9674620852746898 1102 26-27 0.9154718637068342 1102 28-29 0.993219601107775 1102 30-31 0.8992416660391882 1102 32-33 0.8955730473025625 1102 34-35 0.7939980712844701 1102 36-37 0.8119270604237485 1102 38-39 0.7108751309866008 1102 40-41 0.7175688671201215 1102 42-43 0.5422919580406997 1102 44-45 0.4529253270450653 1102 46-47 0.49871365895342734 1102 48-49 0.5491153016240489 1102 50-51 0.5334958368265674 1102 52-53 0.6859753800889763 1102 54-55 0.6607376101868709 1102 56-57 0.5123864984490893 1102 58-59 0.5978777120930019 1102 60-61 0.4355934379504447 1102 62-63 0.5807429534217867 1102 64-65 0.5148035619291598 1102 66-67 0.49114669665327426 1102 68-69 0.34641522896138355 1102 70-71 0.19856150079269952 1102 72-73 0.23975828775417085 1102 74-75 0.36854136770636714 1102 76-77 0.46439193205456064 1102 78-79 0.5888876556286959 1102 80-81 0.39123779022254723 1102 82-83 0.3510604266605881 1102 84-85 0.45775427380639755 1102 86-87 0.4011348687734113 1102 88-89 0.5145537502079982 1102 90-91 0.4600887339500339 1102 92-93 0.5704796047373506 1102 94-95 0.3082485148498044 1102 96-97 0.3623651135746506 1102 98-99 0.4257494655791447 1102 100-101 0.20947103055979 1102 102-103 0.20275450605251777 1102 104-105 0.27082607074461507 1102 106-107 0.5055783657849595 1102 108-109 0.5739307273370642 1102 110-111 0.45323044600396045 1102 112-113 0.49232474201076926 1102 114-115 0.6030055820052915 1102 116-117 0.5748767519166584 1102 118-119 0.5688080948952177 1102 120-121 0.6064494164991032 1102 122-123 0.5758007342934413 1102 124-125 0.4015589970280864 1103 1 0.20313292939945882 1103 2 -1.1126336737289435 1103 3 -0.034432734061780934 1103 4 0.20493516176538407 1103 5 0.48060582667288543 1103 6 0.5697630614580973 1103 7 0.37839413253691845 1103 8 0.544510429797711 1103 9 0.6085954890297387 1103 10-11 0.7772613249261227 1103 12-13 0.7776850375768447 1103 14-15 0.7920791188559022 1103 16-17 0.8536506755096838 1103 18-19 0.9139355187062534 1103 20-21 0.8565188536293178 1103 22-23 0.8782188082354381 1103 24-25 0.7458042266339149 1103 26-27 0.8424492352965345 1103 28-29 0.9161549277968888 1103 30-31 0.8219593348018037 1103 32-33 0.7432923586341289 1103 34-35 0.9192774210448178 1103 36-37 0.9987601341631063 1103 38-39 0.837076626907745 1103 40-41 0.8545761124161473 1103 42-43 0.867832098169373 1103 44-45 0.9119000353499231 1103 46-47 0.8088415130280708 1103 48-49 0.7603596629038947 1103 50-51 0.7920740615729684 1103 52-53 0.8556492446325876 1103 54-55 0.8249532344773627 1103 56-57 0.7975699589787411 1103 58-59 0.855970229122633 1103 60-61 0.8236955291223609 1103 62-63 0.8889182475071173 1103 64-65 0.8540607197488406 1103 66-67 0.7947256353363876 1103 68-69 0.7316429414055783 1103 70-71 0.7997587316475254 1103 72-73 0.8210575926925827 1103 74-75 0.697461177256308 1103 76-77 0.6758570383862299 1103 78-79 0.7421914612839942 1103 80-81 0.8056123313049959 1103 82-83 0.793330731050208 1103 84-85 0.7672580452289033 1103 86-87 0.7058315651034022 1103 88-89 0.7100303850505547 1103 90-91 0.7452733014791662 1103 92-93 0.725709417985918 1103 94-95 0.6019984986735771 1103 96-97 0.5933292985865073 1103 98-99 0.632457888682076 1103 100-101 0.6198163118061046 1103 102-103 0.6685653103612346 1103 104-105 0.7166569688090156 1103 106-107 0.6779820754618413 1103 108-109 0.6656959639921958 1103 110-111 0.7019748201468339 1103 112-113 0.7192058763665479 1103 114-115 0.7044887461490852 1103 116-117 0.6921903049050897 1103 118-119 0.7186161988735904 1103 120-121 0.7282147289291316 1103 122-123 0.7919465930442371 1103 124-125 0.7082206486232927 1104 1 0.2089590548933309 1104 2 -0.8355578215685071 1104 3 -0.004732827898560998 1104 4 0.13396712415603673 1104 5 0.47879052007917267 1104 6 0.49918876939207735 1104 7 0.5327292394668959 1104 8 0.6352819387348916 1104 9 0.5282911475633298 1104 10-11 0.6904917597876263 1104 12-13 0.593269591592545 1104 14-15 0.612629807089732 1104 16-17 0.7730752496721607 1104 18-19 0.693170171149248 1104 20-21 0.7152078369658454 1104 22-23 0.6574984117043812 1104 24-25 0.6342434285690359 1104 26-27 0.4842418852403867 1104 28-29 0.7871322161092849 1104 30-31 0.6766408160423083 1104 32-33 0.6676514589314735 1104 34-35 0.7205651407183424 1104 36-37 0.6912118633796922 1104 38-39 0.713971093802968 1104 40-41 0.6924411240611548 1104 42-43 0.5627259493809262 1104 44-45 0.5881283516671445 1104 46-47 0.6803472939450614 1104 48-49 0.7001555634369723 1104 50-51 0.6606075467704926 1104 52-53 0.41465920373836695 1104 54-55 0.7020867081584612 1104 56-57 0.7133441554359905 1104 58-59 0.635930928476995 1104 60-61 0.7005420613824356 1104 62-63 0.6864058940776765 1104 64-65 0.7424574847652572 1104 66-67 0.7085365715029894 1104 68-69 0.6542695340508686 1104 70-71 0.709954926821851 1104 72-73 0.6416664688784692 1104 74-75 0.6693711630085879 1104 76-77 0.6567341782966274 1104 78-79 0.6259915295404888 1104 80-81 0.5116312130985499 1104 82-83 0.5825148266139095 1104 84-85 0.5996029406385581 1104 86-87 0.604431289151357 1104 88-89 0.6065576820305658 1104 90-91 0.6493778450858585 1104 92-93 0.6995854053144512 1104 94-95 0.6167583073797012 1104 96-97 0.6002277665471034 1104 98-99 0.7349674765935461 1104 100-101 0.7448733525074083 1104 102-103 0.6847741384095372 1104 104-105 0.674890392883448 1104 106-107 0.7596826854539245 1104 108-109 0.673067462745724 1104 110-111 0.5737997563771096 1104 112-113 0.5323583496710427 1104 114-115 0.6383434174830249 1104 116-117 0.6253646679052842 1104 118-119 0.5849067066314788 1104 120-121 0.5513820121427351 1104 122-123 0.5531417990233756 1104 124-125 0.46284805232563997 1105 1 0.43725832008710697 1105 2 0.2410818445658336 1105 3 0.3553667980676849 1105 4 0.4270674619204158 1105 5 0.5829522079076597 1105 6 0.5260592661793808 1105 7 0.5864234319626007 1105 8 0.5988051837002928 1105 9 0.6894337932687336 1105 10-11 0.7231258621581631 1105 12-13 0.6305703297023726 1105 14-15 0.6742360177744828 1105 16-17 0.8267994616333709 1105 18-19 0.8323415846988098 1105 20-21 0.8888973013225225 1105 22-23 0.7674943606873441 1105 24-25 0.8535962047926375 1105 26-27 0.8701804659411465 1105 28-29 0.9437589970104128 1105 30-31 0.8315632820596903 1105 32-33 0.8773632421896451 1105 34-35 0.8589887620604841 1105 36-37 0.8993884951031248 1105 38-39 0.8683949584638526 1105 40-41 0.830752384740471 1105 42-43 0.8884663934089474 1105 44-45 0.9986977134433417 1105 46-47 1.050216030098042 1105 48-49 1.041257817292582 1105 50-51 0.9642006249202169 1105 52-53 0.8717857692022335 1105 54-55 0.8537765170507328 1105 56-57 0.9019220153275 1105 58-59 0.7591783188628938 1105 60-61 0.8939582683559024 1105 62-63 0.9607537014207672 1105 64-65 0.885309123705067 1105 66-67 0.8787219881227948 1105 68-69 0.8666565349252693 1105 70-71 0.8992862460101279 1105 72-73 0.81328614234166 1105 74-75 0.8171949040537072 1105 76-77 0.9139208236936973 1105 78-79 0.8935707235263379 1105 80-81 0.6635536215957032 1105 82-83 0.7409790038146227 1105 84-85 0.6087429160466122 1105 86-87 0.7683797298260977 1105 88-89 0.8067974745922548 1105 90-91 0.7728837250351752 1105 92-93 0.6287250427472557 1105 94-95 0.759438103184074 1105 96-97 0.7912435438409773 1105 98-99 0.8293435508511031 1105 100-101 0.7752245404230536 1105 102-103 0.4514440208199204 1105 104-105 0.4368612331754349 1105 106-107 0.7724730425104269 1105 108-109 0.7616910605877472 1105 110-111 0.6720114104197847 1105 112-113 0.5243297973812986 1105 114-115 0.7727424242692393 1105 116-117 0.5442306484763364 1105 118-119 0.4608800255648404 1105 120-121 0.4674832438721559 1105 122-123 0.6036884871705119 1105 124-125 0.4868180537126854 1106 1 0.43193252687741435 1106 2 0.38438460336145397 1106 3 0.48555376093062463 1106 4 0.3361747326673381 1106 5 0.49729633185203426 1106 6 0.6221368601037582 1106 7 0.5846779696517217 1106 8 0.6766600998099292 1106 9 0.6715908881405639 1106 10-11 0.8280605641030476 1106 12-13 0.6426749721145697 1106 14-15 0.8455577945791148 1106 16-17 0.956174307765977 1106 18-19 0.8597696120629621 1106 20-21 0.9730821119071038 1106 22-23 0.841611581525278 1106 24-25 0.8714524567509514 1106 26-27 0.901494868767827 1106 28-29 1.0808148564670077 1106 30-31 0.9655556651577726 1106 32-33 0.8992989615243374 1106 34-35 0.7857533645417547 1106 36-37 0.9681268268262855 1106 38-39 0.9873870781223246 1106 40-41 0.9696179927684341 1106 42-43 0.9149712747352297 1106 44-45 0.9452150999917919 1106 46-47 0.8619923288320379 1106 48-49 1.017478545187899 1106 50-51 1.015377092628583 1106 52-53 1.0156936814362751 1106 54-55 1.0082382166605655 1106 56-57 0.8853335956667863 1106 58-59 0.8560996189583889 1106 60-61 0.9433811724023258 1106 62-63 0.9645043355409051 1106 64-65 0.9107619950570118 1106 66-67 0.8389106668402384 1106 68-69 0.8970465677689603 1106 70-71 0.9222662819498169 1106 72-73 0.9778494107460602 1106 74-75 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-2.0721684625186576 2216 110-111 -1.979903034199257 2216 112-113 -2.072676906691271 2216 114-115 -2.113480220071292 2216 116-117 -2.0948614445832554 2216 118-119 -2.240520443544142 2216 120-121 -2.263307090062632 2216 122-123 -2.174698950155193 2216 124-125 -2.1957946606803667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 119462.0 3 37507.0 4 17446.0 5 13756.0 6 14823.0 7 17779.0 8 20737.0 9 22595.0 10 24595.0 11 27702.0 12 31231.0 13 34830.0 14 37966.0 15 31736.0 16 21775.0 17 18861.0 18 17126.0 19 15864.0 20 15553.0 21 16485.0 22 19110.0 23 25612.0 24 34876.0 25 46712.0 26 58104.0 27 65699.0 28 75610.0 29 88991.0 30 104094.0 31 123018.0 32 149150.0 33 185472.0 34 238565.0 35 323822.0 36 509799.0 37 834253.0 38 360046.0 39 1380.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.992707384013826 20.610173345358305 13.85810202504243 24.53901724558544 2 14.950151859359204 21.50114799305861 39.09103902123557 24.45766112634662 3 18.96507284578009 25.667689423488127 29.38030720578032 25.98693052495146 4 12.808382436569104 17.79421303395659 37.30950741401432 32.08789711545998 5 13.002908548448842 38.90993234434793 34.35391680577974 13.733242301423493 6 31.075929624636323 38.20257403358016 17.305212925610565 13.416283416172952 7 27.44775310746054 33.0168799675972 21.360049866654045 18.175317058288226 8 25.434275057132282 37.02782195610315 19.416718106982355 18.121184879782206 9 25.74663687273087 16.604490762512246 20.229761532788583 37.419110831968304 10-11 24.330685100869946 27.724385923498374 27.551960015716233 20.39296895991545 12-13 25.213467145182385 24.76318837926474 28.355436277341312 21.667908198211563 14-15 22.13832879935347 26.13914682269607 26.426691233669946 25.29583314428051 16-17 21.59217329096331 29.424053361915686 26.9884962250792 21.995277122041802 18-19 21.482603072956078 27.981066963131525 29.43213250863922 21.10419745527318 20-21 22.314800116315666 27.07086667402298 29.533618107681953 21.080715101979404 22-23 21.89697701839541 26.983850995396054 29.326742296555885 21.792429689652657 24-25 21.661815892199712 27.09829091075504 29.550579646946378 21.689313550098866 26-27 21.639538975661615 27.37814631857516 29.396008881309537 21.58630582445369 28-29 21.47881880279017 27.2387308057427 29.58553364327907 21.696916748188055 30-31 21.725700933842027 27.105313268152532 29.590767519992678 21.578218278012763 32-33 21.60010447145453 27.12203367941914 29.45023212152671 21.827629727599625 34-35 21.799264730249423 27.162294306735518 29.39499629419417 21.64344466882089 36-37 21.835963482564637 27.05192275170043 29.315220498831053 21.796893266903886 38-39 21.70444975490132 27.308843278341527 29.328928272536903 21.65777869422026 40-41 21.92357911981971 27.137193605235367 29.235958209340712 21.703269065604214 42-43 21.842055600962915 27.025705532808043 29.12976779999487 22.002471066234175 44-45 21.909189609435945 27.30072943093656 28.887466591200432 21.90261436842706 46-47 21.924490651773993 27.48625543230474 28.443124836194155 22.146129079727107 48-49 22.027337308298243 27.528661566016943 28.340114619963018 22.103886505721793 50-51 22.133235142305757 27.649813097324184 28.09460530165796 22.122346458712098 52-53 22.246172216399472 27.71571761654965 27.953662876252423 22.084447290798458 54-55 22.07859702223076 27.599748720556562 28.107515199000353 22.214139058212325 56-57 22.011714391258145 27.807127975233527 28.0916041777708 22.08955345573753 58-59 22.16253364550824 27.57140580230828 28.118439553283388 22.147620998900095 60-61 22.18291689263578 27.589395661708583 28.232283276111204 21.99540416954443 62-63 22.04225933697374 27.64703422439246 28.22645761257733 22.08424882605647 64-65 22.017867963910582 27.483869491482626 28.26163458625101 22.236627958355776 66-67 21.946905191862903 27.680344395343464 28.138743897518818 22.23400651527481 68-69 21.933184986446395 27.532791206600415 28.21851309708559 22.3155107098676 70-71 21.888188289653566 27.43466709028753 28.2418305260561 22.435314094002802 72-73 22.124225368449306 27.525193977616546 27.995887481212435 22.354693172721714 74-75 21.96882262647857 27.700468807371305 27.918657291922017 22.412051274228116 76-77 22.114313352943405 27.507595746382023 28.02284667954367 22.355244221130903 78-79 22.070088579925372 27.621319043697657 27.989154949247823 22.319437427129145 80-81 21.893431984870347 27.801148821714065 27.97351547624261 22.331903717172978 82-83 22.111348930522777 27.739761155434127 27.83514170185301 22.313748212190088 84-85 22.10577893432036 27.518853213337657 27.87984756993238 22.495520282409604 86-87 21.97143672853083 27.488673754553545 28.166545484289625 22.373344032626004 88-89 22.099769301063624 27.47130794579844 28.049127051163143 22.379795701974796 90-91 22.068507699081202 27.662591244619477 27.982818632234146 22.28608242406517 92-93 22.043869134340543 27.520619550519182 28.04704705836864 22.38846425677163 94-95 21.932441330909803 27.538092512011442 28.000151503375093 22.529314653703658 96-97 22.081280959857168 27.503295063861504 27.949479983555587 22.46594399272574 98-99 21.965487357594583 27.44216660069409 27.837949440898875 22.75439660081245 100-101 21.80448804228056 27.520882727306084 27.984360326875056 22.690268903538296 102-103 21.928558653810747 27.42619945609578 27.937977033986122 22.70726485610735 104-105 21.93359426626126 27.345144881423593 28.00211934032179 22.719141511993353 106-107 21.82987417736055 27.460211749846593 27.974962249525838 22.73495182326702 108-109 21.8715814906864 27.38043979438772 27.76399670376466 22.98398201116122 110-111 21.721922008486874 27.552453824275286 27.8507227310338 22.87490143620404 112-113 21.753746363907396 27.434539812279713 27.921956061653557 22.889757762159334 114-115 21.664592607477054 27.404789768249366 27.85331520487685 23.07730241939673 116-117 21.534203311028268 27.442855521551984 27.896636855129398 23.12630431229035 118-119 21.61943821383182 27.40860375127301 27.752723818100712 23.21923421679446 120-121 21.539862370618163 27.475252766180645 27.820897933007704 23.163986930193488 122-123 21.564497654594877 27.459414036661173 27.71574505242477 23.260343256319178 124-125 21.498433747932047 27.369564474089113 27.71678322597953 23.415218551999306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 103.0 1 86.5 2 165.0 3 334.5 4 295.5 5 286.0 6 768.0 7 1569.5 8 2480.0 9 3030.0 10 2722.0 11 2266.0 12 2304.5 13 2579.5 14 2969.5 15 3391.0 16 3731.0 17 4131.5 18 4595.0 19 5018.5 20 5538.0 21 6526.0 22 7838.0 23 9690.5 24 12200.0 25 15429.5 26 20265.5 27 26363.0 28 32068.5 29 39105.5 30 48493.0 31 56083.0 32 62851.0 33 72530.0 34 83784.5 35 93646.5 36 104264.5 37 114723.0 38 120964.0 39 124803.5 40 128525.0 41 133729.5 42 138172.0 43 141785.0 44 145716.5 45 149377.0 46 149293.5 47 147209.0 48 149181.0 49 147227.0 50 141441.0 51 135187.5 52 127764.0 53 120947.5 54 112927.5 55 103267.0 56 91477.5 57 80324.5 58 72229.0 59 64506.0 60 54600.0 61 42808.5 62 31933.0 63 23851.0 64 17964.0 65 13229.0 66 9549.5 67 7242.5 68 5389.0 69 3771.0 70 2730.5 71 2128.0 72 1748.5 73 1465.0 74 1149.0 75 845.0 76 570.0 77 328.0 78 194.5 79 127.0 80 80.5 81 59.5 82 34.5 83 24.5 84 25.0 85 18.5 86 15.0 87 11.0 88 7.5 89 5.5 90 1.0 91 1.0 92 1.0 93 1.5 94 1.5 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05972948932470171 2 0.0763516985951603 3 0.0 4 0.007574677642234298 5 0.03358633107337917 6 4.2081542456857214E-4 7 5.260192807107152E-5 8 2.630096403553576E-5 9 6.838250649239297E-4 10-11 0.059150868115919915 12-13 0.010152172117716803 14-15 0.007745633908465281 16-17 0.05598160194963787 18-19 1.315048201776788E-4 20-21 0.011006953448871716 22-23 0.013531845996283149 24-25 0.0 26-27 0.0 28-29 0.05298329204958678 30-31 0.0 32-33 0.0038530912312059887 34-35 0.0 36-37 3.4191253246196486E-4 38-39 0.0 40-41 1.315048201776788E-5 42-43 0.003668984482957238 44-45 0.0 46-47 9.205337412437515E-5 48-49 3.156115684264291E-4 50-51 7.758784390483048E-4 52-53 1.841067482487503E-4 54-55 1.315048201776788E-5 56-57 0.0017490141083631278 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.890289210660727E-4 66-67 0.0 68-69 4.602668706218758E-4 70-71 0.0 72-73 0.0024328391732870575 74-75 0.0040503484614725065 76-77 3.682134964975006E-4 78-79 0.0010257375973858945 80-81 0.0019068198925763426 82-83 1.0520385614214304E-4 84-85 1.315048201776788E-4 86-87 5.654707267640187E-4 88-89 1.315048201776788E-5 90-91 0.0 92-93 0.007600978606269834 94-95 0.006483187634759565 96-97 0.006391134260635189 98-99 0.0014991549500255382 100-101 4.3396590658634004E-4 102-103 5.260192807107152E-5 104-105 4.076649425508043E-4 106-107 0.005339095699213759 108-109 0.006022920764137689 110-111 9.468347052792873E-4 112-113 0.0040897999075258104 114-115 4.3396590658634004E-4 116-117 0.0023802372452159863 118-119 0.003313921468477505 120-121 0.014610185521740114 122-123 7.101260289594655E-4 124-125 0.0018936694105585745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3802142.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.34717001791101 #Duplication Level Percentage of deduplicated Percentage of total 1 73.1656396501893 36.836828989372925 2 13.195156726202569 13.286775982142057 3 5.3325368909306645 8.054344244234064 4 2.6495033684423275 5.335799862159753 5 1.4841464255518744 3.7361286209367566 6 0.9290478883410243 2.8064959193452057 7 0.6484916050125464 2.2854801964928275 8 0.4545214981128442 1.830709691382639 9 0.3320894240642542 1.504778642506184 >10 1.6460076239492731 14.72576358700281 >50 0.10893273032295266 3.7720018190725817 >100 0.0520866605159391 4.570130986450261 >500 0.0014190493214538637 0.4571958266078091 >1k 2.6278690187433816E-4 0.2046770235159699 >5k 1.576721411246019E-4 0.5928886087781257 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9698 0.25506674921662575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7690 0.20225441343326997 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5018 0.13197823753031843 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.630096403553576E-5 0.0 5 0.0 0.0 0.0 2.630096403553576E-5 0.0 6 0.0 0.0 0.0 2.630096403553576E-5 0.0 7 0.0 0.0 0.0 2.630096403553576E-5 0.0 8 0.0 0.0 0.0 2.630096403553576E-5 0.0 9 5.260192807107152E-5 0.0 0.0 2.630096403553576E-5 0.0 10-11 5.260192807107152E-5 0.0 0.0 7.890289210660728E-5 0.0 12-13 1.0520385614214304E-4 0.0 0.0 7.890289210660728E-5 0.0 14-15 1.5780578421321456E-4 0.0 0.0 9.205337412437515E-5 0.0 16-17 1.7095626623098243E-4 0.0 0.0 1.315048201776788E-4 0.0 18-19 1.841067482487503E-4 0.0 0.0 1.315048201776788E-4 0.0 20-21 1.841067482487503E-4 0.0 0.0 1.315048201776788E-4 0.0 22-23 1.841067482487503E-4 0.0 0.0 2.2355819430205394E-4 0.0 24-25 2.1040771228428607E-4 0.0 0.0 3.4191253246196486E-4 0.0 26-27 2.1040771228428607E-4 0.0 0.0 4.865678346574115E-4 0.0 28-29 2.1040771228428607E-4 0.0 0.0 7.364269929950012E-4 0.0 30-31 2.3670867631982184E-4 0.0 0.0 0.0021172276048606283 0.0 32-33 2.3670867631982184E-4 0.0 0.0 0.0049971831667517935 0.0 34-35 2.4985915833758974E-4 0.0 0.0 0.00874507054181564 0.0 36-37 2.893106043908933E-4 0.0 0.0 0.015294010586664044 0.0 38-39 3.4191253246196486E-4 0.0 0.0 0.027247798740815045 0.0 40-41 3.550630144797327E-4 0.0 0.0 0.040490334132707306 0.0 42-43 4.2081542456857214E-4 0.0 0.0 0.053943277236883844 0.0 44-45 4.471163886041079E-4 0.0 0.0 0.06938194312574333 0.0 46-47 5.917716907995545E-4 0.0 0.0 0.08796357421684933 0.0 48-49 6.049221728173224E-4 0.0 0.0 0.10892544255317134 0.0 50-51 6.049221728173224E-4 0.0 0.0 0.13356944585446834 0.0 52-53 6.049221728173224E-4 0.0 0.0 0.15829235204787195 0.0 54-55 6.049221728173224E-4 0.0 0.0 0.18480372379569202 1.315048201776788E-5 56-57 6.049221728173224E-4 0.0 0.0 0.21624652630017502 2.630096403553576E-5 58-59 6.049221728173224E-4 0.0 0.0 0.252844317755623 2.630096403553576E-5 60-61 6.049221728173224E-4 0.0 0.0 0.29442614189580507 2.630096403553576E-5 62-63 6.049221728173224E-4 0.0 0.0 0.3361657718202003 2.630096403553576E-5 64-65 6.049221728173224E-4 0.0 0.0 0.380482896220078 2.630096403553576E-5 66-67 6.312231368528582E-4 0.0 0.0 0.4325325040464033 2.630096403553576E-5 68-69 6.312231368528582E-4 0.0 0.0 0.49465538109833873 2.630096403553576E-5 70-71 6.57524100888394E-4 0.0 0.0 0.5688240996785496 2.630096403553576E-5 72-73 6.838250649239297E-4 0.0 0.0 0.6443999198346616 2.630096403553576E-5 74-75 7.364269929950012E-4 0.0 0.0 0.7207779193938575 2.630096403553576E-5 76-77 7.364269929950012E-4 0.0 0.0 0.8072686396247168 2.630096403553576E-5 78-79 7.364269929950012E-4 0.0 0.0 0.9111311466010475 2.630096403553576E-5 80-81 9.994366333503587E-4 0.0 0.0 1.0296958924732427 2.630096403553576E-5 82-83 0.0010520385614214303 0.0 0.0 1.1581498008227993 2.630096403553576E-5 84-85 0.0010783395254569661 0.0 0.0 1.2889444949715188 2.630096403553576E-5 86-87 0.0010783395254569661 0.0 0.0 1.4281028956835384 2.630096403553576E-5 88-89 0.001091490007474734 0.0 0.0 1.588565077264342 2.630096403553576E-5 90-91 0.001183543381599109 0.0 0.0 1.7713436268292977 2.630096403553576E-5 92-93 0.0012361453096701807 0.0 0.0 1.9729536666436973 2.630096403553576E-5 94-95 0.0012361453096701807 0.0 0.0 2.1788770645599245 2.630096403553576E-5 96-97 0.0012361453096701807 0.0 0.0 2.3921647324061017 2.630096403553576E-5 98-99 0.0013808006118656273 0.0 0.0 2.6306355733163045 2.630096403553576E-5 100-101 0.0015123054320433061 0.0 0.0 2.9033502694007747 2.630096403553576E-5 102-103 0.001617509288185449 0.0 0.0 3.2029445507295624 2.630096403553576E-5 104-105 0.0019199703745941103 0.0 0.0 3.5059316564189347 2.630096403553576E-5 106-107 0.002038324712754021 0.0 0.0 3.811864470080286 2.630096403553576E-5 108-109 0.002143528568896164 0.0 0.0 4.140468714740271 2.630096403553576E-5 110-111 0.002209280978985004 0.0 0.0 4.503895435783303 2.630096403553576E-5 112-113 0.002209280978985004 0.0 0.0 4.909403699283194 2.630096403553576E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8210 0.0 51.158825 1 GTATCAA 13380 0.0 47.064503 1 ATCAACG 16685 0.0 37.58969 3 TCAACGC 16730 0.0 37.45597 4 CAACGCA 17110 0.0 36.209114 5 TATCAAC 17275 0.0 36.08992 2 AACGCAG 17510 0.0 35.265953 6 GTACATG 15845 0.0 29.365229 1 ACGCAGA 21185 0.0 28.867603 7 CGCAGAG 21180 0.0 28.761576 8 TACATGG 16005 0.0 27.90377 2 ACATGGG 16500 0.0 26.470764 3 GCAGAGT 23465 0.0 25.072931 9 GAGTACT 13815 0.0 23.607536 12-13 GTACTTT 14565 0.0 22.5555 14-15 AGTACTT 14720 0.0 22.277525 12-13 GTATAGA 1535 0.0 22.122852 1 AGAGTAC 23030 0.0 21.501581 10-11 CATGGGA 10765 0.0 21.227842 4 CAGAGTA 23630 0.0 21.01867 10-11 >>END_MODULE