FastQCFastQC Report
Thu 26 May 2016
SRR1364631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928122
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58400.048.5592771
ATCAACG67200.040.426513
GGTATCA31300.039.7814031
TCAACGC67600.039.7480134
CAACGCA67800.039.281295
AACGCAG68200.037.1831636
TATCAAC74800.037.1154752
GTACATG112000.035.1078341
TACATGG112200.032.973832
ACATGGG116100.030.7885823
ACGCAGA85250.029.1844147
CGCAGAG84900.027.4052688
CGCATAG3850.023.1843818
CATGGGA72250.023.0065734
CATGGGG55400.022.691244
GCAGAGT96500.022.5677919
GTACCTG28950.022.0197981
GAGTACA88350.021.5110821
TACCTGG29900.020.7220552
GTGTAGC15650.020.1762141
Produced by FastQC (version 0.11.5)