##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364631_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2928122 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.973446803104515 31.0 26.0 31.0 7.0 34.0 2 25.82259345751304 31.0 25.0 31.0 7.0 34.0 3 26.22727775686942 31.0 26.0 31.0 7.0 34.0 4 29.48549172473005 35.0 28.0 37.0 7.0 37.0 5 29.813763907378178 35.0 30.0 37.0 2.0 37.0 6 29.786682043985873 35.0 30.0 37.0 2.0 37.0 7 29.660900058125993 35.0 30.0 37.0 2.0 37.0 8 29.417537930455083 35.0 29.0 37.0 2.0 37.0 9 30.29540060147767 37.0 28.0 39.0 2.0 39.0 10-11 30.227225675706137 36.0 28.0 39.0 2.0 39.0 12-13 30.224599931287017 37.0 28.0 39.0 2.0 39.0 14-15 30.824766864222187 37.0 27.5 40.0 2.0 41.0 16-17 31.19570718023361 37.0 29.0 40.0 2.0 41.0 18-19 30.991774249843417 37.0 28.0 40.0 2.0 41.0 20-21 30.905455100572993 37.0 27.5 40.0 2.0 41.0 22-23 30.948134162442685 37.0 28.0 40.0 2.0 41.0 24-25 31.021719211153087 37.0 28.5 40.0 2.0 41.0 26-27 30.825131261607268 37.0 27.5 40.0 2.0 41.0 28-29 30.709179467248973 37.0 28.0 40.0 2.0 41.0 30-31 30.57133975292013 37.0 27.5 40.0 2.0 41.0 32-33 30.487415654129165 37.0 27.0 40.0 2.0 41.0 34-35 30.535469492049852 37.0 28.0 40.0 2.0 41.0 36-37 30.470287952482856 37.0 28.0 40.0 2.0 41.0 38-39 30.188364590000006 37.0 27.0 40.0 2.0 41.0 40-41 30.05775305810345 37.0 27.0 40.0 2.0 41.0 42-43 29.555259138792714 36.0 25.5 40.0 2.0 41.0 44-45 29.40758154885623 36.0 25.0 40.0 2.0 41.0 46-47 29.372360168053106 35.5 25.5 40.0 2.0 41.0 48-49 29.31440117590729 36.0 26.0 40.0 2.0 41.0 50-51 29.078660144625122 35.0 25.0 40.0 2.0 41.0 52-53 28.977272463374135 35.0 25.0 40.0 2.0 41.0 54-55 28.802867503471507 35.0 24.0 39.0 2.0 41.0 56-57 28.579561575644732 35.0 24.0 39.0 2.0 41.0 58-59 28.372412419974303 35.0 23.5 39.0 2.0 40.0 60-61 28.07717950276662 34.0 23.0 39.0 2.0 40.0 62-63 27.89496595428742 34.0 22.5 39.0 2.0 40.0 64-65 27.67766319162931 34.0 22.5 38.0 2.0 40.0 66-67 27.421309631224382 34.0 22.0 38.0 2.0 40.0 68-69 27.195908674570255 34.0 22.0 37.5 2.0 40.0 70-71 26.768533210023353 33.0 20.5 37.0 2.0 39.5 72-73 26.535974764712673 33.0 20.0 36.5 2.0 39.0 74-75 26.51359625725977 33.0 21.0 36.0 2.0 39.0 76-77 26.27333884995229 33.0 20.0 36.0 2.0 39.0 78-79 25.979827172501693 33.0 20.0 35.0 2.0 37.5 80-81 25.575164217884364 32.0 18.0 35.0 2.0 37.0 82-83 25.6880673004745 33.0 20.0 35.0 2.0 37.0 84-85 25.527749526829822 33.0 18.5 35.0 2.0 36.0 86-87 25.40024305681252 33.0 18.0 35.0 2.0 36.0 88-89 25.238190382777766 33.0 18.0 35.0 2.0 36.0 90-91 25.110263677538025 33.0 17.5 35.0 2.0 35.0 92-93 24.983536546632962 32.5 15.5 35.0 2.0 35.0 94-95 24.882011576020396 32.0 13.0 35.0 2.0 35.0 96-97 24.773001090801543 32.0 10.5 35.0 2.0 35.0 98-99 24.68102951311455 32.0 8.5 35.0 2.0 35.0 100-101 24.578031243233717 32.0 7.0 35.0 2.0 35.0 102-103 23.461847047356635 30.0 7.0 34.0 2.0 35.0 104-105 24.290071417789285 32.0 3.5 34.5 2.0 35.0 106-107 24.448966436507767 32.0 2.0 35.0 2.0 35.0 108-109 24.412965204318674 32.0 2.0 35.0 2.0 35.0 110-111 24.34975950455616 32.0 2.0 35.0 2.0 35.0 112-113 24.26073350085823 32.0 2.0 35.0 2.0 35.0 114-115 24.162158373182535 32.0 2.0 35.0 2.0 35.0 116-117 24.047654605921473 32.0 2.0 35.0 2.0 35.0 118-119 23.921633900500048 32.0 2.0 35.0 2.0 35.0 120-121 23.73573898218722 32.0 2.0 35.0 2.0 35.0 122-123 23.51962862203146 32.0 2.0 35.0 2.0 35.0 124-125 22.584912957861725 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.3870335957627624 1101 2 1.6662999792770634 1101 3 1.166063445672659 1101 4 1.2878858703329144 1101 5 1.1418168424865094 1101 6 1.0341757106812963 1101 7 1.0284526237857747 1101 8 1.3297192077856188 1101 9 1.7466655594596645 1101 10-11 1.651468189307252 1101 12-13 1.700818665388102 1101 14-15 1.991169023672338 1101 16-17 1.5716571664685937 1101 18-19 1.509799945350128 1101 20-21 1.8161406346991527 1101 22-23 1.6833397251830569 1101 24-25 1.618308366641351 1101 26-27 1.860195005316946 1101 28-29 1.8241424647018754 1101 30-31 1.863460936980438 1101 32-33 1.8862953349063467 1101 34-35 1.6559277190080621 1101 36-37 1.5787018979931986 1101 38-39 1.5160116731517945 1101 40-41 1.6758025872170492 1101 42-43 1.799535362037922 1101 44-45 1.7461795134655844 1101 46-47 1.4927909416118936 1101 48-49 1.365991697275195 1101 50-51 1.273677742140606 1101 52-53 1.1835620901795103 1101 54-55 1.1509990206017733 1101 56-57 1.2410755549681767 1101 58-59 1.0701145840647115 1101 60-61 1.0444116140958428 1101 62-63 0.8721220219782779 1101 64-65 0.8968657580266566 1101 66-67 0.9021181539540457 1101 68-69 0.6504347512449478 1101 70-71 0.6209305494029813 1101 72-73 0.5838414476731373 1101 74-75 0.5520130839384834 1101 76-77 0.8170085222876615 1101 78-79 0.7678129970178063 1101 80-81 0.9001626900843256 1101 82-83 0.9298733846073048 1101 84-85 0.9045295311101818 1101 86-87 0.8790581354620777 1101 88-89 0.8605036327670987 1101 90-91 0.9326487947487649 1101 92-93 0.9394163505640734 1101 94-95 0.7809102087941362 1101 96-97 0.7248955878014876 1101 98-99 0.8437209784483635 1101 100-101 0.8669119012267004 1101 102-103 0.7082916265760169 1101 104-105 0.8892091896144301 1101 106-107 0.8561384650488257 1101 108-109 0.7508390511107734 1101 110-111 0.8421666364794653 1101 112-113 0.8628310384728657 1101 114-115 0.8377148660895131 1101 116-117 0.8128619837544022 1101 118-119 0.8821994448142583 1101 120-121 0.8065919143474609 1101 122-123 0.9160061925210492 1101 124-125 0.8192051950598689 1102 1 0.7065378535036366 1102 2 1.075005677282224 1102 3 0.856245792503902 1102 4 0.5832196248526351 1102 5 0.6924873393037814 1102 6 0.4956215137982838 1102 7 0.5074243264013099 1102 8 0.42516380193389836 1102 9 0.980083917433241 1102 10-11 1.063642297388384 1102 12-13 1.153094767343763 1102 14-15 1.3043546457211725 1102 16-17 1.031458353265176 1102 18-19 0.8181725260741892 1102 20-21 1.1782097558788465 1102 22-23 1.1248467829945632 1102 24-25 0.7993277836278381 1102 26-27 1.1986035716518089 1102 28-29 0.7657394917894429 1102 30-31 0.7693077221377962 1102 32-33 1.103334481724655 1102 34-35 1.0200113593602325 1102 36-37 0.8727396802599898 1102 38-39 1.138017228990801 1102 40-41 1.1254580584303397 1102 42-43 1.399255438960079 1102 44-45 0.9387737350234815 1102 46-47 0.8189343702748673 1102 48-49 0.741350577053776 1102 50-51 0.7533242405720735 1102 52-53 0.6481730113788124 1102 54-55 0.6457368415228117 1102 56-57 0.6803634843667581 1102 58-59 0.6802726979811418 1102 60-61 0.5677468045546057 1102 62-63 0.4414613272978869 1102 64-65 0.20549336542971375 1102 66-67 0.21248181669670885 1102 68-69 0.12591368804116243 1102 70-71 0.3518671411148482 1102 72-73 0.019999112897984617 1102 74-75 0.21363868913146433 1102 76-77 0.03348588603880032 1102 78-79 0.13332493899570608 1102 80-81 0.2808594932040407 1102 82-83 0.22967080151275354 1102 84-85 0.16397312940250686 1102 86-87 0.23266542051687011 1102 88-89 0.2697902467455755 1102 90-91 0.36019864686111447 1102 92-93 0.34248330030326457 1102 94-95 0.34739718886462967 1102 96-97 0.047212566463127104 1102 98-99 0.3122940384346684 1102 100-101 0.3005292204278227 1102 102-103 0.18745024902357343 1102 104-105 0.30499925948392104 1102 106-107 0.22021407430460016 1102 108-109 0.38077355620731623 1102 110-111 0.3330706324380941 1102 112-113 0.17937407874571676 1102 114-115 0.27353656378140556 1102 116-117 0.30779003142950856 1102 118-119 0.29967722597244517 1102 120-121 0.3038319204615938 1102 122-123 0.08509533706125794 1102 124-125 0.04653474842382721 1103 1 0.8568064145362975 1103 2 1.2359897272596037 1103 3 0.9415654186361415 1103 4 0.5994526472712245 1103 5 0.8005831549496278 1103 6 0.9294390270578852 1103 7 0.8737670834353963 1103 8 0.9736626516456042 1103 9 1.4720959112341987 1103 10-11 1.5078400795940468 1103 12-13 1.438616776834845 1103 14-15 1.7750752363629267 1103 16-17 1.3933700939064515 1103 18-19 1.4174255606213428 1103 20-21 1.6041504414314147 1103 22-23 1.4523593687848937 1103 24-25 1.3497660001515612 1103 26-27 1.5669460976656495 1103 28-29 1.4858642427207478 1103 30-31 1.5523007819046555 1103 32-33 1.6471963219934018 1103 34-35 1.5001342332520622 1103 36-37 1.32099234902509 1103 38-39 1.3192619949384152 1103 40-41 1.4091985649422227 1103 42-43 1.6535490464374334 1103 44-45 1.3586898457976915 1103 46-47 1.192968416883204 1103 48-49 0.9979161242348482 1103 50-51 1.0345194334258494 1103 52-53 0.8028682571747261 1103 54-55 0.9278484146744113 1103 56-57 0.9409280882151698 1103 58-59 1.02662618783069 1103 60-61 1.012402776909184 1103 62-63 0.7808038340600447 1103 64-65 0.7220327303165597 1103 66-67 0.6968946883745879 1103 68-69 0.7925623062513374 1103 70-71 0.8401404573141811 1103 72-73 0.8145513667892317 1103 74-75 0.8100256287870522 1103 76-77 0.7243928330100253 1103 78-79 0.6883274898442231 1103 80-81 0.7528401067553041 1103 82-83 0.7384577527193521 1103 84-85 0.7766226909453344 1103 86-87 0.7827939255271197 1103 88-89 0.7882198866427679 1103 90-91 0.7742466028132533 1103 92-93 0.8144612667475783 1103 94-95 0.7335417497817787 1103 96-97 0.7827752451428474 1103 98-99 0.7413029615915612 1103 100-101 0.7736196937305451 1103 102-103 0.6311304014969323 1103 104-105 0.6792352081589215 1103 106-107 0.752987905486556 1103 108-109 0.8217206910750079 1103 110-111 0.7527874882525829 1103 112-113 0.7708701273369769 1103 114-115 0.8153274038287677 1103 116-117 0.7580650899065091 1103 118-119 0.9139030193825946 1103 120-121 0.8911525188040237 1103 122-123 0.7346542327844929 1103 124-125 0.6371050315198801 1104 1 1.1581210184151693 1104 2 1.5676143241164873 1104 3 0.9841000987833759 1104 4 1.1381724076535207 1104 5 0.825818308822388 1104 6 0.801296737474253 1104 7 0.7410770701992497 1104 8 0.9705549541753875 1104 9 1.3325567631115973 1104 10-11 1.4678272836191617 1104 12-13 1.3940406182579679 1104 14-15 1.3481361878739015 1104 16-17 1.1224275468497815 1104 18-19 1.1861290968881697 1104 20-21 1.425904297830641 1104 22-23 1.3419927130965554 1104 24-25 1.303135002718001 1104 26-27 1.394209240079519 1104 28-29 1.5310089962156859 1104 30-31 1.4664801565123895 1104 32-33 1.6069119675713104 1104 34-35 1.4683908166283715 1104 36-37 1.440335917066946 1104 38-39 1.5408038710737948 1104 40-41 1.476011782029488 1104 42-43 1.7099759296880173 1104 44-45 1.425712919980164 1104 46-47 1.2914055317352293 1104 48-49 1.137294714202305 1104 50-51 1.209114995692687 1104 52-53 1.0725634426568114 1104 54-55 1.1723785108487732 1104 56-57 1.0943693820844942 1104 58-59 1.1528147738832324 1104 60-61 1.0860442079041306 1104 62-63 0.9809783999497341 1104 64-65 0.9337285413566221 1104 66-67 0.8606235628466443 1104 68-69 0.8292098799356431 1104 70-71 0.8490076639906654 1104 72-73 0.8092613367748811 1104 74-75 0.7954680453465244 1104 76-77 0.6942119908267053 1104 78-79 0.6903903812063419 1104 80-81 0.8972877100604038 1104 82-83 0.9286338255409312 1104 84-85 0.933595746381787 1104 86-87 0.8832345089117197 1104 88-89 0.8050827392700093 1104 90-91 0.8408951448695987 1104 92-93 0.8444290152981253 1104 94-95 0.7843668079358856 1104 96-97 0.8050537831185629 1104 98-99 0.8453135753633632 1104 100-101 0.8008350389577679 1104 102-103 0.8016099424368051 1104 104-105 0.9073369245077352 1104 106-107 0.8772282512834053 1104 108-109 0.8194687600272417 1104 110-111 0.8656314415718747 1104 112-113 0.8603558596437324 1104 114-115 0.8887858280440746 1104 116-117 0.872441841520633 1104 118-119 0.8333386863371857 1104 120-121 0.8113097006031857 1104 122-123 0.7501265394156178 1104 124-125 0.5307496369108655 1105 1 0.990258581112812 1105 2 1.3147967084463694 1105 3 0.7307361243904751 1105 4 0.9737899921774869 1105 5 0.8978440816612654 1105 6 0.6584738733975755 1105 7 0.766634578283039 1105 8 0.77715917908262 1105 9 1.1008850015005471 1105 10-11 1.0314569821140154 1105 12-13 1.1826941467605003 1105 14-15 1.522388365001813 1105 16-17 1.0694233695425162 1105 18-19 1.2110963844227776 1105 20-21 1.0844592455647657 1105 22-23 1.2316532721019051 1105 24-25 0.8382204604683317 1105 26-27 1.294857023958862 1105 28-29 1.365638576211051 1105 30-31 1.3541194934997236 1105 32-33 1.400376835674571 1105 34-35 1.2374644058138529 1105 36-37 0.8403480172262405 1105 38-39 1.1322955875189216 1105 40-41 1.2029003776944052 1105 42-43 1.309009378171364 1105 44-45 1.1676076898289303 1105 46-47 0.6301467638737357 1105 48-49 0.7399353662937997 1105 50-51 0.9818987749537555 1105 52-53 0.6898769891104131 1105 54-55 0.7167092309213459 1105 56-57 0.738923950099803 1105 58-59 0.7651498153840741 1105 60-61 0.7284623822032721 1105 62-63 0.6231770399278602 1105 64-65 0.37831586687512697 1105 66-67 0.3873771751795587 1105 68-69 0.2683171293822788 1105 70-71 0.6146410647939824 1105 72-73 0.46587135154327797 1105 74-75 0.5384687687538978 1105 76-77 0.5574733350513164 1105 78-79 0.6154584683723279 1105 80-81 0.7670407046746845 1105 82-83 0.73601507784036 1105 84-85 0.614805440040584 1105 86-87 0.6390340782301216 1105 88-89 0.711171566449309 1105 90-91 0.4477537633656894 1105 92-93 0.5470693521249252 1105 94-95 0.4338836172489877 1105 96-97 0.5400731303625115 1105 98-99 0.5523415960895655 1105 100-101 0.5369428250549717 1105 102-103 0.41768112079815367 1105 104-105 0.5378091940252219 1105 106-107 0.5250117553133897 1105 108-109 0.4915938012118204 1105 110-111 0.5507979823995548 1105 112-113 0.5403248866387962 1105 114-115 0.6762556877382728 1105 116-117 0.5547049696430051 1105 118-119 0.4477700879419686 1105 120-121 0.363675742131651 1105 122-123 0.40087120463653747 1105 124-125 0.282011571431088 1106 1 0.3272993610406374 1106 2 -1.7130747842125906 1106 3 -0.04050125928413806 1106 4 0.342799355092513 1106 5 0.4019213166436977 1106 6 0.42662371389296183 1106 7 0.5493719715777772 1106 8 0.546308577544373 1106 9 0.9337689774393141 1106 10-11 1.0526947717529538 1106 12-13 1.1226484071296774 1106 14-15 1.2424849471119437 1106 16-17 0.8618750461333242 1106 18-19 0.8702842205704684 1106 20-21 1.1325403063549473 1106 22-23 0.8053639183513361 1106 24-25 0.9935753537383434 1106 26-27 0.9766372419621945 1106 28-29 1.0734130094442698 1106 30-31 1.1359527427451326 1106 32-33 1.1987568953370555 1106 34-35 1.0652842844152701 1106 36-37 0.7975362010762126 1106 38-39 1.168471683129276 1106 40-41 1.190675217384019 1106 42-43 1.3219603288733666 1106 44-45 1.1996445240296083 1106 46-47 0.9992929736702898 1106 48-49 0.7388599053016343 1106 50-51 0.9603185482603251 1106 52-53 0.88409957063703 1106 54-55 0.844087253036605 1106 56-57 0.9047202456680026 1106 58-59 0.7976529426625483 1106 60-61 0.8651641007314304 1106 62-63 0.8350877821029705 1106 64-65 0.8170827185155396 1106 66-67 0.6889234120797667 1106 68-69 0.6371577833676731 1106 70-71 0.7103399441799176 1106 72-73 0.5551452673811177 1106 74-75 0.5706034280536301 1106 76-77 0.54662569779072 1106 78-79 0.6526996667337279 1106 80-81 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-2.3112694677881187 2216 74-75 -1.8029295366824272 2216 76-77 -1.7295045884019693 2216 78-79 -1.692121494546484 2216 80-81 -1.5676220203553832 2216 82-83 -1.6835722072182797 2216 84-85 -1.6227938630276277 2216 86-87 -1.7257544039717025 2216 88-89 -1.808295658258107 2216 90-91 -1.65142258311867 2216 92-93 -1.5872921447981128 2216 94-95 -1.7821518482402752 2216 96-97 -1.8562127019974675 2216 98-99 -1.9197814152561783 2216 100-101 -1.8881276733347931 2216 102-103 -1.6813111538908032 2216 104-105 -1.7472767900613988 2216 106-107 -1.657837373418161 2216 108-109 -1.8221309162006136 2216 110-111 -1.7386770615287581 2216 112-113 -2.090768717157424 2216 114-115 -2.0116948626903763 2216 116-117 -1.7144993357654101 2216 118-119 -1.7883820039659781 2216 120-121 -2.0238882363120787 2216 122-123 -1.82653098297272 2216 124-125 -1.751398649807701 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 314118.0 3 38607.0 4 22736.0 5 18037.0 6 17326.0 7 19653.0 8 21360.0 9 22889.0 10 23604.0 11 24772.0 12 25280.0 13 25305.0 14 25056.0 15 21928.0 16 18636.0 17 16641.0 18 14732.0 19 13978.0 20 14043.0 21 15097.0 22 18450.0 23 25723.0 24 36266.0 25 47415.0 26 53985.0 27 59706.0 28 68049.0 29 77754.0 30 90020.0 31 103844.0 32 123962.0 33 154932.0 34 199713.0 35 268924.0 36 382176.0 37 409207.0 38 94102.0 39 96.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.46369349846473 18.655106373949504 13.86701237116507 24.0141877564207 2 13.29815789437645 19.998027929449176 42.63982870376077 24.06398547241361 3 19.15152133819665 25.771253929061764 27.901819357702877 27.175405375038707 4 12.165595038670055 17.00081617946495 37.17356674536194 33.66002203650305 5 13.689933306243777 38.31468242520507 33.93021721210094 14.065167056450203 6 31.672607001333663 37.536114404957836 17.356891107709245 13.434387485999249 7 28.63752398568697 32.66523682421632 20.19257691336127 18.50466227673544 8 25.571709136977145 36.95521017165733 19.493976884899144 17.979103806466384 9 25.71855748494101 17.787304931191642 19.750329720269907 36.743807863597446 10-11 24.32082557594494 28.076042841818023 27.24420455324711 20.358927028989932 12-13 25.106376355062686 24.865588455043802 27.69163480564549 22.33640038424802 14-15 22.04347616328265 26.296365615290153 25.482350896269878 26.177807325157314 16-17 22.479906451024213 29.799203903278677 25.518708854468024 22.202180791229083 18-19 22.359692710119848 28.3938449641977 27.46928940284451 21.77717292283794 20-21 23.0786271746557 27.418187715352882 27.57805050535117 21.925134604640245 22-23 22.766200382561376 27.387393954202487 27.181141390803436 22.665264272432704 24-25 22.754945894230268 27.42119213365343 27.30093237668456 22.522929595431737 26-27 22.617295100319552 27.554497382030462 27.357182521124862 22.47102499652512 28-29 22.399951283778492 27.6165041253129 27.519482212250146 22.464062378658465 30-31 22.511445512179186 27.26474063964893 27.6102009997828 22.613612848389078 32-33 22.402505804544713 27.363644311392203 27.485919990610764 22.747929893452323 34-35 22.85344934767585 27.26512628086391 27.348771395046928 22.53265297641331 36-37 22.849671221907982 27.272462968533794 27.14524092236753 22.732624887190696 38-39 22.594473529072634 27.36877958953665 27.48642777544166 22.55031910594906 40-41 22.766454428369105 27.242229369648662 27.4389537554817 22.55236244650053 42-43 22.614060106438806 27.069830370107965 27.589354338700588 22.72675518475264 44-45 22.742919190232875 27.35546910279182 27.19912887361141 22.702482833363895 46-47 22.63346473862888 27.53951137753226 26.867822235144217 22.95920164869464 48-49 22.82666176834783 27.409480299317384 26.921512650113634 22.842345282221153 50-51 22.74009139692805 27.418856064034635 26.965052767546737 22.875999771490577 52-53 23.032502746184466 27.46466741358094 26.679688910033782 22.82314093020081 54-55 22.74050368645141 27.293236542352666 26.933152610005546 23.033107161190372 56-57 22.75062198468921 27.627580627090566 26.87671746030117 22.74507992791905 58-59 22.927138578600516 27.283300038681308 26.825126637671076 22.9644347450471 60-61 22.970794435680165 27.157220631274193 27.121646638052404 22.750338294993234 62-63 22.84790782972605 27.41563564750876 26.92473440440303 22.811722118362162 64-65 22.743890416618427 27.21687274627592 26.920847953451382 23.11838888365427 66-67 22.69331109202955 27.657098870870833 26.6896842275165 22.959905809583116 68-69 22.699074847467898 27.37105495293532 26.92226868664658 23.007601512950203 70-71 22.6267531048992 27.333235527768746 26.967832570552524 23.07217879677953 72-73 22.900349557104587 27.210254632383812 26.840508416859155 23.048887393652446 74-75 22.74163028116826 27.358452989069903 26.767577909641794 23.132338820120047 76-77 22.840719991409458 27.24715181800088 26.853772007280075 23.058356183309588 78-79 22.830324929501625 27.399573906118786 26.801318808270917 22.968782356108672 80-81 22.601130071039876 27.6069959058867 26.933416788942814 22.858457234130608 82-83 22.80375315019989 27.544154104631787 26.751524819881077 22.900567925287245 84-85 22.766585437963837 27.298162120087955 26.862174139724427 23.073078302223784 86-87 22.805527675665545 27.247033613911075 27.04528925642452 22.902149453998867 88-89 22.848567650163613 27.21500606490163 26.948208545046175 22.98821773988858 90-91 22.733451731205477 27.45670596826173 26.989276641445127 22.820565659087663 92-93 22.710059430647828 27.350257067650247 27.02844366675058 22.91123983495135 94-95 22.694694204994565 27.212295944941587 26.97047888740548 23.122530962658363 96-97 22.75038507361294 27.324474673392928 26.875658250973927 23.049482002020206 98-99 22.662075730942846 27.173451653739058 26.84284750205826 23.32162511325984 100-101 22.449834877520054 27.268172791604385 27.12141435260942 23.16057797826614 102-103 22.746082437723548 27.093913730668767 27.06996618264111 23.09003764896658 104-105 22.695411383612367 27.000108574941073 27.276812239730052 23.027667801716508 106-107 22.49001134378932 27.263824666124272 27.17213730054749 23.074026689538922 108-109 22.570938789973024 27.189335077210696 26.88653932120805 23.353186811608232 110-111 22.38073801455964 27.275018298284348 27.194627237318958 23.149616449837055 112-113 22.521064487668 27.198238082774758 27.19456257820063 23.08613485135661 114-115 22.287569252314338 27.208381256641452 27.11360593433014 23.39044355671407 116-117 22.20755199861044 27.216357267034784 27.233778038851913 23.34231269550286 118-119 22.269222564540147 27.192310427204564 26.995756807500182 23.542710200755103 120-121 22.184586118225567 27.240899267018932 27.152398019746137 23.42211659500937 122-123 22.380396421582045 27.202852640636454 27.076903297677607 23.339847640103898 124-125 22.31916430774575 27.112848337597566 27.039781339228842 23.528206015427838 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3956.0 1 2773.5 2 1200.0 3 713.0 4 610.0 5 576.0 6 589.5 7 686.0 8 850.5 9 994.0 10 1053.0 11 1069.0 12 1126.0 13 1251.5 14 1413.0 15 1523.0 16 1595.5 17 1770.0 18 2034.0 19 2358.5 20 2805.5 21 3465.0 22 4344.0 23 5494.5 24 7156.5 25 9564.5 26 12746.5 27 16844.0 28 21277.0 29 26159.5 30 31947.5 31 36982.5 32 41638.0 33 47668.5 34 55071.0 35 61765.0 36 67981.0 37 74594.0 38 79792.5 39 83395.0 40 87102.5 41 93380.5 42 99904.0 43 105944.0 44 110734.0 45 112555.0 46 112793.0 47 114141.5 48 118787.5 49 120656.5 50 118604.0 51 114413.5 52 108761.5 53 104261.0 54 98788.0 55 90818.5 56 81707.5 57 72875.5 58 65273.5 59 57652.0 60 48693.5 61 39129.0 62 30464.0 63 23443.5 64 18321.5 65 14518.5 66 11142.0 67 8687.5 68 6501.5 69 4724.5 70 3703.0 71 2933.5 72 2380.0 73 1908.0 74 1481.5 75 1178.0 76 846.5 77 571.5 78 412.5 79 286.5 80 207.5 81 154.0 82 104.5 83 79.5 84 64.0 85 44.0 86 30.5 87 22.5 88 16.5 89 13.0 90 12.5 91 10.0 92 4.0 93 3.0 94 3.5 95 5.0 96 3.5 97 1.0 98 1.0 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21057865758325645 2 0.25046770592208933 3 0.30224150496461555 4 0.28759047607989013 5 0.32129808798950316 6 0.2339383400008606 7 0.22499062539060874 8 0.2036458863394353 9 0.20313361260220716 10-11 0.14673227413338652 12-13 0.1710994282342061 14-15 0.18814106789266294 16-17 0.16165651567796696 18-19 0.18696283829703816 20-21 0.1811058419013962 22-23 0.20410693270294067 24-25 0.19459571698173778 26-27 0.24438872424031513 28-29 0.17318267476560062 30-31 0.15323815059618418 32-33 0.1921880304167654 34-35 0.15233313366041443 36-37 0.15346013588231636 38-39 0.18527233496418521 40-41 0.2053705412547701 42-43 0.2681753014389428 44-45 0.1710994282342061 46-47 0.1569948246691907 48-49 0.15948789019036774 50-51 0.16491799180498626 52-53 0.18546016866783557 54-55 0.17258502207216775 56-57 0.1714238682677839 58-59 0.1444270423158598 60-61 0.15839503955094766 62-63 0.14739822999178312 64-65 0.14382938962242692 66-67 0.13090301565303633 68-69 0.1332423990530449 70-71 0.13500120555086162 72-73 0.11194888737559433 74-75 0.11295635905880971 76-77 0.1357866919479448 78-79 0.14065329245161232 80-81 0.15067678191004338 82-83 0.1536138180034848 84-85 0.13911647123992785 86-87 0.16624990352177949 88-89 0.16201510729402668 90-91 0.1490545817421542 92-93 0.14951562810565955 94-95 0.17227765782983087 96-97 0.1589756164531396 98-99 0.1548774265553143 100-101 0.13418156757129654 102-103 0.1730460684356731 104-105 0.13232030632603423 106-107 0.1384846669640131 108-109 0.14920826386332264 110-111 0.1252509287522856 112-113 0.11452733185297607 114-115 0.11304173801501441 116-117 0.11790833851868193 118-119 0.10129359364124856 120-121 0.09355826020910331 122-123 0.12067461669971402 124-125 0.11642274468072027 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2928122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.847247872123134 #Duplication Level Percentage of deduplicated Percentage of total 1 82.20630602523633 52.48646397444874 2 10.052423071039096 12.836390950641649 3 3.288962390456736 6.299735909567455 4 1.500492221210077 3.832091951111696 5 0.7999297272876231 2.5536655789206364 6 0.4631563904589883 1.7742756519115725 7 0.3146508564775329 1.4062713858687819 8 0.22762775228877524 1.1626724418364538 9 0.17706905384351493 1.0174834594106292 >10 0.8811930917176809 10.049188071919996 >50 0.05885332929581379 2.595350887686696 >100 0.027511740943636266 2.9843312591437123 >500 0.0014373664651845012 0.6169189021269753 >1k 3.8698327900811943E-4 0.3851595754050125 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 3.415158248187746E-5 0.0 0.0 0.0 0.0 9 3.415158248187746E-5 0.0 0.0 0.0 0.0 10-11 6.830316496375492E-5 0.0 0.0 0.0 0.0 12-13 6.830316496375492E-5 0.0 0.0 0.0 0.0 14-15 6.830316496375492E-5 0.0 0.0 0.0 0.0 16-17 6.830316496375492E-5 0.0 0.0 0.0 1.707579124093873E-5 18-19 6.830316496375492E-5 1.707579124093873E-5 0.0 0.0 3.415158248187746E-5 20-21 6.830316496375492E-5 3.415158248187746E-5 0.0 0.0 3.415158248187746E-5 22-23 6.830316496375492E-5 3.415158248187746E-5 0.0 8.537895620469365E-5 3.415158248187746E-5 24-25 6.830316496375492E-5 3.415158248187746E-5 0.0 1.3660632992750985E-4 3.415158248187746E-5 26-27 6.830316496375492E-5 3.415158248187746E-5 0.0 2.3906107737314223E-4 3.415158248187746E-5 28-29 6.830316496375492E-5 3.415158248187746E-5 0.0 3.5859161605971334E-4 5.122737372281619E-5 30-31 6.830316496375492E-5 3.415158248187746E-5 0.0 0.0012977601343113435 6.830316496375492E-5 32-33 6.830316496375492E-5 3.415158248187746E-5 0.0 0.003056566632128033 6.830316496375492E-5 34-35 6.830316496375492E-5 3.415158248187746E-5 0.0 0.00510566158104068 6.830316496375492E-5 36-37 6.830316496375492E-5 6.830316496375492E-5 0.0 0.009220927270106915 6.830316496375492E-5 38-39 6.830316496375492E-5 6.830316496375492E-5 0.0 0.017007488075974977 6.830316496375492E-5 40-41 6.830316496375492E-5 6.830316496375492E-5 0.0 0.027543251271634175 6.830316496375492E-5 42-43 6.830316496375492E-5 6.830316496375492E-5 0.0 0.03804486288481149 6.830316496375492E-5 44-45 6.830316496375492E-5 1.0245474744563238E-4 0.0 0.05146643480018934 6.830316496375492E-5 46-47 6.830316496375492E-5 1.0245474744563238E-4 0.0 0.06758598173163549 6.830316496375492E-5 48-49 6.830316496375492E-5 1.0245474744563238E-4 0.0 0.08551556253462117 6.830316496375492E-5 50-51 1.0245474744563238E-4 1.0245474744563238E-4 0.0 0.10634802784856642 6.830316496375492E-5 52-53 1.0245474744563238E-4 1.0245474744563238E-4 0.0 0.12603641514936878 6.830316496375492E-5 54-55 1.0245474744563238E-4 1.0245474744563238E-4 0.0 0.14811541322390256 6.830316496375492E-5 56-57 1.0245474744563238E-4 1.0245474744563238E-4 0.0 0.17530007287947702 6.830316496375492E-5 58-59 1.0245474744563238E-4 1.0245474744563238E-4 0.0 0.2057632844533117 6.830316496375492E-5 60-61 1.0245474744563238E-4 1.0245474744563238E-4 0.0 0.24114432390453677 6.830316496375492E-5 62-63 1.1953053868657111E-4 1.0245474744563238E-4 0.0 0.27795972982000067 6.830316496375492E-5 64-65 1.3660632992750985E-4 1.0245474744563238E-4 0.0 0.3177463234113879 6.830316496375492E-5 66-67 1.3660632992750985E-4 1.0245474744563238E-4 0.0 0.36187016797797356 6.830316496375492E-5 68-69 1.3660632992750985E-4 1.1953053868657111E-4 0.0 0.41625656308036346 6.830316496375492E-5 70-71 1.3660632992750985E-4 1.3660632992750985E-4 0.0 0.48309120999739763 6.830316496375492E-5 72-73 1.3660632992750985E-4 1.3660632992750985E-4 0.0 0.5517358907859713 6.830316496375492E-5 74-75 1.3660632992750985E-4 1.3660632992750985E-4 0.0 0.6178704302621271 6.830316496375492E-5 76-77 1.3660632992750985E-4 1.3660632992750985E-4 0.0 0.691467090510573 6.830316496375492E-5 78-79 1.3660632992750985E-4 1.3660632992750985E-4 0.0 0.7784511710919149 6.830316496375492E-5 80-81 1.3660632992750985E-4 1.3660632992750985E-4 0.0 0.8838600304222297 6.830316496375492E-5 82-83 1.3660632992750985E-4 1.3660632992750985E-4 0.0 0.9989337875949158 6.830316496375492E-5 84-85 1.3660632992750985E-4 1.3660632992750985E-4 0.0 1.1107631444318236 6.830316496375492E-5 86-87 1.3660632992750985E-4 1.3660632992750985E-4 0.0 1.2304985926132859 6.830316496375492E-5 88-89 1.3660632992750985E-4 1.3660632992750985E-4 0.0 1.3719373714619814 6.830316496375492E-5 90-91 1.3660632992750985E-4 1.3660632992750985E-4 0.0 1.534020781920972 6.830316496375492E-5 92-93 1.3660632992750985E-4 1.3660632992750985E-4 0.0 1.7144777437552126 6.830316496375492E-5 94-95 1.3660632992750985E-4 1.5368212116844858E-4 0.0 1.8916903052536744 6.830316496375492E-5 96-97 1.3660632992750985E-4 1.707579124093873E-4 0.0 2.070183551095207 6.830316496375492E-5 98-99 1.3660632992750985E-4 1.707579124093873E-4 0.0 2.2743417111718705 6.830316496375492E-5 100-101 1.5368212116844858E-4 1.707579124093873E-4 0.0 2.518593829082258 6.830316496375492E-5 102-103 1.707579124093873E-4 1.707579124093873E-4 0.0 2.789945227692016 6.830316496375492E-5 104-105 1.707579124093873E-4 1.8783370365032604E-4 0.0 3.05905969764921 6.830316496375492E-5 106-107 1.707579124093873E-4 2.0490949489126477E-4 0.0 3.3304110962589673 6.830316496375492E-5 108-109 1.707579124093873E-4 2.0490949489126477E-4 0.0 3.616703812204546 6.830316496375492E-5 110-111 1.707579124093873E-4 2.0490949489126477E-4 0.0 3.9488108760495635 6.830316496375492E-5 112-113 1.707579124093873E-4 2.0490949489126477E-4 0.0 4.312337395777908 6.830316496375492E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5840 0.0 48.559277 1 ATCAACG 6720 0.0 40.42651 3 GGTATCA 3130 0.0 39.781403 1 TCAACGC 6760 0.0 39.748013 4 CAACGCA 6780 0.0 39.28129 5 AACGCAG 6820 0.0 37.183163 6 TATCAAC 7480 0.0 37.115475 2 GTACATG 11200 0.0 35.107834 1 TACATGG 11220 0.0 32.97383 2 ACATGGG 11610 0.0 30.788582 3 ACGCAGA 8525 0.0 29.184414 7 CGCAGAG 8490 0.0 27.405268 8 CGCATAG 385 0.0 23.184381 8 CATGGGA 7225 0.0 23.006573 4 CATGGGG 5540 0.0 22.69124 4 GCAGAGT 9650 0.0 22.567791 9 GTACCTG 2895 0.0 22.019798 1 GAGTACA 8835 0.0 21.511082 1 TACCTGG 2990 0.0 20.722055 2 GTGTAGC 1565 0.0 20.176214 1 >>END_MODULE