FastQCFastQC Report
Thu 26 May 2016
SRR1364631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2928122
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64940.22178037663731226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51210.1748902538896945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33840.11556895511867334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.052.0825461
GTATCAA93650.047.268991
ATCAACG112300.038.889523
TCAACGC113450.038.28884
TATCAAC114800.038.0725362
CAACGCA114150.037.2027665
AACGCAG118600.036.048116
AACGCCG2750.034.644166
ACGCCGA3450.029.3413477
ACGCAGA144800.029.3204887
CGCAGAG146400.028.9593728
GTACATG114900.028.6229421
TACATGG115100.027.6263542
ACATGGG118350.026.1930313
GCAGAGT159850.025.070339
GAGTACT88850.022.75423412-13
CATGGGG59200.022.5154024
AGTACTT91350.022.22929812-13
GTACTTT94550.021.8548514-15
AGAGTAC153000.021.51345310-11
Produced by FastQC (version 0.11.5)