##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364631_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2928122 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.772629009310403 31.0 30.0 33.0 26.0 34.0 2 29.820857874091313 31.0 30.0 34.0 26.0 34.0 3 29.847671989076957 31.0 30.0 34.0 26.0 34.0 4 33.55062391526036 35.0 33.0 37.0 28.0 37.0 5 33.00552231088732 35.0 33.0 37.0 26.0 37.0 6 33.05625926788569 35.0 33.0 37.0 26.0 37.0 7 32.86780332240255 35.0 33.0 37.0 26.0 37.0 8 32.81450260610726 35.0 33.0 37.0 25.0 37.0 9 34.128849480998404 38.0 34.0 39.0 25.0 39.0 10-11 33.944376805338024 38.0 33.5 39.0 25.0 39.0 12-13 33.81020206808323 37.5 33.0 39.0 24.0 39.0 14-15 34.83640213761585 39.0 34.0 41.0 24.5 41.0 16-17 34.76307203046868 39.0 33.5 41.0 24.0 41.0 18-19 34.781109188756474 39.0 33.5 41.0 24.0 41.0 20-21 34.79704961063781 39.0 34.0 41.0 24.0 41.0 22-23 34.791716499517435 39.0 34.0 41.0 24.0 41.0 24-25 34.787076665521454 39.0 34.0 41.0 24.0 41.0 26-27 34.71474941959386 39.0 34.0 41.0 23.5 41.0 28-29 34.48274149779279 39.0 33.0 41.0 22.0 41.0 30-31 34.430801209785656 38.5 33.0 41.0 22.5 41.0 32-33 34.28235879515949 38.0 33.0 41.0 21.5 41.0 34-35 34.16819961736567 38.0 33.0 41.0 20.5 41.0 36-37 34.01828663559783 38.0 33.0 41.0 19.0 41.0 38-39 33.90262342211151 38.0 33.0 41.0 18.0 41.0 40-41 33.74210517867766 38.0 33.0 40.0 16.0 41.0 42-43 33.58807214999921 38.0 33.0 40.0 15.0 41.0 44-45 33.409951839438385 38.0 32.5 40.0 14.0 41.0 46-47 33.19389595105669 38.0 32.0 40.0 9.0 41.0 48-49 32.97471280226712 38.0 32.0 40.0 8.5 41.0 50-51 32.78794940921178 38.0 32.0 40.0 7.0 41.0 52-53 32.627551550106176 38.0 31.0 40.0 4.0 41.0 54-55 32.41653660605671 38.0 31.0 40.0 2.0 41.0 56-57 32.194638918733574 37.0 31.0 40.0 2.0 41.0 58-59 32.010102550371876 37.0 31.0 40.0 2.0 41.0 60-61 31.803076169640473 36.5 30.5 40.0 2.0 41.0 62-63 31.536633036465012 36.0 30.0 40.0 2.0 41.0 64-65 31.26300594715657 36.0 30.0 39.0 2.0 41.0 66-67 30.989009337725683 35.0 29.5 39.0 2.0 41.0 68-69 30.675156123959315 35.0 29.0 39.0 2.0 40.5 70-71 30.409946204427275 35.0 29.0 38.0 2.0 40.0 72-73 30.251243288360254 35.0 29.0 37.5 2.0 40.0 74-75 29.956222281721868 35.0 29.0 37.0 2.0 39.0 76-77 29.65947474183111 35.0 29.0 36.5 2.0 39.0 78-79 29.344745198458263 34.0 29.0 36.0 2.0 39.0 80-81 29.062420213365428 34.0 29.0 36.0 2.0 37.5 82-83 28.80377064207024 34.0 29.0 35.0 2.0 37.0 84-85 28.787495363922677 34.0 29.0 35.0 2.0 37.0 86-87 28.63766468746862 34.0 29.0 35.0 2.0 36.0 88-89 28.504117485541926 34.0 29.0 35.0 2.0 36.0 90-91 28.35430337260538 34.0 29.0 35.0 2.0 36.0 92-93 28.22282763491412 34.0 29.0 35.0 2.0 35.5 94-95 28.137549425877744 34.0 29.0 35.0 2.0 35.0 96-97 28.059307467380115 34.0 29.0 35.0 2.0 35.0 98-99 27.973876259254226 34.0 29.0 35.0 2.0 35.0 100-101 27.880839493709622 34.0 28.0 35.0 2.0 35.0 102-103 26.689562285997646 32.5 25.0 34.5 2.0 35.0 104-105 27.633168631634884 34.0 27.0 35.0 2.0 35.0 106-107 27.806856749821215 34.0 28.0 35.0 2.0 35.0 108-109 27.8197981504869 34.0 29.0 35.0 2.0 35.0 110-111 27.77072352176583 34.0 28.0 35.0 2.0 35.0 112-113 27.727167447258005 34.0 28.5 35.0 2.0 35.0 114-115 27.652142567830165 34.0 28.0 35.0 2.0 35.0 116-117 27.5409480889116 34.0 27.5 35.0 2.0 35.0 118-119 27.44156459327856 34.0 27.0 35.0 2.0 35.0 120-121 27.310393316945127 34.0 27.0 35.0 2.0 35.0 122-123 27.13020444503337 34.0 27.0 35.0 2.0 35.0 124-125 26.318184146698805 33.0 25.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7279012441784865 1101 2 0.6737303811822244 1101 3 0.6407062182388223 1101 4 0.7069900507528573 1101 5 0.9451419043772162 1101 6 0.9214543774880042 1101 7 0.8410346346775697 1101 8 0.9822746029005316 1101 9 1.069749092144228 1101 10-11 1.212207927081586 1101 12-13 1.1948068666559593 1101 14-15 1.2091860530586658 1101 16-17 1.344223649567354 1101 18-19 1.298413444072267 1101 20-21 1.3589647120086852 1101 22-23 1.2566722419037362 1101 24-25 1.1081389867318165 1101 26-27 1.3888520830457551 1101 28-29 1.3923254459941745 1101 30-31 1.3546734763310297 1101 32-33 1.394135030046435 1101 34-35 1.2542350604132437 1101 36-37 1.3559657990862632 1101 38-39 1.4730678755298925 1101 40-41 1.4771056501029847 1101 42-43 1.414160056856126 1101 44-45 1.326406964627303 1101 46-47 1.510857925680213 1101 48-49 1.586924450711848 1101 50-51 1.5393665710025815 1101 52-53 1.435576974820286 1101 54-55 1.4137605300780223 1101 56-57 1.4747133763325024 1101 58-59 1.5167850303865649 1101 60-61 1.431238325217361 1101 62-63 1.5507285066693761 1101 64-65 1.5762159243364344 1101 66-67 1.4622438446069914 1101 68-69 1.5580414597786039 1101 70-71 1.5111934616382712 1101 72-73 1.5677055315616286 1101 74-75 1.4095547721769073 1101 76-77 1.463965971362672 1101 78-79 1.5101883303825083 1101 80-81 1.4990569567536483 1101 82-83 1.4628041312864077 1101 84-85 1.4560636518057493 1101 86-87 1.4396565721192545 1101 88-89 1.3751563997556495 1101 90-91 1.4406626476155857 1101 92-93 1.450068954161786 1101 94-95 1.368789040458367 1101 96-97 1.4196547951194844 1101 98-99 1.4548850271353722 1101 100-101 1.4227905510638799 1101 102-103 1.459883299071933 1101 104-105 1.4362195721501685 1101 106-107 1.4160602602539356 1101 108-109 1.3581592471651334 1101 110-111 1.4261190759919025 1101 112-113 1.411389027884713 1101 114-115 1.413745961734751 1101 116-117 1.3816843377191326 1101 118-119 1.4889057300106465 1101 120-121 1.498540851139012 1101 122-123 1.5779290948599112 1101 124-125 1.4722852064096301 1102 1 0.47750404609679364 1102 2 -0.45562102957172 1102 3 0.08118844516002355 1102 4 0.3026534791554738 1102 5 0.6984805308854547 1102 6 0.6600079129866074 1102 7 0.6623836300136503 1102 8 0.5599522071856313 1102 9 0.8098178803221217 1102 10-11 0.9441939941266426 1102 12-13 1.0336172765106326 1102 14-15 1.1144493501922241 1102 16-17 1.1165141151126932 1102 18-19 1.1208898898638395 1102 20-21 1.06939731095337 1102 22-23 1.0962491752930887 1102 24-25 1.0711447122211695 1102 26-27 1.050838011515033 1102 28-29 1.06692688840009 1102 30-31 1.044129455109612 1102 32-33 1.0480925128971634 1102 34-35 0.9967058550424568 1102 36-37 0.9097646441621166 1102 38-39 0.8064893147149661 1102 40-41 0.7938680495384389 1102 42-43 0.7664438382196508 1102 44-45 0.6878102396769208 1102 46-47 0.7153432877734858 1102 48-49 0.7625943701300315 1102 50-51 0.6856742508093845 1102 52-53 0.7611104600830672 1102 54-55 0.7110244247671531 1102 56-57 0.4501091939054689 1102 58-59 0.731767612880617 1102 60-61 0.5431524409873454 1102 62-63 0.7496000273937007 1102 64-65 0.7481669737015331 1102 66-67 0.598704796576417 1102 68-69 0.42885628747117366 1102 70-71 0.29350201132303866 1102 72-73 0.3064086134240114 1102 74-75 0.4753352782178659 1102 76-77 0.5092746352674808 1102 78-79 0.4536061854939675 1102 80-81 0.40333465988947026 1102 82-83 0.3205820486897828 1102 84-85 0.34056333654584847 1102 86-87 0.35128304341086647 1102 88-89 0.4211042969416603 1102 90-91 0.39824286882032567 1102 92-93 0.5868280015330747 1102 94-95 0.3932109747369168 1102 96-97 0.34153747518348965 1102 98-99 0.4178016756927079 1102 100-101 0.19506967859075885 1102 102-103 0.17633969273801142 1102 104-105 0.1606994772971433 1102 106-107 0.3816951462449083 1102 108-109 0.4327607578091204 1102 110-111 0.39303946231598275 1102 112-113 0.412441390213349 1102 114-115 0.5201112985148413 1102 116-117 0.5787206216877827 1102 118-119 0.49299893839062037 1102 120-121 0.4856049069539772 1102 122-123 0.48999299845147704 1102 124-125 0.3902591308568546 1103 1 0.4135595679618973 1103 2 -0.879379055118708 1103 3 0.1629869377529367 1103 4 0.4795076572528316 1103 5 0.7415056447110544 1103 6 0.6946460294015537 1103 7 0.6731515798052499 1103 8 0.6927647823875347 1103 9 0.8867784239897603 1103 10-11 1.0110630441521522 1103 12-13 1.0537398616271503 1103 14-15 1.164479671599139 1103 16-17 1.1786024899815288 1103 18-19 1.2416085553368958 1103 20-21 1.1881939361326488 1103 22-23 1.146800495477514 1103 24-25 0.9335606064138986 1103 26-27 1.028837905630759 1103 28-29 1.1176276498043052 1103 30-31 1.031870943457534 1103 32-33 1.0114604030085346 1103 34-35 1.1012783713342031 1103 36-37 1.1983334737558096 1103 38-39 1.1945198190129034 1103 40-41 1.2572022491976824 1103 42-43 1.2065662789485216 1103 44-45 1.2102004328675946 1103 46-47 1.1563041394681193 1103 48-49 1.1772634336317154 1103 50-51 1.127296397950019 1103 52-53 1.0767185246147264 1103 54-55 1.1176927278272046 1103 56-57 1.0113444783972767 1103 58-59 1.1011535803260202 1103 60-61 1.0636785895911167 1103 62-63 1.1528559689455768 1103 64-65 1.103133668283153 1103 66-67 1.0929497454150585 1103 68-69 1.0337943451684772 1103 70-71 1.104876629898996 1103 72-73 1.0271494183370393 1103 74-75 1.0393004519162083 1103 76-77 1.0038557666891208 1103 78-79 1.012075724512325 1103 80-81 1.0595955491248894 1103 82-83 1.00684813588067 1103 84-85 1.105715617389457 1103 86-87 1.0348461937569162 1103 88-89 0.9824002242472893 1103 90-91 1.0171908603880873 1103 92-93 0.9804891429655918 1103 94-95 0.8464693227496589 1103 96-97 0.9427748773608542 1103 98-99 0.9452146340031788 1103 100-101 0.9911478693172207 1103 102-103 1.0503140591168645 1103 104-105 1.044095378613001 1103 106-107 1.0183532459108946 1103 108-109 0.9969081005950322 1103 110-111 1.0599092342797505 1103 112-113 1.0007539317248018 1103 114-115 0.9460762893221641 1103 116-117 0.9547705605636772 1103 118-119 1.0249276205163866 1103 120-121 1.0193762161649431 1103 122-123 1.0633226739642936 1103 124-125 1.032238156143947 1104 1 0.42737492271748323 1104 2 -0.5587719945990806 1104 3 0.14277468303069796 1104 4 0.41718473469565964 1104 5 0.7353575179672092 1104 6 0.6949613458365391 1104 7 0.8377801599336365 1104 8 0.8112966268100195 1104 9 0.9051608698386033 1104 10-11 1.012065024395291 1104 12-13 0.8976750304138861 1104 14-15 0.9416685290029889 1104 16-17 1.1680489695608003 1104 18-19 1.072011372212053 1104 20-21 1.0447899996744283 1104 22-23 1.147756698133442 1104 24-25 1.0899488583522015 1104 26-27 1.032716416885627 1104 28-29 1.2891609296959246 1104 30-31 1.0807139201192513 1104 32-33 1.0866130899043398 1104 34-35 1.0507301018719986 1104 36-37 1.084062000235349 1104 38-39 1.1165250378649816 1104 40-41 1.1998369771938826 1104 42-43 1.1101410217344139 1104 44-45 1.0906707509700482 1104 46-47 1.171073470141792 1104 48-49 1.261326774484175 1104 50-51 1.1557638689351108 1104 52-53 1.0144301423863666 1104 54-55 1.1455733498224063 1104 56-57 1.2071858642360453 1104 58-59 1.2025213152054306 1104 60-61 1.2215997692857563 1104 62-63 1.265345249533187 1104 64-65 1.2391076537710326 1104 66-67 1.2226794919397896 1104 68-69 1.2548758303094054 1104 70-71 1.2872371636118025 1104 72-73 1.1883759573235935 1104 74-75 1.1847921622372972 1104 76-77 1.246768577992249 1104 78-79 1.243827970410468 1104 80-81 1.136094324936927 1104 82-83 1.2269365736221154 1104 84-85 1.2178392469099961 1104 86-87 1.1629261605733419 1104 88-89 1.1543492826996733 1104 90-91 1.1550174140814669 1104 92-93 1.2071727639105987 1104 94-95 1.0903265298197056 1104 96-97 1.0968331588578017 1104 98-99 1.1820953678531119 1104 100-101 1.2105977252524625 1104 102-103 1.223945443899236 1104 104-105 1.2083554595212576 1104 106-107 1.2555656860220026 1104 108-109 1.1684545078829913 1104 110-111 0.9939595771973799 1104 112-113 1.016246097750372 1104 114-115 1.176227225898451 1104 116-117 1.1879916507745243 1104 118-119 1.0432735841138872 1104 120-121 1.029765398154673 1104 122-123 1.103854998947881 1104 124-125 1.0324448025939006 1105 1 0.5426204328477908 1105 2 0.3977231787484605 1105 3 0.49526953771068705 1105 4 0.5365316883979929 1105 5 0.7255947711649924 1105 6 0.5887148913133231 1105 7 0.7118496787015971 1105 8 0.7295429864535805 1105 9 0.7941687308386989 1105 10-11 0.9645036418585136 1105 12-13 0.9480750712882013 1105 14-15 0.9774896293988817 1105 16-17 1.0867641478244678 1105 18-19 1.1064063752566113 1105 20-21 1.1062635628780981 1105 22-23 1.0444639235198778 1105 24-25 1.0657447365715456 1105 26-27 1.0973984739407285 1105 28-29 1.2146198450012236 1105 30-31 1.1690827646010717 1105 32-33 1.1058318509761378 1105 34-35 1.0978203138139406 1105 36-37 1.1454968309191855 1105 38-39 1.1320536006462945 1105 40-41 1.0533014960038827 1105 42-43 1.001257252922123 1105 44-45 1.0224277041078977 1105 46-47 1.2440634024889903 1105 48-49 1.262537240791687 1105 50-51 1.2109029386980836 1105 52-53 1.1934650601032857 1105 54-55 1.097849681030084 1105 56-57 1.0893760511169504 1105 58-59 1.066343580379268 1105 60-61 1.2276871802760176 1105 62-63 1.1523444991031262 1105 64-65 1.0769348434795099 1105 66-67 1.0370798624909092 1105 68-69 1.034211985945678 1105 70-71 1.0463725086474973 1105 72-73 0.927595055678573 1105 74-75 1.0615745337163887 1105 76-77 1.130782045699739 1105 78-79 1.0880629901330252 1105 80-81 0.8993588868631193 1105 82-83 0.8729548205422297 1105 84-85 0.7993112257834554 1105 86-87 0.8145001470924242 1105 88-89 0.8573689880442821 1105 90-91 0.8551558753335335 1105 92-93 0.6753347827416079 1105 94-95 0.7667808808928669 1105 96-97 0.7859368052793378 1105 98-99 0.8747481691450822 1105 100-101 0.9009635814253052 1105 102-103 0.5749388379528817 1105 104-105 0.5305584311362281 1105 106-107 0.8580230842535563 1105 108-109 0.9063276511740952 1105 110-111 0.8772857178250497 1105 112-113 0.6831821822869486 1105 114-115 0.821599405380347 1105 116-117 0.7220097874020368 1105 118-119 0.58930290533198 1105 120-121 0.5361271895147013 1105 122-123 0.6118470090395505 1105 124-125 0.48928163780074385 1106 1 0.4978644166657098 1106 2 0.38460049283734676 1106 3 0.49885009380110645 1106 4 0.39573376528192483 1106 5 0.5095908014736494 1106 6 0.6618437291831327 1106 7 0.6070858867035369 1106 8 0.5710638163843953 1106 9 0.68611013242338 1106 10-11 0.8594191380480325 1106 12-13 0.6178775457809706 1106 14-15 0.7781131709105509 1106 16-17 0.9244601541640023 1106 18-19 0.862112080748048 1106 20-21 0.8945838759105413 1106 22-23 0.8656414139252249 1106 24-25 0.8586304259257815 1106 26-27 0.9496027593197667 1106 28-29 1.1181368885889498 1106 30-31 0.9794050397451883 1106 32-33 0.8552984507661208 1106 34-35 0.7734510089089994 1106 36-37 0.898182186477257 1106 38-39 1.0125884136121428 1106 40-41 1.0664308495713897 1106 42-43 0.9913605071173421 1106 44-45 1.0383878345735127 1106 46-47 0.926068401705848 1106 48-49 0.9725327909306642 1106 50-51 1.0061469608980858 1106 52-53 0.9523196954998454 1106 54-55 0.9601759116936961 1106 56-57 0.8145489833057056 1106 58-59 0.8499241738676133 1106 60-61 0.9500872531429216 1106 62-63 0.9433553468142044 1106 64-65 0.8255185077803837 1106 66-67 0.8212975493180217 1106 68-69 0.8687544368472437 1106 70-71 0.9699398347298 1106 72-73 1.0385348935871086 1106 74-75 0.9913380563017604 1106 76-77 1.0460839874442982 1106 78-79 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116-117 -2.262455769368355 2216 118-119 -2.418252288391976 2216 120-121 -2.4695745810820107 2216 122-123 -2.4655848459741954 2216 124-125 -2.560604647363121 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 100671.0 3 31335.0 4 14716.0 5 11470.0 6 12043.0 7 14361.0 8 16310.0 9 17852.0 10 18954.0 11 21320.0 12 23067.0 13 25421.0 14 27339.0 15 23238.0 16 16143.0 17 14051.0 18 13107.0 19 12256.0 20 11825.0 21 12683.0 22 15035.0 23 19882.0 24 27325.0 25 36605.0 26 44494.0 27 50355.0 28 58833.0 29 68944.0 30 81261.0 31 95614.0 32 116681.0 33 144358.0 34 186924.0 35 253944.0 36 405847.0 37 636366.0 38 246540.0 39 952.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.4672768303201 19.826901601542904 13.583453958204336 25.122367609932656 2 15.303840929954687 20.99681261120487 38.61272371702987 25.086622741810572 3 19.517526933645524 24.68531024322074 28.83356636096447 26.96359646216927 4 13.242588671751857 17.203721913254405 36.08287176012151 33.470817654872235 5 13.611844276380115 38.2798018301536 33.76932658191178 14.339027311554512 6 32.43640879104216 37.10118140771014 16.747213311489944 13.715196489757753 7 28.454825311240445 32.34352940212191 20.400140431307165 18.80150485533048 8 25.97791348857732 36.75946562335859 18.856864570533606 18.405756317530486 9 25.970926589039667 16.5244074239141 19.549094038635907 37.955571948410324 10-11 25.040843433561633 27.24538078791861 26.846429031522828 20.867346746996926 12-13 25.76053377142574 24.049087297995626 27.817780654372072 22.372598276206563 14-15 22.51276722369132 25.510112597477892 25.730218594678966 26.24690158415182 16-17 22.37037388857064 28.93163198483691 26.118194937743894 22.579799188848558 18-19 22.275104149715354 27.36545830741366 28.4987977766595 21.860639766211488 20-21 22.904216798439663 26.600714420992645 28.58293638060055 21.912132399967142 22-23 22.51257881076229 26.47051088193935 28.483086447879096 22.533823859419257 24-25 22.46627701987827 26.58014249406275 28.625975966848376 22.327604519210606 26-27 22.336996204393124 26.965730936074383 28.435512591346946 22.261760268185547 28-29 22.15591716668811 26.818934236353176 28.61720046422081 22.407948132737904 30-31 22.35656506115524 26.584855412445247 28.713011274803442 22.345568251596074 32-33 22.216277737411065 26.681261297106317 28.576997917532935 22.52546304794968 34-35 22.573700822575017 26.632138278391405 28.456959785145564 22.337201113888014 36-37 22.543841285139028 26.537104537076534 28.402243897874367 22.51681027991007 38-39 22.4016793016138 26.7636901741116 28.50342301311216 22.331207511162447 40-41 22.535860869185097 26.55565580942324 28.461416566659448 22.447066754732216 42-43 22.52515136068797 26.52380574261298 28.346076575572766 22.604966321126287 44-45 22.530413691779234 26.8179570386753 28.043845167653537 22.60778410189193 46-47 22.542095703402815 26.98378601009966 27.67658560383208 22.797532682665445 48-49 22.67752779173251 27.020947577608695 27.559279523817164 22.742245106841626 50-51 22.677456315752625 27.078700802393346 27.42378629254657 22.820056589307455 52-53 22.898538548835308 27.157521369535438 27.192253588227523 22.75168649340173 54-55 22.664988002549073 26.966243209811612 27.42349874766147 22.945270039977842 56-57 22.66023058819833 27.3393083583526 27.362156118162197 22.63830493528688 58-59 22.749636584410858 27.011191304732403 27.39423551925697 22.84493659159977 60-61 22.837590100412484 26.896198314141284 27.64193226921556 22.624279316230673 62-63 22.743024368520164 27.00853994471542 27.53913600594511 22.70929968081931 64-65 22.59439896943551 26.81151352547587 27.611775401420168 22.98231210366845 66-67 22.613658174078815 27.258973499055028 27.359396227342987 22.767972099523178 68-69 22.507803695228986 26.985314709197088 27.569140398210447 22.93774119736348 70-71 22.393756134478004 27.0081984288906 27.49239956531866 23.10564587131274 72-73 22.721117592415506 26.945438649217234 27.39889278981449 22.934550968552774 74-75 22.569797238199097 27.11486989317343 27.26988948493127 23.045443383696202 76-77 22.631231048986063 27.05934564924763 27.32368011636181 22.985743185404502 78-79 22.666191501638174 27.09461800471329 27.307229879524492 22.931960614124048 80-81 22.464247360164315 27.367612904129185 27.30619020272907 22.861949532977434 82-83 22.640646299421487 27.25255296365428 27.204484521022305 22.90231621590193 84-85 22.62488083230153 27.02599601826632 27.28746057839471 23.061662571037438 86-87 22.62484305130295 27.01911627872825 27.471353927871135 22.884686742097664 88-89 22.660309235749057 26.976864351963474 27.398038742921234 22.964787669366235 90-91 22.55870827786547 27.173645770224052 27.449573480886386 22.818072471024088 92-93 22.585519787249268 27.02008088639991 27.432402409412642 22.961996916938173 94-95 22.48126298151905 26.9469311488321 27.41044491549664 23.161360954152215 96-97 22.560008797914865 27.03903886878487 27.285028705075693 23.11592362822457 98-99 22.488501532132958 26.872510657480987 27.232521346979233 23.406466463406826 100-101 22.221216644243228 26.986984614486165 27.539405517856817 23.252393223413794 102-103 22.615563543620247 26.786569600922917 27.375155752747027 23.222711102709813 104-105 22.605294438170965 26.61040285194155 27.577289176757503 23.207013533129984 106-107 22.38537154722903 26.883829108168822 27.550856089966075 23.17994325463607 108-109 22.453397868753274 26.766266923428567 27.28375246691048 23.496582740907673 110-111 22.26990944323092 26.825376659604082 27.537918683327256 23.36679521383774 112-113 22.40884012996334 26.773561234217986 27.479046254742695 23.33855238107598 114-115 22.13415247419964 26.80020224660391 27.36515740451558 23.700487874680874 116-117 22.08311454568585 26.758638590128133 27.554521881753068 23.603724982432954 118-119 22.156989476829814 26.75899165403744 27.311917009968194 23.77210185916455 120-121 22.002018601187935 26.82543369185421 27.432807221878768 23.73974048507909 122-123 22.200909631903883 26.793432276649682 27.287710577565882 23.717947513880553 124-125 22.0469282534834 26.763830932060774 27.227874513053074 23.961366301402755 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 119.0 1 95.0 2 139.0 3 266.0 4 231.5 5 213.5 6 563.0 7 1150.5 8 1787.0 9 2121.5 10 1915.5 11 1613.5 12 1616.0 13 1872.5 14 2118.0 15 2363.5 16 2612.5 17 2863.5 18 3094.5 19 3390.0 20 3783.5 21 4300.0 22 5197.0 23 6291.5 24 7727.5 25 9876.0 26 12890.5 27 16715.5 28 20596.0 29 25229.5 30 31138.0 31 36133.0 32 40406.5 33 46090.5 34 53252.0 35 59703.5 36 66203.0 37 73498.5 38 78846.0 39 82135.5 40 85720.5 41 92159.0 42 99307.5 43 106092.5 44 110966.0 45 112847.0 46 112882.5 47 113438.5 48 119270.5 49 122731.5 50 120643.0 51 116538.5 52 110392.5 53 105676.5 54 101103.0 55 93104.5 56 83042.0 57 73858.5 58 66232.0 59 58794.0 60 49598.0 61 39094.0 62 30060.5 63 22881.5 64 17635.5 65 13652.0 66 10206.5 67 7975.5 68 5815.5 69 3877.5 70 2837.0 71 2247.0 72 1930.0 73 1599.0 74 1240.5 75 987.0 76 641.5 77 378.0 78 232.0 79 133.0 80 79.5 81 40.0 82 26.5 83 16.0 84 8.5 85 5.5 86 7.0 87 8.5 88 3.5 89 1.5 90 1.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057852780724300425 2 0.07567990677984045 3 0.0 4 0.009323382017552548 5 0.03196588120303731 6 1.707579124093873E-4 7 0.0 8 0.0 9 5.805769021919168E-4 10-11 0.05816014496663732 12-13 0.009459988347480056 14-15 0.007325514442362715 16-17 0.05264466439581411 18-19 1.8783370365032604E-4 20-21 0.010125944205876668 22-23 0.013011752925595312 24-25 0.0 26-27 0.0 28-29 0.0511419947666115 30-31 0.0 32-33 0.003568840369356195 34-35 0.0 36-37 1.0245474744563238E-4 38-39 0.0 40-41 0.0 42-43 0.0033980824569468074 44-45 0.0 46-47 8.537895620469365E-5 48-49 1.0245474744563238E-4 50-51 2.3906107737314223E-4 52-53 1.707579124093873E-4 54-55 0.0 56-57 0.0015368212116844858 58-59 1.7075791240938734E-5 60-61 0.0 62-63 0.0 64-65 6.147284846737943E-4 66-67 0.0 68-69 7.513348146013041E-4 70-71 0.0 72-73 0.0014343664642388534 74-75 0.003585916160597134 76-77 4.610463635053458E-4 78-79 5.635011109509781E-4 80-81 0.0017588064978166893 82-83 1.8783370365032604E-4 84-85 1.7075791240938734E-5 86-87 5.293495284691007E-4 88-89 0.0 90-91 0.0 92-93 0.006437573297833901 94-95 0.0055154805708232104 96-97 0.005310571075931946 98-99 4.951979459872233E-4 100-101 3.5859161605971334E-4 102-103 1.1953053868657111E-4 104-105 3.9274319854159086E-4 106-107 0.004422629931403132 108-109 0.004012810941620602 110-111 4.7812215474628446E-4 112-113 0.0022369286525629737 114-115 4.610463635053458E-4 116-117 0.0018612612452623216 118-119 0.002032019157671709 120-121 0.012021357033620866 122-123 4.951979459872233E-4 124-125 0.0019295644102260766 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2928122.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.669468872226105 #Duplication Level Percentage of deduplicated Percentage of total 1 76.9343921447014 42.0596235655839 2 12.420116348067102 13.580023281601619 3 4.3388443001004235 7.116069402173272 4 2.0425270676826672 4.466554797894274 5 1.1590237533432393 3.168160650278444 6 0.6830544981993821 2.2405335976407086 7 0.4538289785822894 1.7367412452543043 8 0.32413374270428463 1.4176175645768028 9 0.22421911490847157 1.1032145930742099 >10 1.2704818729381104 12.658030405568145 >50 0.09495994019006837 3.584486333652205 >100 0.05115256636631155 4.978696691575718 >500 0.002512055551032977 0.9522309050365139 >1k 6.280138876944364E-4 0.5392370143629038 >5k 1.2560277753888727E-4 0.3987799517270552 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6494 0.22178037663731226 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5121 0.1748902538896945 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3384 0.11556895511867334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 5.122737372281619E-5 0.0 24-25 0.0 0.0 0.0 1.0245474744563238E-4 0.0 26-27 0.0 0.0 0.0 1.707579124093873E-4 0.0 28-29 0.0 0.0 0.0 3.244400335778359E-4 0.0 30-31 0.0 0.0 0.0 0.0013660632992750985 0.0 32-33 0.0 0.0 0.0 0.0031931729620555426 0.0 34-35 0.0 0.0 0.0 0.0053788742408957 0.0 36-37 0.0 0.0 0.0 0.009903958919744464 0.0 38-39 0.0 0.0 0.0 0.018424778748972893 0.0 40-41 0.0 0.0 0.0 0.029814331506679025 0.0 42-43 0.0 0.0 0.0 0.04106727793445765 0.0 44-45 0.0 0.0 0.0 0.05510357833450928 0.0 46-47 1.707579124093873E-5 0.0 0.0 0.07176955058566548 0.0 48-49 3.415158248187746E-5 0.0 0.0 0.08973328297113303 0.0 50-51 3.415158248187746E-5 0.0 0.0 0.11071943040624674 0.0 52-53 3.415158248187746E-5 0.0 0.0 0.13066395457566315 0.0 54-55 3.415158248187746E-5 0.0 0.0 0.1529478621450882 0.0 56-57 3.415158248187746E-5 0.0 0.0 0.18093508398898678 0.0 58-59 3.415158248187746E-5 0.0 0.0 0.21252529778472345 0.0 60-61 3.415158248187746E-5 0.0 0.0 0.24906749104033232 0.0 62-63 5.122737372281619E-5 0.0 0.0 0.28690744443025257 0.0 64-65 6.830316496375492E-5 0.0 0.0 0.3271550843851452 0.0 66-67 6.830316496375492E-5 0.0 0.0 0.3715692174028268 0.0 68-69 6.830316496375492E-5 0.0 0.0 0.4275095095081421 0.0 70-71 6.830316496375492E-5 0.0 0.0 0.4963078724178842 0.0 72-73 6.830316496375492E-5 0.0 0.0 0.5661820101758055 0.0 74-75 6.830316496375492E-5 0.0 0.0 0.6329995813015987 3.415158248187746E-5 76-77 6.830316496375492E-5 0.0 0.0 0.7080135322230425 3.415158248187746E-5 78-79 6.830316496375492E-5 0.0 0.0 0.7967222677197193 3.415158248187746E-5 80-81 6.830316496375492E-5 0.0 0.0 0.9030019924033219 3.415158248187746E-5 82-83 6.830316496375492E-5 0.0 0.0 1.0195613434139696 3.415158248187746E-5 84-85 6.830316496375492E-5 0.0 0.0 1.132961673045044 3.415158248187746E-5 86-87 6.830316496375492E-5 0.0 0.0 1.2538070476571672 3.415158248187746E-5 88-89 6.830316496375492E-5 0.0 0.0 1.3959459339467415 3.415158248187746E-5 90-91 6.830316496375492E-5 0.0 0.0 1.5600613635634035 3.415158248187746E-5 92-93 6.830316496375492E-5 0.0 0.0 1.7425674203465567 3.415158248187746E-5 94-95 6.830316496375492E-5 0.0 0.0 1.923024382180797 3.415158248187746E-5 96-97 6.830316496375492E-5 0.0 0.0 2.105291377886577 3.415158248187746E-5 98-99 6.830316496375492E-5 0.0 0.0 2.3119596792756587 3.415158248187746E-5 100-101 6.830316496375492E-5 0.0 0.0 2.5599001680940887 3.415158248187746E-5 102-103 6.830316496375492E-5 0.0 0.0 2.8342910575447333 3.415158248187746E-5 104-105 6.830316496375492E-5 0.0 0.0 3.108391658544282 3.415158248187746E-5 106-107 6.830316496375492E-5 0.0 0.0 3.3829533059073356 3.415158248187746E-5 108-109 6.830316496375492E-5 0.0 0.0 3.674232152895269 3.415158248187746E-5 110-111 6.830316496375492E-5 0.0 0.0 4.0127938658293605 3.415158248187746E-5 112-113 6.830316496375492E-5 0.0 0.0 4.380913773401518 3.415158248187746E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5285 0.0 52.082546 1 GTATCAA 9365 0.0 47.26899 1 ATCAACG 11230 0.0 38.88952 3 TCAACGC 11345 0.0 38.2888 4 TATCAAC 11480 0.0 38.072536 2 CAACGCA 11415 0.0 37.202766 5 AACGCAG 11860 0.0 36.04811 6 AACGCCG 275 0.0 34.64416 6 ACGCCGA 345 0.0 29.341347 7 ACGCAGA 14480 0.0 29.320488 7 CGCAGAG 14640 0.0 28.959372 8 GTACATG 11490 0.0 28.622942 1 TACATGG 11510 0.0 27.626354 2 ACATGGG 11835 0.0 26.193031 3 GCAGAGT 15985 0.0 25.07033 9 GAGTACT 8885 0.0 22.754234 12-13 CATGGGG 5920 0.0 22.515402 4 AGTACTT 9135 0.0 22.229298 12-13 GTACTTT 9455 0.0 21.85485 14-15 AGAGTAC 15300 0.0 21.513453 10-11 >>END_MODULE