FastQCFastQC Report
Thu 26 May 2016
SRR1364630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018626
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63580.31496671498336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA69100.044.310971
TCAACGC81250.037.0212484
GGTATCA41050.036.8592721
ATCAACG81500.036.8373453
CAACGCA81850.036.532455
AACGCAG82350.034.7648936
TATCAAC87950.034.745372
GTACATG110250.032.8511961
TACATGG112450.030.9363272
AACGCAT3850.030.919236
GTATAGG6700.030.2294561
ACGCATA3800.029.7545877
ACGCAGA96500.029.3537467
TACCTGG20100.028.7469022
CGCATAG3750.028.561578
CGCAGAG95400.028.5041858
ACATGGG117300.028.1845023
GTGTAGC12400.025.941691
CATGGGA78950.025.575884
GTACCTG24650.025.13291
Produced by FastQC (version 0.11.5)