##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364630_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018626 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.63481298665528 30.0 26.0 31.0 7.0 34.0 2 25.426944862495578 30.0 25.0 31.0 7.0 34.0 3 25.977773495436995 31.0 26.0 31.0 7.0 34.0 4 29.290915702066652 35.0 28.0 37.0 7.0 37.0 5 29.422832659442612 35.0 28.0 37.0 2.0 37.0 6 29.609504187501795 35.0 30.0 37.0 2.0 37.0 7 29.42151790376226 35.0 29.0 37.0 2.0 37.0 8 29.236668902510917 35.0 29.0 37.0 2.0 37.0 9 30.054259184217383 37.0 28.0 39.0 2.0 39.0 10-11 30.001941171866406 36.0 27.5 39.0 2.0 39.0 12-13 29.97195567678213 37.0 27.0 39.0 2.0 39.0 14-15 30.53326718272726 37.0 27.0 40.0 2.0 41.0 16-17 30.919714201640126 37.0 28.0 40.0 2.0 41.0 18-19 30.73928132303854 37.0 27.5 40.0 2.0 41.0 20-21 30.647807964427287 37.0 27.0 40.0 2.0 41.0 22-23 30.70891685730789 37.0 27.0 40.0 2.0 41.0 24-25 30.796447435037496 37.0 27.5 40.0 2.0 41.0 26-27 30.608940437703666 37.0 27.5 40.0 2.0 41.0 28-29 30.499857328697836 37.0 27.0 40.0 2.0 41.0 30-31 30.358153020916205 37.0 27.0 40.0 2.0 41.0 32-33 30.263996649205946 36.5 27.0 40.0 2.0 41.0 34-35 30.29907248792 37.0 27.0 40.0 2.0 41.0 36-37 30.21716578504389 37.0 27.0 40.0 2.0 41.0 38-39 29.930144068291995 36.0 27.0 40.0 2.0 41.0 40-41 29.789161538591102 36.0 26.5 40.0 2.0 41.0 42-43 29.279299880215554 35.5 24.5 40.0 2.0 41.0 44-45 29.134387201987884 35.0 25.0 40.0 2.0 41.0 46-47 29.074367168559206 35.0 24.5 40.0 2.0 41.0 48-49 29.000926372691126 35.0 25.0 40.0 2.0 41.0 50-51 28.76682679208531 35.0 24.0 40.0 2.0 41.0 52-53 28.656893599904095 35.0 24.0 39.5 2.0 41.0 54-55 28.471335700620124 35.0 23.5 39.0 2.0 41.0 56-57 28.245685926962203 34.5 23.0 39.0 2.0 40.5 58-59 28.034579213782045 34.0 23.0 39.0 2.0 40.0 60-61 27.73817314351445 34.0 22.0 39.0 2.0 40.0 62-63 27.55231578311188 34.0 22.0 38.5 2.0 40.0 64-65 27.323186167224637 34.0 21.5 38.0 2.0 40.0 66-67 27.063303702617524 33.0 21.0 38.0 2.0 40.0 68-69 26.842875550002823 33.0 20.5 37.0 2.0 40.0 70-71 26.421902075966525 33.0 19.0 37.0 2.0 39.5 72-73 26.182838970666186 32.5 18.5 36.0 2.0 39.0 74-75 26.151495373585796 33.0 19.5 36.0 2.0 39.0 76-77 25.912972982612928 33.0 18.0 36.0 2.0 38.5 78-79 25.62458573306794 32.0 18.0 35.0 2.0 37.5 80-81 25.23099747055671 32.0 13.0 35.0 2.0 37.0 82-83 25.34381381196913 32.0 17.0 35.0 2.0 37.0 84-85 25.182408479827366 32.0 13.0 35.0 2.0 36.0 86-87 25.059272742944955 32.0 12.5 35.0 2.0 36.0 88-89 24.89736558431329 32.0 10.5 35.0 2.0 36.0 90-91 24.771225576208767 32.0 7.0 35.0 2.0 35.0 92-93 24.64104569147529 32.0 7.0 35.0 2.0 35.0 94-95 24.536685597034815 32.0 6.0 35.0 2.0 35.0 96-97 24.422987467713188 32.0 2.0 35.0 2.0 35.0 98-99 24.33288806346495 32.0 2.0 35.0 2.0 35.0 100-101 24.22633786545898 32.0 2.0 35.0 2.0 35.0 102-103 23.140764807349157 30.0 2.0 34.0 2.0 35.0 104-105 23.943849182562793 32.0 2.0 34.5 2.0 35.0 106-107 24.092602839753376 32.0 2.0 35.0 2.0 35.0 108-109 24.044075524639034 32.0 2.0 35.0 2.0 35.0 110-111 23.981141132631798 32.0 2.0 35.0 2.0 35.0 112-113 23.891161364215066 32.0 2.0 35.0 2.0 35.0 114-115 23.78744205216816 32.0 2.0 35.0 2.0 35.0 116-117 23.679000220942363 32.0 2.0 35.0 2.0 35.0 118-119 23.5471863039513 32.0 2.0 35.0 2.0 35.0 120-121 23.36714032217954 32.0 2.0 35.0 2.0 35.0 122-123 23.156037324397882 32.0 2.0 35.0 2.0 35.0 124-125 22.232558928697046 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2237142484425014 1101 2 1.5780955597758926 1101 3 1.1181332508802129 1101 4 1.0842430282846784 1101 5 0.9557534183890155 1101 6 0.8341863055735317 1101 7 0.9252625368481908 1101 8 1.1770909172973525 1101 9 1.5267469295121217 1101 10-11 1.572131051577692 1101 12-13 1.5659076257938587 1101 14-15 1.8283191284620735 1101 16-17 1.4012914310301419 1101 18-19 1.3847868735675135 1101 20-21 1.5251390954530955 1101 22-23 1.3002177464807971 1101 24-25 1.3656353260121499 1101 26-27 1.6102292958187263 1101 28-29 1.6545151608721937 1101 30-31 1.7470404922036629 1101 32-33 1.6659993685118586 1101 34-35 1.54479185870834 1101 36-37 1.379151123570562 1101 38-39 1.321932487177211 1101 40-41 1.3451494779909794 1101 42-43 1.3668552953078859 1101 44-45 1.3193247324243096 1101 46-47 0.9997883650573378 1101 48-49 0.8246515940672836 1101 50-51 0.9175744842822411 1101 52-53 0.795258555706365 1101 54-55 0.8180659381432456 1101 56-57 0.850103479071123 1101 58-59 0.7668616407488145 1101 60-61 0.8516485554367925 1101 62-63 0.5990762035115544 1101 64-65 0.6414875265430879 1101 66-67 0.7198955288055693 1101 68-69 0.5732167719356411 1101 70-71 0.5777330923766186 1101 72-73 0.5003289134603754 1101 74-75 0.418737779917997 1101 76-77 0.4913359999295537 1101 78-79 0.4583514128661932 1101 80-81 0.5714504152724942 1101 82-83 0.5804767952613901 1101 84-85 0.6783693276112501 1101 86-87 0.6045849699221435 1101 88-89 0.5159115912062582 1101 90-91 0.6802769227894565 1101 92-93 0.7279627654784093 1101 94-95 0.6884906826827368 1101 96-97 0.6534557028031678 1101 98-99 0.617801207750464 1101 100-101 0.5236960394656336 1101 102-103 0.4401708226956771 1101 104-105 0.6236426920072482 1101 106-107 0.5849037220094111 1101 108-109 0.5431983251784871 1101 110-111 0.6748020708587745 1101 112-113 0.5709371742225926 1101 114-115 0.6456450942434913 1101 116-117 0.5468986825083029 1101 118-119 0.6425315012236119 1101 120-121 0.6508157604308309 1101 122-123 0.5562317709020377 1101 124-125 0.46195873278796284 1102 1 0.8379084830259984 1102 2 1.229485105718883 1102 3 0.879966865390017 1102 4 0.8697546285977253 1102 5 0.6278370403078277 1102 6 0.29381225488712914 1102 7 0.32742091841326015 1102 8 0.5529979172194217 1102 9 1.1939672778343358 1102 10-11 1.0567301912545268 1102 12-13 1.1602740347733587 1102 14-15 1.3808204648277957 1102 16-17 1.1915001236089502 1102 18-19 1.0032548441466105 1102 20-21 1.2815364729931567 1102 22-23 1.1693558207750492 1102 24-25 1.0529782687388582 1102 26-27 1.4391450120284333 1102 28-29 1.035744189260221 1102 30-31 1.0314664257113755 1102 32-33 1.2993020413530658 1102 34-35 1.2676487616547085 1102 36-37 1.0885651203413325 1102 38-39 1.3110070525427808 1102 40-41 1.41222845144258 1102 42-43 1.6326513576664965 1102 44-45 1.1712051938244699 1102 46-47 1.032746272006765 1102 48-49 0.8306353842529255 1102 50-51 0.9820503709759905 1102 52-53 0.9225038970860631 1102 54-55 0.8867317567070394 1102 56-57 0.8124228501724744 1102 58-59 0.9419979939576812 1102 60-61 0.9705789579490549 1102 62-63 0.8699970500484753 1102 64-65 0.6389018372363182 1102 66-67 0.7097663705677952 1102 68-69 0.6347254327929157 1102 70-71 0.7288251803232058 1102 72-73 0.37020915094381834 1102 74-75 0.7064638507393397 1102 76-77 0.539119498803931 1102 78-79 0.611709230687989 1102 80-81 0.7508535345202247 1102 82-83 0.8152532023419887 1102 84-85 0.7375280702969107 1102 86-87 0.7910949204958193 1102 88-89 0.7223657162681398 1102 90-91 0.7373990660676171 1102 92-93 0.8454687722639669 1102 94-95 0.7120910659754784 1102 96-97 0.5950247992223687 1102 98-99 0.6930538000804383 1102 100-101 0.7533309783769795 1102 102-103 0.7248200143453403 1102 104-105 0.7611733845136079 1102 106-107 0.7813131515376597 1102 108-109 0.8344099231865059 1102 110-111 0.7056920959176907 1102 112-113 0.48185426686155 1102 114-115 0.5573147226844313 1102 116-117 0.6983051868499821 1102 118-119 0.7039128917684003 1102 120-121 0.741499288172907 1102 122-123 0.5027617751937754 1102 124-125 0.4780804073776963 1103 1 0.47347998937607017 1103 2 0.880562339910373 1103 3 0.502907120281737 1103 4 0.3050983546391244 1103 5 0.4194336136030863 1103 6 0.3620141237967829 1103 7 0.3639102127387517 1103 8 0.5652158307517929 1103 9 1.0107219084413437 1103 10-11 0.9934733772899271 1103 12-13 0.8584573479212807 1103 14-15 1.1254848499876324 1103 16-17 0.7869578509170303 1103 18-19 0.7493675787325458 1103 20-21 1.0202474586707773 1103 22-23 0.8490039279762378 1103 24-25 0.7284741075815155 1103 26-27 0.9774843269097104 1103 28-29 0.9521771496264506 1103 30-31 0.9244085695129733 1103 32-33 0.9925702998892731 1103 34-35 0.8248271747481901 1103 36-37 0.5947218554161644 1103 38-39 0.6976235705040956 1103 40-41 0.9307847687349948 1103 42-43 1.1393447653710282 1103 44-45 0.876686365837493 1103 46-47 0.6583104449420212 1103 48-49 0.4529956327001301 1103 50-51 0.5055150761393783 1103 52-53 0.38680516710017443 1103 54-55 0.3651720727105534 1103 56-57 0.3816613972674432 1103 58-59 0.4011353177057231 1103 60-61 0.5084549343487907 1103 62-63 0.2753976656108712 1103 64-65 0.09787527027446785 1103 66-67 0.17610821881055116 1103 68-69 0.14266774326303278 1103 70-71 0.3189950710997671 1103 72-73 0.20807123830034158 1103 74-75 0.15494827834409364 1103 76-77 0.2522574225586425 1103 78-79 0.32694717777629023 1103 80-81 0.4043924627305344 1103 82-83 0.4299511434302232 1103 84-85 0.48858808207481985 1103 86-87 0.43785766230406153 1103 88-89 0.3108700329827663 1103 90-91 0.3757739368995203 1103 92-93 0.31485843214907305 1103 94-95 0.26330219054353066 1103 96-97 0.22421567912103413 1103 98-99 0.1381526345119326 1103 100-101 0.17342509053377952 1103 102-103 0.15606369483067795 1103 104-105 0.11166227883465751 1103 106-107 0.3372921068593868 1103 108-109 0.38536326711254887 1103 110-111 0.2888480198724004 1103 112-113 0.317222993180728 1103 114-115 0.306620270523851 1103 116-117 0.3056274904311316 1103 118-119 0.3865638101119373 1103 120-121 0.34836380888980045 1103 122-123 0.14202832259985598 1103 124-125 0.007631291276403829 1104 1 0.6995158950374112 1104 2 1.109381712895214 1104 3 0.5998301921790841 1104 4 0.7353800225417011 1104 5 0.15261656870413276 1104 6 0.3583737945544776 1104 7 0.12577882288890052 1104 8 0.6960875780466722 1104 9 0.8436119472173687 1104 10-11 0.9098097780778645 1104 12-13 0.8087407362989296 1104 14-15 0.7929530075875029 1104 16-17 0.5367638619084723 1104 18-19 0.6594947523295716 1104 20-21 0.9158075985301544 1104 22-23 0.8007502646165889 1104 24-25 0.6299741876162699 1104 26-27 0.8877435306346477 1104 28-29 0.8428198341773445 1104 30-31 0.9688329780452243 1104 32-33 0.9375105787151234 1104 34-35 0.835749850282923 1104 36-37 0.7904077641808982 1104 38-39 0.8724403344647165 1104 40-41 0.7669912409879771 1104 42-43 1.0481049235240647 1104 44-45 0.7737027027282863 1104 46-47 0.6208376812601593 1104 48-49 0.6569638866683825 1104 50-51 0.5381525426418428 1104 52-53 0.4851367418466488 1104 54-55 0.42884788947342045 1104 56-57 0.5250398549595445 1104 58-59 0.4775798361959325 1104 60-61 0.5008380209899563 1104 62-63 0.3797824814383688 1104 64-65 0.2677384816174069 1104 66-67 0.37407359370533655 1104 68-69 0.32311100515309477 1104 70-71 0.3391511987677305 1104 72-73 0.37590167568212607 1104 74-75 0.270536708040364 1104 76-77 0.32893028511337263 1104 78-79 0.28489571814919756 1104 80-81 0.5137146064424734 1104 82-83 0.4683454501822162 1104 84-85 0.4848835072445006 1104 86-87 0.34827464883753123 1104 88-89 0.3520326712332853 1104 90-91 0.2683890698933311 1104 92-93 0.37369944174126957 1104 94-95 0.3317956641718034 1104 96-97 0.27811620299480566 1104 98-99 0.26917797231850216 1104 100-101 0.40737850268229536 1104 102-103 0.31406374056482633 1104 104-105 0.3579410981531943 1104 106-107 0.4344235413544766 1104 108-109 0.4483896589823253 1104 110-111 0.4389723187515848 1104 112-113 0.4050032645584487 1104 114-115 0.5259887908390688 1104 116-117 0.3798104008869423 1104 118-119 0.35390527323442456 1104 120-121 0.4369826485273691 1104 122-123 0.4837390083833988 1104 124-125 0.3335923344940852 1105 1 0.6593581331201506 1105 2 1.0827691618162376 1105 3 0.6443045737174948 1105 4 0.6995542407502775 1105 5 0.4052997310697499 1105 6 0.4599065186796025 1105 7 0.3226796239021432 1105 8 0.5012315250438206 1105 9 0.7089134569748907 1105 10-11 0.5999765868027147 1105 12-13 0.7509867747188324 1105 14-15 0.9516307287326917 1105 16-17 0.5447012702883605 1105 18-19 0.6906034290655256 1105 20-21 0.6737486086835531 1105 22-23 0.7004974413028613 1105 24-25 0.4228751238019548 1105 26-27 0.7343573444530413 1105 28-29 0.9044606390508392 1105 30-31 0.8854840193709101 1105 32-33 0.8120799284276288 1105 34-35 0.643976014964057 1105 36-37 0.2922061085091734 1105 38-39 0.6754937167736941 1105 40-41 0.7469225297427968 1105 42-43 0.9533253128673067 1105 44-45 0.7419522217326247 1105 46-47 0.2887031028996567 1105 48-49 0.31427552927414126 1105 50-51 0.4291316784153558 1105 52-53 0.34704930053172234 1105 54-55 0.35933932816371694 1105 56-57 0.5211887645649895 1105 58-59 0.4564509323236656 1105 60-61 0.3808035159996983 1105 62-63 0.30507476006224366 1105 64-65 0.11361165427350528 1105 66-67 0.05378143478746367 1105 68-69 -0.11876097849400935 1105 70-71 0.2874997538315398 1105 72-73 0.11413849525679254 1105 74-75 0.12109806562931169 1105 76-77 0.22125743759148975 1105 78-79 0.254729381892286 1105 80-81 0.48659733036632247 1105 82-83 0.46446204976055583 1105 84-85 0.4126707026006713 1105 86-87 0.4333154876524503 1105 88-89 0.5176693895769269 1105 90-91 0.37949456647513813 1105 92-93 0.4514761919542494 1105 94-95 0.2534150872227734 1105 96-97 0.28794517807625297 1105 98-99 0.4349047879672341 1105 100-101 0.5162957629530176 1105 102-103 0.2724367198904325 1105 104-105 0.4202400212017281 1105 106-107 0.3653734646261064 1105 108-109 0.4667375699642804 1105 110-111 0.5057426847150808 1105 112-113 0.24264654363848237 1105 114-115 0.29594770095935274 1105 116-117 0.34239332377699583 1105 118-119 0.2735806919988626 1105 120-121 0.2119399728079614 1105 122-123 0.19005977131530116 1105 124-125 0.13500685688726577 1106 1 0.09176972795534155 1106 2 -2.1648814566917807 1106 3 -0.30117586968814436 1106 4 0.2130877174980199 1106 5 0.2121044798079943 1106 6 0.23977916290485268 1106 7 0.4712999431892051 1106 8 0.5814348317093057 1106 9 0.90039777967662 1106 10-11 1.0803753680061838 1106 12-13 1.0419468707173394 1106 14-15 1.206440873073479 1106 16-17 0.7983869713048506 1106 18-19 0.8734565439126953 1106 20-21 1.0832071196673247 1106 22-23 0.6173826085104608 1106 24-25 0.82701018106604 1106 26-27 0.7754337001988318 1106 28-29 0.8934535009899278 1106 30-31 0.943647374175324 1106 32-33 1.0447783828919128 1106 34-35 0.8091136913589558 1106 36-37 0.5841400852970757 1106 38-39 0.9982864915795879 1106 40-41 0.8773962122127728 1106 42-43 1.154054339537005 1106 44-45 1.098218817246682 1106 46-47 0.8081889349240434 1106 48-49 0.6516678375210461 1106 50-51 0.5747373064552335 1106 52-53 0.5559699150487027 1106 54-55 0.5123440880349612 1106 56-57 0.5165388368370571 1106 58-59 0.5125020467637178 1106 60-61 0.5872464216799749 1106 62-63 0.3994801910767727 1106 64-65 0.42947341732586963 1106 66-67 0.4135504065398372 1106 68-69 0.31906444866299566 1106 70-71 0.3899879589104458 1106 72-73 0.2128527261277 1106 74-75 0.33245345914698987 1106 76-77 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-2.1676346709468604 2216 122-123 -2.0025824298706674 2216 124-125 -1.9099560793883654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 216356.0 3 28832.0 4 17326.0 5 13931.0 6 13090.0 7 14297.0 8 15446.0 9 16198.0 10 17428.0 11 18343.0 12 18986.0 13 19437.0 14 19509.0 15 16726.0 16 13668.0 17 12042.0 18 10995.0 19 9971.0 20 9733.0 21 10362.0 22 13020.0 23 18491.0 24 27343.0 25 35528.0 26 39532.0 27 41179.0 28 47712.0 29 53543.0 30 61518.0 31 71146.0 32 84508.0 33 105309.0 34 136279.0 35 183186.0 36 257009.0 37 268383.0 38 62178.0 39 86.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0480936058042 19.21976960938838 14.177883791988204 22.554252992819222 2 13.51394837796566 21.054291283350093 41.11731039020696 24.314449948477286 3 19.109588258354755 26.984637262204007 27.63941315623545 26.26636132320579 4 11.70118375530822 17.62775992639534 36.40862311194307 34.26243320635337 5 13.321601639587858 39.750016524095614 32.39040797370855 14.537973862607984 6 31.055038286618576 37.464031036467254 17.61815075210203 13.862779924812141 7 28.39892099243672 34.098515793618205 19.941034907870765 17.561528306074308 8 25.257595576062485 37.77606794755013 19.07321686080134 17.893119615586038 9 25.93079059867244 18.858052820418123 19.509571491826357 35.701585089083075 10-11 23.903582588092533 29.388528405636556 26.488346313293587 20.219542692977328 12-13 24.847793281715294 26.400775709648784 27.041088494516885 21.710342514119038 14-15 22.073997566519317 27.27156090935644 25.11890081915933 25.53554070496491 16-17 22.091360770822547 30.788990106457486 25.122239668394275 21.99740945432569 18-19 22.029154255597668 28.958234534896043 27.5499550955674 21.462656113938884 20-21 23.01623204128968 27.897035858982015 27.5522851983526 21.534446901375702 22-23 22.722008574904827 27.828008476616585 27.19712700479478 22.252855943683812 24-25 22.6191454125855 27.79035897059178 27.32649142561032 22.264004191212404 26-27 22.392127864348733 27.945992171367784 27.406817723578364 22.255062240705115 28-29 22.121285205134388 27.820926615711834 27.76142637800863 22.296361801145142 30-31 22.12864953929346 27.652662765489538 27.76947977607055 22.449207919146456 32-33 22.117158826123568 27.744694706606587 27.574843916703625 22.56330255056622 34-35 22.811662928294197 27.663509763676313 27.237975315330605 22.286851992698885 36-37 22.657230836094534 27.439230256112445 27.220781914084757 22.68275699370826 38-39 22.373481714817608 27.612286784953966 27.585163803699096 22.429067696529327 40-41 22.535164073360985 27.437222923682125 27.74522270228169 22.282390300675196 42-43 22.362910592666147 27.414280157980723 27.673624064094355 22.549185185258775 44-45 22.456375062929233 27.735408668441046 27.17617281601143 22.63204345261829 46-47 22.455642799535298 27.905970003738556 26.764780379109837 22.87360681761631 48-49 22.671016398322955 27.820958098687637 26.78257957280012 22.725445930189288 50-51 22.54520689668005 27.868015833733395 26.728245053198812 22.85853221638774 52-53 22.900355898379125 27.875166075157043 26.56976695132912 22.65471107513471 54-55 22.556135957456785 27.722437932794406 26.795322191701015 22.92610391804779 56-57 22.569231047883562 28.151011033448203 26.621279725167422 22.658478193500812 58-59 22.790720403077653 27.5873633409089 26.687647064951918 22.934269191061528 60-61 22.842118868626184 27.672891847377514 26.929879205246888 22.555110078749415 62-63 22.637796105694015 27.77760827390569 26.85730147024351 22.727294150156784 64-65 22.627657925252535 27.463633939589364 26.804710317927853 23.10399781723024 66-67 22.51988489318543 28.066871672468878 26.50586632560209 22.9073771087436 68-69 22.607912125632883 27.77949462516537 26.764325245080656 22.848268004121095 70-71 22.461105982508293 27.86881667838171 26.83172555355427 22.838351785555727 72-73 22.757955115212354 27.689598379692193 26.660961799874727 22.891484705220723 74-75 22.51312886246943 27.777047638838127 26.626954823025066 23.082868675667378 76-77 22.69643792983799 27.739820734507852 26.792177478740157 22.771563856914003 78-79 22.7327194369516 27.758038011967876 26.67797724242706 22.83126530865346 80-81 22.386180352178954 28.210632643108358 26.72960171619395 22.673585288518737 82-83 22.713324558352713 28.038543763355346 26.498018683465148 22.750112994826797 84-85 22.65851682708883 27.715101543932093 26.536949847382736 23.089431781596335 86-87 22.70625619945844 27.63007139890547 26.93806421584511 22.72560818579098 88-89 22.692229067265785 27.635942697458777 26.730542897517523 22.941285337757915 90-91 22.50536615762277 27.965067200690203 26.88746982040209 22.642096821284934 92-93 22.546999116402226 27.780616712549914 26.84428089975159 22.828103271296268 94-95 22.514107836871126 27.71207408524845 26.737303443046617 23.0365146348338 96-97 22.67537805527908 27.71778554225715 26.710117760535386 22.896718641928388 98-99 22.370574998691442 27.678629237214032 26.65334046776149 23.297455296333037 100-101 22.219273532753476 27.697924786758083 26.963114126931494 23.11968755355695 102-103 22.649580390100862 27.577214647776056 26.784716041596003 22.98848892052708 104-105 22.367927960728398 27.607357398506906 27.036552122842554 22.988162517922145 106-107 22.303622717509388 27.78790013343982 27.042522161427456 22.865954987623333 108-109 22.366851106115774 27.927172132957146 26.493842950462305 23.212133810464778 110-111 22.02035352317919 27.876604161768746 26.977941256704568 23.125101058347493 112-113 22.34306512870311 27.76745657739297 26.945598622458643 22.943879671445277 114-115 21.933068171673987 28.01131235183844 26.832591079062478 23.22302839742509 116-117 21.867907024725245 27.87413278632465 26.985574315720456 23.27238587322965 118-119 22.05162522667981 28.036592084442663 26.57887416065865 23.33290852821888 120-121 21.837161476185106 27.99522995508869 26.85371655669201 23.3138920120342 122-123 21.940913411570865 27.97391443006571 26.820190649736226 23.2649815086272 124-125 21.872110257866982 27.836847995441133 26.86124501343184 23.429796733260048 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6741.0 1 4861.0 2 2316.5 3 1389.0 4 1009.5 5 851.0 6 864.5 7 1008.0 8 1198.0 9 1306.0 10 1257.5 11 1213.0 12 1310.5 13 1478.0 14 1565.0 15 1677.0 16 1831.0 17 2005.5 18 2267.0 19 2580.0 20 3008.5 21 3609.5 22 4446.5 23 5411.5 24 6689.0 25 8290.5 26 10444.5 27 13107.0 28 15656.0 29 18542.0 30 22019.5 31 26038.5 32 30228.5 33 34464.0 34 39102.0 35 42768.0 36 46922.5 37 52639.5 38 56635.5 39 58099.5 40 58656.5 41 61281.5 42 64838.5 43 67925.0 44 70538.5 45 72007.5 46 71942.5 47 72114.0 48 76061.0 49 78267.0 50 77728.5 51 75858.0 52 72260.5 53 70438.0 54 68269.0 55 63010.0 56 56777.0 57 51000.0 58 45573.0 59 40443.0 60 35100.5 61 28965.5 62 23183.0 63 18369.0 64 14487.5 65 11640.5 66 9116.5 67 7165.5 68 5355.5 69 3855.0 70 2937.5 71 2241.0 72 1788.0 73 1489.5 74 1223.0 75 989.0 76 671.0 77 429.5 78 311.0 79 236.0 80 161.5 81 105.5 82 79.5 83 64.0 84 46.5 85 29.5 86 23.5 87 18.5 88 14.0 89 10.5 90 9.0 91 6.0 92 5.0 93 6.5 94 5.0 95 4.0 96 5.0 97 3.5 98 1.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.20910262723258297 2 0.24526583923916565 3 0.29866850025710556 4 0.28286567199669477 5 0.31769134054549975 6 0.23466456887011267 7 0.2255494578985904 8 0.20464414903999056 9 0.20320752828904415 10-11 0.14319145795209218 12-13 0.17036340560361354 14-15 0.1890642446892094 16-17 0.15953921132493093 18-19 0.1878257785246004 20-21 0.18287191386616442 22-23 0.2052386127990029 24-25 0.19490980498616384 26-27 0.24729692374912438 28-29 0.1737815722179344 30-31 0.15260380080312055 32-33 0.19401810934764538 34-35 0.15074610155620705 36-37 0.1509194868192523 38-39 0.1847543824363701 40-41 0.20689815745957896 42-43 0.27092685816986406 44-45 0.1716761797380991 46-47 0.15562565824476648 48-49 0.1573347415519269 50-51 0.1638986122243546 52-53 0.18453145852674047 54-55 0.17098263868591806 56-57 0.1701900203405683 58-59 0.14539592772509619 60-61 0.15765674275472524 62-63 0.1456188516347258 64-65 0.14061544832970543 66-67 0.1280821707438624 68-69 0.13162418397464412 70-71 0.13370480713118726 72-73 0.10992625677069452 74-75 0.1123041118067438 76-77 0.1326397262296235 78-79 0.13937698216509645 80-81 0.14876455569283265 82-83 0.1509442561425445 84-85 0.13865867178962324 86-87 0.16491415447933397 88-89 0.15941536470847 90-91 0.14784809073102198 92-93 0.14876455569283265 94-95 0.17011571237069176 96-97 0.15753289613826435 98-99 0.15096902546583665 100-101 0.13199572382402683 102-103 0.17241925943686448 104-105 0.12951879149480883 106-107 0.13553773705480857 108-109 0.14708024170896442 110-111 0.12206322518386269 112-113 0.11294811421234047 114-115 0.11126380022847224 116-117 0.1152516612785132 118-119 0.09982037286748512 120-121 0.09281065437579819 122-123 0.11966060082452122 124-125 0.11564797045118809 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2018626.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.70123940430492 #Duplication Level Percentage of deduplicated Percentage of total 1 83.39167401727333 53.12152990900082 2 9.472450173800112 12.068136325331814 3 2.9744523491908046 5.684289035775019 4 1.3764573154488144 3.507281479248472 5 0.7427282924791395 2.365635638578213 6 0.45664063805509203 1.7453144763889186 7 0.2939344601664667 1.3106792591367455 8 0.2021718118796116 1.0302875991476188 9 0.14658561774257703 0.8403916976143042 >10 0.8393313727907158 9.75832901723402 >50 0.06294607785095091 2.748879383459369 >100 0.03786623956989475 4.164671608119129 >500 0.002274286619723801 0.9519314202459057 >1k 4.061226106649635E-4 0.373673699152947 >5k 8.12245221329927E-5 0.3289694515666625 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6358 0.31496671498336 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 4.953864658435986E-5 0.0 0.0 0.0 16-17 0.0 4.953864658435986E-5 0.0 7.430796987653979E-5 0.0 18-19 0.0 4.953864658435986E-5 0.0 9.907729316871972E-5 0.0 20-21 0.0 4.953864658435986E-5 0.0 1.2384661646089965E-4 0.0 22-23 0.0 4.953864658435986E-5 0.0 2.476932329217993E-4 0.0 24-25 0.0 4.953864658435986E-5 0.0 3.46770526090519E-4 0.0 26-27 0.0 4.953864658435986E-5 0.0 6.687717288888581E-4 2.476932329217993E-5 28-29 0.0 4.953864658435986E-5 0.0 0.001065080901563737 4.953864658435986E-5 30-31 0.0 4.953864658435986E-5 0.0 0.002427393682633633 4.953864658435986E-5 32-33 0.0 9.907729316871972E-5 0.0 0.004582324809053287 4.953864658435986E-5 34-35 0.0 9.907729316871972E-5 0.0 0.007653720897283597 4.953864658435986E-5 36-37 0.0 9.907729316871972E-5 0.0 0.01384605172032858 4.953864658435986E-5 38-39 0.0 9.907729316871972E-5 0.0 0.024843631262056465 4.953864658435986E-5 40-41 0.0 9.907729316871972E-5 0.0 0.03824383516312581 4.953864658435986E-5 42-43 0.0 9.907729316871972E-5 0.0 0.05186696297382477 4.953864658435986E-5 44-45 0.0 9.907729316871972E-5 0.0 0.06734779003143723 4.953864658435986E-5 46-47 0.0 9.907729316871972E-5 0.0 0.08698986340213591 4.953864658435986E-5 48-49 0.0 9.907729316871972E-5 0.0 0.10878686789925424 4.953864658435986E-5 50-51 0.0 9.907729316871972E-5 0.0 0.13472034938616662 4.953864658435986E-5 52-53 0.0 9.907729316871972E-5 0.0 0.15901905553579515 4.953864658435986E-5 54-55 0.0 9.907729316871972E-5 0.0 0.18418468800064997 4.953864658435986E-5 56-57 0.0 9.907729316871972E-5 0.0 0.21442803174040165 9.907729316871972E-5 58-59 2.476932329217993E-5 9.907729316871972E-5 0.0 0.253712178481799 9.907729316871972E-5 60-61 4.953864658435986E-5 9.907729316871972E-5 0.0 0.2965383384539781 9.907729316871972E-5 62-63 4.953864658435986E-5 9.907729316871972E-5 0.0 0.3434266674460747 9.907729316871972E-5 64-65 4.953864658435986E-5 9.907729316871972E-5 0.0 0.38902699162697796 1.4861593975307958E-4 66-67 4.953864658435986E-5 9.907729316871972E-5 0.0 0.4416618036228603 1.4861593975307958E-4 68-69 9.907729316871972E-5 9.907729316871972E-5 0.0 0.5071023557607997 1.4861593975307958E-4 70-71 9.907729316871972E-5 9.907729316871972E-5 0.0 0.5830203316513312 1.4861593975307958E-4 72-73 1.2384661646089965E-4 9.907729316871972E-5 0.0 0.6620840115999695 1.4861593975307958E-4 74-75 1.4861593975307958E-4 9.907729316871972E-5 0.0 0.7425595429762621 1.4861593975307958E-4 76-77 1.4861593975307958E-4 9.907729316871972E-5 0.0 0.831060335099221 1.4861593975307958E-4 78-79 1.4861593975307958E-4 9.907729316871972E-5 0.0 0.9357602646552655 1.4861593975307958E-4 80-81 1.4861593975307958E-4 9.907729316871972E-5 0.0 1.0581454910419266 1.4861593975307958E-4 82-83 1.4861593975307958E-4 9.907729316871972E-5 0.0 1.1961849297492453 1.4861593975307958E-4 84-85 1.4861593975307958E-4 9.907729316871972E-5 0.0 1.3270164953785397 1.4861593975307958E-4 86-87 1.4861593975307958E-4 9.907729316871972E-5 0.0 1.4642633157405087 1.4861593975307958E-4 88-89 1.4861593975307958E-4 9.907729316871972E-5 0.0 1.6280628506716945 1.4861593975307958E-4 90-91 1.4861593975307958E-4 9.907729316871972E-5 0.0 1.8168298634813977 1.4861593975307958E-4 92-93 1.4861593975307958E-4 9.907729316871972E-5 0.0 2.020730932822623 1.4861593975307958E-4 94-95 1.4861593975307958E-4 9.907729316871972E-5 0.0 2.2299821759949587 1.4861593975307958E-4 96-97 1.4861593975307958E-4 9.907729316871972E-5 0.0 2.439530651046801 1.4861593975307958E-4 98-99 1.4861593975307958E-4 9.907729316871972E-5 0.0 2.6719412114973253 1.4861593975307958E-4 100-101 1.4861593975307958E-4 9.907729316871972E-5 0.0 2.952206104548341 1.4861593975307958E-4 102-103 1.4861593975307958E-4 9.907729316871972E-5 0.0 3.2599154078070924 1.4861593975307958E-4 104-105 1.4861593975307958E-4 9.907729316871972E-5 0.0 3.5616553041524286 1.4861593975307958E-4 106-107 1.4861593975307958E-4 9.907729316871972E-5 0.0 3.8649556678651718 1.4861593975307958E-4 108-109 1.4861593975307958E-4 9.907729316871972E-5 0.0 4.188096259534952 1.4861593975307958E-4 110-111 1.4861593975307958E-4 9.907729316871972E-5 0.0 4.56951906891123 1.4861593975307958E-4 112-113 1.4861593975307958E-4 9.907729316871972E-5 0.0 4.992108493599112 1.4861593975307958E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6910 0.0 44.31097 1 TCAACGC 8125 0.0 37.021248 4 GGTATCA 4105 0.0 36.859272 1 ATCAACG 8150 0.0 36.837345 3 CAACGCA 8185 0.0 36.53245 5 AACGCAG 8235 0.0 34.764893 6 TATCAAC 8795 0.0 34.74537 2 GTACATG 11025 0.0 32.851196 1 TACATGG 11245 0.0 30.936327 2 AACGCAT 385 0.0 30.91923 6 GTATAGG 670 0.0 30.229456 1 ACGCATA 380 0.0 29.754587 7 ACGCAGA 9650 0.0 29.353746 7 TACCTGG 2010 0.0 28.746902 2 CGCATAG 375 0.0 28.56157 8 CGCAGAG 9540 0.0 28.504185 8 ACATGGG 11730 0.0 28.184502 3 GTGTAGC 1240 0.0 25.94169 1 CATGGGA 7895 0.0 25.57588 4 GTACCTG 2465 0.0 25.1329 1 >>END_MODULE