FastQCFastQC Report
Thu 26 May 2016
SRR1364630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018626
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99120.4910270649441749No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84240.41731355882664745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59330.29391279018500704No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24610.12191460924410961No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21600.10700347662221728No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63200.052.8914641
GTATCAA107900.047.325931
ATCAACG140300.036.046723
TCAACGC141350.035.697424
TATCAAC141350.035.6335372
CAACGCA142600.034.9218525
AACGCAG145850.034.2568636
GTACATG115000.030.9844061
ACGCAGA170850.029.0008777
TACATGG120750.028.7945332
CGCAGAG173050.028.5289468
ACATGGG120000.028.0137793
GCAGAGT182250.026.0438029
GAGTACT103650.025.7082612-13
CATGGGA81650.024.5589894
TATCACG1701.3927853E-524.5150892
AGTACTT108900.024.33218212-13
GTACTTT112000.024.08462514-15
TACCTGG18600.023.686622
AGAGTAC173300.023.0175710-11
Produced by FastQC (version 0.11.5)