FastQCFastQC Report
Thu 26 May 2016
SRR1364629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3983158
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70960.1781501010002616No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128250.046.0397381
ATCAACG162700.035.8518643
GGTATCA76000.035.827721
GTACATG224800.035.5425871
TCAACGC162500.035.492664
CAACGCA164050.035.2676055
TATCAAC169850.035.1845472
AACGCAG164150.034.1201866
TACATGG228200.033.811992
ACGCATA5800.031.807197
CGCATAG5750.031.0449058
ACATGGG240800.030.3602033
ACGCAGA189900.029.1440287
CGCAGAG185150.028.1525258
CATGGGA158200.027.1923854
GTTATCA23800.027.0373041
AACGCAT7500.026.9823846
ATGGGAG61800.024.1037455
GCAGAGT204000.023.9168649
ATGGGAT60850.023.8925385
Produced by FastQC (version 0.11.5)