FastQCFastQC Report
Thu 26 May 2016
SRR1364629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3983158
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167810.42129887892973367No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140020.35153011755998637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98340.24688952835915623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42150.10582055745717343No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA112250.057.594241
GTATCAA195800.051.855231
ATCAACG259000.039.0566863
TATCAAC257700.039.0502742
TCAACGC260400.038.8019754
CAACGCA262250.037.957095
AACGCAG268250.037.09416
GTACATG221750.032.2174531
ACGCAGA315900.031.2726677
TACATGG227500.030.9376872
CGCAGAG318000.030.8414388
ACATGGG244300.027.9374143
GCAGAGT344100.027.5676179
GAGTACT196450.025.61308712-13
AGTACTT203250.024.4924912-13
CATGGGA157250.024.4076634
GTACTTT208500.024.01857214-15
AGAGTAC326300.023.10665310-11
CATGGGG91350.023.0596054
CAGAGTA341300.022.80683910-11
Produced by FastQC (version 0.11.5)