##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364629_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3983158 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.518608601516686 31.0 30.0 33.0 26.0 34.0 2 29.60849808117077 31.0 30.0 34.0 26.0 34.0 3 29.705393308525547 31.0 30.0 34.0 26.0 34.0 4 33.40670995225396 35.0 33.0 37.0 28.0 37.0 5 32.75087405520946 35.0 33.0 37.0 26.0 37.0 6 32.76577705428708 35.0 33.0 37.0 25.0 37.0 7 32.555523782887846 35.0 33.0 37.0 25.0 37.0 8 32.49643097261018 35.0 33.0 37.0 24.0 37.0 9 33.76019957029071 38.0 33.0 39.0 23.0 39.0 10-11 33.56535480139126 37.0 33.0 39.0 20.5 39.0 12-13 33.42784908356636 37.0 33.0 39.0 19.0 39.0 14-15 34.396648212297876 38.0 33.0 41.0 19.0 41.0 16-17 34.31651204898224 38.0 33.0 41.0 18.5 41.0 18-19 34.36960823547547 38.5 33.0 41.0 18.5 41.0 20-21 34.39539204821902 39.0 33.0 41.0 19.0 41.0 22-23 34.383385871210734 39.0 33.0 41.0 19.0 41.0 24-25 34.369935990487946 39.0 33.0 41.0 19.5 41.0 26-27 34.26443716267344 39.0 33.0 41.0 19.0 41.0 28-29 34.01633728815176 38.0 33.0 41.0 18.0 41.0 30-31 33.925188757262454 38.0 33.0 41.0 17.5 41.0 32-33 33.712236998883796 38.0 33.0 40.0 16.5 41.0 34-35 33.5479870494718 38.0 33.0 40.0 16.0 41.0 36-37 33.355319573062374 38.0 32.0 40.0 15.0 41.0 38-39 33.197637276753774 38.0 32.0 40.0 10.5 41.0 40-41 32.99242912282164 38.0 32.0 40.0 9.0 41.0 42-43 32.7759183793362 38.0 31.5 40.0 7.5 41.0 44-45 32.53174440983762 38.0 31.0 40.0 2.0 41.0 46-47 32.215178132527 38.0 31.0 40.0 2.0 41.0 48-49 31.909930261365478 38.0 30.0 40.0 2.0 41.0 50-51 31.689580478605166 37.0 30.0 40.0 2.0 41.0 52-53 31.503804393398404 37.0 30.0 40.0 2.0 41.0 54-55 31.278016588847343 37.0 30.0 40.0 2.0 41.0 56-57 31.048648961452194 36.5 29.0 40.0 2.0 41.0 58-59 30.840799184968308 36.0 29.0 40.0 2.0 41.0 60-61 30.62281059400606 36.0 29.0 40.0 2.0 41.0 62-63 30.348712629526624 35.5 28.5 39.0 2.0 41.0 64-65 30.065072613238037 35.0 28.0 39.0 2.0 41.0 66-67 29.78215576685635 35.0 28.0 39.0 2.0 40.5 68-69 29.46504406804852 35.0 28.0 38.0 2.0 40.0 70-71 29.209068156472828 35.0 28.0 37.5 2.0 40.0 72-73 29.055176570951993 35.0 28.0 37.0 2.0 39.5 74-75 28.776138681920223 34.0 28.0 36.5 2.0 39.0 76-77 28.490186932077513 34.0 27.0 36.0 2.0 39.0 78-79 28.203914331291905 34.0 27.0 36.0 2.0 38.5 80-81 27.949510162539372 34.0 26.5 35.0 2.0 37.0 82-83 27.71186769392527 34.0 26.0 35.0 2.0 37.0 84-85 27.709597259260114 34.0 27.0 35.0 2.0 36.5 86-87 27.573347705514067 34.0 27.0 35.0 2.0 36.0 88-89 27.442974644741682 34.0 26.5 35.0 2.0 36.0 90-91 27.300446655643587 34.0 26.0 35.0 2.0 35.5 92-93 27.17014489508074 34.0 26.0 35.0 2.0 35.0 94-95 27.09048812525137 34.0 26.0 35.0 2.0 35.0 96-97 27.014746841576457 34.0 26.0 35.0 2.0 35.0 98-99 26.929647656457515 34.0 25.5 35.0 2.0 35.0 100-101 26.835635317504355 34.0 25.0 35.0 2.0 35.0 102-103 25.6603286889448 31.5 23.5 34.0 2.0 35.0 104-105 26.582248055437418 33.5 25.0 35.0 2.0 35.0 106-107 26.753784308832337 34.0 25.0 35.0 2.0 35.0 108-109 26.761353177554092 34.0 25.0 35.0 2.0 35.0 110-111 26.701989727748686 34.0 25.0 35.0 2.0 35.0 112-113 26.651848231980757 34.0 25.0 35.0 2.0 35.0 114-115 26.5695616392822 34.0 25.0 35.0 2.0 35.0 116-117 26.45975103171905 34.0 25.0 35.0 2.0 35.0 118-119 26.355250155780915 34.0 25.0 35.0 2.0 35.0 120-121 26.224039819660682 34.0 24.5 35.0 2.0 35.0 122-123 26.04010837631849 34.0 24.0 35.0 2.0 35.0 124-125 25.243227233265664 33.0 20.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7337183215811649 1101 2 0.7846871964255726 1101 3 0.6354336182228231 1101 4 0.6379747686515813 1101 5 0.9477648591018806 1101 6 1.02894790734819 1101 7 0.9058952101232194 1101 8 1.0805504141861917 1101 9 1.1180753129582968 1101 10-11 1.173369086894418 1101 12-13 1.1674899827510714 1101 14-15 1.2489099728517417 1101 16-17 1.340832940171822 1101 18-19 1.288763557481289 1101 20-21 1.3727930441214014 1101 22-23 1.1861331518921006 1101 24-25 1.2069107656351648 1101 26-27 1.3093651273759193 1101 28-29 1.4437931877888701 1101 30-31 1.3591006626095563 1101 32-33 1.4511165036533455 1101 34-35 1.4571384092485573 1101 36-37 1.3934364493650122 1101 38-39 1.4885847740441065 1101 40-41 1.49274184944926 1101 42-43 1.536816479445207 1101 44-45 1.527576639947661 1101 46-47 1.520609475699068 1101 48-49 1.5974769326246232 1101 50-51 1.6091205052290292 1101 52-53 1.565352832118986 1101 54-55 1.4827297770449768 1101 56-57 1.494777895940171 1101 58-59 1.3993834050850538 1101 60-61 1.4620797427960603 1101 62-63 1.4498695469660703 1101 64-65 1.5031473190148041 1101 66-67 1.4887268930165405 1101 68-69 1.4915227701464353 1101 70-71 1.4668039586865973 1101 72-73 1.4541547476153696 1101 74-75 1.3704412569954556 1101 76-77 1.3550192695951502 1101 78-79 1.3515366600281702 1101 80-81 1.3360566022489273 1101 82-83 1.3465562066453955 1101 84-85 1.4217802291199 1101 86-87 1.4009682764212457 1101 88-89 1.3718231461221428 1101 90-91 1.3916621953108965 1101 92-93 1.3409553294278354 1101 94-95 1.283987011750881 1101 96-97 1.3670651255790212 1101 98-99 1.3845036525872345 1101 100-101 1.3620538023088322 1101 102-103 1.3416473467787569 1101 104-105 1.3476592983648956 1101 106-107 1.3636294950870607 1101 108-109 1.257819182863578 1101 110-111 1.3107844196560308 1101 112-113 1.2719283106910062 1101 114-115 1.283089308221573 1101 116-117 1.3121923352695788 1101 118-119 1.3773357498375134 1101 120-121 1.2845206365111679 1101 122-123 1.2797244095372307 1101 124-125 1.0962113230754404 1102 1 0.4365075766646527 1102 2 -0.5577718000354253 1102 3 0.1061288491714727 1102 4 0.376808575266395 1102 5 0.7884819830661343 1102 6 0.7164894648675428 1102 7 0.7046294009989467 1102 8 0.6655303521835592 1102 9 0.8920828427743075 1102 10-11 0.942948730922474 1102 12-13 1.0472607068321693 1102 14-15 1.1422930013399935 1102 16-17 1.2698423729463002 1102 18-19 1.1632215380718307 1102 20-21 1.1327211273997548 1102 22-23 1.148600191859984 1102 24-25 1.121641175055423 1102 26-27 1.0950904926971816 1102 28-29 1.201760180632455 1102 30-31 1.0947640468827018 1102 32-33 1.046285363338761 1102 34-35 0.9676833892153311 1102 36-37 0.9380777686728123 1102 38-39 0.8260789554517984 1102 40-41 0.8653915518466988 1102 42-43 0.7428487498797054 1102 44-45 0.6181557364704631 1102 46-47 0.6995254872420062 1102 48-49 0.844465401919372 1102 50-51 0.7745151514765993 1102 52-53 0.8316570133755938 1102 54-55 0.8360299530956148 1102 56-57 0.6305250967206355 1102 58-59 0.85636870158325 1102 60-61 0.7105862621375287 1102 62-63 0.8638210744050099 1102 64-65 0.7479867042836759 1102 66-67 0.7410400487548046 1102 68-69 0.4873772704453714 1102 70-71 0.4371999683013925 1102 72-73 0.53241828542021 1102 74-75 0.6385946534345841 1102 76-77 0.6483050997131201 1102 78-79 0.6594030370902182 1102 80-81 0.5216721067919252 1102 82-83 0.5056495062890498 1102 84-85 0.6066027503636064 1102 86-87 0.5692471458121027 1102 88-89 0.73889987815609 1102 90-91 0.6108712120965016 1102 92-93 0.7841613360137281 1102 94-95 0.6494904395942207 1102 96-97 0.6016732132604403 1102 98-99 0.6825111939145287 1102 100-101 0.46251029650535 1102 102-103 0.4455816864377802 1102 104-105 0.45265576943812746 1102 106-107 0.6716163089158513 1102 108-109 0.7183626160021035 1102 110-111 0.7126006118541213 1102 112-113 0.7195555121333719 1102 114-115 0.7511854360428245 1102 116-117 0.7261845119159283 1102 118-119 0.7278658083076195 1102 120-121 0.7993795405941455 1102 122-123 0.7352086351266607 1102 124-125 0.5616238118069745 1103 1 0.25507307404662427 1103 2 -1.0249388045515815 1103 3 0.01339958392489038 1103 4 0.2809241212491429 1103 5 0.5848165954184594 1103 6 0.605908776645542 1103 7 0.6195580737094986 1103 8 0.6813861557868677 1103 9 0.7456827250248921 1103 10-11 0.8749908525686934 1103 12-13 0.913221654248602 1103 14-15 0.8818729798594731 1103 16-17 0.9627102997842769 1103 18-19 0.9447525135994823 1103 20-21 1.0098177008627118 1103 22-23 0.9894065567403487 1103 24-25 0.8806031731813349 1103 26-27 0.8898248497314754 1103 28-29 0.9642340404086411 1103 30-31 0.8993629046551135 1103 32-33 0.8007027879201161 1103 34-35 0.8819001734613963 1103 36-37 0.921323397153003 1103 38-39 0.8611129031320033 1103 40-41 0.9704212587993055 1103 42-43 1.0066749062794855 1103 44-45 1.0990546832835761 1103 46-47 1.0349532688168424 1103 48-49 1.0041184491954667 1103 50-51 1.0395376739662936 1103 52-53 0.9711937102422645 1103 54-55 1.0072237543468496 1103 56-57 1.0390422556371668 1103 58-59 0.9674514278318966 1103 60-61 0.9345057145444677 1103 62-63 1.0370773848554151 1103 64-65 0.9839292023491168 1103 66-67 0.9786890389224396 1103 68-69 0.9446449448799079 1103 70-71 0.9909902461054827 1103 72-73 0.9268485779138338 1103 74-75 0.8958701243957989 1103 76-77 0.8294130954519865 1103 78-79 0.8721898509047854 1103 80-81 0.9125373463784641 1103 82-83 0.9206277998843824 1103 84-85 0.9876602508408716 1103 86-87 0.9224022617180374 1103 88-89 0.983479470492469 1103 90-91 0.9488951641923116 1103 92-93 0.9626170553853974 1103 94-95 0.891316765040191 1103 96-97 0.8811559610770487 1103 98-99 0.849923679724121 1103 100-101 0.8752780197592571 1103 102-103 0.9049986519713045 1103 104-105 0.8709818332996946 1103 106-107 0.8246196475780252 1103 108-109 0.8281185482845359 1103 110-111 0.8506416142980022 1103 112-113 0.8589020320407421 1103 114-115 0.8256815399654265 1103 116-117 0.796981637547848 1103 118-119 0.8046374779155947 1103 120-121 0.7986412022307192 1103 122-123 0.8370462108877668 1103 124-125 0.7663351727307841 1104 1 0.3260906746196852 1104 2 -0.7313944334747475 1104 3 0.10023807008274943 1104 4 0.3395403892664248 1104 5 0.679115809752382 1104 6 0.5739565388059162 1104 7 0.7817550688008055 1104 8 0.7904811244933896 1104 9 0.8289794000640427 1104 10-11 0.9307347155569801 1104 12-13 0.7906088151976434 1104 14-15 0.8638660277938328 1104 16-17 1.0243724300827068 1104 18-19 0.9582309801042115 1104 20-21 0.9960242045338958 1104 22-23 0.9477042075482842 1104 24-25 0.9283947501155438 1104 26-27 0.8061029690921018 1104 28-29 1.0358178738713093 1104 30-31 1.0142658787983905 1104 32-33 1.07401215369336 1104 34-35 1.0949588584924115 1104 36-37 1.1258730339374239 1104 38-39 1.1488928626777763 1104 40-41 1.2076807604913995 1104 42-43 1.1964270380473039 1104 44-45 1.1090572891602335 1104 46-47 1.0887839648958604 1104 48-49 1.1727057540991055 1104 50-51 1.192470547961257 1104 52-53 0.9329630603113763 1104 54-55 1.1099086108743954 1104 56-57 1.2149732825591961 1104 58-59 1.1622147924204427 1104 60-61 1.0989323126241537 1104 62-63 1.0730584285377134 1104 64-65 1.0945696166261065 1104 66-67 1.0680579805462074 1104 68-69 1.039133831447355 1104 70-71 1.0560251212300358 1104 72-73 1.0572688874506504 1104 74-75 1.1152680922711973 1104 76-77 1.0452286717065995 1104 78-79 1.0975690231773392 1104 80-81 0.953983326670226 1104 82-83 0.9820137589158726 1104 84-85 0.9765487411313991 1104 86-87 0.981449762142784 1104 88-89 0.9594664606245793 1104 90-91 0.9542859997718232 1104 92-93 0.9912482067466577 1104 94-95 0.942740899590671 1104 96-97 1.0009845299801015 1104 98-99 1.0741636706322524 1104 100-101 1.0474168647704367 1104 102-103 1.041873872954234 1104 104-105 1.0008581517355282 1104 106-107 1.0519956445841352 1104 108-109 0.9900898824933826 1104 110-111 0.8857903845997335 1104 112-113 0.9184755292420093 1104 114-115 0.9580795319480515 1104 116-117 0.9771258906140581 1104 118-119 0.9375937244430048 1104 120-121 0.8894172885483727 1104 122-123 0.9228338227567896 1104 124-125 0.9270124079300928 1105 1 0.5690109478131227 1105 2 0.36812539687794654 1105 3 0.5895086207092568 1105 4 0.4722645715893137 1105 5 0.6554264605209354 1105 6 0.5045729338161422 1105 7 0.5970032886181613 1105 8 0.6439495020746904 1105 9 0.6129297499113235 1105 10-11 0.788830233457837 1105 12-13 0.8075465645083142 1105 14-15 0.7759245409506264 1105 16-17 0.9130534741161114 1105 18-19 0.9462779936369969 1105 20-21 0.9600121579350471 1105 22-23 0.8357834438568901 1105 24-25 0.9377110199482175 1105 26-27 0.9657964156061638 1105 28-29 1.050169774434849 1105 30-31 0.9906001260774389 1105 32-33 0.9850711998991954 1105 34-35 1.0567405381616624 1105 36-37 1.0057226003805582 1105 38-39 1.0541361427010543 1105 40-41 0.9861928883241404 1105 42-43 1.050053628297377 1105 44-45 0.9599530668548013 1105 46-47 0.9924183303895617 1105 48-49 1.1116668036099995 1105 50-51 1.1067074832309984 1105 52-53 1.0042222977649935 1105 54-55 1.0310158055664935 1105 56-57 0.957990552883917 1105 58-59 0.9267574959152043 1105 60-61 1.0385154783320054 1105 62-63 1.0755164306172986 1105 64-65 1.022660024528541 1105 66-67 0.9834470831360989 1105 68-69 0.8964645903111332 1105 70-71 0.9133668079859731 1105 72-73 0.88166916859727 1105 74-75 0.9082759239917166 1105 76-77 0.9902384545877574 1105 78-79 1.0351403929509075 1105 80-81 0.7524183558061139 1105 82-83 0.8166679810665762 1105 84-85 0.6771226585544419 1105 86-87 0.7483396414833834 1105 88-89 0.846161916323485 1105 90-91 0.8464267370970191 1105 92-93 0.7217426928997561 1105 94-95 0.7686846246209207 1105 96-97 0.8152048509854808 1105 98-99 0.8593833966961064 1105 100-101 0.8851543965290212 1105 102-103 0.6154288381242381 1105 104-105 0.4392337370063544 1105 106-107 0.7615665052920022 1105 108-109 0.8085208267251573 1105 110-111 0.7334552777887744 1105 112-113 0.5828288997520978 1105 114-115 0.7390097218651448 1105 116-117 0.42421593799275215 1105 118-119 0.3970160431831218 1105 120-121 0.3935394248603856 1105 122-123 0.4652051289358283 1105 124-125 0.3899704316720545 1106 1 0.39448213086904005 1106 2 0.4639948723619618 1106 3 0.5701077842456463 1106 4 0.40543332042909697 1106 5 0.5811280520811906 1106 6 0.5882057092357158 1106 7 0.5190436736973041 1106 8 0.5398806867347332 1106 9 0.6231773433001635 1106 10-11 0.7459669650574838 1106 12-13 0.6786340406016791 1106 14-15 0.8610096306177724 1106 16-17 0.9551339541451682 1106 18-19 0.845164893021817 1106 20-21 0.888894054125636 1106 22-23 0.8660778716277306 1106 24-25 0.9008573097974448 1106 26-27 0.9122732607192034 1106 28-29 1.1069934894420186 1106 30-31 0.9508166980606418 1106 32-33 0.9547741163917607 1106 34-35 0.9798770885306425 1106 36-37 0.9751521494938657 1106 38-39 0.9753533695435337 1106 40-41 1.0789460771097836 1106 42-43 1.068384056112528 1106 44-45 0.9577849982305722 1106 46-47 0.8773176652943633 1106 48-49 0.8845729121111177 1106 50-51 0.9450092904797742 1106 52-53 0.9537017533736893 1106 54-55 0.9392669523042194 1106 56-57 0.8068389574334738 1106 58-59 0.788942702715012 1106 60-61 0.9419753959934916 1106 62-63 0.9789837282905403 1106 64-65 0.8940875283243059 1106 66-67 0.7864419799847546 1106 68-69 0.8963984237681579 1106 70-71 0.890167774407189 1106 72-73 1.0417217521819104 1106 74-75 0.9533194987573061 1106 76-77 0.9816244194602355 1106 78-79 0.7397452934121667 1106 80-81 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 159092.0 3 50347.0 4 23160.0 5 18493.0 6 20290.0 7 24524.0 8 27202.0 9 29261.0 10 31764.0 11 36035.0 12 40941.0 13 46430.0 14 50334.0 15 40645.0 16 25651.0 17 21752.0 18 20059.0 19 18336.0 20 18042.0 21 18991.0 22 21826.0 23 28448.0 24 39374.0 25 52263.0 26 62455.0 27 69109.0 28 79645.0 29 92625.0 30 108593.0 31 127871.0 32 155483.0 33 192943.0 34 249886.0 35 343778.0 36 542567.0 37 801163.0 38 292887.0 39 893.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.186680024508355 20.008827630836215 13.488916349279759 24.315575995375667 2 15.566559792848542 20.90928757013359 38.049658763397645 25.474493873620226 3 18.763654366711037 25.47722184256813 29.600030930231743 26.159092860489093 4 12.95933811322498 16.96679920337371 36.00365170598745 34.07021097741386 5 13.211721764060899 39.05465739863149 33.129885596121206 14.6037352411864 6 31.96693262929934 37.21414833168095 16.54884926242882 14.270069776590885 7 28.05222395423927 33.71472298453009 20.232664926504203 18.000388134726432 8 26.724304364603253 35.761809037404255 18.82388768506991 18.68999891292259 9 26.896914634385155 15.904091409611446 19.606710909973724 37.59228304602968 10-11 25.021456375762213 27.35106675209442 26.302646623382547 21.32483024876082 12-13 25.95250655258223 24.579508639817256 27.64986324882036 21.81812155878016 14-15 22.87951361798097 25.540190477864304 26.173626981999824 25.4066689221549 16-17 22.044521787532965 28.73018735743601 26.659557253799004 22.56573360123202 18-19 21.939986221966926 26.62042523107328 29.862016312709134 21.577572234250663 20-21 22.762224079958955 25.77767059314766 30.060635338429403 21.39946998846398 22-23 22.515801944856683 25.692122485520663 29.898119560087817 21.893956009534836 24-25 22.102788792209598 25.898734120012314 30.02950171697934 21.96897537079875 26-27 21.909525055245112 26.166097352904405 30.01156368891216 21.91281390293832 28-29 21.85298612513602 25.98527711044327 30.158788770292578 22.002947994128128 30-31 21.978515539679822 25.85943113479305 30.085274046372252 22.07677927915488 32-33 21.8375036483064 25.909672640997233 30.041281567657645 22.211542143038717 34-35 22.187018441146446 25.92062629702362 29.90742521386297 21.984930047966966 36-37 22.274995920314325 25.775152831301863 29.63713392666608 22.312717321717738 38-39 22.20559666475696 25.94260634401146 29.73679427228345 22.115002718948134 40-41 22.371055830574633 25.892884490145757 29.509524854399448 22.226534824880158 42-43 22.31414731584515 25.967147738766137 29.197553560890515 22.521151384498197 44-45 22.352377184133797 26.104136466592585 29.0397594069831 22.503726942290513 46-47 22.48659541825283 26.442431410493146 28.37733072267121 22.693642448582818 48-49 22.68403316723772 26.536526704070084 28.033106983861384 22.746333144830817 50-51 22.69108378199584 26.765864074403705 27.79147580988342 22.751576333717036 52-53 22.983626288018563 26.7099205076983 27.59050430651296 22.715948897770183 54-55 22.679140521164364 26.748047654649902 27.660803312346637 22.912008511839097 56-57 22.672534984516393 26.789232512951333 27.633980055903923 22.904252446628348 58-59 22.78061095758967 26.433981889240115 27.73659185414986 23.04881529902035 60-61 22.748984599656854 26.546335847084144 27.917935969399153 22.786743583859842 62-63 22.77693980504916 26.57253365294573 27.976570349456388 22.67395619254873 64-65 22.522824697809927 26.48563736488172 27.996408854793987 22.995129082514367 66-67 22.62714660076251 26.701790388430485 27.818078017492653 22.85298499331435 68-69 22.626442805586066 26.462199905852817 28.051391809195042 22.859965479366075 70-71 22.44025971352379 26.429016875554524 27.914709885974897 23.21601352494679 72-73 22.75219782758025 26.711664303303216 27.544059488469063 22.992078380647467 74-75 22.611478481111817 26.81255627087954 27.606637867358145 22.9693273806505 76-77 22.691023865751962 26.582067464706498 27.75495027848043 22.971958391061108 78-79 22.658158250514987 26.617760220330066 27.722084348556344 23.0019971805986 80-81 22.503132307855306 26.943031978600512 27.56039256501022 22.99344314853397 82-83 22.621505592307596 26.800798363104576 27.463351869751328 23.1143441748365 84-85 22.608592718604854 26.6143655344267 27.41629335464644 23.36074839232201 86-87 22.49673090540583 26.774766569009124 27.520309685595763 23.208192839989287 88-89 22.649016515164163 26.646849892327886 27.452103011241707 23.252030581266244 90-91 22.621234206627 26.744407327050546 27.503967957083297 23.130390509239152 92-93 22.572358351477952 26.70432176630806 27.444915858528233 23.278404023685752 94-95 22.39766068656886 26.566543087686846 27.548998488028005 23.486797737716294 96-97 22.603846088663285 26.512377043983903 27.453832059861245 23.42994480749157 98-99 22.324117715509594 26.558834720042356 27.214980047633613 23.90206751681443 100-101 22.2396359052039 26.722244280483842 27.379565134378332 23.65855467993393 102-103 22.377782843554208 26.491116591968783 27.443502087289577 23.687598477187436 104-105 22.293733453674054 26.41416873894558 27.557991381160818 23.734106426219547 106-107 22.318629243552408 26.49619709262468 27.41481511331016 23.770358550512753 108-109 22.262533552905943 26.472455044566896 27.204649690212307 24.060361712314855 110-111 22.15235231005671 26.67329715312118 27.265270799887475 23.909079736934636 112-113 22.207035435273635 26.613054574893336 27.30611352840181 23.87379646143122 114-115 22.095142071401995 26.72365356158888 27.177592139664757 24.00361222734437 116-117 21.909334132720033 26.615246317931707 27.365763477693683 24.109656071654573 118-119 21.814465146687102 26.716780867659747 27.21341208063397 24.255341905019186 120-121 21.877511726727146 26.84817262425334 27.192184240875676 24.08213140814384 122-123 21.84998424599842 26.82265011178437 27.029950177185448 24.297415465031765 124-125 21.70762052135276 26.765012657916404 27.060324289202793 24.467042531528048 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 297.0 1 252.5 2 464.5 3 881.5 4 752.5 5 692.5 6 1813.5 7 4005.5 8 6147.5 9 6831.0 10 5488.5 11 3994.5 12 3840.5 13 4172.0 14 4486.0 15 4923.5 16 5449.0 17 5936.0 18 6462.5 19 7005.0 20 7685.5 21 8608.5 22 9941.0 23 11702.0 24 13952.0 25 17099.5 26 21150.5 27 26289.0 28 31516.5 29 36967.5 30 44351.0 31 52247.0 32 61173.5 33 68270.0 34 73752.5 35 80727.0 36 88826.5 37 97472.0 38 103112.0 39 107744.0 40 113120.0 41 118168.0 42 121774.0 43 128422.5 44 137812.0 45 142959.5 46 142914.5 47 144301.5 48 150187.5 49 149781.0 50 144679.0 51 139466.0 52 133927.5 53 132361.0 54 132175.0 55 126295.0 56 116407.0 57 107715.0 58 101844.0 59 93845.0 60 79998.5 61 65260.0 62 53226.5 63 42116.5 64 31547.0 65 23809.5 66 18030.5 67 13791.0 68 10256.0 69 7472.5 70 5510.5 71 4059.0 72 3272.5 73 2990.0 74 2341.0 75 1666.5 76 1217.5 77 708.0 78 431.5 79 298.5 80 206.5 81 139.0 82 86.5 83 65.0 84 54.0 85 34.0 86 23.5 87 18.0 88 13.5 89 10.0 90 4.0 91 4.5 92 3.5 93 1.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06213662626488831 2 0.07720005081395215 3 0.0 4 0.009088266144601845 5 0.03263741985630497 6 4.2679702889014193E-4 7 1.2552853790886526E-4 8 2.5105707581773052E-5 9 8.033826426167377E-4 10-11 0.060416885295536855 12-13 0.009464851758328443 14-15 0.007042150976687342 16-17 0.05149180625021654 18-19 1.5063424549063834E-4 20-21 0.010406315792644932 22-23 0.012540300937095641 24-25 0.0 26-27 0.0 28-29 0.053876848470484975 30-31 0.0 32-33 0.0036528804531479795 34-35 0.0 36-37 2.0084566065418441E-4 38-39 0.0 40-41 0.0 42-43 0.0036277747455662067 44-45 0.0 46-47 1.2552853790886526E-4 48-49 2.6360992960861707E-4 50-51 5.774312743807802E-4 52-53 1.2552853790886526E-4 54-55 0.0 56-57 0.0018703752148420925 58-59 2.5105707581773052E-5 60-61 0.0 62-63 0.0 64-65 6.90406958498759E-4 66-67 0.0 68-69 8.284883501985109E-4 70-71 0.0 72-73 0.0033641648159575895 74-75 0.004820295855700427 76-77 5.899841281716667E-4 78-79 0.0010795454260162413 80-81 0.0018201637996785465 82-83 2.0084566065418441E-4 84-85 1.6318709928152486E-4 86-87 5.397727130081206E-4 88-89 1.2552853790886526E-5 90-91 0.0 92-93 0.008586151992966385 94-95 0.007029598122896455 96-97 0.007042150976687342 98-99 0.001167415402552447 100-101 4.895612978445745E-4 102-103 5.0211415163546103E-5 104-105 5.774312743807802E-4 106-107 0.005485597106617413 108-109 0.006753435339496952 110-111 0.0010418868646435819 112-113 0.004104783189619894 114-115 5.02114151635461E-4 116-117 0.002623546442295284 118-119 0.0030628963249763126 120-121 0.014787261765664328 122-123 0.0010795454260162413 124-125 0.001945692337587412 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3983158.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.441560283381634 #Duplication Level Percentage of deduplicated Percentage of total 1 70.20926738627199 26.98953784681435 2 11.729040416062698 9.017652284405878 3 5.396181469458792 6.223129057748012 4 3.1949402145465204 4.912739474371612 5 2.0940426020363465 4.024913246107477 6 1.417672960282308 3.2698536338887467 7 1.021815652055419 2.749613159089391 8 0.775247590012087 2.384138157279678 9 0.5917026721986413 2.0471376548845863 >10 3.2329241301388087 22.14259725881092 >50 0.22191052496005081 5.8728365730788035 >100 0.10936609545248654 7.433385509852268 >500 0.004366594511873567 1.1404981733911488 >1k 0.0013232104452904415 0.7589502427125228 >5k 6.616052226452202E-5 0.25010946975084436 >10k+ 1.3232104452904405E-4 0.7829082578137321 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16781 0.42129887892973367 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14002 0.35153011755998637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9834 0.24688952835915623 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4215 0.10582055745717343 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.531712274531917E-5 0.0 0.0 0.0 0.0 3 7.531712274531917E-5 0.0 0.0 0.0 0.0 4 7.531712274531917E-5 0.0 0.0 0.0 0.0 5 1.0042283032709222E-4 0.0 0.0 0.0 0.0 6 1.2552853790886529E-4 0.0 0.0 0.0 0.0 7 2.761627833995036E-4 0.0 0.0 0.0 0.0 8 3.012684909812767E-4 0.0 0.0 0.0 0.0 9 3.012684909812767E-4 0.0 0.0 0.0 0.0 10-11 3.012684909812767E-4 0.0 0.0 2.5105707581773055E-5 0.0 12-13 3.389270523539362E-4 0.0 0.0 7.531712274531917E-5 0.0 14-15 4.2679702889014193E-4 0.0 0.0 1.0042283032709223E-4 0.0 16-17 5.272198592172341E-4 0.0 0.0 1.6318709928152486E-4 0.0 18-19 7.280655198714187E-4 0.0 0.0 2.0084566065418444E-4 0.0 20-21 8.535940577802839E-4 0.0 0.0 2.3850422202684402E-4 0.0 22-23 9.289111805256031E-4 0.0 0.0 3.514799061448228E-4 0.0 24-25 0.0011548625487615605 0.0 0.0 8.033826426167379E-4 0.0 26-27 0.0011548625487615605 0.0 0.0 0.001242732525297766 0.0 28-29 0.0015565538700699293 0.0 0.0 0.0024101479278502134 1.2552853790886528E-5 30-31 0.0017071881155605677 0.0 0.0 0.005686442767271597 2.5105707581773055E-5 32-33 0.0017197409693514541 0.0 0.0 0.011285015558006989 2.5105707581773055E-5 34-35 0.0019331394837965254 0.0 0.0 0.01836482509606699 2.5105707581773055E-5 36-37 0.002334830805104894 0.0 0.0 0.030704280372508445 2.5105707581773055E-5 38-39 0.0025105707581773054 0.0 0.0 0.053412392880222176 2.5105707581773055E-5 40-41 0.0025356764657590786 0.0 0.0 0.07963530444938413 2.5105707581773055E-5 42-43 0.002610993588504398 0.0 0.0 0.10265723830187003 2.5105707581773055E-5 44-45 0.0027616278339950362 0.0 0.0 0.12949523970678542 2.5105707581773055E-5 46-47 0.0031131077401398586 0.0 0.0 0.16271009083747118 2.5105707581773055E-5 48-49 0.0032386362780487243 0.0 0.0 0.19938952961444162 2.5105707581773055E-5 50-51 0.0032386362780487243 0.0 0.0 0.2410398984926031 2.5105707581773055E-5 52-53 0.0032386362780487243 2.5105707581773055E-5 0.0 0.28151029911442127 2.5105707581773055E-5 54-55 0.0032888476932122703 2.5105707581773055E-5 0.0 0.3256335801893874 2.5105707581773055E-5 56-57 0.003640327599357093 2.5105707581773055E-5 0.0 0.37747686634574873 2.5105707581773055E-5 58-59 0.003640327599357093 2.5105707581773055E-5 0.0 0.4370150518759236 2.5105707581773055E-5 60-61 0.0036779861607297527 2.5105707581773055E-5 0.0 0.5044615352943569 2.5105707581773055E-5 62-63 0.0037156447221024123 2.5105707581773055E-5 0.0 0.5722218400575623 2.5105707581773055E-5 64-65 0.0037658561372659583 2.5105707581773055E-5 0.0 0.6419278371583552 2.5105707581773055E-5 66-67 0.004092230335829008 2.5105707581773055E-5 0.0 0.7242620051727799 2.5105707581773055E-5 68-69 0.004142441750992554 2.5105707581773055E-5 0.0 0.8195130597380269 2.5105707581773055E-5 70-71 0.004293075996483193 2.5105707581773055E-5 0.0 0.929250107577957 2.5105707581773055E-5 72-73 0.004393498826810284 2.5105707581773055E-5 0.0 1.0439455326652873 2.5105707581773055E-5 74-75 0.004456263095764718 2.5105707581773055E-5 0.0 1.1583145835540543 2.5105707581773055E-5 76-77 0.0044688159495556035 2.5105707581773055E-5 0.0 1.2881989617283574 2.5105707581773055E-5 78-79 0.0044688159495556035 2.5105707581773055E-5 0.0 1.4375277104247433 2.5105707581773055E-5 80-81 0.005360068568708547 2.5105707581773055E-5 0.0 1.6093637259681892 2.5105707581773055E-5 82-83 0.005447938545244753 2.5105707581773055E-5 0.0 1.794003652378339 2.5105707581773055E-5 84-85 0.005636231352108051 2.5105707581773055E-5 0.0 1.9851082984907955 2.5105707581773055E-5 86-87 0.005887288427925781 2.5105707581773055E-5 0.0 2.184083583930138 2.5105707581773055E-5 88-89 0.005950052696880214 2.5105707581773055E-5 0.0 2.411202367568648 2.5105707581773055E-5 90-91 0.0064898254098883345 2.5105707581773055E-5 0.0 2.6708330425255538 2.5105707581773055E-5 92-93 0.006703223924333406 2.5105707581773055E-5 0.0 2.9525190815930475 2.5105707581773055E-5 94-95 0.006703223924333406 2.5105707581773055E-5 0.0 3.2382471395812065 2.5105707581773055E-5 96-97 0.006703223924333406 2.5105707581773055E-5 0.0 3.529649087482847 2.5105707581773055E-5 98-99 0.006954281000151136 2.5105707581773055E-5 0.0 3.8518431857335313 2.5105707581773055E-5 100-101 0.007330866613877732 2.5105707581773055E-5 0.0 4.228704962243526 2.5105707581773055E-5 102-103 0.007707452227604328 2.5105707581773055E-5 0.0 4.635881880658513 2.5105707581773055E-5 104-105 0.008623810554339044 2.5105707581773055E-5 0.0 5.045017044264878 2.5105707581773055E-5 106-107 0.009025501875647414 5.021141516354611E-5 0.0 5.454315394970523 2.5105707581773055E-5 108-109 0.009151030413556279 5.021141516354611E-5 0.0 5.89435568461005 2.5105707581773055E-5 110-111 0.00932677036662869 5.021141516354611E-5 0.0 6.390042775104578 2.5105707581773055E-5 112-113 0.00940208748937401 5.021141516354611E-5 0.0 6.927480657307594 2.5105707581773055E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11225 0.0 57.59424 1 GTATCAA 19580 0.0 51.85523 1 ATCAACG 25900 0.0 39.056686 3 TATCAAC 25770 0.0 39.050274 2 TCAACGC 26040 0.0 38.801975 4 CAACGCA 26225 0.0 37.95709 5 AACGCAG 26825 0.0 37.0941 6 GTACATG 22175 0.0 32.217453 1 ACGCAGA 31590 0.0 31.272667 7 TACATGG 22750 0.0 30.937687 2 CGCAGAG 31800 0.0 30.841438 8 ACATGGG 24430 0.0 27.937414 3 GCAGAGT 34410 0.0 27.567617 9 GAGTACT 19645 0.0 25.613087 12-13 AGTACTT 20325 0.0 24.49249 12-13 CATGGGA 15725 0.0 24.407663 4 GTACTTT 20850 0.0 24.018572 14-15 AGAGTAC 32630 0.0 23.106653 10-11 CATGGGG 9135 0.0 23.059605 4 CAGAGTA 34130 0.0 22.806839 10-11 >>END_MODULE