FastQCFastQC Report
Thu 26 May 2016
SRR1364628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522574
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111510.7323781963963656No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA47640.31289119609293214No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA43840.2879334600485757No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA43560.28609446897162305No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT41350.27157957511424735No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT25000.16419563187076622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.13227600103508924No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG18960.1245259672107891No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17930.11776110717771353No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG17690.11618482911175418No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA16860.11073353413364473No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15720.1032462133203378No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT15320.10061908321040552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC9950.032.3336941
ACGCATA3100.028.79957
CGCATAG3200.027.8949248
GTTATCA12850.027.35481
TAGCCCT14900.027.1898784
TATAACG1159.973258E-425.90342
AACGCAT3450.025.8803716
CGCAGAT5100.025.6706228
TACCTGG17200.025.6323182
CATGGGG33450.025.1135234
GTACATG186250.024.8867381
TACATGG183650.024.4604852
ATAGCCC9750.024.4421843
CGATCGT751.6750259E-423.82602524-25
TCGCGGA751.6900552E-423.79465782-83
TTATCAA14800.022.9455152
GTACCTG22000.022.4770891
ACATGGG189500.022.3220373
GTGTAGC12650.022.1356351
ATGGGAG29450.021.6484155
Produced by FastQC (version 0.11.5)