##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364628_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1522574 Sequences flagged as poor quality 0 Sequence length 125 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.809567876503866 31.0 25.0 31.0 7.0 34.0 2 25.33039576401541 30.0 23.0 31.0 7.0 34.0 3 26.006236150098452 31.0 25.0 31.0 7.0 34.0 4 29.13592114406262 35.0 28.0 37.0 7.0 37.0 5 29.573086102875788 35.0 30.0 37.0 7.0 37.0 6 29.630419933612423 35.0 30.0 37.0 2.0 37.0 7 29.42939325116546 35.0 29.0 37.0 2.0 37.0 8 29.183876120306795 35.0 28.0 37.0 2.0 37.0 9 29.978473952661744 37.0 27.0 39.0 2.0 39.0 10-11 29.999253566657515 36.0 27.5 39.0 2.0 39.0 12-13 29.987162528717818 37.0 27.0 39.0 2.0 39.0 14-15 30.54931221733722 37.0 27.0 40.0 2.0 41.0 16-17 30.950419487000303 37.0 28.0 40.0 2.0 41.0 18-19 30.81253719031062 37.0 27.5 40.0 2.0 41.0 20-21 30.759850752738455 37.0 27.0 40.0 2.0 41.0 22-23 30.843281180422103 37.0 27.0 40.0 2.0 41.0 24-25 30.974857051282893 37.0 28.0 40.0 2.0 41.0 26-27 30.801946572054952 37.0 27.5 40.0 2.0 41.0 28-29 30.708611207074334 37.0 28.0 40.0 2.0 41.0 30-31 30.57954949972875 37.0 27.5 40.0 2.0 41.0 32-33 30.453764480412776 37.0 27.0 40.0 2.0 41.0 34-35 30.482193968897406 37.0 28.0 40.0 2.0 41.0 36-37 30.383204363137686 37.0 28.0 40.0 2.0 41.0 38-39 30.06456008049527 36.0 27.0 40.0 2.0 41.0 40-41 29.920350997718337 36.0 27.0 40.0 2.0 41.0 42-43 29.403861815583348 35.5 25.0 40.0 2.0 41.0 44-45 29.25225834672075 35.0 25.0 40.0 2.0 41.0 46-47 29.167486112333457 35.0 25.0 40.0 2.0 41.0 48-49 29.062951291694198 35.0 25.0 40.0 2.0 41.0 50-51 28.827915753191633 35.0 24.0 40.0 2.0 41.0 52-53 28.729664370992808 35.0 24.0 40.0 2.0 41.0 54-55 28.567590475077075 35.0 24.0 39.0 2.0 41.0 56-57 28.358602603223225 35.0 23.5 39.0 2.0 41.0 58-59 28.15288616513877 34.0 23.0 39.0 2.0 40.0 60-61 27.851484394190365 34.0 22.0 39.0 2.0 40.0 62-63 27.647802668376052 34.0 22.0 39.0 2.0 40.0 64-65 27.41513680123265 34.0 22.0 38.0 2.0 40.0 66-67 27.154243406231814 33.5 21.0 38.0 2.0 40.0 68-69 26.922117414326003 33.0 20.5 37.5 2.0 40.0 70-71 26.459149111964344 33.0 18.5 37.0 2.0 39.5 72-73 26.169231183508977 32.5 18.0 36.5 2.0 39.0 74-75 26.11519275910399 33.0 18.0 36.0 2.0 39.0 76-77 25.81726635289976 32.5 15.5 36.0 2.0 39.0 78-79 25.510935429082593 32.0 12.5 35.5 2.0 38.5 80-81 25.10691959799655 32.0 8.0 35.0 2.0 37.0 82-83 25.165221854569957 32.0 9.0 35.0 2.0 37.0 84-85 24.96664661290683 32.0 7.0 35.0 2.0 36.5 86-87 24.787767622460386 32.0 6.0 35.0 2.0 36.0 88-89 24.57099983317724 32.0 2.0 35.0 2.0 36.0 90-91 24.390754078291103 32.0 2.0 35.0 2.0 35.5 92-93 24.244847212680632 32.0 2.0 35.0 2.0 35.0 94-95 24.150024235275264 32.0 2.0 35.0 2.0 35.0 96-97 24.00859531293717 32.0 2.0 35.0 2.0 35.0 98-99 23.88239619223762 32.0 2.0 35.0 2.0 35.0 100-101 23.73860646510449 32.0 2.0 35.0 2.0 35.0 102-103 22.616278420621917 29.5 2.0 34.0 2.0 35.0 104-105 23.394323034545447 31.0 2.0 34.5 2.0 35.0 106-107 23.52510649728683 32.0 2.0 35.0 2.0 35.0 108-109 23.47987224266275 32.0 2.0 35.0 2.0 35.0 110-111 23.40007218039977 32.0 2.0 35.0 2.0 35.0 112-113 23.279568349387286 32.0 2.0 35.0 2.0 35.0 114-115 23.124082967396 32.0 2.0 35.0 2.0 35.0 116-117 22.98286946972692 32.0 2.0 35.0 2.0 35.0 118-119 22.862911424994778 32.0 2.0 35.0 2.0 35.0 120-121 22.659592899918167 31.5 2.0 35.0 2.0 35.0 122-123 22.394062948664562 31.0 2.0 35.0 2.0 35.0 124-125 21.46203107369494 30.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.306165520776979 1101 2 1.5528287586962541 1101 3 1.1996186176414376 1101 4 1.2209675236529662 1101 5 0.9673441245072247 1101 6 0.912532985207374 1101 7 1.0169365158502046 1101 8 1.124637714858789 1101 9 1.4482906418654196 1101 10-11 1.4398064884556305 1101 12-13 1.4602196284997007 1101 14-15 1.6491495960253602 1101 16-17 1.1683195017583863 1101 18-19 1.168495912285831 1101 20-21 1.4204875313094938 1101 22-23 1.2963429489195768 1101 24-25 1.3968064159334617 1101 26-27 1.6478680557176943 1101 28-29 1.6594730228225139 1101 30-31 1.7444019340066745 1101 32-33 1.7988624159936393 1101 34-35 1.6103878964985832 1101 36-37 1.4076102190206292 1101 38-39 1.3774574725701036 1101 40-41 1.5600191354954767 1101 42-43 1.6485169635010735 1101 44-45 1.3767753775307412 1101 46-47 1.0494097477048427 1101 48-49 0.9303174214364631 1101 50-51 0.8945252047359276 1101 52-53 0.8041005424374639 1101 54-55 0.8232482016339269 1101 56-57 0.8891906037385695 1101 58-59 0.680605243464143 1101 60-61 0.8395803617487019 1101 62-63 0.6865814719344385 1101 64-65 0.5840951198045694 1101 66-67 0.7799974143483297 1101 68-69 0.44168552375941417 1101 70-71 0.4492575673182664 1101 72-73 0.41275136417433345 1101 74-75 0.4589560450223722 1101 76-77 0.5187589841287057 1101 78-79 0.42275756714979806 1101 80-81 0.4810991903608226 1101 82-83 0.4743125410168645 1101 84-85 0.4661489304310642 1101 86-87 0.4069300322869509 1101 88-89 0.33840397097609554 1101 90-91 0.48509405975099185 1101 92-93 0.4461396057670548 1101 94-95 0.33529432392475655 1101 96-97 0.4507712031519553 1101 98-99 0.549423259211764 1101 100-101 0.5635361431112997 1101 102-103 0.3241851877797899 1101 104-105 0.4512271763813125 1101 106-107 0.5443594399820171 1101 108-109 0.5216033301112404 1101 110-111 0.6593440737297414 1101 112-113 0.6122118426828216 1101 114-115 0.6440608358975801 1101 116-117 0.6018229075722026 1101 118-119 0.4999034543579164 1101 120-121 0.5454949997186418 1101 122-123 0.4990615702411745 1101 124-125 0.48024701152872495 1102 1 0.8429947003836809 1102 2 1.0640202751988994 1102 3 0.69688059549485 1102 4 0.6713161346730097 1102 5 0.4248131826713184 1102 6 0.15695133432705433 1102 7 0.5441144654332426 1102 8 0.3231492878703932 1102 9 0.8106865503304377 1102 10-11 0.7260807119044621 1102 12-13 0.8636949098026641 1102 14-15 1.0398918821745013 1102 16-17 0.6946637255847463 1102 18-19 0.5857174357626818 1102 20-21 0.8640945079011964 1102 22-23 0.7546676886783175 1102 24-25 0.45147853877487876 1102 26-27 0.8541054013065619 1102 28-29 0.416804881836228 1102 30-31 0.6191558001217672 1102 32-33 0.7472871591302024 1102 34-35 0.7230609849994885 1102 36-37 0.5638830317323631 1102 38-39 0.8456907528517732 1102 40-41 0.7483926748361682 1102 42-43 0.9653866968320237 1102 44-45 0.5470527363924589 1102 46-47 0.4096727607611115 1102 48-49 0.27652283667997324 1102 50-51 0.40708851404698976 1102 52-53 0.355784187623172 1102 54-55 0.28580058269984576 1102 56-57 0.3787503544156259 1102 58-59 0.32747400318779896 1102 60-61 0.31542526411973526 1102 62-63 -0.045501868849971316 1102 64-65 0.016833161820688503 1102 66-67 0.10990176239902638 1102 68-69 0.11170587016491851 1102 70-71 0.15493101545778032 1102 72-73 -0.16638005890174767 1102 74-75 0.040653022667154204 1102 76-77 -0.15696908536698473 1102 78-79 -0.0043522299226239625 1102 80-81 0.07696970456456143 1102 82-83 0.02083606754170475 1102 84-85 0.09250189685353405 1102 86-87 -0.00844233276412254 1102 88-89 -0.06884970141101121 1102 90-91 0.0013100853270628932 1102 92-93 -0.030997114527490766 1102 94-95 -0.17619761997162087 1102 96-97 -0.22716466990555162 1102 98-99 -0.01889238029920648 1102 100-101 -0.09470981393473821 1102 102-103 -0.13609262808126488 1102 104-105 -0.02130130163205024 1102 106-107 -0.23910662185595655 1102 108-109 -0.1455088115665042 1102 110-111 -0.08688786734516185 1102 112-113 -0.22106486247113466 1102 114-115 -0.12445031985375365 1102 116-117 -0.07115571479334548 1102 118-119 -0.06953282701856622 1102 120-121 -0.0560478698000999 1102 122-123 -0.19878576416113702 1102 124-125 -0.4255553899034723 1103 1 1.1262369292851915 1103 2 1.361137743832515 1103 3 0.9973514602463887 1103 4 0.9297376042795236 1103 5 0.8064151382319835 1103 6 0.8535733795661216 1103 7 0.8558382263532778 1103 8 0.9424243985909122 1103 9 1.55285169382336 1103 10-11 1.5640758253642169 1103 12-13 1.3715588903415146 1103 14-15 1.5830169970471673 1103 16-17 1.3636163663669656 1103 18-19 1.279863938301002 1103 20-21 1.691927892463955 1103 22-23 1.5490580262691402 1103 24-25 1.4746570372106405 1103 26-27 1.7624773600590657 1103 28-29 1.6277170858681735 1103 30-31 1.6783514770134573 1103 32-33 1.7680940168164057 1103 34-35 1.5762728147089113 1103 36-37 1.3629875267905263 1103 38-39 1.3517803320764195 1103 40-41 1.5282881982756997 1103 42-43 1.7658702541514621 1103 44-45 1.4086813128924334 1103 46-47 1.270374583020299 1103 48-49 1.0733309608679988 1103 50-51 1.087739176402252 1103 52-53 0.8841281676267698 1103 54-55 0.868448976529173 1103 56-57 0.8128629392639652 1103 58-59 0.8166468491999908 1103 60-61 0.9379401281990596 1103 62-63 0.6992177277270919 1103 64-65 0.4315802333796164 1103 66-67 0.5493352881739924 1103 68-69 0.5102858571535585 1103 70-71 0.6166176629157043 1103 72-73 0.5147816514975396 1103 74-75 0.6249633557555576 1103 76-77 0.4855408040128566 1103 78-79 0.4369768177175999 1103 80-81 0.45853958433347586 1103 82-83 0.5661180334295892 1103 84-85 0.5957374193563751 1103 86-87 0.5790308111396882 1103 88-89 0.5772381146290115 1103 90-91 0.6349684336717338 1103 92-93 0.5515061511029593 1103 94-95 0.45269471832211394 1103 96-97 0.496761351905203 1103 98-99 0.5161517012842864 1103 100-101 0.42148649305932295 1103 102-103 0.3445705890930988 1103 104-105 0.5190125570384687 1103 106-107 0.6301116182889999 1103 108-109 0.6714198861130569 1103 110-111 0.6500529561643873 1103 112-113 0.6662320220052429 1103 114-115 0.6422701213202267 1103 116-117 0.6330244102589475 1103 118-119 0.7488924129693615 1103 120-121 0.7058281428995521 1103 122-123 0.6882102375815791 1103 124-125 0.7167378402940585 1104 1 1.3474014902690215 1104 2 1.6384630369353914 1104 3 1.27864789920935 1104 4 1.2680428708386877 1104 5 0.8788173132431076 1104 6 1.1860113831890828 1104 7 0.7129583339470749 1104 8 1.2251434555411294 1104 9 1.4868787727272803 1104 10-11 1.6560885936450198 1104 12-13 1.7359972193987012 1104 14-15 1.7400740035612472 1104 16-17 1.2997014118930643 1104 18-19 1.392433623254444 1104 20-21 1.5550945602873831 1104 22-23 1.4702622640088698 1104 24-25 1.410347243105626 1104 26-27 1.5429064242660004 1104 28-29 1.7439612085800142 1104 30-31 1.6968996856214318 1104 32-33 1.6815764213897637 1104 34-35 1.6542378328430054 1104 36-37 1.476129613533402 1104 38-39 1.697299326407542 1104 40-41 1.661556213412247 1104 42-43 1.8024303218294406 1104 44-45 1.448372431854068 1104 46-47 1.3351649131509546 1104 48-49 1.2377411807366059 1104 50-51 1.1989913154382421 1104 52-53 1.153378431174513 1104 54-55 1.1665547295431047 1104 56-57 0.9806972554549347 1104 58-59 1.1597895760149228 1104 60-61 1.236041928960823 1104 62-63 1.1748209570834156 1104 64-65 1.1166447302791234 1104 66-67 1.1232499226118726 1104 68-69 1.0167249435008223 1104 70-71 1.1027119046578129 1104 72-73 0.9022000133911945 1104 74-75 0.8608715524566435 1104 76-77 0.8658828029964525 1104 78-79 0.9348839556006894 1104 80-81 1.1797367272985824 1104 82-83 1.209268799703807 1104 84-85 1.1775607995692035 1104 86-87 0.9956402183481323 1104 88-89 0.9501893056643027 1104 90-91 0.989579150328801 1104 92-93 1.0051106629169162 1104 94-95 0.9375266492806986 1104 96-97 1.074870097063208 1104 98-99 1.0130721256751976 1104 100-101 1.0866521714701527 1104 102-103 0.9910796129523618 1104 104-105 1.0712783928453327 1104 106-107 1.0353676509681513 1104 108-109 1.1983287467925052 1104 110-111 1.166233476535261 1104 112-113 1.1753257686554655 1104 114-115 1.2304948968901392 1104 116-117 1.0844382176157659 1104 118-119 1.1018718134454772 1104 120-121 1.1441310766659782 1104 122-123 1.101198996219658 1104 124-125 0.7911148882354553 1105 1 0.624128459610759 1105 2 0.8279861071969847 1105 3 0.4650027028296222 1105 4 0.6392713134244161 1105 5 0.31581105063604653 1105 6 0.398158517491904 1105 7 0.2673152286233318 1105 8 0.34293432882102337 1105 9 0.2758933361929401 1105 10-11 0.4809428533795206 1105 12-13 0.6659996484031296 1105 14-15 0.9181556709143699 1105 16-17 0.37713252849583156 1105 18-19 0.5217213441090358 1105 20-21 0.3985425848731339 1105 22-23 0.5100266505796434 1105 24-25 0.2287672813360473 1105 26-27 0.6453919294461201 1105 28-29 0.7184553420062834 1105 30-31 0.659477176143028 1105 32-33 0.7486288245200079 1105 34-35 0.6102411700838637 1105 36-37 0.29759382796168765 1105 38-39 0.4827179072353047 1105 40-41 0.5289220109177641 1105 42-43 0.8489747990126446 1105 44-45 0.6051607071553136 1105 46-47 0.14411579445712874 1105 48-49 0.2150062273629274 1105 50-51 0.29374492990243084 1105 52-53 0.20349380888470137 1105 54-55 0.2735906576868139 1105 56-57 0.22283863407336213 1105 58-59 0.3228457388215844 1105 60-61 0.2244909568310085 1105 62-63 0.2915689425938801 1105 64-65 -0.0022259938810229585 1105 66-67 0.023761069630005238 1105 68-69 -0.025975370157574673 1105 70-71 0.34781957291917465 1105 72-73 0.22734733779001814 1105 74-75 0.22281961400550188 1105 76-77 0.19614819813210715 1105 78-79 0.32954305011008245 1105 80-81 0.2903699581912953 1105 82-83 0.3254385765522265 1105 84-85 0.24633066724126707 1105 86-87 0.3209182337459069 1105 88-89 0.36308705513889805 1105 90-91 0.0018561835639161472 1105 92-93 0.2237963075007663 1105 94-95 0.07210537542629325 1105 96-97 0.055106713776872596 1105 98-99 0.1359731490253253 1105 100-101 0.12101862375077488 1105 102-103 0.06601703968619788 1105 104-105 -0.004410177104713142 1105 106-107 0.07469640087574447 1105 108-109 0.04168700382341939 1105 110-111 0.10582783279627961 1105 112-113 0.013180482508186486 1105 114-115 0.07411057558566014 1105 116-117 -0.005502271775821299 1105 118-119 0.034518424984305796 1105 120-121 0.03830701051256824 1105 122-123 -0.05906777183153622 1105 124-125 -0.08640124352219303 1106 1 0.3899849189966851 1106 2 -2.0459589935968054 1106 3 -0.15262611837926343 1106 4 0.38105435721757885 1106 5 0.5353962171246778 1106 6 0.7083404170172649 1106 7 0.7676365621062828 1106 8 0.7076307087552429 1106 9 0.9934331895599477 1106 10-11 1.1905601240705543 1106 12-13 1.18019078426234 1106 14-15 1.2602333796973397 1106 16-17 0.9257522518138614 1106 18-19 0.8903770116010143 1106 20-21 1.2877455612518425 1106 22-23 1.0059919480207782 1106 24-25 0.9859573912597881 1106 26-27 1.0463068190633287 1106 28-29 1.0318316315299363 1106 30-31 1.325249003138829 1106 32-33 1.403932721471783 1106 34-35 1.1171294378413634 1106 36-37 1.007321306564961 1106 38-39 1.316669558568801 1106 40-41 1.2753436237641118 1106 42-43 1.3790345991009545 1106 44-45 1.2977316373319567 1106 46-47 1.020405682434884 1106 48-49 0.8209588390536382 1106 50-51 0.8609389672354055 1106 52-53 0.900631900673865 1106 54-55 0.8508453111931367 1106 56-57 0.787767853269429 1106 58-59 0.7536568471962184 1106 60-61 0.9084348845025545 1106 62-63 0.7074721082751729 1106 64-65 0.7964416380005055 1106 66-67 0.6562360806690108 1106 68-69 0.6517537420730619 1106 70-71 0.7643992876683079 1106 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2 156728.0 3 20304.0 4 13871.0 5 11223.0 6 10309.0 7 10947.0 8 11603.0 9 12020.0 10 13076.0 11 14304.0 12 15651.0 13 17301.0 14 18136.0 15 15550.0 16 12778.0 17 10803.0 18 9686.0 19 9078.0 20 8643.0 21 8802.0 22 10390.0 23 14654.0 24 23241.0 25 28624.0 26 32212.0 27 30688.0 28 34589.0 29 38755.0 30 44771.0 31 51369.0 32 62552.0 33 77324.0 34 99050.0 35 132355.0 36 180779.0 37 205400.0 38 54906.0 39 102.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.146681692039536 19.679069620486462 13.350377301857282 21.823871385616716 2 12.451985035596762 23.957203545254412 40.67217328257395 22.918638136574874 3 19.903118600648863 29.971035729628152 26.882348135243905 23.243497534479076 4 10.781491280310735 18.84364640592802 36.757943639554554 33.616918674206694 5 11.839615771656174 42.958922020177525 31.616462297132458 13.584999911033846 6 28.69383441914362 38.42240928079745 18.6117649382586 14.271991361800326 7 26.515071216724657 36.589168211039826 19.48800326540286 17.407757306832657 8 24.66250505172838 37.74243359692276 19.335383424538698 18.259677926810163 9 25.930328532444502 20.453405577034715 19.302613963517455 34.31365192700332 10-11 22.5623166974576 31.324363232827817 25.91174770926297 20.20157236045161 12-13 24.683360030477182 28.557883783368553 26.148791423752265 20.609964762402 14-15 22.033799033326193 29.03318470287564 25.54193191955068 23.39108434424749 16-17 20.47638471305732 32.33968644530556 26.297771695866416 20.886157145770696 18-19 20.42179417997078 31.07302675739349 28.480863133234184 20.024315929401546 20-21 22.36834572330475 29.358342716019486 27.86957199816009 20.403739562515668 22-23 21.25639032468477 29.908463761667463 26.948559490656486 21.88658642299128 24-25 21.11359778022825 29.481672634343482 27.920105855102108 21.484623730326163 26-27 21.686412586114027 28.811307063095192 28.052298590963147 21.449981759827633 28-29 20.92638560578769 29.008884524214896 28.698115349121828 21.366614520875586 30-31 21.258954944050103 29.692916959956843 28.288544907934295 20.75958318805876 32-33 20.513878564657002 29.57988684970913 28.180365235950244 21.725869349683627 34-35 21.120498540244903 29.179872878289714 28.327636407173657 21.371992174291734 36-37 21.744084309688112 28.878074613021425 28.124642295623342 21.253198781667116 38-39 21.91014803571599 28.606583510984308 28.4370980643255 21.0461703889742 40-41 21.501877552632358 28.983488726456542 28.629304301751173 20.885329419159923 42-43 20.9598441940106 29.24250402671148 28.445131178091792 21.352520601186132 44-45 21.60252610025049 29.5256719083203 27.54579017286995 21.326011818559255 46-47 21.177857928781833 29.365595483035893 27.280331700160687 22.17621488802159 48-49 21.624433641947896 29.16874723276485 27.55968839863186 21.64713072665539 50-51 21.69008024127649 29.821911210882707 27.328549735643286 21.159458812197514 52-53 21.223502825751055 29.932297794940233 27.359673228937165 21.484526150371547 54-55 20.888010119735952 29.664413632692405 27.59388144183208 21.85369480573956 56-57 21.552327092442173 29.985557452716282 26.785365324069048 21.676750130772497 58-59 21.555508193896003 29.419122401604508 27.165200757523433 21.860168646976057 60-61 21.60331445732998 29.28982258311225 27.466838157048567 21.6400248025092 62-63 21.790663437211794 29.160707918961908 27.556713902487477 21.49191474133882 64-65 21.43506474285395 29.448641501863186 27.63771628915076 21.478577466132105 66-67 21.18436028292949 29.70730037400124 27.52626772543251 21.582071617636757 68-69 21.642311330000013 29.84429816772625 27.217598457254184 21.295792045019553 70-71 21.060250837698728 29.934414659966173 27.067543067202365 21.937791435132734 72-73 21.530791669296974 29.495257559497507 26.829870536371864 22.14408023483366 74-75 21.707271924422567 29.5157848082995 26.976590341999522 21.800352925278414 76-77 21.813720573295978 29.162442082221624 27.618669577368355 21.405167767114047 78-79 21.598431608830982 29.226085389308842 27.74530942902774 21.430173572832445 80-81 21.16335200734681 30.168059542824366 27.36307623891861 21.305512210910223 82-83 21.116411683304644 30.15595041401149 26.926650906391263 21.800986996292597 84-85 21.411006889048725 29.579102703623565 26.809407151100284 22.200483256227425 86-87 21.724471212428252 29.30833243414664 27.242955420516722 21.72424093290839 88-89 22.35185214425567 28.779717730899595 27.368602496894674 21.49982762795006 90-91 21.671643126334978 29.459029560762474 27.546661298800057 21.32266601410249 92-93 21.081832250382774 29.93189827994231 27.586146784615362 21.400122685059557 94-95 20.792779858455432 30.180339043718796 27.22219444086211 21.80468665696366 96-97 21.30593778238011 29.6060173545653 26.943391906663372 22.14465295639122 98-99 21.687914881271716 29.317108839386652 26.789755991180776 22.205220288160852 100-101 21.468828824088476 29.829813962818875 27.012223797105356 21.689133415987293 102-103 21.492772323417224 29.618406048758278 27.444904963405957 21.443916664418538 104-105 21.149261162474538 29.554980863953528 27.486913233041466 21.80884474053047 106-107 20.938964634829365 30.19544853447309 27.35769722693887 21.507889603758674 108-109 20.867656055732077 30.177024333445168 27.01074908067073 21.944570530152028 110-111 20.596973502444698 30.079909295173117 27.29825952879132 22.024857673590866 112-113 21.366771596896942 29.93105774801357 26.787786884006177 21.914383771083315 114-115 21.03948855771513 30.136994408565947 26.847136124425354 21.976380909293564 116-117 20.86328356834835 30.006592586381785 27.157214278785847 21.972909566484013 118-119 20.705287817630357 30.422950455352087 27.161812887144617 21.709948839872943 120-121 20.689553154329452 30.560374115277916 26.75794700756758 21.992125722825048 122-123 20.57030400978566 30.33985107778711 26.648954272758807 22.440890639668417 124-125 20.577466932910973 30.28040154972336 26.994588199783188 22.147543317582482 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11608.0 1 9103.0 2 5151.5 3 2838.0 4 1584.5 5 1020.5 6 874.0 7 1038.5 8 1349.0 9 1449.0 10 1238.0 11 1131.5 12 1169.0 13 1220.0 14 1353.0 15 1541.0 16 1742.5 17 1947.5 18 2226.5 19 2539.0 20 2980.0 21 3614.0 22 4396.5 23 5489.0 24 6682.5 25 8148.0 26 10221.0 27 12689.0 28 15042.0 29 17689.0 30 20797.0 31 23660.5 32 27053.5 33 30798.5 34 34218.0 35 37533.0 36 40724.5 37 43775.0 38 46133.0 39 47424.0 40 48084.0 41 49152.5 42 50231.5 43 50968.5 44 52199.5 45 53436.0 46 54736.5 47 59028.0 48 71965.0 49 73865.5 50 67849.0 51 61358.5 52 49019.5 53 42524.5 54 38635.5 55 34878.0 56 31038.0 57 27290.0 58 23869.5 59 20790.5 60 17442.0 61 13999.5 62 10825.5 63 7963.0 64 5752.5 65 4451.0 66 3441.0 67 2603.0 68 2004.0 69 1517.0 70 1181.5 71 986.5 72 832.5 73 747.5 74 616.5 75 487.5 76 377.0 77 250.5 78 180.5 79 145.5 80 110.0 81 77.0 82 67.5 83 47.5 84 37.5 85 31.5 86 19.5 87 15.5 88 14.5 89 12.5 90 7.0 91 4.5 92 5.0 93 3.5 94 1.0 95 1.0 96 2.5 97 2.0 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2210729987507996 2 0.2651431063449133 3 0.3180140998073 4 0.30409031022465904 5 0.3377832538845403 6 0.24557098702591793 7 0.23768959669612116 8 0.21621280804742493 9 0.21489924299245883 10-11 0.15601868940360206 12-13 0.18051667767872037 14-15 0.1992349797119877 16-17 0.16971260510162395 18-19 0.19664068872842963 20-21 0.1925029588052863 22-23 0.21575306027818683 24-25 0.20613119625055992 26-27 0.259297741850314 28-29 0.1836692338106391 30-31 0.1618312147718272 32-33 0.20297864011864117 34-35 0.16130578874984072 36-37 0.1618312147718272 38-39 0.19535996279983764 40-41 0.21732933834414614 42-43 0.2808073696253844 44-45 0.1813376558380742 46-47 0.16593610556859634 48-49 0.16780793577192307 50-51 0.17457279580499865 52-53 0.1955898366844567 54-55 0.18202727749193143 56-57 0.1812062993325776 58-59 0.15480364172775837 60-61 0.1685632356785286 62-63 0.15762780659593556 64-65 0.1529646506508058 66-67 0.13953344796377715 68-69 0.14239045195832845 70-71 0.14373685613966874 72-73 0.12071662855138732 74-75 0.12186599797448269 76-77 0.14498474294188657 78-79 0.15040319879362185 80-81 0.16143714525533734 82-83 0.1646882187663785 84-85 0.14859704684304342 86-87 0.176149073870958 88-89 0.1711575266620867 90-91 0.15874433689265677 92-93 0.15903988903002417 94-95 0.18192876011280898 96-97 0.16882594868952183 98-99 0.16324329720591577 100-101 0.14311291273855983 102-103 0.18330800342052342 104-105 0.14087985214511742 106-107 0.14780890781006376 108-109 0.16051764971686105 110-111 0.13381943997467446 112-113 0.1235407934195645 114-115 0.12183315884810852 116-117 0.12652915391961245 118-119 0.10922293432043369 120-121 0.10150573962250768 122-123 0.1301414578207693 124-125 0.1258066931393811 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1522574.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.24387354521582 #Duplication Level Percentage of deduplicated Percentage of total 1 82.78357274640258 55.66688097380269 2 10.160442493960238 13.66455020454599 3 3.3359623273054884 6.729690866668022 4 1.467733707733609 3.9478439936355825 5 0.7454602634098032 2.506381784285604 6 0.4399365681740359 1.7749823374926654 7 0.26559695343380035 1.2501837565487934 8 0.1688068233227175 0.9080979744865924 9 0.11874493923472007 0.7186382710230468 >10 0.4723653279934217 5.275756471660739 >50 0.022066180484144774 1.040206110469647 >100 0.015570789415185797 2.1012477977210104 >500 0.0015165726203947515 0.7044793425449964 >1k 0.0021232016685526524 2.9529391781605265 >5k 0.0 0.0 >10k+ 1.0110484135965011E-4 0.7581209369541841 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11151 0.7323781963963656 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4764 0.31289119609293214 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4384 0.2879334600485757 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4356 0.28609446897162305 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4135 0.27157957511424735 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2500 0.16419563187076622 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.13227600103508924 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1896 0.1245259672107891 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1793 0.11776110717771353 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1769 0.11618482911175418 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1686 0.11073353413364473 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1572 0.1032462133203378 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1532 0.10061908321040552 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 6.567825274830649E-5 0.0 5 0.0 0.0 0.0 6.567825274830649E-5 0.0 6 0.0 0.0 0.0 6.567825274830649E-5 0.0 7 0.0 0.0 0.0 6.567825274830649E-5 0.0 8 0.0 0.0 0.0 6.567825274830649E-5 0.0 9 0.0 0.0 0.0 6.567825274830649E-5 0.0 10-11 0.0 0.0 0.0 1.3135650549661297E-4 0.0 12-13 0.0 0.0 0.0 1.3135650549661297E-4 0.0 14-15 0.0 0.0 0.0 1.3135650549661297E-4 0.0 16-17 0.0 0.0 0.0 2.2987388461907272E-4 0.0 18-19 0.0 0.0 0.0 3.6123039011568567E-4 0.0 20-21 0.0 0.0 0.0 7.224607802313713E-4 0.0 22-23 0.0 0.0 0.0 0.0012478868022178231 0.0 24-25 0.0 0.0 0.0 0.0016419563187076622 0.0 26-27 0.0 0.0 0.0 0.0025942909835581065 0.0 28-29 0.0 0.0 0.0 0.004137729923143308 0.0 30-31 0.0 0.0 0.0 0.00729028605506202 0.0 32-33 0.0 0.0 0.0 0.013890950456266822 0.0 34-35 0.0 0.0 0.0 0.02239628418717251 0.0 36-37 0.0 0.0 0.0 0.035794647747827035 0.0 38-39 0.0 0.0 0.0 0.057205758143774946 0.0 40-41 0.0 0.0 0.0 0.08784466305085992 0.0 42-43 0.0 0.0 0.0 0.11490410318316219 0.0 44-45 0.0 0.0 0.0 0.14478770818364164 0.0 46-47 0.0 0.0 0.0 0.17917027349738007 0.0 48-49 0.0 0.0 0.0 0.21890561641010553 0.0 50-51 0.0 0.0 0.0 0.2648475542075459 0.0 52-53 0.0 0.0 0.0 0.30973863996101336 0.0 54-55 0.0 0.0 0.0 0.35462972571448087 0.0 56-57 0.0 0.0 0.0 0.4111787013307727 0.0 58-59 0.0 0.0 0.0 0.47974679720000474 0.0 60-61 0.0 0.0 0.0 0.5585935396243467 0.0 62-63 0.0 0.0 0.0 0.6333353912519194 0.0 64-65 0.0 0.0 0.0 0.7070592299618934 0.0 66-67 0.0 0.0 0.0 0.7885659416225419 0.0 68-69 0.0 0.0 0.0 0.8934869503879614 0.0 70-71 0.0 0.0 0.0 1.0207057259614312 0.0 72-73 0.0 0.0 0.0 1.154196774672364 0.0 74-75 0.0 0.0 0.0 1.2777375680919287 0.0 76-77 0.0 0.0 0.0 1.4026904439455816 0.0 78-79 0.0 0.0 0.0 1.5571328552832244 0.0 80-81 0.0 0.0 0.0 1.7399154326817614 0.0 82-83 0.0 0.0 0.0 1.9403326209432186 0.0 84-85 0.0 0.0 0.0 2.140881165710172 0.0 86-87 0.0 0.0 0.0 2.350854539746508 0.0 88-89 0.0 0.0 0.0 2.5853587411843364 0.0 90-91 0.0 0.0 0.0 2.8542455079359033 0.0 92-93 0.0 0.0 0.0 3.1428357505119617 0.0 94-95 0.0 0.0 0.0 3.4212458639120333 0.0 96-97 0.0 0.0 0.0 3.7033011203396353 0.0 98-99 0.0 0.0 0.0 4.030411658152576 0.0 100-101 0.0 0.0 0.0 4.426287326592993 0.0 102-103 0.0 0.0 0.0 4.836382336753418 0.0 104-105 0.0 0.0 0.0 5.239843843386266 0.0 106-107 0.0 0.0 0.0 5.637394307271765 0.0 108-109 0.0 0.0 0.0 6.05990250720162 0.0 110-111 0.0 0.0 0.0 6.556922684874429 0.0 112-113 0.0 0.0 0.0 7.117519411207599 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGC 995 0.0 32.333694 1 ACGCATA 310 0.0 28.7995 7 CGCATAG 320 0.0 27.894924 8 GTTATCA 1285 0.0 27.3548 1 TAGCCCT 1490 0.0 27.189878 4 TATAACG 115 9.973258E-4 25.9034 2 AACGCAT 345 0.0 25.880371 6 CGCAGAT 510 0.0 25.670622 8 TACCTGG 1720 0.0 25.632318 2 CATGGGG 3345 0.0 25.113523 4 GTACATG 18625 0.0 24.886738 1 TACATGG 18365 0.0 24.460485 2 ATAGCCC 975 0.0 24.442184 3 CGATCGT 75 1.6750259E-4 23.826025 24-25 TCGCGGA 75 1.6900552E-4 23.794657 82-83 TTATCAA 1480 0.0 22.945515 2 GTACCTG 2200 0.0 22.477089 1 ACATGGG 18950 0.0 22.322037 3 GTGTAGC 1265 0.0 22.135635 1 ATGGGAG 2945 0.0 21.648415 5 >>END_MODULE