FastQCFastQC Report
Thu 26 May 2016
SRR1364628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1522574
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117470.7715224350343562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106840.7017064523629065No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72700.4774808974801882No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA56220.3692431369509791No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT53820.3534803562913855No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA52670.34592735722533025No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46470.30520684052138025No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT33570.2204818944760649No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29250.1921088892887965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25810.16951557034337905No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG24790.16281638856305178No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG24640.1618312147718272No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA21110.13864679155167497No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC18780.12334375866131957No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT18640.1224242631228433No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC16570.10882886480394385No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC16090.10567630867202513No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15720.1032462133203378No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC255.3343974E-447.599277116-117
GCGTATC1001.0556063E-535.7334981
AACGCCG2650.033.7086336
GCCGAGT2750.032.4859
ACCGCAC850.007180453728.0244338
ACGCCGA3500.027.2237347
CGCCGAG3350.026.6650378
GAGTACT122000.026.45319212-13
TAACGCA2256.548362E-926.446654
GTAGGAC7200.026.445783
AGTACTT122600.026.03232412-13
GTACTTT130350.024.87284714-15
TAGCCCT14650.023.9644554
TACTTTT139600.022.88352614-15
ACTTTTT149900.022.46383516-17
CAACGCC4000.022.3407825
GTAGCCC11000.022.1783923
TATAACG1703.9208983E-421.0107252
GTCCTAC6850.020.8662761
ATGGGAG26850.020.8569475
Produced by FastQC (version 0.11.5)