FastQCFastQC Report
Thu 26 May 2016
SRR1364627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895006
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG104350.036.360511
GTATCAA70950.034.336331
TACATGG105600.034.2931672
ACATGGG112000.031.6422163
TCAACGC80800.030.2226754
ATCAACG80950.030.0936183
CAACGCA81100.029.9660725
AACGCAG82900.028.5726536
TATCAAC89050.027.0218682
GGTATCA49550.024.642981
ACGCAGA96550.024.2831947
CATGGGG55500.023.7169974
CGCAGAG97300.022.8093418
CATGGGA61550.021.772834
GAGTACA79900.020.7243541
CGCGGAA3650.020.37300182-83
GTGTAGC18050.020.1296221
AGTACAT77400.020.0847992
GCAGAGT105600.019.9463589
CGATCGT3650.019.58196624-25
Produced by FastQC (version 0.11.5)