FastQCFastQC Report
Thu 26 May 2016
SRR1364627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2895006
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65010.2245591200847252No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55840.19288388348763355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.12721908002954052No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102850.041.4748421
GGTATCA67250.040.928491
ATCAACG124850.033.358573
TCAACGC126100.033.0296024
CAACGCA126650.032.6386875
TATCAAC129800.032.2920882
AACGCAG131500.031.7895666
GTACATG101450.030.125991
TACATGG104900.028.6057382
ACGCAGA153000.026.8558187
ACATGGG108900.026.607193
CGCAGAG154600.026.539368
CATGGGG56050.024.0974274
GCAGAGT169000.023.6441699
GTACCGT2302.3476059E-723.3017166
GAGTACT92450.023.00102412-13
AGAGTAC148700.022.00005510-11
AGTACTT98150.021.99903112-13
GTACTTT97600.021.87888714-15
GTATACG2357.4002273E-620.2554824
Produced by FastQC (version 0.11.5)