##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364627_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2895006 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.5941262297902 31.0 30.0 33.0 26.0 34.0 2 29.832723662748887 31.0 30.0 34.0 26.0 34.0 3 29.833298791090588 31.0 30.0 34.0 26.0 34.0 4 33.51381966047739 35.0 33.0 37.0 28.0 37.0 5 32.980983804524065 35.0 33.0 37.0 26.0 37.0 6 33.000391709032726 35.0 33.0 37.0 26.0 37.0 7 32.80024704612011 35.0 33.0 37.0 25.0 37.0 8 32.73897394340461 35.0 33.0 37.0 25.0 37.0 9 34.06237430941421 38.0 34.0 39.0 25.0 39.0 10-11 33.85598959034972 38.0 33.0 39.0 24.0 39.0 12-13 33.73026670756468 37.5 33.0 39.0 23.0 39.0 14-15 34.72675773383544 39.0 33.0 41.0 24.0 41.0 16-17 34.63116622210801 38.5 33.0 41.0 23.0 41.0 18-19 34.66087583238169 38.5 33.5 41.0 22.5 41.0 20-21 34.6727098320349 39.0 34.0 41.0 22.0 41.0 22-23 34.65777825676354 39.0 34.0 41.0 23.0 41.0 24-25 34.64603734845454 39.0 34.0 41.0 23.0 41.0 26-27 34.54924687548143 39.0 33.5 41.0 22.5 41.0 28-29 34.32818809356527 39.0 33.0 41.0 19.5 41.0 30-31 34.278817211432376 38.5 33.0 41.0 20.5 41.0 32-33 34.12282962453273 38.0 33.0 41.0 19.0 41.0 34-35 34.00329429369059 38.0 33.0 41.0 18.5 41.0 36-37 33.848010850409295 38.0 33.0 41.0 17.5 41.0 38-39 33.727272067829915 38.0 33.0 40.0 16.0 41.0 40-41 33.56999933678894 38.0 33.0 40.0 15.5 41.0 42-43 33.41402073087241 38.0 32.5 40.0 14.5 41.0 44-45 33.23242922467173 38.0 32.0 40.0 10.5 41.0 46-47 33.00809013867328 38.0 32.0 40.0 8.5 41.0 48-49 32.78974257739017 38.0 31.5 40.0 7.5 41.0 50-51 32.608778185606525 38.0 31.0 40.0 3.5 41.0 52-53 32.45835189978881 38.0 31.0 40.0 2.0 41.0 54-55 32.25076666507772 37.5 31.0 40.0 2.0 41.0 56-57 32.03771874738774 37.0 31.0 40.0 2.0 41.0 58-59 31.851252812602116 37.0 30.5 40.0 2.0 41.0 60-61 31.636290218396784 36.5 30.0 40.0 2.0 41.0 62-63 31.377824432833645 36.0 30.0 40.0 2.0 41.0 64-65 31.10867507701193 36.0 29.5 39.0 2.0 41.0 66-67 30.830107433283388 35.0 29.0 39.0 2.0 41.0 68-69 30.516326736455813 35.0 29.0 39.0 2.0 40.5 70-71 30.256934182519828 35.0 29.0 38.0 2.0 40.0 72-73 30.094577869614085 35.0 29.0 37.5 2.0 40.0 74-75 29.809511966469152 35.0 29.0 37.0 2.0 39.0 76-77 29.505458019776125 35.0 29.0 36.5 2.0 39.0 78-79 29.190148483284666 34.0 29.0 36.0 2.0 39.0 80-81 28.90680882872091 34.0 29.0 36.0 2.0 37.5 82-83 28.648184321552357 34.0 28.5 35.0 2.0 37.0 84-85 28.623534286284723 34.0 29.0 35.0 2.0 37.0 86-87 28.47655600713781 34.0 29.0 35.0 2.0 36.0 88-89 28.333740931797724 34.0 29.0 35.0 2.0 36.0 90-91 28.17794142740982 34.0 29.0 35.0 2.0 36.0 92-93 28.04467797303356 34.0 28.5 35.0 2.0 35.5 94-95 27.95666882901106 34.0 28.0 35.0 2.0 35.0 96-97 27.877158458393524 34.0 28.0 35.0 2.0 35.0 98-99 27.79119041549482 34.0 28.0 35.0 2.0 35.0 100-101 27.70045226158426 34.0 27.0 35.0 2.0 35.0 102-103 26.519856953664345 32.5 25.0 34.5 2.0 35.0 104-105 27.45725432002559 34.0 27.0 35.0 2.0 35.0 106-107 27.628688334324696 34.0 27.0 35.0 2.0 35.0 108-109 27.64441230864461 34.0 27.5 35.0 2.0 35.0 110-111 27.58940914112095 34.0 27.0 35.0 2.0 35.0 112-113 27.544289027380255 34.0 27.0 35.0 2.0 35.0 114-115 27.473853422065446 34.0 27.0 35.0 2.0 35.0 116-117 27.35965866737409 34.0 27.0 35.0 2.0 35.0 118-119 27.255536603378367 34.0 27.0 35.0 2.0 35.0 120-121 27.124449655717466 34.0 27.0 35.0 2.0 35.0 122-123 26.9397588467865 34.0 27.0 35.0 2.0 35.0 124-125 26.136565347360246 33.0 24.5 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.824883593539596 1101 2 0.8753717264824097 1101 3 0.7761059120452813 1101 4 0.8131735840790029 1101 5 1.22512343991108 1101 6 1.1325466608589494 1101 7 1.1426552610320257 1101 8 1.2454096690051344 1101 9 1.350181620001912 1101 10-11 1.384809173439102 1101 12-13 1.3545040462774054 1101 14-15 1.4779346948880914 1101 16-17 1.5528147150039828 1101 18-19 1.5840545199310725 1101 20-21 1.5982961505724305 1101 22-23 1.4447875336789764 1101 24-25 1.3767064791743593 1101 26-27 1.4482365355147948 1101 28-29 1.5237201577794153 1101 30-31 1.4543691553427749 1101 32-33 1.6340683000544445 1101 34-35 1.5494324578669918 1101 36-37 1.5539862068335495 1101 38-39 1.5877301827366352 1101 40-41 1.4894235915185234 1101 42-43 1.6105733785707343 1101 44-45 1.5371108338862172 1101 46-47 1.6074482804139691 1101 48-49 1.6344304987329252 1101 50-51 1.6212254458374176 1101 52-53 1.675088506498433 1101 54-55 1.5342646778199907 1101 56-57 1.5934205773530365 1101 58-59 1.5898261388600972 1101 60-61 1.6144440673072324 1101 62-63 1.5587164654031476 1101 64-65 1.4931906976303182 1101 66-67 1.5248843062573556 1101 68-69 1.529022238327137 1101 70-71 1.4957354806869425 1101 72-73 1.4686156170729667 1101 74-75 1.348519193353411 1101 76-77 1.443573591166249 1101 78-79 1.4200678607829467 1101 80-81 1.4131567866920598 1101 82-83 1.3262518476815792 1101 84-85 1.437614835996282 1101 86-87 1.3537209907170862 1101 88-89 1.3251210062462313 1101 90-91 1.2977709110495539 1101 92-93 1.309896168962382 1101 94-95 1.2909830871263175 1101 96-97 1.3042228310265251 1101 98-99 1.3795776488732407 1101 100-101 1.4029198797847506 1101 102-103 1.4305160082622308 1101 104-105 1.3540458479809026 1101 106-107 1.4024432839176093 1101 108-109 1.3805549454183712 1101 110-111 1.4043466331231684 1101 112-113 1.4435951471897397 1101 114-115 1.4017116577611866 1101 116-117 1.375341925516949 1101 118-119 1.3985340061614764 1101 120-121 1.4106315418160378 1101 122-123 1.33187153008004 1101 124-125 1.2340733310745122 1102 1 0.5652555414161178 1102 2 -0.4474705899907043 1102 3 0.2140645471942726 1102 4 0.5208470812595394 1102 5 0.8626887656963973 1102 6 0.6900986821276618 1102 7 0.698074101996518 1102 8 0.595892516195839 1102 9 0.8297375981115493 1102 10-11 0.9941205892365943 1102 12-13 1.0424324313106368 1102 14-15 1.1998455945745832 1102 16-17 1.204500165923406 1102 18-19 1.2058684532174055 1102 20-21 1.11845638738518 1102 22-23 1.200297218068009 1102 24-25 1.1353553080081085 1102 26-27 1.127095017649765 1102 28-29 1.2062163515475959 1102 30-31 1.0260002974306843 1102 32-33 1.0338478930571782 1102 34-35 0.9631463736296411 1102 36-37 0.9144127010144345 1102 38-39 0.8431909638200352 1102 40-41 0.9510797271199394 1102 42-43 0.8447127963607741 1102 44-45 0.7298768727743123 1102 46-47 0.7201168678938075 1102 48-49 0.8661155692933065 1102 50-51 0.7794311865723174 1102 52-53 0.9121831279045836 1102 54-55 0.9873701308052247 1102 56-57 0.806877116829348 1102 58-59 0.8690359147717643 1102 60-61 0.8007656366284053 1102 62-63 0.8935829827738537 1102 64-65 0.8205388600357821 1102 66-67 0.7192264956674066 1102 68-69 0.5914509304399225 1102 70-71 0.45508903533591294 1102 72-73 0.4675590893294199 1102 74-75 0.6312847477365295 1102 76-77 0.716227959456539 1102 78-79 0.771055388075343 1102 80-81 0.6899052471561227 1102 82-83 0.5886202458422325 1102 84-85 0.6753516906292418 1102 86-87 0.6436075858631725 1102 88-89 0.7439922837399351 1102 90-91 0.7318585627787257 1102 92-93 0.7781182022461124 1102 94-95 0.6945123109015583 1102 96-97 0.6625755634257757 1102 98-99 0.7119694929489739 1102 100-101 0.5101331324598277 1102 102-103 0.504683355273496 1102 104-105 0.5859905789943589 1102 106-107 0.8203944799279448 1102 108-109 0.8142512952343814 1102 110-111 0.7362465512000682 1102 112-113 0.7337312649122687 1102 114-115 0.8218947463037907 1102 116-117 0.7923788510848624 1102 118-119 0.7962568680090847 1102 120-121 0.869203197007284 1102 122-123 0.8265654962623934 1102 124-125 0.6505635408289443 1103 1 0.4796209385427339 1103 2 -0.8109697305820163 1103 3 0.252605063726854 1103 4 0.39892427968427313 1103 5 0.7791494962577161 1103 6 0.7363782674854988 1103 7 0.6963591360531964 1103 8 0.857185725611636 1103 9 0.9093447088422408 1103 10-11 1.1075859527633085 1103 12-13 1.092257876678751 1103 14-15 1.150212335697141 1103 16-17 1.2632145638574315 1103 18-19 1.2307612639945162 1103 20-21 1.2269359172515735 1103 22-23 1.1223468396185297 1103 24-25 0.9737490387384327 1103 26-27 1.0633233034648981 1103 28-29 1.1202755597762248 1103 30-31 1.1041407055685397 1103 32-33 1.0390686641109212 1103 34-35 1.0850200309239213 1103 36-37 1.2069309387036 1103 38-39 1.2120085477978293 1103 40-41 1.2545441986548482 1103 42-43 1.1648884032040598 1103 44-45 1.1612272420727834 1103 46-47 1.2916467540100243 1103 48-49 1.2486790705598878 1103 50-51 1.277452551547789 1103 52-53 1.1649028941195425 1103 54-55 1.1731672487319358 1103 56-57 1.232823694993165 1103 58-59 1.2413171691864662 1103 60-61 1.1972342734107286 1103 62-63 1.1753052695204182 1103 64-65 1.1775427134712046 1103 66-67 1.193284015427949 1103 68-69 1.1777800236539875 1103 70-71 1.2436129521936472 1103 72-73 1.2598574872412698 1103 74-75 1.1871416907373273 1103 76-77 1.1196032164048724 1103 78-79 1.1278551155787753 1103 80-81 1.1885875179793182 1103 82-83 1.1598477174644906 1103 84-85 1.2051685580864984 1103 86-87 1.1496695098140144 1103 88-89 1.0831931977311307 1103 90-91 1.1092277908484007 1103 92-93 1.1125532764116102 1103 94-95 1.1091701858258176 1103 96-97 1.0483293604377089 1103 98-99 1.0982413076562416 1103 100-101 1.1664499981454277 1103 102-103 1.2261017578193858 1103 104-105 1.2064056987330893 1103 106-107 1.1598651482639752 1103 108-109 1.1343562668023353 1103 110-111 1.11504181073348 1103 112-113 1.0981587548365397 1103 114-115 1.0739962035164545 1103 116-117 1.106454558851091 1103 118-119 1.1471081347683985 1103 120-121 1.040969776770222 1103 122-123 1.1326205954745312 1103 124-125 1.085892517113514 1104 1 0.31558470820894513 1104 2 -0.7735923797160531 1104 3 0.09141967633928871 1104 4 0.3968956361523226 1104 5 0.7570503105736464 1104 6 0.596701255699692 1104 7 0.7799660594386495 1104 8 0.8461646427035419 1104 9 0.7845477763005064 1104 10-11 1.0184982766674509 1104 12-13 0.8870429960278088 1104 14-15 0.98979573861277 1104 16-17 1.1173944511596972 1104 18-19 1.1027772367790334 1104 20-21 1.1490360206696693 1104 22-23 1.0611569237401426 1104 24-25 1.0232074553072295 1104 26-27 0.9582881933684035 1104 28-29 1.19780520333736 1104 30-31 1.0777920352447836 1104 32-33 1.1070337137904218 1104 34-35 1.0807701701637313 1104 36-37 1.1668946415708064 1104 38-39 1.2210908047495153 1104 40-41 1.164140025582853 1104 42-43 1.2238882446316595 1104 44-45 1.106766121949832 1104 46-47 1.180953512775062 1104 48-49 1.2099759701534651 1104 50-51 1.2297081913242494 1104 52-53 0.8970230282110165 1104 54-55 1.1846165072178891 1104 56-57 1.188405744476789 1104 58-59 1.1180902388382705 1104 60-61 1.1467246000427949 1104 62-63 1.1150929660471398 1104 64-65 1.1858738577832284 1104 66-67 1.1552982520402395 1104 68-69 1.2385647073050556 1104 70-71 1.227182052901565 1104 72-73 1.1830855008165102 1104 74-75 1.1196201623521134 1104 76-77 1.0603273025298456 1104 78-79 1.0946048597416151 1104 80-81 0.9590216182073092 1104 82-83 1.0204511411239174 1104 84-85 1.0305031624062302 1104 86-87 1.080349297970912 1104 88-89 1.0513947670262134 1104 90-91 1.0497974418958336 1104 92-93 1.0825587196274924 1104 94-95 1.0526341593493385 1104 96-97 1.0591307889734587 1104 98-99 1.064591235726958 1104 100-101 1.0710981103364574 1104 102-103 1.0332714721152456 1104 104-105 1.0387733802932253 1104 106-107 1.1051415712557109 1104 108-109 1.107708881577011 1104 110-111 1.036127592385597 1104 112-113 1.0655662585307937 1104 114-115 1.1211889454138415 1104 116-117 1.1371681828082174 1104 118-119 1.0294610272704539 1104 120-121 1.056936844260985 1104 122-123 1.0931677364622487 1104 124-125 1.0731357885434285 1105 1 0.5013106183855989 1105 2 0.18662758511057476 1105 3 0.5013207184160926 1105 4 0.4523934443786928 1105 5 0.5832833472956978 1105 6 0.46019444413972366 1105 7 0.6151971799993277 1105 8 0.6445765911509298 1105 9 0.7036411432322822 1105 10-11 0.7952037915373964 1105 12-13 0.7945685229721917 1105 14-15 0.8768235885747089 1105 16-17 0.9969125234488558 1105 18-19 0.9563733204601732 1105 20-21 0.9815818904231932 1105 22-23 0.8104652682292084 1105 24-25 0.9182254357256312 1105 26-27 0.9305432265731213 1105 28-29 1.1060040116424759 1105 30-31 0.9628958491933872 1105 32-33 0.9560940072518491 1105 34-35 0.8590099265749984 1105 36-37 0.9797709835399928 1105 38-39 1.0408600779206267 1105 40-41 0.9284994239606803 1105 42-43 0.9078187378463483 1105 44-45 0.9267361983337992 1105 46-47 1.096440296511311 1105 48-49 1.0104445109834543 1105 50-51 1.0217377474572729 1105 52-53 0.9601303594969366 1105 54-55 0.8833342540221025 1105 56-57 0.9413141099648854 1105 58-59 0.851809429325705 1105 60-61 0.9073934191099085 1105 62-63 0.9360408097093647 1105 64-65 0.9289586151131957 1105 66-67 0.8791059009364801 1105 68-69 0.8019043403470256 1105 70-71 0.7653254762682344 1105 72-73 0.7804986829568357 1105 74-75 0.8451694181990312 1105 76-77 0.9239464851426504 1105 78-79 0.9363063060497225 1105 80-81 0.6595426566092861 1105 82-83 0.773435628091395 1105 84-85 0.5161516386642049 1105 86-87 0.6317732946110688 1105 88-89 0.6815598718901157 1105 90-91 0.6879316781874039 1105 92-93 0.5540669748706897 1105 94-95 0.6168045664680122 1105 96-97 0.6727473467076663 1105 98-99 0.6932299736853267 1105 100-101 0.6961735755961875 1105 102-103 0.3306675925446747 1105 104-105 0.2387602559002282 1105 106-107 0.592658961076431 1105 108-109 0.5938258986649707 1105 110-111 0.5858559174505658 1105 112-113 0.45274897633009203 1105 114-115 0.640431930425784 1105 116-117 0.34125249390883283 1105 118-119 0.17302744982341167 1105 120-121 0.14827868220097784 1105 122-123 0.2690752882379783 1105 124-125 0.08882862524973589 1106 1 0.629746293089454 1106 2 0.6520615536891547 1106 3 0.726775023589564 1106 4 0.539619609846703 1106 5 0.7728845399195663 1106 6 0.7436691305934531 1106 7 0.820277668324799 1106 8 0.93775270805925 1106 9 0.9125479823274176 1106 10-11 1.0537804580757069 1106 12-13 0.8842685700819715 1106 14-15 1.044123408834956 1106 16-17 1.2767308536708484 1106 18-19 1.1674266020535526 1106 20-21 1.244198375424837 1106 22-23 1.1914994444521199 1106 24-25 1.1612809021481496 1106 26-27 1.0914124424923273 1106 28-29 1.3427437959262534 1106 30-31 1.159313675421508 1106 32-33 1.155080362528075 1106 34-35 1.1188742922305863 1106 36-37 1.2013978445784943 1106 38-39 1.2424384933680699 1106 40-41 1.262424695768921 1106 42-43 1.2325501621638395 1106 44-45 1.0943186059907788 1106 46-47 0.9607365234026801 1106 48-49 1.1544400338901681 1106 50-51 1.1563119078121389 1106 52-53 1.163650354058639 1106 54-55 1.226726762187873 1106 56-57 1.1222378800596289 1106 58-59 1.009623521093289 1106 60-61 1.0585332524525128 1106 62-63 1.053869747476437 1106 64-65 1.0376421937644338 1106 66-67 0.9339508165738408 1106 68-69 1.041386974140476 1106 70-71 1.0123187691850823 1106 72-73 1.147047076018815 1106 74-75 1.127149759490635 1106 76-77 1.1248637473368852 1106 78-79 0.9611110571498642 1106 80-81 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28-29 -4.838274063223832 2216 30-31 -4.4954574881011276 2216 32-33 -3.619375194570292 2216 34-35 -3.225400451717732 2216 36-37 -3.0942412961193426 2216 38-39 -3.143425523188501 2216 40-41 -3.571016519790561 2216 42-43 -3.6590725277508014 2216 44-45 -3.6588558000817315 2216 46-47 -3.3296595252355665 2216 48-49 -3.568026869487923 2216 50-51 -3.765523888510238 2216 52-53 -3.322901924123581 2216 54-55 -3.0998152805392003 2216 56-57 -3.3581234024138062 2216 58-59 -3.399625310226675 2216 60-61 -3.1901704549245515 2216 62-63 -2.9860142506986342 2216 64-65 -3.073845504514452 2216 66-67 -3.00866118165413 2216 68-69 -2.9903123362225905 2216 70-71 -3.6256988849914187 2216 72-73 -3.999658972024349 2216 74-75 -3.6254489220663686 2216 76-77 -3.9385375711061137 2216 78-79 -3.4872856923658055 2216 80-81 -3.1722113540859027 2216 82-83 -3.077226681276798 2216 84-85 -3.0585376067309262 2216 86-87 -3.1514574441490844 2216 88-89 -3.0100360877058456 2216 90-91 -2.9364335426484054 2216 92-93 -3.152768041964329 2216 94-95 -2.9852077131913255 2216 96-97 -3.0279129820703083 2216 98-99 -2.9844139161938585 2216 100-101 -3.2391003400306673 2216 102-103 -3.0484843187248813 2216 104-105 -2.97892918755392 2216 106-107 -3.0619249169192138 2216 108-109 -3.1507825386493487 2216 110-111 -3.1060953600301247 2216 112-113 -3.1888584706891194 2216 114-115 -3.2292198818142666 2216 116-117 -3.178925363092553 2216 118-119 -3.3316293138921758 2216 120-121 -3.338290935545107 2216 122-123 -3.4027646800725755 2216 124-125 -3.4397940723141396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103369.0 3 32791.0 4 15548.0 5 11863.0 6 12643.0 7 14737.0 8 16936.0 9 18188.0 10 19126.0 11 21630.0 12 23922.0 13 26197.0 14 27542.0 15 23033.0 16 16383.0 17 14157.0 18 12893.0 19 12123.0 20 11764.0 21 13034.0 22 15374.0 23 20103.0 24 27410.0 25 36318.0 26 44402.0 27 50809.0 28 58445.0 29 68805.0 30 79799.0 31 94918.0 32 114164.0 33 142062.0 34 183453.0 35 247135.0 36 392558.0 37 624179.0 38 246353.0 39 840.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.344831161649324 19.82742888742958 14.021256005253516 24.806483945667576 2 15.571396917018482 20.74131764222744 39.03943828385098 24.647847156903108 3 19.5114276101673 24.48215996788953 29.22539711489372 26.78101530704945 4 13.043675019276316 17.292789966919354 36.52129514734291 33.14223986646142 5 13.26457486493173 38.51795312657649 34.28204770269915 13.93542430579263 6 32.29808794341136 37.53477549898135 16.626539983792735 13.54059657381456 7 28.49114250234456 32.35452097664771 20.626596499833333 18.527740021174402 8 25.773970762064053 36.96617554505932 18.831808984161 18.42804470871563 9 25.65707458157901 16.611865114606577 19.726140772112934 38.00491953170148 10-11 24.860879882683232 27.153557987711878 27.181639497014377 20.803922632590517 12-13 25.621985246947638 24.047033170358613 28.08216882928004 22.248812753413713 14-15 22.433661434221232 25.707387114887936 25.81375059784119 26.045200853049643 16-17 22.09269473862829 29.020012258292276 26.364244769792418 22.523048233287017 18-19 21.980986541632785 27.550602728426437 28.656111463826278 21.812299266114497 20-21 22.64009533289909 26.76781364276798 28.821775393879534 21.770315630453396 22-23 22.37239231705044 26.667446267562827 28.66004051967021 22.300120895716518 24-25 22.30706948448466 26.792932380796447 28.804206277983536 22.09579185673536 26-27 22.12261390822679 27.09343262155588 28.689353320856675 22.09460014936066 28-29 21.92415299069637 27.032932055571212 28.869154949364077 22.17376000436834 30-31 22.192181985115056 26.684711534276612 28.890233733539755 22.232872747068573 32-33 21.941458447110975 26.873060598618054 28.87385440814729 22.311626546123684 34-35 22.36632670191357 26.787232910743537 28.712790232559104 22.13365015478379 36-37 22.399002141451145 26.591869877697732 28.638899592210638 22.37022838864048 38-39 22.154047349124664 26.908959774176633 28.84142554454119 22.095567332157515 40-41 22.332872539815117 26.832396893132522 28.698196134999378 22.13653443205299 42-43 22.222262522841817 26.80903724122684 28.415080261562537 22.553619974368804 44-45 22.33668945763843 26.923053009216563 28.332203801995576 22.408053731149437 46-47 22.432926246894002 27.181780609246243 27.96775547939752 22.417537664462238 48-49 22.538389889742678 27.144589439621857 27.82552196450398 22.491498706131484 50-51 22.404886228587387 27.228748527414652 27.77669448998763 22.58967075401033 52-53 22.618807673701372 27.359508422180344 27.464810503748787 22.556873400369497 54-55 22.519978376548572 27.13990131277839 27.64107488588103 22.699045424792015 56-57 22.52479612402043 27.362948380779894 27.621812195235268 22.490443299964404 58-59 22.640384855851767 27.084555265170433 27.610599079932825 22.664460799044974 60-61 22.644029062461357 27.15072093114833 27.788612527918765 22.416637478471547 62-63 22.539590591522092 27.271739678605154 27.768094435728287 22.420575294144466 64-65 22.409296334478473 27.07969162593107 27.754650662929635 22.756361376660823 66-67 22.4361711167438 27.394312826985505 27.510478389336672 22.659037666934022 68-69 22.293527498924 27.17225575529553 27.762103167338285 22.772113578442177 70-71 22.31655133011814 26.990289484719547 27.824743713829953 22.868415471332355 72-73 22.503573891601366 27.120539241822016 27.664623974098372 22.71126289247824 74-75 22.392355967987974 27.367084042623745 27.50929907368514 22.731260915703135 76-77 22.489394605644442 27.14701161213393 27.636650778293117 22.72694300392851 78-79 22.473486845150465 27.17868667741171 27.52336978035757 22.824456697080254 80-81 22.30583483838921 27.40175224946627 27.603896351751462 22.688516560393055 82-83 22.581417905756197 27.291656447905737 27.520188241666155 22.606737404671915 84-85 22.434139101064833 27.236120307992774 27.56810614704026 22.761634443902132 86-87 22.43340662073682 27.113034737538406 27.796783759557442 22.656774882167326 88-89 22.498036273499952 27.117145871200265 27.595175968547213 22.789641886752566 90-91 22.416913816413505 27.28529750888254 27.7035522551594 22.594236419544554 92-93 22.46334269316021 27.25514570257168 27.683048177286416 22.59846342698169 94-95 22.333072237704684 27.06163785546932 27.668352705362366 22.936937201463632 96-97 22.474959712729742 27.11506873448746 27.555352895760898 22.854618657021902 98-99 22.316780590943942 27.1544158272678 27.44048012791805 23.08832345387021 100-101 22.170786655023573 27.13696618159912 27.735240719145338 22.95700644423197 102-103 22.405810562923325 27.007998435925895 27.630916508391095 22.95527449275968 104-105 22.403069164844986 26.855019681418536 27.779755332445937 22.962155821290544 106-107 22.217856284816794 27.024782154596444 27.839581220051706 22.917780340535057 108-109 22.240029569523358 26.94564268374514 27.535530494002757 23.278797252728747 110-111 22.16408132839651 27.076586738625736 27.628991436552734 23.130340496425017 112-113 22.25330335911172 26.942118156834084 27.70111698651298 23.10346149754122 114-115 22.120068767053162 27.0409130805588 27.519152909283655 23.31986524310438 116-117 21.9715459335777 26.969513944385497 27.701012782101497 23.357927339935298 118-119 21.970269545801234 26.886461361601466 27.611414935832958 23.531854156764346 120-121 21.90605590464435 27.153494394369808 27.62518745601844 23.315262244967407 122-123 22.00497067337253 27.050031433723206 27.579171531409543 23.365826361494726 124-125 21.899595606560798 26.922285094093063 27.52941066746161 23.648708631884524 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 67.0 1 74.5 2 170.5 3 321.0 4 270.5 5 247.5 6 680.0 7 1432.0 8 2138.5 9 2460.5 10 2095.0 11 1618.5 12 1576.5 13 1721.0 14 1954.5 15 2207.0 16 2453.5 17 2701.5 18 2949.5 19 3211.0 20 3545.5 21 4234.0 22 5182.5 23 6385.0 24 7972.0 25 10150.5 26 13405.0 27 17658.0 28 21594.0 29 26021.5 30 32360.5 31 38052.0 32 43270.0 33 49894.0 34 57614.5 35 64628.5 36 72074.0 37 79773.0 38 83893.0 39 86678.0 40 90212.5 41 94985.0 42 100117.0 43 104884.0 44 109573.0 45 112873.5 46 112354.5 47 111643.0 48 116015.0 49 116160.0 50 110605.0 51 105049.0 52 100296.0 53 96721.0 54 92916.5 55 86698.5 56 78105.5 57 70350.5 58 63919.0 59 56947.5 60 48198.0 61 38373.0 62 29830.5 63 23162.5 64 17406.0 65 12810.5 66 9576.0 67 7440.0 68 5479.0 69 3910.5 70 3020.5 71 2425.0 72 1998.5 73 1790.5 74 1416.5 75 1024.5 76 709.5 77 436.0 78 290.5 79 205.5 80 141.0 81 88.5 82 54.0 83 31.5 84 18.0 85 10.5 86 7.0 87 6.5 88 6.0 89 2.5 90 1.0 91 1.0 92 1.0 93 2.0 94 1.5 95 0.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0589290661228336 2 0.07450761760079254 3 0.0 4 0.009050067599169051 5 0.03157160986885692 6 2.763379419593604E-4 7 3.454224274492005E-5 8 0.0 9 5.181336411738007E-4 10-11 0.05675290482990363 12-13 0.009775454696812373 14-15 0.00744385331153027 16-17 0.0502416920724862 18-19 1.381689709796802E-4 20-21 0.010621739644062914 22-23 0.012521562995033517 24-25 0.0 26-27 0.0 28-29 0.05034531880072097 30-31 0.0 32-33 0.0036269354882166047 34-35 0.0 36-37 2.245245778419803E-4 38-39 0.0 40-41 0.0 42-43 0.0033160553035123247 44-45 0.0 46-47 8.635560686230011E-5 48-49 1.8998233509706026E-4 50-51 4.317780343115006E-4 52-53 5.181336411738007E-5 54-55 3.454224274492005E-5 56-57 0.0016407565303837024 58-59 0.0 60-61 0.0 62-63 0.0 64-65 5.354047625462607E-4 66-67 0.0 68-69 6.908448548984009E-4 70-71 0.0 72-73 0.0018134677441083023 74-75 0.003246970818022484 76-77 5.872181266636408E-4 78-79 9.671827968577613E-4 80-81 0.0014335030739141819 82-83 1.381689709796802E-4 84-85 1.0362672823476015E-4 86-87 3.799646701941205E-4 88-89 0.0 90-91 0.0 92-93 0.006476670514672508 94-95 0.005302234261345227 96-97 0.005906723509381327 98-99 9.499116754853012E-4 100-101 4.8359139842888067E-4 102-103 8.635560686230011E-5 104-105 5.699470052911808E-4 106-107 0.004162340250762865 108-109 0.004611389406446826 110-111 9.326405541128412E-4 112-113 0.0025561259631240833 114-115 5.181336411738007E-4 116-117 0.0020552634433227425 118-119 0.0021416190501850427 120-121 0.011295313377588856 122-123 8.980983113679212E-4 124-125 0.0018307388654807623 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2895006.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.8704159750155 #Duplication Level Percentage of deduplicated Percentage of total 1 77.6512652566019 42.607572256160154 2 12.136473357946706 13.31866683240458 3 4.174374245961115 6.871489539338341 4 1.8961108068790578 4.161615548327047 5 1.0409476235665072 2.8558614556649045 6 0.6645213685219457 2.1877538349051435 7 0.4472397120291382 1.7178160327709473 8 0.31535707316015765 1.3843019027968995 9 0.2223097564175136 1.097840592894001 >10 1.2850427470212094 13.161939384230307 >50 0.11150386013055173 4.2410074342276864 >100 0.05276295524962966 5.023012643161066 >500 0.0015842700869621283 0.5767254641395556 >1k 3.8022482080446516E-4 0.37417959235289927 >5k 1.2674160693482172E-4 0.42021748662622327 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6501 0.2245591200847252 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5584 0.19288388348763355 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3683 0.12721908002954052 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.454224274492005E-5 0.0 0.0 0.0 0.0 3 3.454224274492005E-5 0.0 0.0 0.0 0.0 4 3.454224274492005E-5 0.0 0.0 0.0 0.0 5 3.454224274492005E-5 0.0 0.0 0.0 0.0 6 3.454224274492005E-5 0.0 0.0 0.0 0.0 7 3.454224274492005E-5 0.0 0.0 0.0 0.0 8 3.454224274492005E-5 0.0 0.0 0.0 0.0 9 3.454224274492005E-5 0.0 0.0 0.0 0.0 10-11 3.454224274492005E-5 0.0 0.0 0.0 0.0 12-13 6.90844854898401E-5 0.0 0.0 1.7271121372460023E-5 0.0 14-15 1.0362672823476013E-4 0.0 0.0 3.454224274492005E-5 0.0 16-17 1.0362672823476013E-4 0.0 0.0 6.90844854898401E-5 0.0 18-19 1.554400923521402E-4 0.0 0.0 1.0362672823476013E-4 0.0 20-21 2.0725345646952027E-4 0.0 0.0 1.381689709796802E-4 0.0 22-23 2.0725345646952027E-4 0.0 0.0 2.245245778419803E-4 0.0 24-25 3.108801847042804E-4 0.0 0.0 4.1450691293904053E-4 0.0 26-27 3.108801847042804E-4 0.0 0.0 5.181336411738006E-4 0.0 28-29 3.108801847042804E-4 0.0 0.0 6.73573733525941E-4 0.0 30-31 3.108801847042804E-4 0.0 0.0 0.001468045316659102 0.0 32-33 3.108801847042804E-4 0.0 0.0 0.0034542242744920043 0.0 34-35 3.108801847042804E-4 0.0 0.0 0.006683923971142029 0.0 36-37 3.108801847042804E-4 0.0 0.0 0.011865260382880036 0.0 38-39 3.108801847042804E-4 0.0 0.0 0.020880785739304165 0.0 40-41 3.108801847042804E-4 0.0 0.0 0.03221064135963794 0.0 42-43 3.6269354882166046E-4 0.0 0.0 0.042918736610563155 0.0 44-45 4.1450691293904053E-4 0.0 0.0 0.056062059975005234 0.0 46-47 4.835913984288806E-4 0.0 0.0 0.07176150930257139 1.7271121372460023E-5 48-49 4.835913984288806E-4 0.0 0.0 0.08961984880169506 3.454224274492005E-5 50-51 5.008625198013406E-4 0.0 0.0 0.10979251856472837 3.454224274492005E-5 52-53 5.181336411738006E-4 3.454224274492005E-5 0.0 0.13036242411932825 3.454224274492005E-5 54-55 5.181336411738006E-4 3.454224274492005E-5 0.0 0.1513813788296121 3.454224274492005E-5 56-57 5.181336411738006E-4 3.454224274492005E-5 0.0 0.1774780432233992 3.454224274492005E-5 58-59 5.181336411738006E-4 3.454224274492005E-5 0.0 0.20704620301305077 3.454224274492005E-5 60-61 5.181336411738006E-4 3.454224274492005E-5 0.0 0.24010312931993924 3.454224274492005E-5 62-63 5.181336411738006E-4 3.454224274492005E-5 0.0 0.2739199849672159 3.454224274492005E-5 64-65 5.181336411738006E-4 3.454224274492005E-5 0.0 0.3110874381607499 3.454224274492005E-5 66-67 5.526758839187207E-4 3.454224274492005E-5 0.0 0.3550424420536607 3.454224274492005E-5 68-69 5.526758839187207E-4 3.454224274492005E-5 0.0 0.40673490832143355 3.454224274492005E-5 70-71 5.526758839187207E-4 3.454224274492005E-5 0.0 0.46600939687171633 3.454224274492005E-5 72-73 5.526758839187207E-4 3.454224274492005E-5 0.0 0.5297916481002112 3.454224274492005E-5 74-75 5.526758839187207E-4 3.454224274492005E-5 0.0 0.5936257126928235 3.454224274492005E-5 76-77 5.526758839187207E-4 3.454224274492005E-5 0.0 0.6645754792908892 3.454224274492005E-5 78-79 5.526758839187207E-4 3.454224274492005E-5 0.0 0.7502402412982909 3.454224274492005E-5 80-81 6.73573733525941E-4 3.454224274492005E-5 0.0 0.8498600693746403 3.454224274492005E-5 82-83 6.908448548984009E-4 3.454224274492005E-5 0.0 0.9581154581372198 3.454224274492005E-5 84-85 7.772004617607011E-4 3.454224274492005E-5 0.0 1.0661117800792121 3.454224274492005E-5 86-87 7.94471583133161E-4 3.454224274492005E-5 0.0 1.1809647372060714 3.454224274492005E-5 88-89 7.94471583133161E-4 3.454224274492005E-5 0.0 1.3178211029614446 3.454224274492005E-5 90-91 8.635560686230011E-4 3.454224274492005E-5 0.0 1.4743320048386774 3.454224274492005E-5 92-93 8.635560686230011E-4 3.454224274492005E-5 0.0 1.6442280257795665 3.454224274492005E-5 94-95 8.635560686230011E-4 3.454224274492005E-5 0.0 1.8159029722218194 3.454224274492005E-5 96-97 8.635560686230011E-4 3.454224274492005E-5 1.7271121372460023E-5 1.9947454340336428 3.454224274492005E-5 98-99 8.808271899954611E-4 3.454224274492005E-5 3.454224274492005E-5 2.1953667798961383 3.454224274492005E-5 100-101 9.326405541128412E-4 3.454224274492005E-5 3.454224274492005E-5 2.4261435036749495 3.454224274492005E-5 102-103 0.0010017250396026811 3.454224274492005E-5 3.454224274492005E-5 2.6803225969134434 3.454224274492005E-5 104-105 0.0011398940105823614 3.454224274492005E-5 3.454224274492005E-5 2.9404774981468087 3.454224274492005E-5 106-107 0.0011571651319548216 3.454224274492005E-5 3.454224274492005E-5 3.2042593348683903 3.454224274492005E-5 108-109 0.0011744362533272815 3.454224274492005E-5 3.454224274492005E-5 3.4828425226061706 3.454224274492005E-5 110-111 0.0011744362533272815 3.454224274492005E-5 3.454224274492005E-5 3.8062442703054846 3.454224274492005E-5 112-113 0.0011744362533272815 3.454224274492005E-5 3.454224274492005E-5 4.163601042623055 3.454224274492005E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10285 0.0 41.474842 1 GGTATCA 6725 0.0 40.92849 1 ATCAACG 12485 0.0 33.35857 3 TCAACGC 12610 0.0 33.029602 4 CAACGCA 12665 0.0 32.638687 5 TATCAAC 12980 0.0 32.292088 2 AACGCAG 13150 0.0 31.789566 6 GTACATG 10145 0.0 30.12599 1 TACATGG 10490 0.0 28.605738 2 ACGCAGA 15300 0.0 26.855818 7 ACATGGG 10890 0.0 26.60719 3 CGCAGAG 15460 0.0 26.53936 8 CATGGGG 5605 0.0 24.097427 4 GCAGAGT 16900 0.0 23.644169 9 GTACCGT 230 2.3476059E-7 23.301716 6 GAGTACT 9245 0.0 23.001024 12-13 AGAGTAC 14870 0.0 22.000055 10-11 AGTACTT 9815 0.0 21.999031 12-13 GTACTTT 9760 0.0 21.878887 14-15 GTATACG 235 7.4002273E-6 20.255482 4 >>END_MODULE