FastQCFastQC Report
Thu 26 May 2016
SRR1364626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472614
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47630.19263014768985373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG158800.035.8612061
TACATGG159350.034.7274062
AACGCGG1659.322321E-932.493295
ACATGGG162650.031.715573
GTATCAA114150.031.5716291
CAACGCG1454.033667E-628.756684
TCAACGC128400.027.3713154
ATCAACG130000.027.3551863
CATGGGA116150.027.1297134
CAACGCA131600.026.8431785
TATCAAC137400.026.4455012
AACGCAG132300.026.139616
CGCATAG2757.4578566E-1125.9614498
ATGGGAG42150.025.1569255
CGCAGAT6900.025.005148
CATGGGG50800.024.6243234
GTGTAGC18000.022.8346391
ACGCAGA149450.022.8158077
GGTATCA84200.022.4266661
CGCAGAG144700.022.0382258
Produced by FastQC (version 0.11.5)