FastQCFastQC Report
Thu 26 May 2016
SRR1364626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472614
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116470.4710399601393505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103910.4202435155669263No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66270.26801595396612654No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29090.11764877170476265No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27540.11138010219144598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120100.038.989591
GTATCAA174750.037.4670561
GTACATG161700.031.132631
ATCAACG216850.030.0991173
TCAACGC216900.030.0391394
TACATGG162050.029.983452
TATCAAC222150.029.3500142
CAACGCA219350.029.166415
AACGCAG223750.028.585916
ACATGGG165500.027.9337733
GAGTACT143700.025.31722812-13
AGTACTT145950.024.86573412-13
CGCAGAG255600.024.7447628
ACGCAGA257350.024.6227787
AACGCCG3900.024.4324046
GTACTTT152550.023.96557614-15
CATGGGA119400.023.2227954
GCAGAGT268850.022.904999
CGCGGAA3300.022.5318782-83
TACCTGG23350.022.1856862
Produced by FastQC (version 0.11.5)