##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364626_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2472614 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.612446584869293 31.0 30.0 33.0 26.0 34.0 2 29.68965839391025 31.0 30.0 34.0 26.0 34.0 3 29.793975121066207 31.0 30.0 34.0 26.0 34.0 4 33.48773241597758 35.0 33.0 37.0 28.0 37.0 5 32.85256898165262 35.0 33.0 37.0 26.0 37.0 6 32.87290211897207 35.0 33.0 37.0 26.0 37.0 7 32.67526229326535 35.0 33.0 37.0 25.0 37.0 8 32.615702248713305 35.0 33.0 37.0 25.0 37.0 9 33.89191196037877 38.0 34.0 39.0 23.0 39.0 10-11 33.693186441555376 38.0 33.0 39.0 22.5 39.0 12-13 33.55765639117145 37.0 33.0 39.0 21.5 39.0 14-15 34.52915517747614 38.5 33.0 41.0 21.5 41.0 16-17 34.44877041058572 38.0 33.0 41.0 20.5 41.0 18-19 34.522131032178905 39.0 33.5 41.0 20.0 41.0 20-21 34.55773222185104 39.0 34.0 41.0 21.0 41.0 22-23 34.54766938956101 39.0 34.0 41.0 21.5 41.0 24-25 34.53797620655711 39.0 34.0 41.0 22.0 41.0 26-27 34.42991445490481 39.0 33.5 41.0 21.5 41.0 28-29 34.18024992983135 39.0 33.0 41.0 18.5 41.0 30-31 34.0850822247225 38.0 33.0 41.0 18.5 41.0 32-33 33.864489766700345 38.0 33.0 41.0 17.5 41.0 34-35 33.70197086969499 38.0 33.0 40.0 16.0 41.0 36-37 33.51131333075037 38.0 33.0 40.0 15.5 41.0 38-39 33.360098260383545 38.0 32.5 40.0 14.0 41.0 40-41 33.15282025419253 38.0 32.0 40.0 9.0 41.0 42-43 32.94235351737069 38.0 32.0 40.0 8.5 41.0 44-45 32.68454599059942 38.0 31.5 40.0 4.5 41.0 46-47 32.34108538574965 38.0 31.0 40.0 2.0 41.0 48-49 32.00964080928119 38.0 30.5 40.0 2.0 41.0 50-51 31.787544275006127 38.0 30.0 40.0 2.0 41.0 52-53 31.62131270792772 37.5 30.0 40.0 2.0 41.0 54-55 31.40945675305567 37.0 30.0 40.0 2.0 41.0 56-57 31.189833512226333 37.0 29.0 40.0 2.0 41.0 58-59 31.01386285930598 37.0 29.0 40.0 2.0 41.0 60-61 30.808887476977805 36.0 29.0 40.0 2.0 41.0 62-63 30.55515600089622 36.0 28.5 40.0 2.0 41.0 64-65 30.30572260773416 35.5 28.0 39.0 2.0 41.0 66-67 30.0396620742259 35.0 28.0 39.0 2.0 41.0 68-69 29.73479099446982 35.0 28.0 39.0 2.0 40.5 70-71 29.475107315577766 35.0 28.0 38.0 2.0 40.0 72-73 29.3206972459106 35.0 28.0 37.5 2.0 40.0 74-75 29.03766762624494 35.0 28.0 37.0 2.0 39.5 76-77 28.74048395746364 34.5 28.0 36.5 2.0 39.0 78-79 28.425820002636886 34.0 27.0 36.0 2.0 39.0 80-81 28.14018666075659 34.0 26.5 36.0 2.0 37.5 82-83 27.883094773385576 34.0 26.0 35.0 2.0 37.0 84-85 27.844087067370808 34.0 27.0 35.0 2.0 37.0 86-87 27.681370403953064 34.0 27.0 35.0 2.0 36.0 88-89 27.520801265381493 34.0 26.5 35.0 2.0 36.0 90-91 27.358171150046065 34.0 26.0 35.0 2.0 36.0 92-93 27.21923620104068 34.0 26.0 35.0 2.0 35.5 94-95 27.13123419183099 34.0 26.0 35.0 2.0 35.0 96-97 27.044337490607106 34.0 26.0 35.0 2.0 35.0 98-99 26.94601442036646 34.0 26.0 35.0 2.0 35.0 100-101 26.844013663272957 34.0 25.0 35.0 2.0 35.0 102-103 25.67388905021164 31.5 23.5 34.0 2.0 35.0 104-105 26.58259740501348 33.5 25.0 35.0 2.0 35.0 106-107 26.752107486247347 34.0 25.0 35.0 2.0 35.0 108-109 26.755288937132928 34.0 25.0 35.0 2.0 35.0 110-111 26.699328524387553 34.0 25.0 35.0 2.0 35.0 112-113 26.643929258671186 34.0 25.0 35.0 2.0 35.0 114-115 26.56196337155739 34.0 25.0 35.0 2.0 35.0 116-117 26.44681114804009 34.0 25.0 35.0 2.0 35.0 118-119 26.3412714236836 34.0 25.0 35.0 2.0 35.0 120-121 26.204012838235165 34.0 24.5 35.0 2.0 35.0 122-123 26.021223490605486 34.0 24.0 35.0 2.0 35.0 124-125 25.217714127639816 33.0 20.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5763552002713048 1101 2 0.6263637089206746 1101 3 0.5475020687800694 1101 4 0.5584162894050024 1101 5 0.7785646055246787 1101 6 0.874728381232849 1101 7 0.8159871992159182 1101 8 0.8935063039468147 1101 9 0.9627030976188493 1101 10-11 1.1399954673075285 1101 12-13 1.06971712469025 1101 14-15 1.1866555062909683 1101 16-17 1.31605601088431 1101 18-19 1.2336308208249633 1101 20-21 1.1523091205462137 1101 22-23 1.1713820162337854 1101 24-25 1.094445841535176 1101 26-27 1.2098956270399839 1101 28-29 1.2615638916202911 1101 30-31 1.1868379991388949 1101 32-33 1.3415631479855037 1101 34-35 1.1703730495272993 1101 36-37 1.1212635346926234 1101 38-39 1.2580352045925451 1101 40-41 1.2936039100776 1101 42-43 1.3859970694010784 1101 44-45 1.3224527211647867 1101 46-47 1.4030279664604066 1101 48-49 1.381461840517865 1101 50-51 1.4542294307929033 1101 52-53 1.3884616531981173 1101 54-55 1.368010524393842 1101 56-57 1.3475905398571904 1101 58-59 1.315729128481106 1101 60-61 1.231922049533356 1101 62-63 1.3879135280609454 1101 64-65 1.3334836108681039 1101 66-67 1.2748999581288238 1101 68-69 1.325532070423776 1101 70-71 1.2579356901664234 1101 72-73 1.2360850351550283 1101 74-75 1.194784165639291 1101 76-77 1.2748782460305321 1101 78-79 1.1663120709383499 1101 80-81 1.14929378218212 1101 82-83 0.9939570173727326 1101 84-85 1.0365926607440237 1101 86-87 1.135744678216902 1101 88-89 1.0527332801930704 1101 90-91 1.1315525380204257 1101 92-93 1.036920394809389 1101 94-95 0.9888275701869134 1101 96-97 1.0030966296253538 1101 98-99 1.1122851724496847 1101 100-101 1.1374530294738001 1101 102-103 1.063033671097692 1101 104-105 1.0992163194482814 1101 106-107 1.1463707626094468 1101 108-109 1.1314042629244057 1101 110-111 1.106189030053013 1101 112-113 1.0956762041675532 1101 114-115 1.0503779750685425 1101 116-117 1.1476706270700952 1101 118-119 1.19406608760195 1101 120-121 1.1973106019154933 1101 122-123 1.2105369349253579 1101 124-125 1.0794636275775211 1102 1 0.32275035021790544 1102 2 -0.5522841283950761 1102 3 -0.09182520435185282 1102 4 0.17563741730174343 1102 5 0.6318887764131986 1102 6 0.4340785421992166 1102 7 0.37158212325159923 1102 8 0.4384717383495911 1102 9 0.7916147610928519 1102 10-11 0.789524857177085 1102 12-13 0.7977027436729571 1102 14-15 0.7839793617299051 1102 16-17 0.963825027607335 1102 18-19 0.8741232092330691 1102 20-21 0.8892940031573602 1102 22-23 0.9812492984034336 1102 24-25 0.9773349435908614 1102 26-27 0.9069300945833447 1102 28-29 1.0964900625494494 1102 30-31 0.9329321334217227 1102 32-33 0.8752552111386365 1102 34-35 0.8433560187980547 1102 36-37 0.7448573569125969 1102 38-39 0.6311503691698093 1102 40-41 0.6778802806786075 1102 42-43 0.5876724840414838 1102 44-45 0.37044831003142065 1102 46-47 0.4556935315263928 1102 48-49 0.5904125126544741 1102 50-51 0.5034779479702358 1102 52-53 0.6662929018655994 1102 54-55 0.6422728868402423 1102 56-57 0.44119657365287424 1102 58-59 0.610217679241881 1102 60-61 0.4110505604016943 1102 62-63 0.7177961343711594 1102 64-65 0.7174875917256678 1102 66-67 0.7031957369425434 1102 68-69 0.47348364794583375 1102 70-71 0.345172900185851 1102 72-73 0.4070499979961646 1102 74-75 0.43316341279854953 1102 76-77 0.5731081642620026 1102 78-79 0.5053755340392989 1102 80-81 0.4977760600764256 1102 82-83 0.42170051751481097 1102 84-85 0.5162780143039782 1102 86-87 0.4894528396200002 1102 88-89 0.49211793418390926 1102 90-91 0.46899649975749114 1102 92-93 0.5614500669063283 1102 94-95 0.28616992361494553 1102 96-97 0.19383234642000957 1102 98-99 0.20805008445763562 1102 100-101 0.09245792087754268 1102 102-103 0.05297170041515287 1102 104-105 0.1480493172804529 1102 106-107 0.3615451936471068 1102 108-109 0.3375571746885129 1102 110-111 0.32532536910260745 1102 112-113 0.3652135963929801 1102 114-115 0.468602751923445 1102 116-117 0.4625059981926505 1102 118-119 0.3774296219532154 1102 120-121 0.4329471633602644 1102 122-123 0.3230801842559927 1102 124-125 0.22250348098700812 1103 1 0.331333031353374 1103 2 -0.8542358941489105 1103 3 0.1824223363330013 1103 4 0.4050496433712283 1103 5 0.7475191819880536 1103 6 0.7830200258061453 1103 7 0.7953780384041949 1103 8 0.8939667930261166 1103 9 0.9811226607910868 1103 10-11 1.1504599818754286 1103 12-13 1.227384834493698 1103 14-15 1.1561083207651066 1103 16-17 1.2465312579602426 1103 18-19 1.3433383935000407 1103 20-21 1.3460800520493805 1103 22-23 1.202792638735211 1103 24-25 1.183285677208417 1103 26-27 1.2400213493374537 1103 28-29 1.2738041223085617 1103 30-31 1.1317856119220053 1103 32-33 1.187266186319583 1103 34-35 1.2024222913723364 1103 36-37 1.2116827601196647 1103 38-39 1.3260027542383739 1103 40-41 1.2986687312977097 1103 42-43 1.2285628762433802 1103 44-45 1.3798862439397652 1103 46-47 1.3789217222923327 1103 48-49 1.2228100248501264 1103 50-51 1.220701771600158 1103 52-53 1.2963846670855723 1103 54-55 1.2299952434986956 1103 56-57 1.1580542723119187 1103 58-59 1.2519976793275625 1103 60-61 1.164489449332688 1103 62-63 1.243777413554465 1103 64-65 1.2469789416524968 1103 66-67 1.208687693892049 1103 68-69 1.0240380586971867 1103 70-71 1.0854466964140705 1103 72-73 1.1080635643928112 1103 74-75 1.0458715742876734 1103 76-77 0.9670555022434648 1103 78-79 1.0213814731070094 1103 80-81 1.0679520175624333 1103 82-83 1.0599868417420382 1103 84-85 1.0662665131685287 1103 86-87 1.0178334566806342 1103 88-89 1.049370273379747 1103 90-91 1.073320860777141 1103 92-93 1.0471173947509733 1103 94-95 1.0071251077435122 1103 96-97 1.072288824838246 1103 98-99 1.104876166528289 1103 100-101 1.0455146689298473 1103 102-103 1.0870442261139317 1103 104-105 1.161969728104964 1103 106-107 1.0970376976420226 1103 108-109 1.1948067457027527 1103 110-111 1.138293179434715 1103 112-113 1.1115611620244223 1103 114-115 1.0188783672424293 1103 116-117 1.0688857340488092 1103 118-119 1.1435920950993825 1103 120-121 1.1425369023701712 1103 122-123 1.1394900142006676 1103 124-125 1.0783953248365208 1104 1 0.10225473525592932 1104 2 -0.8880439929101982 1104 3 -0.08770068927242747 1104 4 0.17071563729249561 1104 5 0.48854241909028673 1104 6 0.49062577500866666 1104 7 0.540409080676973 1104 8 0.5618777343902863 1104 9 0.5674425363337221 1104 10-11 0.6657126233433388 1104 12-13 0.5957075748362968 1104 14-15 0.6644364678410639 1104 16-17 0.827052748284018 1104 18-19 0.7335140418676289 1104 20-21 0.8197052507580409 1104 22-23 0.7096601990716991 1104 24-25 0.7935462830380118 1104 26-27 0.6920811930354489 1104 28-29 0.9419236617240614 1104 30-31 0.8919937038928438 1104 32-33 0.8386770618612616 1104 34-35 0.7848638446807072 1104 36-37 0.7044312352318727 1104 38-39 0.7864699402034177 1104 40-41 0.7517423375023782 1104 42-43 0.6919272929551852 1104 44-45 0.7297924588964975 1104 46-47 0.7009770144689398 1104 48-49 0.7702094277392106 1104 50-51 0.7604557556227398 1104 52-53 0.5604018956030181 1104 54-55 0.7596185690689232 1104 56-57 0.717350730741412 1104 58-59 0.6681206831523134 1104 60-61 0.591148856840551 1104 62-63 0.6359166862655492 1104 64-65 0.6821372984027256 1104 66-67 0.6322423135901722 1104 68-69 0.5768530833942336 1104 70-71 0.6908596596979315 1104 72-73 0.6494703176205938 1104 74-75 0.7255576154025505 1104 76-77 0.6801091438929916 1104 78-79 0.6981162321566394 1104 80-81 0.6646422991834129 1104 82-83 0.6433608803892916 1104 84-85 0.6293363141603798 1104 86-87 0.538705753674293 1104 88-89 0.5433562434191401 1104 90-91 0.5328899940950826 1104 92-93 0.6131318314977925 1104 94-95 0.5495875833163169 1104 96-97 0.4797538484302457 1104 98-99 0.5536637033672669 1104 100-101 0.6004381755039283 1104 102-103 0.6259931257572049 1104 104-105 0.5829041801110755 1104 106-107 0.6173054206289308 1104 108-109 0.5376475324593315 1104 110-111 0.49435483861640606 1104 112-113 0.5321210984504638 1104 114-115 0.5776614582603656 1104 116-117 0.5976261413411095 1104 118-119 0.4554044622332363 1104 120-121 0.3878166607840008 1104 122-123 0.47996871830148535 1104 124-125 0.45317996036087393 1105 1 0.507518997096529 1105 2 0.2795326531310778 1105 3 0.4496452472828416 1105 4 0.34614300233451445 1105 5 0.6382877555789861 1105 6 0.41274798613543595 1105 7 0.6103796093222442 1105 8 0.633113296998296 1105 9 0.6277416272663032 1105 10-11 0.815803362893476 1105 12-13 0.8401241286484904 1105 14-15 0.8938846437025205 1105 16-17 0.8603195848783471 1105 18-19 0.8958404093825649 1105 20-21 1.0039650546572574 1105 22-23 0.8197559999736228 1105 24-25 0.8891359153224414 1105 26-27 0.9210280214829822 1105 28-29 0.9456142687366906 1105 30-31 0.9169295250401817 1105 32-33 0.9631683808069766 1105 34-35 0.9297066615889662 1105 36-37 0.9231941071006347 1105 38-39 0.8129899036644019 1105 40-41 0.7504055630577966 1105 42-43 0.7609274251886973 1105 44-45 0.7391593035464297 1105 46-47 0.946513373365903 1105 48-49 0.9753038379093297 1105 50-51 0.9296507423005984 1105 52-53 0.8024282906286082 1105 54-55 0.7301803487192551 1105 56-57 0.6680679075710607 1105 58-59 0.6720442187421263 1105 60-61 0.7325021528749183 1105 62-63 0.7696552317272811 1105 64-65 0.767370386572086 1105 66-67 0.7978069582999971 1105 68-69 0.7651526211849031 1105 70-71 0.7232841536007513 1105 72-73 0.6413520917383941 1105 74-75 0.7477049052994538 1105 76-77 0.655998870260813 1105 78-79 0.6411917557329652 1105 80-81 0.532535089720966 1105 82-83 0.6373014695428019 1105 84-85 0.4514735725492294 1105 86-87 0.5440231369098179 1105 88-89 0.4923248220000467 1105 90-91 0.5089019224701126 1105 92-93 0.3973486215064135 1105 94-95 0.4726788989182005 1105 96-97 0.5644543547174159 1105 98-99 0.5648810572682308 1105 100-101 0.5654890854073074 1105 102-103 0.24850109396644626 1105 104-105 0.2594452705359984 1105 106-107 0.521087679146973 1105 108-109 0.5918308233107474 1105 110-111 0.479759070392884 1105 112-113 0.31472319564361584 1105 114-115 0.44507524428829726 1105 116-117 0.24327512671486673 1105 118-119 0.09500429625737539 1105 120-121 0.07448775043376443 1105 122-123 0.18937842484947964 1105 124-125 0.15676006879235516 1106 1 0.41132155667267867 1106 2 0.43403387664697135 1106 3 0.5424141250936039 1106 4 0.3319998785232272 1106 5 0.6530636108463526 1106 6 0.6676442168543133 1106 7 0.7291972911287701 1106 8 0.6527050518749107 1106 9 0.7964974170199142 1106 10-11 0.901335057868863 1106 12-13 0.7395306261019172 1106 14-15 0.8538840486572639 1106 16-17 0.9512260077674668 1106 18-19 0.9760683411007491 1106 20-21 1.0535980733512318 1106 22-23 0.9190778687683476 1106 24-25 0.9605538347063032 1106 26-27 0.8655592249950743 1106 28-29 1.1572314988965289 1106 30-31 1.0225215179348979 1106 32-33 0.7999391289698679 1106 34-35 0.8392098281847424 1106 36-37 1.0897636197732652 1106 38-39 1.153248278191768 1106 40-41 1.027562951058897 1106 42-43 1.0164832745937673 1106 44-45 1.0068374002243843 1106 46-47 0.8343783803705378 1106 48-49 0.9998452673843516 1106 50-51 1.0769175745514623 1106 52-53 1.0959948963949522 1106 54-55 1.0586415469934742 1106 56-57 0.7771514570674434 1106 58-59 0.8156446539332514 1106 60-61 0.9550785004871329 1106 62-63 0.9512414828025868 1106 64-65 0.9410286880778429 1106 66-67 0.8896692001807516 1106 68-69 0.9370258971147365 1106 70-71 0.9686733976802344 1106 72-73 1.0257379940143316 1106 74-75 0.9437804716392861 1106 76-77 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-3.0012106798189038 2216 74-75 -2.6427875125199947 2216 76-77 -2.927998507838108 2216 78-79 -2.490961904444589 2216 80-81 -2.08597210409733 2216 82-83 -2.03318002064999 2216 84-85 -1.9901646051018886 2216 86-87 -2.080037090236523 2216 88-89 -1.9357015319347184 2216 90-91 -1.876282057313169 2216 92-93 -1.911626649116446 2216 94-95 -1.7638346898104231 2216 96-97 -1.8618440302668802 2216 98-99 -1.8286600346800306 2216 100-101 -1.9953949948396428 2216 102-103 -1.880728362490487 2216 104-105 -1.794061932398776 2216 106-107 -1.9506892027842113 2216 108-109 -2.0679405595567033 2216 110-111 -1.8985403526551714 2216 112-113 -1.9819543621837639 2216 114-115 -2.0242846143697193 2216 116-117 -2.150479959524045 2216 118-119 -2.1610052906382258 2216 120-121 -2.1970995389982235 2216 122-123 -2.1198301974004607 2216 124-125 -2.1773469806208148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93750.0 3 30677.0 4 13954.0 5 11042.0 6 12114.0 7 14472.0 8 16564.0 9 17468.0 10 19298.0 11 22002.0 12 25825.0 13 29952.0 14 34710.0 15 28677.0 16 17107.0 17 14221.0 18 12805.0 19 11679.0 20 11294.0 21 11657.0 22 13248.0 23 17224.0 24 23854.0 25 31691.0 26 38006.0 27 41574.0 28 48050.0 29 55983.0 30 65647.0 31 76677.0 32 93553.0 33 116460.0 34 149787.0 35 205913.0 36 317976.0 37 505660.0 38 220824.0 39 1219.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.51010048689526 19.95416742204496 13.736176577229879 23.799555513829898 2 15.162387807436764 21.17687246635842 38.983515949799894 24.677223776404915 3 18.924708830411863 25.802935678597628 29.80562271345224 25.466732777538265 4 12.266926059022547 17.668906689532022 36.68105862820002 33.38310862324541 5 12.545268748300906 39.36799325540151 33.59713807655961 14.489599919737984 6 30.89932718704653 37.7233070883975 17.29189146341266 14.085474261143313 7 27.39272565941481 34.01184740490712 20.368889703143523 18.226537232534543 8 26.188823802521384 36.17223405855832 19.074970112577308 18.56397202634299 9 26.628854855382766 16.072338546208115 19.897508529496932 37.40129806891218 10-11 24.572229372669177 27.771558091721634 26.63572628588159 21.0204862497276 12-13 25.82923853420854 25.04554888914101 27.81423778694182 21.31097478970863 14-15 22.552868951171337 26.025937035941237 26.80697643049162 24.614217582395803 16-17 21.21963078119635 29.384359481796324 27.444403820749553 21.951605916257773 18-19 20.97528522492427 27.11703422699091 30.928937438577048 20.978743109507768 20-21 22.22455021579894 26.083024295556672 31.013647278098 20.67877821054639 22-23 21.489717993107575 26.288790104680697 30.814713543773358 21.406778358438363 24-25 21.226382282070716 26.325196735115146 31.21356588614317 21.234855096670973 26-27 21.154959892648023 26.64384736153722 31.022897225365544 21.178295520449208 28-29 20.805263075899305 26.477645260737003 31.309078041132548 21.408013622231138 30-31 21.048513840008994 26.395830485470036 31.375722211392475 21.179933463128496 32-33 20.9161681696232 26.42976353434387 31.130098072185998 21.523970223846927 34-35 21.296510494561627 26.412816557699664 31.093106323914693 21.19756662382402 36-37 21.406860721953286 26.241971292295613 30.92422727953825 21.426940706212843 38-39 21.290100274446395 26.396518016965043 30.964457048289784 21.348924660298778 40-41 21.4820631121558 26.26855627283515 30.941910059556406 21.30747055545265 42-43 21.380254654963636 26.383223283756426 30.590607484989015 21.645914576290927 44-45 21.530817183757755 26.6992138684 30.10370805956773 21.666260888274515 46-47 21.534121999682117 26.94184249617712 29.433700300046954 22.090335204093805 48-49 21.90149275461961 26.96084299586267 29.144891430512697 21.99277281900502 50-51 21.92093397872567 27.281819635934497 28.75144407445671 22.04580231088312 52-53 22.006813444004447 27.389482739201043 28.604695885301833 21.999007931492677 54-55 21.705955721354 27.247540457184176 28.759765980456308 22.28673784100551 56-57 21.847922294663675 27.577301606849385 28.47399963964642 22.100776458840524 58-59 21.89003216838536 27.14538136563168 28.766317751173453 22.19826871480951 60-61 21.952617755945735 27.16926297432596 28.9825261848392 21.895593084889107 62-63 21.8577384096345 27.08975197907963 28.992616720604186 22.059892890681684 64-65 21.6749558410622 27.021751373299995 29.1007753975821 22.202517388055703 66-67 21.72652100166059 27.337950848777854 28.79986524382698 22.13566290573458 68-69 21.910094637223978 27.10915635363585 28.83319178192995 22.147557227210225 70-71 21.694692337744588 27.110074601211508 28.80710454603913 22.38812851500477 72-73 21.87255630940563 27.125236065440504 28.740480791922078 22.261726833231783 74-75 21.773635483791384 27.23891960625154 28.605731022675844 22.381713887281233 76-77 21.83573970719081 27.13665776914988 28.715603008978402 22.311999514680902 78-79 21.847822724006928 27.1536621523164 28.65123767666904 22.34727744700763 80-81 21.601328171368078 27.54608813290611 28.58302436025637 22.269559335469435 82-83 21.789569087264805 27.47123285011963 28.40716618701195 22.332031875603615 84-85 21.753761154957015 27.15408385021262 28.428384321596823 22.663770673233543 86-87 21.744549823697977 27.115952881244404 28.48222531432961 22.657271980728005 88-89 21.90087090018903 26.974084915801654 28.4963403102951 22.628703873714215 90-91 21.682862751727523 27.348021163028275 28.57115991416371 22.397956171080484 92-93 21.57919057610152 27.246411688869788 28.657185469804595 22.517212265224096 94-95 21.421987532371304 27.26589020025618 28.523977717945886 22.78814454942663 96-97 21.696165910572223 27.046495510855667 28.450155704965635 22.807182873606475 98-99 21.638179360327268 27.02467147336247 28.25004524603225 23.087103920278008 100-101 21.424990763791474 27.157223422822607 28.476176244047018 22.9416095693389 102-103 21.74677593031662 26.865592566566736 28.478764869141443 22.908866633975197 104-105 21.533870930560337 26.808485942165493 28.49917111777165 23.15847200950252 106-107 21.45968060437284 26.995565443828717 28.494141690227377 23.050612261571068 108-109 21.516264235022614 27.00394457294629 28.11403063525299 23.36576055677811 110-111 21.2719516426878 27.052560994647727 28.319732232626947 23.355755130037522 112-113 21.481592478651976 26.864427224604054 28.221960817144993 23.432019479598978 114-115 21.10173594105331 26.93517408953384 28.234470742823266 23.728619226589586 116-117 21.129933700321246 26.900873224159085 28.377884306999906 23.591308768519763 118-119 21.10711683588692 26.968536538855474 28.211212923899115 23.713133701358487 120-121 20.9621196677442 27.002862098311216 28.211710334294377 23.823307899650207 122-123 20.986854690602133 26.903800989487554 28.120620741060094 23.98872357885022 124-125 20.90376777855924 26.880522338893403 28.149808438261225 24.06590144428613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 330.0 1 233.0 2 336.5 3 632.5 4 526.0 5 469.0 6 1192.0 7 2661.5 8 4047.5 9 4609.5 10 3855.0 11 2733.0 12 2572.5 13 2836.0 14 3155.5 15 3590.5 16 4055.0 17 4470.5 18 4880.0 19 5286.0 20 5862.5 21 6625.5 22 7660.5 23 8991.0 24 10605.5 25 12731.0 26 15525.5 27 19137.5 28 22980.5 29 27232.5 30 32170.5 31 36710.0 32 41667.0 33 47503.5 34 53923.0 35 59682.0 36 64708.0 37 70186.0 38 74418.0 39 76208.0 40 76768.5 41 79609.5 42 83363.5 43 85808.5 44 87562.5 45 88876.5 46 90061.0 47 92022.0 48 98709.5 49 100469.5 50 95419.5 51 89179.5 52 82219.5 53 78042.5 54 73712.5 55 67135.0 56 59267.0 57 51796.0 58 45830.5 59 40282.5 60 34264.5 61 28258.0 62 22724.5 63 17556.0 64 13405.0 65 10769.5 66 8212.0 67 6295.5 68 4554.5 69 2948.5 70 2215.0 71 1793.5 72 1543.5 73 1412.5 74 1084.0 75 840.0 76 624.0 77 348.5 78 219.5 79 160.0 80 120.0 81 85.5 82 55.5 83 45.5 84 37.0 85 24.0 86 14.5 87 11.0 88 6.5 89 3.0 90 3.0 91 3.0 92 2.0 93 1.0 94 1.5 95 2.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05848062010487687 2 0.07530492021803646 3 0.0 4 0.006834871920971086 5 0.028714550673902193 6 4.0443029118172104E-4 7 2.0221514559086052E-4 8 8.088605823634421E-5 9 7.279745241270979E-4 10-11 0.06137229668682617 12-13 0.010939839376465553 14-15 0.008230156425548023 16-17 0.05757065194971799 18-19 1.6177211647268842E-4 20-21 0.011708256929710824 22-23 0.013507971725469482 24-25 0.0 26-27 0.0 28-29 0.05498229808615498 30-31 0.0 32-33 0.003983638368139952 34-35 0.0 36-37 1.0110757279543026E-4 38-39 0.0 40-41 0.0 42-43 0.0037207586788718335 44-45 0.0 46-47 4.0443029118172106E-5 48-49 1.6177211647268842E-4 50-51 3.84208776622635E-4 52-53 1.0110757279543026E-4 54-55 0.0 56-57 0.0017188287375223144 58-59 0.0 60-61 0.0 62-63 0.0 64-65 4.650948348589792E-4 66-67 0.0 68-69 5.662024076544095E-4 70-71 0.0 72-73 0.001921043883113175 74-75 0.0028714550673902195 76-77 5.662024076544095E-4 78-79 7.077530095680118E-4 80-81 0.0016783857084041423 82-83 8.088605823634421E-5 84-85 1.0110757279543026E-4 86-87 4.2465180574080715E-4 88-89 0.0 90-91 0.0 92-93 0.0071381946393573766 94-95 0.005298036814480545 96-97 0.0060462328531667294 98-99 8.695251260407002E-4 100-101 3.437657475044629E-4 102-103 6.066454367725816E-5 104-105 2.8310120382720475E-4 106-107 0.004064524426376296 108-109 0.004165631999171727 110-111 8.088605823634421E-4 112-113 0.0031747777857765104 114-115 2.8310120382720475E-4 116-117 0.0021434805432631217 118-119 0.001921043883113175 120-121 0.012213794793687976 122-123 8.493036114816143E-4 124-125 0.0017390502520814005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2472614.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.16956658864384 #Duplication Level Percentage of deduplicated Percentage of total 1 76.57391995854836 43.01123896065165 2 12.491230372866793 14.032539924056636 3 4.647782428933442 7.831917738945172 4 2.1728195696616552 4.8818533401287505 5 1.2075018445210781 3.3912427630868476 6 0.7181241822355034 2.420203444379551 7 0.48674264336946566 1.9138086322791605 8 0.30152093642210365 1.3549040252985276 9 0.22857449616358383 1.1555037344453523 >10 1.0575866424967957 10.228082772435759 >50 0.06927906954074127 2.700788333143372 >100 0.04136206517299133 4.26828469880731 >500 0.002539850048314872 0.9652122015660484 >1k 7.982385866110461E-4 0.6760153869484418 >5k 7.256714423736783E-5 0.2701208302265495 >10k+ 1.4513428847473566E-4 0.8982832136008296 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11647 0.4710399601393505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10391 0.4202435155669263 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6627 0.26801595396612654 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2909 0.11764877170476265 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2754 0.11138010219144598 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 8.088605823634421E-5 0.0 0.0 0.0 3 0.0 8.088605823634421E-5 0.0 0.0 0.0 4 0.0 8.088605823634421E-5 0.0 0.0 0.0 5 0.0 8.088605823634421E-5 0.0 0.0 0.0 6 0.0 8.088605823634421E-5 0.0 0.0 0.0 7 0.0 8.088605823634421E-5 0.0 0.0 0.0 8 0.0 8.088605823634421E-5 0.0 0.0 0.0 9 0.0 8.088605823634421E-5 0.0 0.0 0.0 10-11 0.0 8.088605823634421E-5 0.0 4.0443029118172106E-5 0.0 12-13 0.0 8.088605823634421E-5 0.0 4.0443029118172106E-5 0.0 14-15 0.0 8.088605823634421E-5 0.0 4.0443029118172106E-5 0.0 16-17 0.0 8.088605823634421E-5 0.0 1.0110757279543026E-4 0.0 18-19 0.0 8.088605823634421E-5 0.0 1.2132908735451631E-4 0.0 20-21 0.0 8.088605823634421E-5 0.0 1.4155060191360237E-4 0.0 22-23 0.0 8.088605823634421E-5 0.0 2.2243666014994657E-4 0.0 24-25 0.0 1.0110757279543026E-4 0.0 6.875314950089258E-4 0.0 26-27 0.0 1.2132908735451631E-4 0.0 0.001172847844426991 0.0 28-29 0.0 1.2132908735451631E-4 0.0 0.0020221514559086054 0.0 30-31 0.0 1.2132908735451631E-4 0.0 0.005399144387275976 0.0 32-33 0.0 1.2132908735451631E-4 0.0 0.01146559875500179 0.0 34-35 0.0 1.2132908735451631E-4 0.0 0.01931154640392718 0.0 36-37 0.0 1.2132908735451631E-4 0.0 0.03344638508072833 0.0 38-39 0.0 1.4155060191360237E-4 0.0 0.055508057464691216 0.0 40-41 0.0 1.6177211647268842E-4 0.0 0.08319131089608002 0.0 42-43 0.0 2.0221514559086052E-4 0.0 0.10846820409493758 0.0 44-45 0.0 2.0221514559086052E-4 0.0 0.1370209826523671 0.0 46-47 0.0 2.0221514559086052E-4 0.0 0.17117512074266344 0.0 48-49 0.0 2.0221514559086052E-4 0.0 0.21105194745318112 0.0 50-51 0.0 2.0221514559086052E-4 0.0 0.2570558930751019 0.0 52-53 0.0 2.0221514559086052E-4 0.0 0.30059281392081416 0.0 54-55 0.0 2.2243666014994657E-4 0.0 0.3460103356205214 0.0 56-57 0.0 2.4265817470903262E-4 0.0 0.4012757349105036 0.0 58-59 0.0 2.4265817470903262E-4 0.0 0.46616657513061077 0.0 60-61 0.0 3.2354423294537685E-4 0.0 0.5391460211743524 0.0 62-63 0.0 3.2354423294537685E-4 0.0 0.6105886321116033 0.0 64-65 0.0 3.2354423294537685E-4 0.0 0.6794631106998504 0.0 66-67 0.0 3.2354423294537685E-4 0.0 0.7625735355376941 0.0 68-69 0.0 3.2354423294537685E-4 0.0 0.863863101964156 0.0 70-71 0.0 3.2354423294537685E-4 0.0 0.98060190551376 0.0 72-73 0.0 3.2354423294537685E-4 0.0 1.099019094771768 0.0 74-75 0.0 3.2354423294537685E-4 0.0 1.2115113802639637 0.0 76-77 0.0 3.2354423294537685E-4 0.0 1.3366825553847064 0.0 78-79 0.0 3.2354423294537685E-4 0.0 1.4921051162858416 0.0 80-81 0.0 3.2354423294537685E-4 0.0 1.6698117862310897 0.0 82-83 0.0 3.2354423294537685E-4 0.0 1.8603591179213577 0.0 84-85 0.0 3.2354423294537685E-4 0.0 2.044658001612868 0.0 86-87 0.0 3.2354423294537685E-4 0.0 2.239876503166285 0.0 88-89 0.0 3.2354423294537685E-4 0.0 2.4662765801698123 0.0 90-91 0.0 3.2354423294537685E-4 0.0 2.7245255426039003 0.0 92-93 0.0 3.2354423294537685E-4 0.0 3.004168867441501 0.0 94-95 0.0 3.2354423294537685E-4 0.0 3.2717601696018868 0.0 96-97 0.0 3.2354423294537685E-4 0.0 3.545438147644558 0.0 98-99 0.0 3.2354423294537685E-4 0.0 3.8577796615242006 0.0 100-101 0.0 3.2354423294537685E-4 0.0 4.227732270382679 0.0 102-103 0.0 3.2354423294537685E-4 0.0 4.6269454108081565 0.0 104-105 0.0 3.2354423294537685E-4 0.0 5.028382917835133 0.0 106-107 0.0 3.2354423294537685E-4 0.0 5.4256750143774966 0.0 108-109 0.0 3.2354423294537685E-4 0.0 5.845372549051328 0.0 110-111 0.0 3.2354423294537685E-4 0.0 6.326179500722716 0.0 112-113 0.0 3.2354423294537685E-4 0.0 6.853779037083831 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12010 0.0 38.98959 1 GTATCAA 17475 0.0 37.467056 1 GTACATG 16170 0.0 31.13263 1 ATCAACG 21685 0.0 30.099117 3 TCAACGC 21690 0.0 30.039139 4 TACATGG 16205 0.0 29.98345 2 TATCAAC 22215 0.0 29.350014 2 CAACGCA 21935 0.0 29.16641 5 AACGCAG 22375 0.0 28.58591 6 ACATGGG 16550 0.0 27.933773 3 GAGTACT 14370 0.0 25.317228 12-13 AGTACTT 14595 0.0 24.865734 12-13 CGCAGAG 25560 0.0 24.744762 8 ACGCAGA 25735 0.0 24.622778 7 AACGCCG 390 0.0 24.432404 6 GTACTTT 15255 0.0 23.965576 14-15 CATGGGA 11940 0.0 23.222795 4 GCAGAGT 26885 0.0 22.90499 9 CGCGGAA 330 0.0 22.53187 82-83 TACCTGG 2335 0.0 22.185686 2 >>END_MODULE