FastQCFastQC Report
Thu 26 May 2016
SRR1364625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285587
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54150.046.2121351
GGTATCA33200.046.1211621
ATCAACG64850.038.1254273
TCAACGC64900.037.820664
CAACGCA66000.036.9195145
AACGCAG66900.036.0302436
TATCAAC70400.034.273542
GTACATG82100.033.3100051
TACATGG83550.031.232322
ACGCAGA78400.030.1332577
ACATGGG86450.029.3576433
CGCAGAG77450.028.8855698
GTACCTG23650.024.9407371
TACCTGG23100.024.501252
CATGGGA47250.024.0828674
GCAGAGT87300.023.5141269
GAGTACA67050.023.1036051
GTATAGG7800.022.9156321
AGTACAT62700.021.4742242
CAGAGTA86200.020.98133910-11
Produced by FastQC (version 0.11.5)