##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364625_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2285587 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.68987266728416 30.0 26.0 31.0 2.0 34.0 2 25.208910883724837 30.0 24.0 31.0 2.0 34.0 3 25.907075950291983 31.0 25.0 31.0 2.0 34.0 4 29.204636270682325 35.0 28.0 37.0 2.0 37.0 5 29.48538121716653 35.0 30.0 37.0 2.0 37.0 6 29.406172681241188 35.0 30.0 37.0 2.0 37.0 7 29.25456698869918 35.0 29.0 37.0 2.0 37.0 8 29.089723121456327 35.0 28.0 37.0 2.0 37.0 9 30.021591389870522 37.0 28.0 39.0 2.0 39.0 10-11 29.9518217858257 36.0 27.5 39.0 2.0 39.0 12-13 29.924246593982204 37.0 27.0 39.0 2.0 39.0 14-15 30.500090786305663 37.0 27.0 40.0 2.0 41.0 16-17 30.85908980931376 37.0 28.0 40.0 2.0 41.0 18-19 30.669930306743957 37.0 27.5 40.0 2.0 41.0 20-21 30.579879917062883 37.0 27.0 40.0 2.0 41.0 22-23 30.620663313188253 37.0 27.0 40.0 2.0 41.0 24-25 30.702448648859132 37.0 27.5 40.0 2.0 41.0 26-27 30.49964888669738 37.0 27.0 40.0 2.0 41.0 28-29 30.377315761771484 37.0 27.0 40.0 2.0 41.0 30-31 30.222573894583753 37.0 27.0 40.0 2.0 41.0 32-33 30.12738128104509 37.0 27.0 40.0 2.0 41.0 34-35 30.168347999879245 37.0 27.0 40.0 2.0 41.0 36-37 30.087685351728027 37.0 27.0 40.0 2.0 41.0 38-39 29.793479968165727 36.0 26.0 40.0 2.0 41.0 40-41 29.645299872636656 36.0 26.0 40.0 2.0 41.0 42-43 29.119590722208343 35.5 24.5 40.0 2.0 41.0 44-45 28.93503616357636 35.0 24.0 40.0 2.0 41.0 46-47 28.866713890129756 35.0 24.0 40.0 2.0 41.0 48-49 28.785603873315694 35.0 24.0 40.0 2.0 41.0 50-51 28.54704524483207 35.0 24.0 39.5 2.0 41.0 52-53 28.442452420319157 35.0 23.5 39.0 2.0 41.0 54-55 28.262002715276207 35.0 23.0 39.0 2.0 40.5 56-57 28.03429031579196 34.5 22.5 39.0 2.0 40.0 58-59 27.821784294362892 34.0 22.0 39.0 2.0 40.0 60-61 27.526763802909272 34.0 21.0 39.0 2.0 40.0 62-63 27.35006696310401 34.0 20.5 38.0 2.0 40.0 64-65 27.117034486107947 34.0 20.5 38.0 2.0 40.0 66-67 26.86048441822604 33.0 20.0 38.0 2.0 40.0 68-69 26.62167618209239 33.0 19.0 37.0 2.0 40.0 70-71 26.205255805182652 33.0 17.5 36.5 2.0 39.0 72-73 25.983120966298813 32.5 17.5 36.0 2.0 39.0 74-75 25.955509897457414 33.0 18.0 36.0 2.0 39.0 76-77 25.712835914800003 32.5 16.0 35.5 2.0 38.5 78-79 25.43665215981715 32.0 13.0 35.0 2.0 37.5 80-81 25.05230997551176 32.0 10.0 35.0 2.0 37.0 82-83 25.178235394233518 32.0 11.5 35.0 2.0 36.5 84-85 25.030429600798392 32.0 8.0 35.0 2.0 36.0 86-87 24.912670574342606 32.0 7.5 35.0 2.0 36.0 88-89 24.761355616740907 32.0 7.0 35.0 2.0 35.5 90-91 24.637826300202093 32.0 7.0 35.0 2.0 35.0 92-93 24.520275535343874 32.0 2.0 35.0 2.0 35.0 94-95 24.42323810032171 32.0 2.0 35.0 2.0 35.0 96-97 24.314771216322107 32.0 2.0 35.0 2.0 35.0 98-99 24.224734389896337 32.0 2.0 35.0 2.0 35.0 100-101 24.121800001487582 32.0 2.0 35.0 2.0 35.0 102-103 23.01343506066494 30.0 2.0 34.0 2.0 35.0 104-105 23.834683168918968 32.0 2.0 34.5 2.0 35.0 106-107 23.99468736040238 32.0 2.0 35.0 2.0 35.0 108-109 23.954685382792256 32.0 2.0 35.0 2.0 35.0 110-111 23.887737373374982 32.0 2.0 35.0 2.0 35.0 112-113 23.7945790293697 32.0 2.0 35.0 2.0 35.0 114-115 23.69668076516011 32.0 2.0 35.0 2.0 35.0 116-117 23.586186393254778 32.0 2.0 35.0 2.0 35.0 118-119 23.458832895006843 32.0 2.0 35.0 2.0 35.0 120-121 23.271128379711648 32.0 2.0 35.0 2.0 35.0 122-123 23.06283090514603 32.0 2.0 35.0 2.0 35.0 124-125 22.143693939456256 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.316375437599536 1101 2 1.6541373933789636 1101 3 1.250724644909745 1101 4 1.4676222001024009 1101 5 1.2840429349351794 1101 6 1.0432285605723628 1101 7 1.1422458581027648 1101 8 1.400938036018367 1101 9 1.5937190036494435 1101 10-11 1.6072096540505427 1101 12-13 1.5645827907964218 1101 14-15 1.8632808491951103 1101 16-17 1.4711437389450523 1101 18-19 1.5168263822045667 1101 20-21 1.697284910036533 1101 22-23 1.4404601186660138 1101 24-25 1.4593200641961737 1101 26-27 1.7109800084591278 1101 28-29 1.645229513018247 1101 30-31 1.6722341383110688 1101 32-33 1.643678156488324 1101 34-35 1.4821746134191969 1101 36-37 1.3675079648166317 1101 38-39 1.3802089147355368 1101 40-41 1.3456028244886937 1101 42-43 1.6609739798211933 1101 44-45 1.3909507928245581 1101 46-47 1.1992948942469255 1101 48-49 1.0803285931115987 1101 50-51 1.1176826326333291 1101 52-53 0.9968924191725641 1101 54-55 1.0121387084701574 1101 56-57 0.8692092864491947 1101 58-59 0.871757656433882 1101 60-61 0.9663120301765602 1101 62-63 0.8948086748764439 1101 64-65 0.8640294104829138 1101 66-67 0.9001871680908877 1101 68-69 0.8474631943911355 1101 70-71 0.7216456151756034 1101 72-73 0.610088129637397 1101 74-75 0.6322585714735212 1101 76-77 0.7276193475541284 1101 78-79 0.6843798136217387 1101 80-81 0.839942086776194 1101 82-83 0.8358717693130657 1101 84-85 0.8876309489689689 1101 86-87 0.8184178702082185 1101 88-89 0.7854373292003274 1101 90-91 0.7753637132878097 1101 92-93 0.7993955148891452 1101 94-95 0.6436814966022872 1101 96-97 0.6948207552603911 1101 98-99 0.7757983671345166 1101 100-101 0.7747167018525047 1101 102-103 0.5450631798632948 1101 104-105 0.8090898918639908 1101 106-107 0.8941824289566966 1101 108-109 0.862601032724502 1101 110-111 0.8471055521719961 1101 112-113 0.7469399572241748 1101 114-115 0.7988080790977783 1101 116-117 0.7303060977844318 1101 118-119 0.7269801106210139 1101 120-121 0.7030192640935198 1101 122-123 0.577578290989571 1101 124-125 0.5653740903688593 1102 1 1.0827557682832136 1102 2 1.533699505620259 1102 3 1.1848115695404289 1102 4 1.1651995851396748 1102 5 1.0801620833038506 1102 6 0.7163880872462727 1102 7 0.8620805579396738 1102 8 0.8045717500833973 1102 9 1.360063215295888 1102 10-11 1.3199915909200222 1102 12-13 1.520256049650449 1102 14-15 1.7918024900551863 1102 16-17 1.3930085370535252 1102 18-19 1.2347962630950988 1102 20-21 1.6599593580403997 1102 22-23 1.674936225922881 1102 24-25 1.265750155799605 1102 26-27 1.6257760678721702 1102 28-29 1.2395394449199735 1102 30-31 1.270850570805976 1102 32-33 1.622137144710738 1102 34-35 1.494425044763851 1102 36-37 1.2584734475197799 1102 38-39 1.569680007003008 1102 40-41 1.4781912952920138 1102 42-43 1.7146848983283078 1102 44-45 1.2753874122493762 1102 46-47 1.142003502524851 1102 48-49 1.1212377440085994 1102 50-51 1.2066083968982646 1102 52-53 1.0734130306400793 1102 54-55 1.0988587803748295 1102 56-57 1.0990876833751884 1102 58-59 1.0345143794369847 1102 60-61 1.01318881008104 1102 62-63 0.8770278632538897 1102 64-65 0.7819907478319408 1102 66-67 0.8574633828713516 1102 68-69 0.7953363040457582 1102 70-71 0.991570126387785 1102 72-73 0.5956087369373364 1102 74-75 0.8132786772745177 1102 76-77 0.6548476490425124 1102 78-79 0.7954769363236665 1102 80-81 0.9300743658040886 1102 82-83 0.9714442282693376 1102 84-85 0.8491150315870968 1102 86-87 0.8691788358090022 1102 88-89 0.825040290683539 1102 90-91 0.8507035100765492 1102 92-93 0.8813400596642467 1102 94-95 0.79863685486146 1102 96-97 0.6466647015708311 1102 98-99 0.8425286357212336 1102 100-101 0.8744312919986719 1102 102-103 0.706472732237291 1102 104-105 0.722232289779086 1102 106-107 0.6785884166595508 1102 108-109 0.8092535620295394 1102 110-111 0.7255087295359175 1102 112-113 0.6265176978950429 1102 114-115 0.7163580414506292 1102 116-117 0.7513185213440465 1102 118-119 0.8116530319009598 1102 120-121 0.8921171841926494 1102 122-123 0.7862148737396737 1102 124-125 0.6344547011139952 1103 1 1.089616992589697 1103 2 1.3532077893694598 1103 3 1.0967330947972833 1103 4 0.9042192052289479 1103 5 1.0192178081748864 1103 6 0.875046498116582 1103 7 0.9242322731097872 1103 8 1.1652206256422488 1103 9 1.4904633187331058 1103 10-11 1.6940646295952995 1103 12-13 1.7034778172198628 1103 14-15 1.8832755248006912 1103 16-17 1.4759696238881546 1103 18-19 1.5232136017411086 1103 20-21 1.637288432249239 1103 22-23 1.4667102303396682 1103 24-25 1.4130657354515215 1103 26-27 1.6832426262127989 1103 28-29 1.622114607075705 1103 30-31 1.6877764153034889 1103 32-33 1.7835138088113531 1103 34-35 1.6123769369842336 1103 36-37 1.4920444121876208 1103 38-39 1.378029053577123 1103 40-41 1.5926057346784326 1103 42-43 1.7407102092189 1103 44-45 1.3391929211583786 1103 46-47 1.2492629495918735 1103 48-49 1.0957440926958775 1103 50-51 1.1563329268869857 1103 52-53 1.0232936111797066 1103 54-55 1.1418782067824758 1103 56-57 1.1112768629580572 1103 58-59 1.186072139368605 1103 60-61 1.100663564803611 1103 62-63 0.8967442231761105 1103 64-65 0.8274110632803691 1103 66-67 0.9409827517359091 1103 68-69 0.9214819466652564 1103 70-71 1.0026765572494085 1103 72-73 0.940868914170057 1103 74-75 1.0162607515486144 1103 76-77 0.8961321950731254 1103 78-79 0.8897602844755141 1103 80-81 1.029117255758539 1103 82-83 0.937305034335786 1103 84-85 0.9334746524061863 1103 86-87 0.9126120724886562 1103 88-89 0.8930342525184329 1103 90-91 0.9162216715887794 1103 92-93 0.8807169539372381 1103 94-95 0.8941728178907304 1103 96-97 0.9796488582733041 1103 98-99 0.9426463359062822 1103 100-101 0.7836899580234764 1103 102-103 0.8058620649409249 1103 104-105 0.9117990604677573 1103 106-107 0.9831426454101901 1103 108-109 1.0435134210208972 1103 110-111 0.9172206470612281 1103 112-113 1.0021767241957455 1103 114-115 1.0901350753007044 1103 116-117 1.005217109713744 1103 118-119 0.978832951958168 1103 120-121 0.8721675817662984 1103 122-123 0.6579039091139407 1103 124-125 0.6462520438160944 1104 1 1.0537564461935212 1104 2 1.5229905104758288 1104 3 0.8975714874658536 1104 4 1.3184540428184022 1104 5 0.7092129809770533 1104 6 0.8948957500040287 1104 7 0.5331910126835808 1104 8 0.9226592618875458 1104 9 1.1869776144734665 1104 10-11 1.282387184363543 1104 12-13 1.2761996212892512 1104 14-15 1.312550503401372 1104 16-17 1.193751901971524 1104 18-19 1.128220062349957 1104 20-21 1.3543511846181104 1104 22-23 1.209867088835754 1104 24-25 1.261855310010251 1104 26-27 1.5337637797017045 1104 28-29 1.5522588765354222 1104 30-31 1.6359523480189821 1104 32-33 1.630128308861277 1104 34-35 1.4156717615717014 1104 36-37 1.3778584566353018 1104 38-39 1.3258355999722689 1104 40-41 1.296164706143319 1104 42-43 1.493286418883919 1104 44-45 1.2489836950411934 1104 46-47 1.1231921889550662 1104 48-49 0.991717034267193 1104 50-51 0.9834569927451575 1104 52-53 1.0205382934000653 1104 54-55 1.0195021256250598 1104 56-57 0.9432913235861484 1104 58-59 0.8931175110757295 1104 60-61 0.9698981867243788 1104 62-63 0.7847073072279507 1104 64-65 0.7161873299644057 1104 66-67 0.6681001756860638 1104 68-69 0.6140602459965478 1104 70-71 0.5712115104407118 1104 72-73 0.4904195671821796 1104 74-75 0.5322575679008033 1104 76-77 0.6289474824941763 1104 78-79 0.6674013193346191 1104 80-81 0.6008578962103179 1104 82-83 0.5784170100310391 1104 84-85 0.6645350325914094 1104 86-87 0.5339181018804311 1104 88-89 0.5624317343674647 1104 90-91 0.5358614241214283 1104 92-93 0.4678729215755908 1104 94-95 0.5110305303488687 1104 96-97 0.4860773745879108 1104 98-99 0.5153600201786723 1104 100-101 0.5899438256675218 1104 102-103 0.5781113397872026 1104 104-105 0.6155555706975875 1104 106-107 0.6172161748548035 1104 108-109 0.5445452796673464 1104 110-111 0.5608649253710958 1104 112-113 0.5364560582727194 1104 114-115 0.5447231128892653 1104 116-117 0.5054343679856714 1104 118-119 0.5460100946523028 1104 120-121 0.5027882212419854 1104 122-123 0.31164461796104703 1104 124-125 0.1875033621016513 1105 1 0.853388908412775 1105 2 1.2813254858452865 1105 3 0.8043035572663051 1105 4 0.8885817876626874 1105 5 0.7620502524139745 1105 6 0.5331484009182788 1105 7 0.44922307413469653 1105 8 0.4453641128518946 1105 9 0.7269357008056048 1105 10-11 0.7023876018303561 1105 12-13 0.8852403660500414 1105 14-15 1.0485125139215334 1105 16-17 0.7006341763307127 1105 18-19 0.8080282780968027 1105 20-21 0.7872283658821964 1105 22-23 0.8665564100347183 1105 24-25 0.5780621230686158 1105 26-27 0.8291182090245748 1105 28-29 0.9627665161780676 1105 30-31 0.8893974511332843 1105 32-33 0.9735051978986 1105 34-35 0.6320859721419616 1105 36-37 0.33240917885288823 1105 38-39 0.5324577090145972 1105 40-41 0.567977180051642 1105 42-43 0.7184620889181517 1105 44-45 0.6577656938722889 1105 46-47 0.2263990020134301 1105 48-49 0.3096681906502603 1105 50-51 0.5016926110134996 1105 52-53 0.18759373299262094 1105 54-55 0.3341663153219798 1105 56-57 0.4328713519738905 1105 58-59 0.3729417575481335 1105 60-61 0.3961814797025909 1105 62-63 0.33987153867155584 1105 64-65 0.043286520078787305 1105 66-67 -0.04312083124388977 1105 68-69 -0.1450447051598367 1105 70-71 0.11561035823231336 1105 72-73 0.09693246690665092 1105 74-75 0.2202181590338057 1105 76-77 0.14941379292249835 1105 78-79 0.07212320294486929 1105 80-81 0.3236635236629368 1105 82-83 0.26902793096282807 1105 84-85 0.2542185092550149 1105 86-87 0.28643267147214857 1105 88-89 0.25237629477491197 1105 90-91 0.1564232509561947 1105 92-93 0.2748465376731133 1105 94-95 0.12008196226003065 1105 96-97 0.08795280249139026 1105 98-99 0.19822860162645028 1105 100-101 0.355344009352919 1105 102-103 0.20557723274871265 1105 104-105 0.22569325125198247 1105 106-107 0.3252196815760193 1105 108-109 0.3456572442661603 1105 110-111 0.32106447450693665 1105 112-113 0.34479011521519354 1105 114-115 0.44014767663644605 1105 116-117 0.4669531957388635 1105 118-119 0.4623654424021524 1105 120-121 0.30563559537574037 1105 122-123 0.38093751267591713 1105 124-125 0.3617763853963005 1106 1 0.23850023733542614 1106 2 -2.2669879994115973 1106 3 -0.34536319107968794 1106 4 0.2276914829987966 1106 5 0.21617195461114846 1106 6 0.31556545556174953 1106 7 0.4279093438398327 1106 8 0.3990908138238787 1106 9 0.7879908343513051 1106 10-11 1.1390162876099836 1106 12-13 1.1031257699543104 1106 14-15 1.030828694596206 1106 16-17 0.7091075080304599 1106 18-19 0.6360633185280697 1106 20-21 0.9789803874455423 1106 22-23 0.8119487158421173 1106 24-25 0.9890291246205223 1106 26-27 0.8923469928193946 1106 28-29 0.9658213230064625 1106 30-31 1.1588936530445793 1106 32-33 1.2178386945905864 1106 34-35 1.0649262073784236 1106 36-37 0.8285060505346635 1106 38-39 1.1853028450106784 1106 40-41 1.1718706702092838 1106 42-43 1.3322813325675824 1106 44-45 1.1467971404131667 1106 46-47 1.0614866439312607 1106 48-49 0.7615379121237567 1106 50-51 0.6452258953392409 1106 52-53 0.6506373497532145 1106 54-55 0.6699788612666033 1106 56-57 0.7075752644136521 1106 58-59 0.4951181032150451 1106 60-61 0.7757775754307588 1106 62-63 0.656581658740162 1106 64-65 0.6318511692660778 1106 66-67 0.5781154670490167 1106 68-69 0.5240274176858755 1106 70-71 0.6335075239596648 1106 72-73 0.5756676625286374 1106 74-75 0.5816503243527649 1106 76-77 0.43863007978029245 1106 78-79 0.5429566587971983 1106 80-81 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 263054.0 3 32249.0 4 18490.0 5 15019.0 6 14801.0 7 16408.0 8 17786.0 9 18723.0 10 19361.0 11 20310.0 12 21290.0 13 21518.0 14 20889.0 15 17681.0 16 14923.0 17 12810.0 18 11483.0 19 10851.0 20 10782.0 21 11372.0 22 14431.0 23 19840.0 24 27849.0 25 36357.0 26 41930.0 27 46360.0 28 53132.0 29 60449.0 30 69322.0 31 80244.0 32 96602.0 33 120746.0 34 156568.0 35 210885.0 36 295046.0 37 298107.0 38 67819.0 39 100.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.0399550792581 18.46622239976917 13.696134246540502 23.79768827443223 2 13.722423769186564 19.734952337920415 41.41187669712536 25.13074719576766 3 19.380009638520374 24.931902712391995 27.993976363679458 27.694111285408173 4 11.922781881503743 16.38881672260529 36.64055807552416 35.04784332036681 5 13.570813981053057 38.129757587996174 33.811426113067064 14.488002317883703 6 32.20307835894539 36.50128111450383 17.084348298236087 14.211292228314692 7 28.84927599535837 32.31554305391464 20.395362932049956 18.43981801867703 8 25.829312609725854 36.71359118580215 19.21230648349355 18.244789720978442 9 25.714659032763144 17.477768915987745 19.65288772803824 37.154684323210866 10-11 24.50570715427546 27.664470685980312 26.95390281010741 20.875919349636817 12-13 25.350373609852472 24.586410222412646 27.519403714511448 22.54381245322343 14-15 22.28073074990772 26.040988069550302 25.26413228270791 26.41414889783407 16-17 22.575872921539727 29.352730469737832 25.444881111568378 22.626515497154067 18-19 22.503018868999828 27.868506417713267 27.549840107896756 22.078634605390146 20-21 23.070531379365594 27.018927939165472 27.82227546894634 22.088265212522597 22-23 22.96232930393912 26.817420189472656 27.44553916267319 22.774711343915033 24-25 22.768588854524047 26.936803692726148 27.569860624007937 22.724746828741868 26-27 22.62408476084031 27.04164349142794 27.62057118994689 22.713700557784865 28-29 22.536163863510193 27.140074687037902 27.68774602637475 22.63601542307715 30-31 22.639993356214077 26.92602411703055 27.716419902170692 22.71756262458468 32-33 22.45178054877712 26.984610636206526 27.67402362023143 22.889585194784924 34-35 22.899469559257383 26.937068447189944 27.510565867733387 22.652896125819282 36-37 22.865992008843516 26.82208556694593 27.359029053671343 22.95289337053921 38-39 22.701530679903495 26.967479216811284 27.567472071168847 22.763518032116377 40-41 22.90066395035664 26.88272183472602 27.46709022687437 22.749523988042974 42-43 22.63425331363587 26.77344071872629 27.590568033686345 23.001737933951492 44-45 22.83766455345408 27.004638957114572 27.311112128986988 22.84658436044436 46-47 22.913389795225207 27.27267149477928 26.863603992505837 22.950334717489675 48-49 22.996622268659152 27.1218634804 26.825703891249937 23.055810359690916 50-51 22.92647703366059 27.299658134642357 26.786005434782606 22.987859396914445 52-53 23.171377249577784 27.280516500100276 26.584280326900462 22.963825923421474 54-55 23.05294175015807 27.15704785062828 26.738208288907465 23.05180211030618 56-57 22.885606484975835 27.359675260294075 26.746207907399565 23.008510347330525 58-59 23.019492904470592 27.041297122678415 26.731464081080247 23.207745891770745 60-61 23.045639740190698 27.080414795570327 26.893978986747236 22.979966477491732 62-63 22.981487308074225 27.151531856676726 26.871405020679212 22.99557581456984 64-65 22.904135400120502 27.071917620638658 26.847280495152543 23.176666484088297 66-67 22.833717471231278 27.27138099272099 26.70662051316091 23.18828102288682 68-69 22.88661017918276 27.069432635750807 26.9605309826408 23.08342620242563 70-71 22.83484145845602 26.985164991166823 26.962841058859823 23.217152491517343 72-73 23.01072503910159 27.106070498728446 26.61543067820699 23.26777378396297 74-75 22.93050926753367 27.24959786368367 26.644465938440725 23.17542693034193 76-77 23.109309328388044 27.027733992899726 26.739166391908185 23.123790286804034 78-79 23.06140293797966 27.130050590529574 26.7028869531382 23.10565951835257 80-81 22.838133196898667 27.217685964356807 26.831454617761995 23.112726220982527 82-83 22.947836086321654 27.326849217576353 26.68260086840523 23.042713827696762 84-85 23.02324592940995 27.156013834244092 26.64542366167535 23.17531657467061 86-87 22.965494374342416 27.08492079197723 26.86092887067617 23.088655963004186 88-89 23.058803832878368 27.102930100393152 26.74413737775178 23.094128688976696 90-91 22.99008611490781 27.265462755335236 26.8189369535104 22.92551417624655 92-93 22.91727013578329 27.25885154988398 26.788638639406297 23.035239674926434 94-95 22.8763611650545 27.08821188929611 26.85461101476667 23.18081593088272 96-97 23.020901058913065 27.13521975574198 26.68174735227907 23.16213183306588 98-99 22.878154556506143 27.080146967953304 26.623808317607846 23.417890157932703 100-101 22.73808313674905 27.146354223261326 26.850601495325794 23.264961144663832 102-103 22.769654866492477 27.01532431521425 26.91700779387457 23.298013024418704 104-105 22.75003023181228 26.962898994200753 27.00425961853587 23.282811155451103 106-107 22.66952234562946 27.11895264118172 26.93224977609677 23.279275237092044 108-109 22.64308617137869 27.111931249750764 26.733403476513356 23.51157910235719 110-111 22.617298575863167 27.191762862477137 26.857135722235814 23.33380283942388 112-113 22.74345038735087 27.164631949322516 26.918547129240416 23.173370534086196 114-115 22.719268196309965 27.208123539621116 26.808810941660187 23.263797322408728 116-117 22.39914083746881 27.199490250091117 27.027018147026666 23.374350765413407 118-119 22.562193750774725 27.189412312472648 26.806615837380154 23.441778099372467 120-121 22.50215427334471 27.28565938283217 26.896762403632536 23.315423940190584 122-123 22.548453093375155 27.237626061280455 26.87442500284761 23.33949584249678 124-125 22.561621370734482 27.126567297955656 26.855376535920783 23.45643479538908 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3453.0 1 2481.5 2 1178.0 3 805.0 4 681.5 5 572.5 6 588.0 7 747.0 8 952.0 9 1038.0 10 1063.5 11 1126.0 12 1221.0 13 1304.0 14 1365.5 15 1499.5 16 1665.0 17 1833.5 18 2064.5 19 2320.5 20 2771.5 21 3410.0 22 4151.5 23 5148.0 24 6496.5 25 8315.5 26 10742.5 27 13784.0 28 16800.0 29 20237.0 30 24291.0 31 28074.5 32 32152.5 33 36550.0 34 41066.5 35 45975.0 36 50889.0 37 55640.0 38 59660.0 39 62249.5 40 64972.5 41 68440.5 42 71885.5 43 75487.5 44 79359.0 45 82087.0 46 82990.5 47 84546.5 48 87755.5 49 88720.0 50 86940.5 51 84693.5 52 82811.0 53 81537.0 54 79721.5 55 75640.5 56 69461.5 57 64030.5 58 59532.5 59 53585.5 60 45761.5 61 37067.0 62 29191.0 63 22855.0 64 17323.0 65 13011.5 66 9827.5 67 7613.5 68 5935.0 69 4495.0 70 3351.0 71 2561.0 72 1976.5 73 1554.5 74 1211.5 75 841.5 76 587.5 77 414.5 78 294.5 79 216.0 80 158.0 81 119.5 82 89.5 83 64.5 84 53.0 85 43.0 86 33.0 87 22.0 88 15.5 89 13.0 90 8.5 91 4.5 92 8.5 93 9.0 94 5.0 95 4.0 96 2.5 97 1.0 98 1.5 99 1.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2242749893134674 2 0.26238336147344204 3 0.31628636319685055 4 0.3018480591637947 5 0.33457488163872123 6 0.24243225044594674 7 0.23420679239075126 8 0.21451819598203875 9 0.21368689968922647 10-11 0.15483987264540794 12-13 0.18067568637728512 14-15 0.1983297944904307 16-17 0.1710720265734798 18-19 0.1976516317252417 20-21 0.19174505280262794 22-23 0.2138837856533136 24-25 0.20434575450420395 26-27 0.2561705154955817 28-29 0.18378210936621533 30-31 0.16431227513982186 32-33 0.2039301063577978 34-35 0.1623652917171825 36-37 0.16142461433321068 38-39 0.19585778183022567 40-41 0.21524011118369155 42-43 0.28194070057276316 44-45 0.18146323023363364 46-47 0.16678428780002688 48-49 0.16967194860663803 50-51 0.17444096418119284 52-53 0.19533275259266 54-55 0.18220702165351832 56-57 0.18065381015905324 58-59 0.15538677810120552 60-61 0.16785622249339008 62-63 0.156852484722743 64-65 0.15147093503769493 66-67 0.14016093021180118 68-69 0.14230479959852765 70-71 0.14363924891067373 72-73 0.12038482893016104 74-75 0.1215880209329157 76-77 0.1434861153830504 78-79 0.1505302576537231 80-81 0.16033080342161554 82-83 0.16201527222547205 84-85 0.1498083424520703 86-87 0.1763879476038322 88-89 0.1709626454823203 90-91 0.15908385898239708 92-93 0.1593463736011799 94-95 0.18076319125021276 96-97 0.16890628096852142 98-99 0.163152835573531 100-101 0.14328922941896327 102-103 0.18288518441870733 104-105 0.14101410272284537 106-107 0.1466144145902125 108-109 0.15698374203213442 110-111 0.13329179768698368 112-113 0.12414753846604833 114-115 0.12202554529755377 116-117 0.12683831330857237 118-119 0.11025613988879006 120-121 0.10214006292475412 122-123 0.12981347898811116 124-125 0.12585388348813675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2285587.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.90525619946156 #Duplication Level Percentage of deduplicated Percentage of total 1 84.97567661465914 59.40246444470342 2 8.919659788933657 12.470622055148851 3 2.656864831922968 5.571864502887434 4 1.1411812190562844 3.1909826195257374 5 0.5967751866112477 2.0858861156770376 6 0.3740936848873839 1.5690668930791927 7 0.24949719460865766 1.2208815717115595 8 0.17882066899520374 1.0000403743895065 9 0.13997251896710805 0.8806333319351713 >10 0.7166837557967106 8.913424593819116 >50 0.03708220802496548 1.7475811896850177 >100 0.013178935223202157 1.4933662176970184 >500 2.566961567289447E-4 0.12300893467354658 >1k 2.56696156728056E-4 0.3301771550674508 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 4.375243646380558E-5 6 0.0 0.0 0.0 0.0 4.375243646380558E-5 7 0.0 0.0 0.0 0.0 4.375243646380558E-5 8 0.0 0.0 0.0 0.0 4.375243646380558E-5 9 0.0 0.0 0.0 0.0 4.375243646380558E-5 10-11 0.0 0.0 0.0 0.0 4.375243646380558E-5 12-13 8.750487292761116E-5 0.0 0.0 0.0 4.375243646380558E-5 14-15 8.750487292761116E-5 0.0 0.0 0.0 6.562865469570837E-5 16-17 8.750487292761116E-5 0.0 0.0 0.0 1.0938109115951394E-4 18-19 8.750487292761116E-5 4.375243646380558E-5 0.0 8.750487292761116E-5 1.3125730939141672E-4 20-21 8.750487292761116E-5 8.750487292761116E-5 0.0 2.1876218231902788E-4 1.3125730939141672E-4 22-23 8.750487292761116E-5 8.750487292761116E-5 0.0 2.4063840055093066E-4 1.3125730939141672E-4 24-25 1.3125730939141672E-4 8.750487292761116E-5 0.0 2.6251461878283345E-4 1.3125730939141672E-4 26-27 1.531335276233195E-4 8.750487292761116E-5 0.0 3.937719281742502E-4 1.3125730939141672E-4 28-29 1.7500974585522231E-4 8.750487292761116E-5 0.0 7.21915201652792E-4 1.3125730939141672E-4 30-31 1.7500974585522231E-4 8.750487292761116E-5 0.0 0.0018813547679436397 1.3125730939141672E-4 32-33 1.968859640871251E-4 8.750487292761116E-5 0.0 0.004615882046931488 2.1876218231902788E-4 34-35 2.1876218231902788E-4 8.750487292761116E-5 0.0 0.007831686127021198 2.1876218231902788E-4 36-37 2.1876218231902788E-4 8.750487292761116E-5 0.0 0.013038226066214062 2.1876218231902788E-4 38-39 2.1876218231902788E-4 8.750487292761116E-5 0.0 0.023735696781614524 2.1876218231902788E-4 40-41 2.6251461878283345E-4 8.750487292761116E-5 0.0 0.036642665538437175 2.1876218231902788E-4 42-43 2.6251461878283345E-4 8.750487292761116E-5 0.0 0.048543328256592286 2.1876218231902788E-4 44-45 2.6251461878283345E-4 8.750487292761116E-5 0.0 0.06282849876202481 2.1876218231902788E-4 46-47 2.6251461878283345E-4 8.750487292761116E-5 0.0 0.07914815756302429 2.1876218231902788E-4 48-49 2.6251461878283345E-4 8.750487292761116E-5 0.0 0.09767731440544596 2.6251461878283345E-4 50-51 2.6251461878283345E-4 8.750487292761116E-5 0.0 0.119269141800334 2.6251461878283345E-4 52-53 2.8439083701473625E-4 8.750487292761116E-5 0.0 0.13939526257368456 3.06267055246639E-4 54-55 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.16343722641054576 3.5001949171044463E-4 56-57 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.19172317658439603 3.5001949171044463E-4 58-59 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.22471251367810546 3.7189570994234744E-4 60-61 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.26277713340161635 3.937719281742502E-4 62-63 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.3025918505836794 3.937719281742502E-4 64-65 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.3426472061662934 3.937719281742502E-4 66-67 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.3879091016881002 3.937719281742502E-4 68-69 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.4422277515579149 3.937719281742502E-4 70-71 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.5019935797674733 4.5940058286995857E-4 72-73 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.5662221564963399 5.031530193337641E-4 74-75 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.6317414301008888 5.250292375656669E-4 76-77 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.7090301091142013 5.250292375656669E-4 78-79 3.0626705524663906E-4 8.750487292761116E-5 0.0 0.7979569362268861 5.250292375656669E-4 80-81 3.5001949171044463E-4 8.750487292761116E-5 0.0 0.8988938071488857 5.469054557975697E-4 82-83 3.5001949171044463E-4 8.750487292761116E-5 0.0 1.0123438748995335 5.687816740294725E-4 84-85 3.7189570994234744E-4 8.750487292761116E-5 0.0 1.124962646357369 5.687816740294725E-4 86-87 3.937719281742502E-4 8.750487292761116E-5 0.0 1.2443849216853264 5.687816740294725E-4 88-89 3.937719281742502E-4 8.750487292761116E-5 0.0 1.3827957544385754 5.687816740294725E-4 90-91 4.3752436463805576E-4 8.750487292761116E-5 0.0 1.5434328249154374 5.687816740294725E-4 92-93 4.3752436463805576E-4 8.750487292761116E-5 0.0 1.7131485259585393 5.687816740294725E-4 94-95 4.3752436463805576E-4 8.750487292761116E-5 0.0 1.886233164609354 6.125341104932781E-4 96-97 4.3752436463805576E-4 8.750487292761116E-5 0.0 2.068046414334698 6.125341104932781E-4 98-99 4.5940058286995857E-4 1.3125730939141672E-4 0.0 2.2664637136980565 6.125341104932781E-4 100-101 5.031530193337641E-4 1.3125730939141672E-4 0.0 2.5046082253705504 6.125341104932781E-4 102-103 5.250292375656669E-4 1.3125730939141672E-4 0.0 2.764541450402019 6.344103287251809E-4 104-105 6.125341104932781E-4 1.3125730939141672E-4 0.0 3.0231183499031102 6.562865469570836E-4 106-107 6.125341104932781E-4 1.3125730939141672E-4 0.0 3.2877549618544384 6.781627651889864E-4 108-109 6.125341104932781E-4 1.7500974585522231E-4 0.0 3.569805043518361 7.000389834208893E-4 110-111 6.125341104932781E-4 1.7500974585522231E-4 0.0 3.8890446961765184 7.000389834208893E-4 112-113 6.125341104932781E-4 1.7500974585522231E-4 0.0 4.248558466599609 7.000389834208893E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5415 0.0 46.212135 1 GGTATCA 3320 0.0 46.121162 1 ATCAACG 6485 0.0 38.125427 3 TCAACGC 6490 0.0 37.82066 4 CAACGCA 6600 0.0 36.919514 5 AACGCAG 6690 0.0 36.030243 6 TATCAAC 7040 0.0 34.27354 2 GTACATG 8210 0.0 33.310005 1 TACATGG 8355 0.0 31.23232 2 ACGCAGA 7840 0.0 30.133257 7 ACATGGG 8645 0.0 29.357643 3 CGCAGAG 7745 0.0 28.885569 8 GTACCTG 2365 0.0 24.940737 1 TACCTGG 2310 0.0 24.50125 2 CATGGGA 4725 0.0 24.082867 4 GCAGAGT 8730 0.0 23.514126 9 GAGTACA 6705 0.0 23.103605 1 GTATAGG 780 0.0 22.915632 1 AGTACAT 6270 0.0 21.474224 2 CAGAGTA 8620 0.0 20.981339 10-11 >>END_MODULE