FastQCFastQC Report
Thu 26 May 2016
SRR1364625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2285587
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55480.24273851750119335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48470.21206805954006566No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32590.14258919043554238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45550.049.173591
GTATCAA74800.045.1558381
ATCAACG92050.035.6799933
TCAACGC92450.035.593094
TATCAAC94700.035.0194552
CAACGCA93850.034.653195
AACGCAG96000.034.1178136
GTACATG85450.031.5804711
TACATGG87400.029.6334322
ACGCAGA116950.027.7005527
CGCAGAG117000.027.4342238
ACATGGG90150.026.8619883
GTGGTAT29600.025.156531
TGGTATC28500.023.6068342
GCAGAGT132900.023.2568659
GAGTACT79250.021.38015212-13
GTACTTT80650.020.97209414-15
AGTACTT80550.020.62844312-13
AGAGTAC124650.020.55480210-11
ATACAGT23900.020.4317576
Produced by FastQC (version 0.11.5)