##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364625_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2285587 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.336629933579427 31.0 30.0 33.0 26.0 34.0 2 29.456778061828317 31.0 30.0 34.0 25.0 34.0 3 29.56570150250242 31.0 30.0 34.0 25.0 34.0 4 33.24185778095518 35.0 33.0 37.0 28.0 37.0 5 32.568453530755995 35.0 33.0 37.0 25.0 37.0 6 32.58034019269448 35.0 33.0 37.0 25.0 37.0 7 32.36861602730502 35.0 33.0 37.0 23.0 37.0 8 32.3122992036619 35.0 33.0 37.0 22.0 37.0 9 33.5885035222899 38.0 33.0 39.0 22.0 39.0 10-11 33.38390334736766 37.0 33.0 39.0 18.5 39.0 12-13 33.2365829872151 37.0 33.0 39.0 17.5 39.0 14-15 34.2198314918662 38.0 33.0 41.0 17.5 41.0 16-17 34.12767836008868 38.0 33.0 41.0 17.5 41.0 18-19 34.15109794551684 38.5 33.0 41.0 17.5 41.0 20-21 34.16609737454755 39.0 33.0 41.0 18.0 41.0 22-23 34.15399566938383 39.0 33.0 41.0 18.0 41.0 24-25 34.14618826585905 39.0 33.0 41.0 18.0 41.0 26-27 34.06303654159741 39.0 33.0 41.0 18.0 41.0 28-29 33.83204008423219 38.0 33.0 41.0 17.0 41.0 30-31 33.783683141354935 38.0 33.0 41.0 16.5 41.0 32-33 33.62631525293065 38.0 33.0 40.5 16.0 41.0 34-35 33.49441500148539 38.0 32.0 40.0 15.5 41.0 36-37 33.335917643913795 38.0 32.0 40.0 11.0 41.0 38-39 33.20656903456312 38.0 32.0 40.0 9.0 41.0 40-41 33.04346957696207 38.0 32.0 40.0 9.0 41.0 42-43 32.87820371746952 38.0 32.0 40.0 8.0 41.0 44-45 32.68223021044484 38.0 31.0 40.0 4.5 41.0 46-47 32.454562875970154 38.0 31.0 40.0 2.0 41.0 48-49 32.22164568664417 38.0 31.0 40.0 2.0 41.0 50-51 32.02750737556698 38.0 30.0 40.0 2.0 41.0 52-53 31.85417750450978 37.0 30.0 40.0 2.0 41.0 54-55 31.630645431567473 37.0 30.0 40.0 2.0 41.0 56-57 31.401186216057408 37.0 30.0 40.0 2.0 41.0 58-59 31.206138729350492 36.5 29.5 40.0 2.0 41.0 60-61 30.982715381212792 36.0 29.0 40.0 2.0 41.0 62-63 30.710970748433553 36.0 29.0 39.5 2.0 41.0 64-65 30.4297499942028 35.0 29.0 39.0 2.0 41.0 66-67 30.146996592122726 35.0 28.5 39.0 2.0 40.5 68-69 29.83397197306425 35.0 28.0 38.5 2.0 40.0 70-71 29.57240481329304 35.0 28.0 38.0 2.0 40.0 72-73 29.41691390439305 35.0 28.0 37.0 2.0 39.5 74-75 29.13377723096955 34.5 28.0 37.0 2.0 39.0 76-77 28.844739885202358 34.0 28.0 36.0 2.0 39.0 78-79 28.552889257770545 34.0 28.0 36.0 2.0 38.5 80-81 28.287752555470433 34.0 27.5 35.5 2.0 37.0 82-83 28.043908851424163 34.0 27.0 35.0 2.0 37.0 84-85 28.038612181465858 34.0 28.0 35.0 2.0 36.5 86-87 27.902566386665658 34.0 27.5 35.0 2.0 36.0 88-89 27.76673651013941 34.0 27.0 35.0 2.0 36.0 90-91 27.621248720788138 34.0 27.0 35.0 2.0 36.0 92-93 27.49237679423273 34.0 27.0 35.0 2.0 35.0 94-95 27.411180366356653 34.0 27.0 35.0 2.0 35.0 96-97 27.335034719745956 34.0 27.0 35.0 2.0 35.0 98-99 27.244428674121792 34.0 26.0 35.0 2.0 35.0 100-101 27.150264680364387 34.0 26.0 35.0 2.0 35.0 102-103 25.976836585087334 32.0 24.5 34.0 2.0 35.0 104-105 26.902850121216126 33.5 25.5 35.0 2.0 35.0 106-107 27.07690387633461 34.0 26.0 35.0 2.0 35.0 108-109 27.09128748982209 34.0 26.0 35.0 2.0 35.0 110-111 27.034772905166157 34.0 26.0 35.0 2.0 35.0 112-113 26.98660847300934 34.0 26.0 35.0 2.0 35.0 114-115 26.904431115507744 34.0 25.0 35.0 2.0 35.0 116-117 26.79385864550332 34.0 25.0 35.0 2.0 35.0 118-119 26.695614518283485 34.0 25.0 35.0 2.0 35.0 120-121 26.565022464688504 34.0 25.0 35.0 2.0 35.0 122-123 26.38441590716083 34.0 25.0 35.0 2.0 35.0 124-125 25.579600120231696 33.0 22.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6136904489419521 1101 2 0.5412681850911163 1101 3 0.4438574327670075 1101 4 0.6061558230259152 1101 5 1.0055389200352849 1101 6 0.9065353724291398 1101 7 0.9557390907472012 1101 8 0.9653221039367992 1101 9 1.0965665668693774 1101 10-11 1.132555684467924 1101 12-13 1.1498935973117526 1101 14-15 1.2375964766729055 1101 16-17 1.325849436868971 1101 18-19 1.3707645670810322 1101 20-21 1.479432610380627 1101 22-23 1.3616873949903479 1101 24-25 1.2470296808858379 1101 26-27 1.398232398457381 1101 28-29 1.4385684523549145 1101 30-31 1.3582462441906173 1101 32-33 1.4634179270311236 1101 34-35 1.3256522455626367 1101 36-37 1.4113685554812179 1101 38-39 1.4069832174850276 1101 40-41 1.385985646936021 1101 42-43 1.4100863986131387 1101 44-45 1.3781129409040673 1101 46-47 1.3960359346694275 1101 48-49 1.5256628980649296 1101 50-51 1.4155311293565092 1101 52-53 1.5115843755456986 1101 54-55 1.4761757924885472 1101 56-57 1.3835020106631042 1101 58-59 1.421536134204171 1101 60-61 1.3961945020272353 1101 62-63 1.3826086298895497 1101 64-65 1.362812149844622 1101 66-67 1.3956453892215066 1101 68-69 1.39714349380872 1101 70-71 1.3455157303445446 1101 72-73 1.3161262658643764 1101 74-75 1.2497584417742864 1101 76-77 1.2360217082434808 1101 78-79 1.3033904738132023 1101 80-81 1.220319654421477 1101 82-83 1.2242741197119145 1101 84-85 1.2816065373153336 1101 86-87 1.3095341713436994 1101 88-89 1.3535382694239395 1101 90-91 1.4025572680820808 1101 92-93 1.421552503739406 1101 94-95 1.2988686975443997 1101 96-97 1.3524374409231434 1101 98-99 1.391372774237393 1101 100-101 1.4166651710531006 1101 102-103 1.346570259344027 1101 104-105 1.3217000347870282 1101 106-107 1.2704304182962325 1101 108-109 1.2305538729575325 1101 110-111 1.2571309501130017 1101 112-113 1.2594721835105105 1101 114-115 1.2871747966526286 1101 116-117 1.3312437054663384 1101 118-119 1.4418478412858846 1101 120-121 1.4858093666561487 1101 122-123 1.539062556408421 1101 124-125 1.2985467395309591 1102 1 0.6681294786179066 1102 2 -0.2253507396173582 1102 3 0.3867824079698998 1102 4 0.6659001835939264 1102 5 0.9734832744908459 1102 6 0.85115412209921 1102 7 0.8623005306187324 1102 8 0.9259002585874043 1102 9 1.2230102864633992 1102 10-11 1.1862917821115744 1102 12-13 1.2389054915885538 1102 14-15 1.2905223270678547 1102 16-17 1.3910753822847681 1102 18-19 1.316976201022932 1102 20-21 1.3404088732271973 1102 22-23 1.3945159532017328 1102 24-25 1.3482558874700672 1102 26-27 1.292454672234122 1102 28-29 1.4300466170077115 1102 30-31 1.3033663191515075 1102 32-33 1.4036087953049972 1102 34-35 1.2199205363820127 1102 36-37 1.1232917969703209 1102 38-39 1.1674422835022895 1102 40-41 1.2469749334738935 1102 42-43 1.1934874782945144 1102 44-45 1.082996095818622 1102 46-47 1.104185257715315 1102 48-49 1.235086806367029 1102 50-51 1.1244066534298511 1102 52-53 1.109685069864728 1102 54-55 1.2025680077246719 1102 56-57 1.0974437506560513 1102 58-59 1.1383059253527854 1102 60-61 0.9599472074820454 1102 62-63 1.1103349920634678 1102 64-65 1.20819749135012 1102 66-67 1.1657970336101364 1102 68-69 0.9440683745464362 1102 70-71 0.6979843970797432 1102 72-73 0.8631601983106627 1102 74-75 1.0111831710898862 1102 76-77 1.0035696562654053 1102 78-79 0.9332498735148036 1102 80-81 0.791279323348828 1102 82-83 0.7271417628891683 1102 84-85 0.9209155801330589 1102 86-87 0.8271335195339979 1102 88-89 0.9235213351522482 1102 90-91 0.9506841487031075 1102 92-93 1.0197454057929107 1102 94-95 0.8219867915150161 1102 96-97 0.8080879517296786 1102 98-99 0.7919094545088932 1102 100-101 0.7795753652346029 1102 102-103 0.6963032563072495 1102 104-105 0.7132224105305944 1102 106-107 0.9513541487815331 1102 108-109 1.0160034924573687 1102 110-111 0.9185318200261214 1102 112-113 0.9283573346964644 1102 114-115 0.9881527543155606 1102 116-117 1.0105929584968614 1102 118-119 0.8747270356090837 1102 120-121 0.8286608187803637 1102 122-123 0.8282909565295604 1102 124-125 0.641690095989734 1103 1 0.2692426918452391 1103 2 -0.9908922867826959 1103 3 -0.004162203996678215 1103 4 0.3358509000405121 1103 5 0.48063809137399005 1103 6 0.6630239112037728 1103 7 0.512094322841854 1103 8 0.5976432318389868 1103 9 0.7007074259401378 1103 10-11 0.8877040966140513 1103 12-13 0.9968464383408389 1103 14-15 1.040216717107569 1103 16-17 1.1321977374954741 1103 18-19 1.117052594741189 1103 20-21 1.1673788557327 1103 22-23 1.0793735322936584 1103 24-25 0.9233271233623626 1103 26-27 0.9956832783088458 1103 28-29 1.1694361883046582 1103 30-31 1.0537576735825738 1103 32-33 1.0673726205592544 1103 34-35 1.074192786436221 1103 36-37 1.1787613205345693 1103 38-39 1.1376456193876336 1103 40-41 1.1420054558739494 1103 42-43 1.1746850974139775 1103 44-45 1.244092105766562 1103 46-47 1.2685999514843118 1103 48-49 1.2348524673862897 1103 50-51 1.208122861775145 1103 52-53 1.2554505824649205 1103 54-55 1.2421351556012965 1103 56-57 1.188465344052343 1103 58-59 1.2194892288736447 1103 60-61 1.2333991316318347 1103 62-63 1.1545554550888717 1103 64-65 1.2023975248233576 1103 66-67 1.1397216795271383 1103 68-69 1.0797848983261176 1103 70-71 1.135341635825334 1103 72-73 1.0857844229701215 1103 74-75 1.013993923086879 1103 76-77 0.9387097315907162 1103 78-79 1.0163901684863106 1103 80-81 1.1053726792970622 1103 82-83 1.0683533537521654 1103 84-85 1.1200442408737246 1103 86-87 1.0244700989059226 1103 88-89 1.074353622300702 1103 90-91 1.1140417951625565 1103 92-93 1.1037467857745114 1103 94-95 1.0063924468080359 1103 96-97 0.9379687199861024 1103 98-99 1.0665049599021579 1103 100-101 1.080167936281022 1103 102-103 0.9957794387300787 1103 104-105 1.045590435097285 1103 106-107 1.0469246288944305 1103 108-109 1.0879643312251304 1103 110-111 1.0892043250944745 1103 112-113 1.1121751908092605 1103 114-115 1.00143507721792 1103 116-117 1.0845856999971524 1103 118-119 1.1662439820497958 1103 120-121 1.1635926249527735 1103 122-123 1.142903779235784 1103 124-125 1.1718458342641007 1104 1 0.07289947034628597 1104 2 -0.899687719624584 1104 3 -0.08328631762847039 1104 4 0.1663186467461557 1104 5 0.45963737494152923 1104 6 0.44415284997122484 1104 7 0.47866885630580924 1104 8 0.44364170136466186 1104 9 0.40266628185472797 1104 10-11 0.5707800853690301 1104 12-13 0.4859235929096357 1104 14-15 0.40459956699649524 1104 16-17 0.6165730570245245 1104 18-19 0.5129183463998146 1104 20-21 0.6054063310635485 1104 22-23 0.5591859984933905 1104 24-25 0.5467476939615423 1104 26-27 0.5147058575310055 1104 28-29 0.7648608158809367 1104 30-31 0.5986535348232351 1104 32-33 0.6606476070649308 1104 34-35 0.6447681286147784 1104 36-37 0.6064477832949464 1104 38-39 0.6942693459328098 1104 40-41 0.8183612600509065 1104 42-43 0.6745984003222745 1104 44-45 0.5589656749423355 1104 46-47 0.4886823661349382 1104 48-49 0.3900934913158878 1104 50-51 0.4967531611456124 1104 52-53 0.32369880801001827 1104 54-55 0.4984759437388604 1104 56-57 0.6234997075487563 1104 58-59 0.4066136882627198 1104 60-61 0.3794011584018975 1104 62-63 0.41403950686241586 1104 64-65 0.4245806899150715 1104 66-67 0.3557100839414886 1104 68-69 0.4760346003854181 1104 70-71 0.41406604063562114 1104 72-73 0.3976408646155889 1104 74-75 0.36561640459650846 1104 76-77 0.413004989062177 1104 78-79 0.3673778451262635 1104 80-81 0.29052016144682824 1104 82-83 0.37979293619010335 1104 84-85 0.38648983711686213 1104 86-87 0.43446077445756615 1104 88-89 0.40076129866226395 1104 90-91 0.35340576330133544 1104 92-93 0.3243453445578055 1104 94-95 0.27181297238316304 1104 96-97 0.34324888219644833 1104 98-99 0.39702731116852874 1104 100-101 0.434587193793039 1104 102-103 0.48958141294916757 1104 104-105 0.4761361834083253 1104 106-107 0.5001915884007886 1104 108-109 0.4331316600490709 1104 110-111 0.34954843092094734 1104 112-113 0.38104353685043435 1104 114-115 0.4678526709019053 1104 116-117 0.5055413236310713 1104 118-119 0.37190877345866014 1104 120-121 0.3952063113045199 1104 122-123 0.5131027666756189 1104 124-125 0.4437939892314162 1105 1 0.5618853599680627 1105 2 0.32263289488656 1105 3 0.5685655651468515 1105 4 0.6192141965499403 1105 5 0.7123308708086071 1105 6 0.6846062189283941 1105 7 0.7524778234929173 1105 8 0.6752033612127022 1105 9 0.7504790897365012 1105 10-11 0.9285628356403848 1105 12-13 0.8524189590280358 1105 14-15 0.8888411132935659 1105 16-17 1.0126902817051615 1105 18-19 0.9736050793045479 1105 20-21 1.0108560263127728 1105 22-23 0.8348144530312567 1105 24-25 0.963098199096386 1105 26-27 0.9196483306043959 1105 28-29 1.1239635962098973 1105 30-31 0.9435499195606454 1105 32-33 1.0082775644833148 1105 34-35 1.0813271166752259 1105 36-37 1.0791458712969515 1105 38-39 1.0704738211770248 1105 40-41 0.9453179269958838 1105 42-43 1.0479385522801934 1105 44-45 1.086108949551999 1105 46-47 1.0871724721715026 1105 48-49 0.9986993191525784 1105 50-51 0.9652602163347268 1105 52-53 0.9133280974815143 1105 54-55 0.900810251161424 1105 56-57 0.9517792433256638 1105 58-59 0.8272939921785571 1105 60-61 0.8935389224496575 1105 62-63 0.8547185583778223 1105 64-65 0.8955304269109767 1105 66-67 0.9491178655228545 1105 68-69 0.8960458719893261 1105 70-71 0.8290041720541694 1105 72-73 0.7349150568173108 1105 74-75 0.7019049576565415 1105 76-77 0.8586797824161039 1105 78-79 0.894191571435023 1105 80-81 0.7395957229396899 1105 82-83 0.7800319776863063 1105 84-85 0.6702742572554605 1105 86-87 0.7323316102261224 1105 88-89 0.8951664084560349 1105 90-91 0.8740179366335532 1105 92-93 0.7341881265566386 1105 94-95 0.7252038048119829 1105 96-97 0.7366694382622399 1105 98-99 0.7553852472126117 1105 100-101 0.8422964699055875 1105 102-103 0.42384399957353125 1105 104-105 0.3385214217818735 1105 106-107 0.6709501255970807 1105 108-109 0.6710404852974605 1105 110-111 0.6905152840561257 1105 112-113 0.5458171028519345 1105 114-115 0.6420543908651659 1105 116-117 0.336640179772008 1105 118-119 0.293954277341097 1105 120-121 0.26692761046123437 1105 122-123 0.2928054468125474 1105 124-125 0.056111848461608105 1106 1 0.43718912538503574 1106 2 0.40598615797567206 1106 3 0.5532041341250427 1106 4 0.44703662569312996 1106 5 0.6999082619709043 1106 6 0.5681159796079669 1106 7 0.6756428974127076 1106 8 0.6291667688742208 1106 9 0.7595303026745057 1106 10-11 0.9095825203581853 1106 12-13 0.6769403181720364 1106 14-15 0.8202959037860893 1106 16-17 0.9332968323871853 1106 18-19 0.7339738614657847 1106 20-21 0.8973226603077151 1106 22-23 0.8283807358050836 1106 24-25 0.8706845749846508 1106 26-27 0.7769901585877363 1106 28-29 0.9023948630562586 1106 30-31 0.7903141113437044 1106 32-33 0.6984698376189868 1106 34-35 0.6930070153928582 1106 36-37 0.7043157642970286 1106 38-39 0.7403007426831962 1106 40-41 0.8223872727444785 1106 42-43 0.7926126517656158 1106 44-45 0.6528732230143888 1106 46-47 0.41424557516913296 1106 48-49 0.5341360585138446 1106 50-51 0.6186392084118353 1106 52-53 0.6782215059598222 1106 54-55 0.6112470722020191 1106 56-57 0.47543471992453945 1106 58-59 0.5247176628475145 1106 60-61 0.7263820164625621 1106 62-63 0.6933882471795876 1106 64-65 0.5591067562420271 1106 66-67 0.480772280290477 1106 68-69 0.6027333668843688 1106 70-71 0.6355608983467462 1106 72-73 0.7565929952310988 1106 74-75 0.6916662906631572 1106 76-77 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-2.0461845345119585 2216 108-109 -1.9903617031199374 2216 110-111 -1.8837448859846688 2216 112-113 -1.895057920976651 2216 114-115 -1.9166260010674243 2216 116-117 -1.974235835325782 2216 118-119 -2.1080760326211596 2216 120-121 -2.0571950019143763 2216 122-123 -2.109685040815652 2216 124-125 -2.0721159528270405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 102375.0 3 30385.0 4 13649.0 5 10460.0 6 10925.0 7 12617.0 8 14342.0 9 15314.0 10 16700.0 11 17730.0 12 19209.0 13 21115.0 14 22304.0 15 18743.0 16 13476.0 17 11671.0 18 10555.0 19 9774.0 20 9680.0 21 10220.0 22 12016.0 23 16318.0 24 22319.0 25 29342.0 26 35533.0 27 39762.0 28 46256.0 29 54145.0 30 63241.0 31 74449.0 32 90133.0 33 111795.0 34 143457.0 35 196437.0 36 313590.0 37 472123.0 38 172751.0 39 676.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.693365567567994 19.79769836356679 13.366539498466365 25.14239657039885 2 15.502198511440055 20.198369572356466 38.46383555957612 25.835596356627367 3 19.273473291543922 24.213604645108674 28.937248943050513 27.575673120296884 4 13.253431685491293 16.72596354325055 35.577513364222256 34.4430914070359 5 13.559425143431852 38.16156013240065 33.686451341272175 14.592563382895316 6 32.827930375324485 36.87690023797138 16.386379059218953 13.90879032748519 7 28.875776769818867 32.19426781828913 20.118420344532936 18.811535067359063 8 26.120773350565962 37.03998141396499 18.326933081085954 18.5123121543831 9 25.811054111109893 16.579897837524474 19.30516390842567 38.30388414293996 10-11 25.243821609874917 27.11263906083917 26.39041139068719 21.253127938598716 12-13 25.91222447081989 23.957731225728818 27.462145162172508 22.667899141278784 14-15 22.731864090478 25.454233198091814 25.271290694151066 26.542612017279122 16-17 22.50898981981838 28.74314997508087 25.922024243238727 22.82583596186202 18-19 22.4383401437751 27.137249634502524 28.30086934036757 22.123540881354806 20-21 23.039012882212674 26.352297591561598 28.51893607157383 22.089753454651902 22-23 22.74572369306289 26.110868375665202 28.462839925952682 22.680568005319223 24-25 22.61274674733449 26.27554759455667 28.549405470017113 22.562300188091726 26-27 22.46600107543489 26.51073881676786 28.423945358457146 22.599314749340106 28-29 22.38100647357352 26.45751707802542 28.573986245653476 22.58749020274759 30-31 22.47453280054533 26.34216067907282 28.60869002142557 22.574616498956285 32-33 22.382062479681785 26.341067708709343 28.49798141422203 22.778888397386844 34-35 22.638079408047034 26.326212915981763 28.50442796533232 22.531279710638884 36-37 22.695760946560195 26.224994870013358 28.328114383169968 22.75112980025648 38-39 22.582404432646843 26.42246827620213 28.37131117739119 22.623816113759833 40-41 22.702351737212368 26.32266896862819 28.28465947697462 22.69031981718482 42-43 22.527708124318263 26.365782681498008 28.166947492497336 22.939561701686397 44-45 22.677631610610316 26.50069763259942 27.98766793825831 22.834002818531957 46-47 22.77913337647331 26.734885584990785 27.55071681929731 22.935264219238594 48-49 22.86204751921886 26.741783980043582 27.41684224204748 22.979326258690076 50-51 22.84785416001717 26.934290233939095 27.285121217935014 22.932734388108717 52-53 23.061118322983674 26.929584267996393 27.133864751356658 22.875432657663275 54-55 22.925117267467833 26.79445149101741 27.273037517276745 23.007393724238018 56-57 22.737044613638187 27.043215722461007 27.30818528880742 22.911554375093385 58-59 22.900144645618237 26.769589943235566 27.26449584482929 23.06576956631691 60-61 22.95027491843452 26.7374639425233 27.41610798451339 22.896153154528793 62-63 22.83196833023639 26.866883649583233 27.387559519720757 22.91358850045962 64-65 22.774580307926957 26.692938690031763 27.44966309345856 23.082817908582722 66-67 22.642870299839824 26.968192416215174 27.337944256770797 23.0509930271742 68-69 22.662461164073665 26.70796039687134 27.573015479242223 23.056562959812773 70-71 22.62751319464103 26.5784457121956 27.492390357488034 23.30165073567534 72-73 22.794778301392498 26.7979040923841 27.194140979561816 23.21317662666159 74-75 22.714316373805744 26.973942564561654 27.187465338099965 23.12427572353264 76-77 22.9118606981761 26.77151143292733 27.272721306440257 23.043906562456314 78-79 22.849432961724915 26.876739179894642 27.173121685713785 23.100706172666655 80-81 22.744025228090862 26.949822744878414 27.20041915558526 23.105732871445465 82-83 22.847370418700702 27.023920597921975 27.097381083378085 23.031327899999233 84-85 22.830161891109015 26.86898443042563 27.078909309433342 23.22194436903201 86-87 22.818337975493247 26.84936037949329 27.25107565976862 23.081225985244842 88-89 22.789703476612356 26.83096289924645 27.185204501075656 23.19412912306554 90-91 22.783162487361015 26.97672414132562 27.247070446235476 22.993042925077887 92-93 22.74404144624284 26.950209375287155 27.218918599614067 23.086830578855942 94-95 22.67687019316808 26.84877477609139 27.216781692984966 23.25757333775557 96-97 22.84009053109901 26.822758791941464 27.099952852943847 23.23719782401568 98-99 22.622029854860358 26.850910554645857 26.995776741560867 23.531282848932918 100-101 22.534229299059298 26.85296085870601 27.210769614782425 23.40204022745226 102-103 22.62436094041569 26.743109532132912 27.22025214551198 23.41227738193942 104-105 22.608620131052735 26.642671177871062 27.26100749113404 23.48770119994216 106-107 22.49596963062805 26.80527622332567 27.26861829377493 23.43013585227135 108-109 22.52644281993154 26.75760030069095 27.041401450860086 23.674555428517422 110-111 22.4805407888692 26.810220734615303 27.08691562206034 23.622322854455163 112-113 22.602899310405782 26.807868562961435 27.13062429705516 23.45860782957762 114-115 22.54107720861368 26.86042980887952 27.025661824994508 23.57283115751229 116-117 22.25186779116617 26.78131742856824 27.271183960065116 23.695630820200474 118-119 22.348303783810508 26.794613114295757 27.051382544885428 23.805700557008304 120-121 22.2862397734314 26.880100156483564 27.063561383916852 23.770098686168183 122-123 22.30677281772249 26.878521037405473 27.020675129115645 23.794031015756396 124-125 22.286646734057175 26.795723276108934 26.97369039933547 23.943939590498413 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 144.0 1 130.0 2 190.0 3 328.5 4 293.0 5 244.0 6 590.5 7 1296.5 8 1993.5 9 2342.5 10 2015.0 11 1513.5 12 1430.5 13 1535.0 14 1707.0 15 1899.0 16 2083.0 17 2279.5 18 2508.0 19 2800.0 20 3108.0 21 3572.5 22 4388.0 23 5342.5 24 6517.5 25 8249.0 26 10635.0 27 13603.5 28 16687.0 29 19965.0 30 23839.5 31 27545.5 32 31651.5 33 35897.5 34 40148.0 35 44729.5 36 49692.0 37 55041.0 38 59101.5 39 61683.5 40 64658.0 41 68157.0 42 71411.5 43 75113.0 44 79109.5 45 82000.5 46 82566.0 47 84703.0 48 89058.0 49 90126.0 50 88261.0 51 85804.5 52 83675.5 53 82775.0 54 82069.0 55 77634.0 56 70671.0 57 65406.5 58 61053.5 59 55127.0 60 46749.0 61 37367.0 62 28929.5 63 22082.0 64 16327.0 65 11991.0 66 8781.0 67 6484.0 68 4985.5 69 3778.0 70 2743.0 71 2029.5 72 1514.0 73 1220.5 74 931.0 75 621.5 76 421.5 77 229.5 78 134.0 79 83.0 80 54.5 81 35.0 82 20.0 83 15.5 84 11.5 85 5.5 86 3.0 87 3.5 88 2.0 89 0.5 90 1.5 91 1.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06444733891118562 2 0.07739806010447207 3 0.0 4 0.008487972673978283 5 0.03080171527051913 6 7.875438563485004E-4 7 0.0 8 0.0 9 5.250292375656669E-4 10-11 0.05840950267918044 12-13 0.009275516530326782 14-15 0.007416037980615046 16-17 0.052393542665407175 18-19 1.5313352762331953E-4 20-21 0.009909926859051964 22-23 0.012753835229199324 24-25 0.0 26-27 0.0 28-29 0.050118415969289294 30-31 0.0 32-33 0.0033251851712492236 34-35 0.0 36-37 2.6251461878283345E-4 38-39 0.0 40-41 0.0 42-43 0.0028876608066111683 44-45 0.0 46-47 1.7500974585522231E-4 48-49 2.8439083701473625E-4 50-51 5.906578922613753E-4 52-53 1.7500974585522231E-4 54-55 0.0 56-57 0.0018157261132479314 58-59 4.375243646380558E-5 60-61 0.0 62-63 0.0 64-65 5.469054557975697E-4 66-67 0.0 68-69 4.3752436463805576E-4 70-71 0.0 72-73 0.0037408333176553767 74-75 0.005097158848033349 76-77 7.656676381165977E-4 78-79 0.0011813157845227506 80-81 0.0017282212403203201 82-83 1.968859640871251E-4 84-85 3.281432734785419E-4 86-87 5.687816740294725E-4 88-89 0.0 90-91 0.0 92-93 0.00949427871264581 94-95 0.007350409325919337 96-97 0.008072324527572129 98-99 0.0012906968756822646 100-101 3.7189570994234744E-4 102-103 8.750487292761116E-5 104-105 8.094200745804032E-4 106-107 0.005534683212671406 108-109 0.008050448309340227 110-111 0.0016188401491608064 112-113 0.004922149102178128 114-115 5.687816740294725E-4 116-117 0.002909537024843071 118-119 0.0032158040800897097 120-121 0.015882134436361425 122-123 0.0017063450220884176 124-125 0.002297002914349793 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2285587.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.597774517093896 #Duplication Level Percentage of deduplicated Percentage of total 1 81.26012872223953 50.867032149847745 2 10.860228009816959 13.596522083254989 3 3.3546536947930536 6.299815667087745 4 1.3926114494848596 3.4869751001910614 5 0.7969733204730048 2.4944378105554383 6 0.4945704554330516 1.857540591120876 7 0.3237912276099551 1.41880271795787 8 0.24161026463885849 1.209941189350292 9 0.1749570568293544 0.985673015422048 >10 1.0108428808479615 11.498135117539803 >50 0.06474754960068481 2.7519754182348577 >100 0.024253591090328184 2.676147379318129 >500 2.1059238080855164E-4 0.07997452717113644 >1k 3.5098730134704197E-4 0.5332367512200332 >5k 7.01974602694084E-5 0.24379048172795587 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5548 0.24273851750119335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4847 0.21206805954006566 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3259 0.14258919043554238 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.375243646380558E-5 2 0.0 0.0 0.0 0.0 4.375243646380558E-5 3 0.0 0.0 0.0 0.0 4.375243646380558E-5 4 0.0 0.0 0.0 0.0 4.375243646380558E-5 5 0.0 0.0 0.0 0.0 4.375243646380558E-5 6 0.0 0.0 0.0 0.0 4.375243646380558E-5 7 0.0 0.0 0.0 0.0 4.375243646380558E-5 8 0.0 0.0 0.0 0.0 4.375243646380558E-5 9 0.0 0.0 0.0 8.750487292761116E-5 4.375243646380558E-5 10-11 0.0 0.0 0.0 1.0938109115951394E-4 4.375243646380558E-5 12-13 2.187621823190279E-5 0.0 0.0 1.3125730939141672E-4 8.750487292761116E-5 14-15 4.375243646380558E-5 0.0 0.0 1.3125730939141672E-4 8.750487292761116E-5 16-17 8.750487292761116E-5 0.0 0.0 1.3125730939141672E-4 8.750487292761116E-5 18-19 8.750487292761116E-5 0.0 0.0 1.3125730939141672E-4 8.750487292761116E-5 20-21 8.750487292761116E-5 0.0 2.187621823190279E-5 2.1876218231902788E-4 1.3125730939141672E-4 22-23 8.750487292761116E-5 0.0 4.375243646380558E-5 2.4063840055093066E-4 1.531335276233195E-4 24-25 8.750487292761116E-5 0.0 4.375243646380558E-5 2.6251461878283345E-4 1.7500974585522231E-4 26-27 8.750487292761116E-5 0.0 4.375243646380558E-5 3.5001949171044463E-4 1.7500974585522231E-4 28-29 1.3125730939141675E-4 0.0 4.375243646380558E-5 6.344103287251809E-4 1.7500974585522231E-4 30-31 1.7500974585522231E-4 0.0 4.375243646380558E-5 0.0018157261132479316 1.7500974585522231E-4 32-33 1.7500974585522231E-4 0.0 4.375243646380558E-5 0.004572129610467683 1.7500974585522231E-4 34-35 1.7500974585522231E-4 0.0 4.375243646380558E-5 0.007787933690557393 1.7500974585522231E-4 36-37 1.7500974585522231E-4 4.375243646380558E-5 4.375243646380558E-5 0.013081978502677867 1.7500974585522231E-4 38-39 2.1876218231902788E-4 4.375243646380558E-5 4.375243646380558E-5 0.02421697358271639 1.7500974585522231E-4 40-41 2.1876218231902788E-4 4.375243646380558E-5 4.375243646380558E-5 0.03786773375942373 1.7500974585522231E-4 42-43 2.4063840055093066E-4 4.375243646380558E-5 4.375243646380558E-5 0.04972464404111504 1.7500974585522231E-4 44-45 2.6251461878283345E-4 4.375243646380558E-5 4.375243646380558E-5 0.06414107185593898 1.7500974585522231E-4 46-47 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.08035134956577894 1.7500974585522231E-4 48-49 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.09866174422588159 1.7500974585522231E-4 50-51 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.12031920027546535 1.7500974585522231E-4 52-53 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.14081721675875825 1.7500974585522231E-4 54-55 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.16446540866744516 1.7500974585522231E-4 56-57 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.19253259665897643 1.7500974585522231E-4 58-59 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.22635323004549818 1.7500974585522231E-4 60-61 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.26465848816955995 1.7500974585522231E-4 62-63 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.30475759618863774 1.7500974585522231E-4 64-65 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.34406916035136703 1.7500974585522231E-4 66-67 3.0626705524663906E-4 4.375243646380558E-5 4.375243646380558E-5 0.3895060656190291 1.7500974585522231E-4 68-69 3.281432734785418E-4 4.375243646380558E-5 4.375243646380558E-5 0.4436934581794524 1.7500974585522231E-4 70-71 3.7189570994234744E-4 4.375243646380558E-5 4.375243646380558E-5 0.5047062308282293 1.7500974585522231E-4 72-73 3.937719281742502E-4 4.375243646380558E-5 4.375243646380558E-5 0.5698098562863719 2.1876218231902788E-4 74-75 3.937719281742502E-4 4.375243646380558E-5 4.375243646380558E-5 0.6377355138964301 2.6251461878283345E-4 76-77 3.937719281742502E-4 4.375243646380558E-5 4.375243646380558E-5 0.7163367660036568 2.6251461878283345E-4 78-79 3.937719281742502E-4 4.375243646380558E-5 4.375243646380558E-5 0.8065542899920239 2.6251461878283345E-4 80-81 5.250292375656669E-4 4.375243646380558E-5 4.375243646380558E-5 0.9089131150990971 2.6251461878283345E-4 82-83 5.250292375656669E-4 4.375243646380558E-5 4.375243646380558E-5 1.0226475736867597 3.0626705524663906E-4 84-85 5.250292375656669E-4 4.375243646380558E-5 4.375243646380558E-5 1.134872573216421 3.0626705524663906E-4 86-87 5.469054557975697E-4 4.375243646380558E-5 4.375243646380558E-5 1.2556074216382924 3.281432734785418E-4 88-89 5.687816740294725E-4 4.375243646380558E-5 4.375243646380558E-5 1.3947620458114263 3.5001949171044463E-4 90-91 5.906578922613753E-4 4.375243646380558E-5 4.375243646380558E-5 1.554764705959563 3.5001949171044463E-4 92-93 6.125341104932781E-4 4.375243646380558E-5 4.375243646380558E-5 1.7262961331159128 3.5001949171044463E-4 94-95 6.125341104932781E-4 4.375243646380558E-5 4.375243646380558E-5 1.901152745443512 4.3752436463805576E-4 96-97 6.125341104932781E-4 4.375243646380558E-5 4.375243646380558E-5 2.0853067505196696 4.5940058286995857E-4 98-99 6.125341104932781E-4 4.375243646380558E-5 4.375243646380558E-5 2.2851460040681015 5.250292375656669E-4 100-101 6.562865469570836E-4 4.375243646380558E-5 4.375243646380558E-5 2.525850033273728 5.250292375656669E-4 102-103 7.000389834208893E-4 4.375243646380558E-5 4.375243646380558E-5 2.789130319694678 5.250292375656669E-4 104-105 7.875438563485004E-4 4.375243646380558E-5 4.375243646380558E-5 3.0500042221101187 5.250292375656669E-4 106-107 9.4067738397182E-4 4.375243646380558E-5 4.375243646380558E-5 3.3153846254813315 5.250292375656669E-4 108-109 9.844298204356255E-4 4.375243646380558E-5 4.375243646380558E-5 3.599469195440821 5.250292375656669E-4 110-111 0.0010063060386675284 4.375243646380558E-5 4.375243646380558E-5 3.920655831521618 5.469054557975697E-4 112-113 0.0010063060386675284 4.375243646380558E-5 4.375243646380558E-5 4.282247842676739 5.687816740294725E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4555 0.0 49.17359 1 GTATCAA 7480 0.0 45.155838 1 ATCAACG 9205 0.0 35.679993 3 TCAACGC 9245 0.0 35.59309 4 TATCAAC 9470 0.0 35.019455 2 CAACGCA 9385 0.0 34.65319 5 AACGCAG 9600 0.0 34.117813 6 GTACATG 8545 0.0 31.580471 1 TACATGG 8740 0.0 29.633432 2 ACGCAGA 11695 0.0 27.700552 7 CGCAGAG 11700 0.0 27.434223 8 ACATGGG 9015 0.0 26.861988 3 GTGGTAT 2960 0.0 25.15653 1 TGGTATC 2850 0.0 23.606834 2 GCAGAGT 13290 0.0 23.256865 9 GAGTACT 7925 0.0 21.380152 12-13 GTACTTT 8065 0.0 20.972094 14-15 AGTACTT 8055 0.0 20.628443 12-13 AGAGTAC 12465 0.0 20.554802 10-11 ATACAGT 2390 0.0 20.431757 6 >>END_MODULE