FastQCFastQC Report
Thu 26 May 2016
SRR1364624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796201
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA75550.037.848731
ATCAACG85950.032.505433
TCAACGC86500.032.505354
CAACGCA88500.031.7707635
GTACATG128650.031.1637541
TACATGG126350.030.5040822
TATCAAC93200.030.2964212
AACGCAG90350.030.1047746
ACATGGG129500.029.4860883
GGTATCA52150.028.7866211
TAGGACG8500.025.9305484
ACGCAGA105350.025.4192717
TACCTGG27100.025.0590442
TAGCCCT28000.024.0408234
CGCAGAG103250.023.9723458
GTGTAGC25200.023.8762071
CATGGGT34000.023.477664
GTATAGG8200.023.2477321
GTACCTG31100.022.4114511
GAGTACA103500.022.3899841
Produced by FastQC (version 0.11.5)