##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364624_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2796201 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.62046326426462 31.0 26.0 31.0 10.0 34.0 2 26.145858255540286 31.0 25.0 31.0 10.0 34.0 3 26.85179677712725 31.0 26.0 31.0 10.0 34.0 4 30.25488224916592 35.0 30.0 37.0 10.0 37.0 5 30.52717347572653 35.0 31.0 37.0 10.0 37.0 6 30.51331038076304 35.0 31.0 37.0 8.0 37.0 7 30.442783977260575 35.0 31.0 37.0 8.0 37.0 8 30.20778120027852 35.0 30.0 37.0 8.0 37.0 9 31.150135129770714 37.0 30.0 39.0 8.0 39.0 10-11 31.10768110017842 37.0 30.0 39.0 7.0 39.0 12-13 31.082398225306406 37.0 30.0 39.0 7.0 39.0 14-15 31.73291977221952 38.0 29.5 40.0 2.0 41.0 16-17 32.1230911154098 38.0 31.0 40.0 2.0 41.0 18-19 31.943741347635594 38.0 30.0 40.0 2.0 41.0 20-21 31.86099675953195 38.0 30.0 40.0 2.0 41.0 22-23 31.928013758667564 38.0 30.0 40.0 2.0 41.0 24-25 32.02007491593058 38.0 30.0 40.0 2.0 41.0 26-27 31.83108277981447 38.0 30.0 40.0 2.0 41.0 28-29 31.73576577649461 38.0 30.0 40.0 2.0 41.0 30-31 31.599888563089706 38.0 30.0 40.0 2.0 41.0 32-33 31.515905687752777 38.0 30.0 40.0 2.0 41.0 34-35 31.569349628299253 38.0 30.0 40.0 2.0 41.0 36-37 31.50785333386262 38.0 30.0 40.0 2.0 41.0 38-39 31.21536166391472 37.0 30.0 40.0 2.0 41.0 40-41 31.075305208745725 37.0 29.0 40.0 2.0 41.0 42-43 30.576416359195925 37.0 27.5 40.0 2.0 41.0 44-45 30.438999378084766 36.0 28.0 40.0 2.0 41.0 46-47 30.407279734182197 36.5 28.0 40.0 2.0 41.0 48-49 30.34216495881376 36.0 28.0 40.0 2.0 41.0 50-51 30.106320682955193 36.0 28.0 40.0 2.0 41.0 52-53 30.002501250804215 36.0 27.5 40.0 2.0 41.0 54-55 29.821275187298767 36.0 27.5 40.0 2.0 41.0 56-57 29.594241079235722 35.0 27.0 39.5 2.0 41.0 58-59 29.383399119018982 35.0 26.5 39.0 2.0 41.0 60-61 29.088243119861552 35.0 26.0 39.0 2.0 40.0 62-63 28.903433086534193 34.5 26.0 39.0 2.0 40.0 64-65 28.67368440251613 34.0 26.0 38.5 2.0 40.0 66-67 28.414124556854105 34.0 26.0 38.0 2.0 40.0 68-69 28.15641257549082 34.0 26.0 38.0 2.0 40.0 70-71 27.718260239517832 34.0 25.0 37.0 2.0 40.0 72-73 27.47587387315862 33.5 25.0 37.0 2.0 39.0 74-75 27.44673290653998 34.0 25.0 36.0 2.0 39.0 76-77 27.18933671077294 33.0 25.0 36.0 2.0 39.0 78-79 26.89387315146515 33.0 24.0 35.5 2.0 38.5 80-81 26.484270265263476 33.0 23.0 35.0 2.0 37.0 82-83 26.59685730746824 33.0 24.5 35.0 2.0 37.0 84-85 26.43303664507666 33.0 24.0 35.0 2.0 36.5 86-87 26.30768621425999 33.0 24.0 35.0 2.0 36.0 88-89 26.129415946850745 33.0 24.0 35.0 2.0 36.0 90-91 25.993806239251043 33.0 23.0 35.0 2.0 35.5 92-93 25.87141053164633 33.0 23.0 35.0 2.0 35.0 94-95 25.767548720567653 33.0 23.0 35.0 2.0 35.0 96-97 25.641848708301012 33.0 21.0 35.0 2.0 35.0 98-99 25.55007365350345 33.0 20.0 35.0 2.0 35.0 100-101 25.445211020237814 33.0 20.0 35.0 2.0 35.0 102-103 24.282584478011415 30.5 18.5 34.0 2.0 35.0 104-105 25.140133524020627 32.5 19.0 35.0 2.0 35.0 106-107 25.302353979560124 33.0 19.0 35.0 2.0 35.0 108-109 25.26221255195889 33.0 19.0 35.0 2.0 35.0 110-111 25.18928592758532 33.0 18.0 35.0 2.0 35.0 112-113 25.09740841234232 33.0 17.5 35.0 2.0 35.0 114-115 24.990595990774626 33.0 16.0 35.0 2.0 35.0 116-117 24.877790080183793 33.0 8.5 35.0 2.0 35.0 118-119 24.749799638867163 33.0 3.5 35.0 2.0 35.0 120-121 24.56493649776965 33.0 2.0 35.0 2.0 35.0 122-123 24.348378925549344 33.0 2.0 35.0 2.0 35.0 124-125 23.397566555480097 31.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.6655923245657682 1101 2 1.8985906828390036 1101 3 1.4275592299159037 1101 4 1.5935117830735557 1101 5 1.443028133534085 1101 6 1.240008783016016 1101 7 1.2934282962040449 1101 8 1.4249414298093015 1101 9 1.8487407662943909 1101 10-11 1.7310868105249355 1101 12-13 1.6936330200691714 1101 14-15 2.117217719516212 1101 16-17 1.7241679562213648 1101 18-19 1.7702756104894348 1101 20-21 1.9355874798089232 1101 22-23 1.6833704847627402 1101 24-25 1.6291337826352077 1101 26-27 1.9259979815854074 1101 28-29 1.9013060679141134 1101 30-31 1.9575353747699928 1101 32-33 2.0130028136841425 1101 34-35 1.8219763303551488 1101 36-37 1.6961538710241193 1101 38-39 1.5947427944829222 1101 40-41 1.696449273272144 1101 42-43 1.917756195698562 1101 44-45 1.7634601439070465 1101 46-47 1.4952825957086766 1101 48-49 1.384279550791657 1101 50-51 1.3217373055226638 1101 52-53 1.298467124351994 1101 54-55 1.246252500947346 1101 56-57 1.3456120712416855 1101 58-59 1.1888631307852684 1101 60-61 1.321969579557905 1101 62-63 1.2413438014252307 1101 64-65 1.143994712100973 1101 66-67 1.1639341421501364 1101 68-69 1.0485844335208405 1101 70-71 0.984141103433803 1101 72-73 0.8860740383566501 1101 74-75 0.9755247412244117 1101 76-77 1.0841794838563352 1101 78-79 1.1921108386471317 1101 80-81 1.2696179822645455 1101 82-83 1.2851714462984312 1101 84-85 1.295645109448099 1101 86-87 1.1573804419253086 1101 88-89 1.1676214842202626 1101 90-91 1.2181547893861513 1101 92-93 1.2091879103182421 1101 94-95 1.1660120344123541 1101 96-97 1.177334487444238 1101 98-99 1.1766488187200075 1101 100-101 1.1869346866075077 1101 102-103 1.0318108816262566 1101 104-105 1.139716847600372 1101 106-107 1.2269848515329684 1101 108-109 1.1820007168748994 1101 110-111 1.156613713897503 1101 112-113 1.105799855765845 1101 114-115 1.124060900245766 1101 116-117 1.1687523693689386 1101 118-119 1.0834391379198145 1101 120-121 1.1125474191766571 1101 122-123 1.019672460324049 1101 124-125 0.9538449902225139 1102 1 1.1755250625322518 1102 2 1.6242748911968015 1102 3 1.228810221335614 1102 4 1.2358667782838388 1102 5 1.1629142103961492 1102 6 0.853378270979924 1102 7 0.9411812498622538 1102 8 0.9278827274618244 1102 9 1.5579931585772826 1102 10-11 1.554065086845199 1102 12-13 1.6988162138312077 1102 14-15 1.797370269396211 1102 16-17 1.528995526444774 1102 18-19 1.3715922673706658 1102 20-21 1.6790387019590511 1102 22-23 1.6703172210605501 1102 24-25 1.3789833958527566 1102 26-27 1.7249553170612408 1102 28-29 1.4498670930163442 1102 30-31 1.3932764416921124 1102 32-33 1.67250730983692 1102 34-35 1.569739069454677 1102 36-37 1.4740274431222211 1102 38-39 1.6950940403241113 1102 40-41 1.6517632829339846 1102 42-43 1.8658392792456304 1102 44-45 1.3914586091378247 1102 46-47 1.3204602787221837 1102 48-49 1.246433068915323 1102 50-51 1.3965889788331296 1102 52-53 1.3560209700304355 1102 54-55 1.3083313407030168 1102 56-57 1.2893666420728316 1102 58-59 1.29307756585629 1102 60-61 1.2224029716038878 1102 62-63 1.000423918438507 1102 64-65 0.8877781242328595 1102 66-67 0.9131690751456141 1102 68-69 0.8837298760803023 1102 70-71 1.1095343236680328 1102 72-73 0.7336384935135776 1102 74-75 0.9995152956984157 1102 76-77 0.7711012415276102 1102 78-79 0.8085488350007637 1102 80-81 1.0043578440323095 1102 82-83 1.013465277350214 1102 84-85 0.8337815494015395 1102 86-87 0.8574358069222718 1102 88-89 0.8841112404082665 1102 90-91 0.9190748783821618 1102 92-93 1.0025580163100223 1102 94-95 0.983607802981517 1102 96-97 0.7931189193221151 1102 98-99 0.859194424026807 1102 100-101 0.8472900011321904 1102 102-103 0.7346502046797276 1102 104-105 0.9332994006735156 1102 106-107 0.8048424579110147 1102 108-109 0.979069462175314 1102 110-111 0.9094790456239643 1102 112-113 0.8409777447886455 1102 114-115 0.9137738262987298 1102 116-117 0.9515142844313047 1102 118-119 1.0095724465320366 1102 120-121 0.9576343550974649 1102 122-123 0.902911544406745 1102 124-125 0.9255416312410212 1103 1 1.217376432256568 1103 2 1.4616077616877057 1103 3 1.1462972762113708 1103 4 0.9338594885155516 1103 5 0.9512002470185585 1103 6 0.9451202941799011 1103 7 1.0322235131617425 1103 8 1.1340746913601158 1103 9 1.5765829355450833 1103 10-11 1.6005581876002068 1103 12-13 1.5786883536096283 1103 14-15 1.8315069236239836 1103 16-17 1.4963742472640007 1103 18-19 1.3730249093331182 1103 20-21 1.7180827877006024 1103 22-23 1.5889168334300123 1103 24-25 1.4551287862314268 1103 26-27 1.6767998887873539 1103 28-29 1.58910369228159 1103 30-31 1.6540357925556002 1103 32-33 1.8386080069933293 1103 34-35 1.5502617921252764 1103 36-37 1.5474342526914917 1103 38-39 1.532524686278574 1103 40-41 1.6828631122608328 1103 42-43 1.9456336036403137 1103 44-45 1.6650585107838083 1103 46-47 1.4214283425944387 1103 48-49 1.2759407202792623 1103 50-51 1.3196045419098716 1103 52-53 1.128611441261885 1103 54-55 1.2513808937707935 1103 56-57 1.2446602577400476 1103 58-59 1.2152491646876094 1103 60-61 1.2569175656042937 1103 62-63 1.1192597250860814 1103 64-65 1.0756946101017029 1103 66-67 1.0542566102203956 1103 68-69 1.0650288722907781 1103 70-71 1.213468773608735 1103 72-73 1.0864308797555857 1103 74-75 1.0882171673174952 1103 76-77 1.0649450032794654 1103 78-79 1.0513256003107259 1103 80-81 1.1726736761362169 1103 82-83 1.1305363525910366 1103 84-85 1.206101836363846 1103 86-87 1.1082447687088397 1103 88-89 1.1055169796544106 1103 90-91 1.0613475793570224 1103 92-93 1.0434405989707862 1103 94-95 1.0872952107746272 1103 96-97 1.095648931761005 1103 98-99 0.9747666129822292 1103 100-101 0.8976349633816056 1103 102-103 0.7841601702558236 1103 104-105 0.9095427111540069 1103 106-107 0.9686872321306907 1103 108-109 1.0626358077450426 1103 110-111 1.0005914584794162 1103 112-113 1.0435799317191297 1103 114-115 1.0111576094570545 1103 116-117 1.0151312533919352 1103 118-119 1.005221727679885 1103 120-121 0.9455077387649133 1103 122-123 0.8323228666897116 1103 124-125 0.7228474543773586 1104 1 1.2239860701236935 1104 2 1.6389815854687129 1104 3 1.1002422434198103 1104 4 1.2514257844950194 1104 5 0.9394152455928371 1104 6 0.8704624669771626 1104 7 0.8752702767773677 1104 8 1.1474479448531305 1104 9 1.5745356016414647 1104 10-11 1.6255040102406397 1104 12-13 1.5608262207325154 1104 14-15 1.6778378332333403 1104 16-17 1.3985762717863892 1104 18-19 1.377366939558378 1104 20-21 1.6017975011308963 1104 22-23 1.4988662203704237 1104 24-25 1.4196597243551707 1104 26-27 1.6341530396285258 1104 28-29 1.7120185767982647 1104 30-31 1.6620704375513249 1104 32-33 1.722495232542233 1104 34-35 1.6330447975430147 1104 36-37 1.5016016322181507 1104 38-39 1.613130617273729 1104 40-41 1.6770048288276982 1104 42-43 1.8479717844216133 1104 44-45 1.6871849721467953 1104 46-47 1.4846269453888503 1104 48-49 1.3465124339946861 1104 50-51 1.3013025744162903 1104 52-53 1.203598609781988 1104 54-55 1.282218504264094 1104 56-57 1.24808127285268 1104 58-59 1.256642093116465 1104 60-61 1.3043819226045805 1104 62-63 1.1954808542563136 1104 64-65 1.2528160814447311 1104 66-67 1.1973012352560382 1104 68-69 1.1560625192825071 1104 70-71 1.147429423737048 1104 72-73 1.1773255190484306 1104 74-75 1.1610956959744705 1104 76-77 1.1550832981183596 1104 78-79 1.2160867922126357 1104 80-81 1.3341749592842085 1104 82-83 1.2944435382197774 1104 84-85 1.3881776846672373 1104 86-87 1.2834572009871437 1104 88-89 1.3228672790556075 1104 90-91 1.3292232565740214 1104 92-93 1.3025994241775187 1104 94-95 1.2647723992548094 1104 96-97 1.2684846888183188 1104 98-99 1.3411388684712549 1104 100-101 1.2901057361218413 1104 102-103 1.296077050780486 1104 104-105 1.3989895317984313 1104 106-107 1.4051357636391053 1104 108-109 1.3247216360883591 1104 110-111 1.31681686480961 1104 112-113 1.3800719476871919 1104 114-115 1.4030060554222636 1104 116-117 1.3690384390206773 1104 118-119 1.398570031073099 1104 120-121 1.435507025904922 1104 122-123 1.437690702446769 1104 124-125 1.1958314863134873 1105 1 1.340471913410095 1105 2 1.6541327164488955 1105 3 1.1451657326660474 1105 4 1.2165899267516416 1105 5 1.0069015114677065 1105 6 0.9316945347692496 1105 7 0.8586524301992249 1105 8 1.0764375135904807 1105 9 1.3127803026045228 1105 10-11 1.2625119726969913 1105 12-13 1.2998817911157374 1105 14-15 1.647673301750494 1105 16-17 1.216384427743776 1105 18-19 1.4132239600872794 1105 20-21 1.340860856038475 1105 22-23 1.4659486362419543 1105 24-25 1.1809036913487887 1105 26-27 1.5366458111416144 1105 28-29 1.5572629552595956 1105 30-31 1.492450266736622 1105 32-33 1.6737870743606997 1105 34-35 1.432285917624867 1105 36-37 1.1214867548800136 1105 38-39 1.374040850587047 1105 40-41 1.383757903714823 1105 42-43 1.6037258824176526 1105 44-45 1.3647000963655636 1105 46-47 0.8701294364366454 1105 48-49 0.9322315016310831 1105 50-51 1.1202780198022921 1105 52-53 0.9406827493696284 1105 54-55 1.0062890876585904 1105 56-57 1.0083352151582332 1105 58-59 1.039317189310946 1105 60-61 0.9704811530487412 1105 62-63 0.9611352547367318 1105 64-65 0.7071601796631839 1105 66-67 0.6577993892116858 1105 68-69 0.581558727107069 1105 70-71 0.894107878397751 1105 72-73 0.7127835270998517 1105 74-75 0.708512457167501 1105 76-77 0.7958011424723601 1105 78-79 0.8339989271740471 1105 80-81 0.870288591098447 1105 82-83 0.8561495167344901 1105 84-85 0.7128639683353057 1105 86-87 0.8031860771242414 1105 88-89 0.9401524261831185 1105 90-91 0.692574383105903 1105 92-93 0.766565083304318 1105 94-95 0.8063680599385137 1105 96-97 0.776857612018425 1105 98-99 0.8241004907661278 1105 100-101 0.8635608416577476 1105 102-103 0.7467472427246165 1105 104-105 0.8407281497117332 1105 106-107 0.8236379099436775 1105 108-109 0.8583468890854 1105 110-111 0.8183700442317772 1105 112-113 0.7732671057130389 1105 114-115 0.9152883111550096 1105 116-117 0.8583857066526264 1105 118-119 0.8506091872306563 1105 120-121 0.7664669133629367 1105 122-123 0.753955798863668 1105 124-125 0.6582283413656427 1106 1 0.319312138050865 1106 2 -2.2970071225144792 1106 3 -0.37531404159419424 1106 4 0.20155971306644815 1106 5 0.4235166986740708 1106 6 0.3615115741200192 1106 7 0.5684364182339507 1106 8 0.5850092193629344 1106 9 0.9176492220896435 1106 10-11 1.1130694426636083 1106 12-13 1.138888633197503 1106 14-15 1.216152127701129 1106 16-17 0.8678079332009574 1106 18-19 0.9937727518833128 1106 20-21 1.1112401219292067 1106 22-23 0.9371371850062715 1106 24-25 1.0089550674924297 1106 26-27 0.9326898918694262 1106 28-29 1.1305873793192731 1106 30-31 1.2280657106605695 1106 32-33 1.2468227447971323 1106 34-35 1.0175309670108064 1106 36-37 0.8786385719940633 1106 38-39 1.2407732993465608 1106 40-41 1.2703488852002494 1106 42-43 1.3489305116625054 1106 44-45 1.1270926227070213 1106 46-47 0.9971070311731616 1106 48-49 0.8823424847649939 1106 50-51 0.9009585618112474 1106 52-53 0.8788040982273628 1106 54-55 0.8208816257626914 1106 56-57 0.7785268260739677 1106 58-59 0.8592611407355157 1106 60-61 0.9079424092992561 1106 62-63 0.7942072198025336 1106 64-65 0.7519087810245608 1106 66-67 0.753722366208418 1106 68-69 0.6439435154309692 1106 70-71 0.6941842819303226 1106 72-73 0.5258065677178969 1106 74-75 0.5319340625711106 1106 76-77 0.5593274332724221 1106 78-79 0.6548639280669768 1106 80-81 0.7682256420573204 1106 82-83 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-2.235532076072978 2216 76-77 -2.176365687613117 2216 78-79 -2.3161407305654294 2216 80-81 -2.135422126842659 2216 82-83 -2.2197487000473686 2216 84-85 -2.1014868761029177 2216 86-87 -2.252858124782154 2216 88-89 -2.293481998922733 2216 90-91 -2.1697263738196533 2216 92-93 -2.231328932363656 2216 94-95 -2.362502828525834 2216 96-97 -2.3194240270145876 2216 98-99 -2.35737948291262 2216 100-101 -2.4692743137320328 2216 102-103 -2.163018703044507 2216 104-105 -2.1834410595716918 2216 106-107 -2.239818680232304 2216 108-109 -2.3670127201089954 2216 110-111 -2.193201830394102 2216 112-113 -2.4756846708446965 2216 114-115 -2.3272745875208365 2216 116-117 -2.1776086728926813 2216 118-119 -2.3079943491916417 2216 120-121 -2.460442346623875 2216 122-123 -2.2290034698069654 2216 124-125 -2.1204516063459593 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 244394.0 3 32472.0 4 19097.0 5 15665.0 6 15211.0 7 16823.0 8 18749.0 9 20185.0 10 21224.0 11 22264.0 12 23966.0 13 24510.0 14 23737.0 15 21097.0 16 17758.0 17 15682.0 18 14092.0 19 13127.0 20 13017.0 21 13998.0 22 17057.0 23 24026.0 24 34456.0 25 45409.0 26 51550.0 27 55729.0 28 64498.0 29 73166.0 30 85378.0 31 98731.0 32 118136.0 33 148890.0 34 194574.0 35 264752.0 36 378862.0 37 421841.0 38 111860.0 39 218.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.92908878094374 18.571619026730403 13.836833666271728 23.66245852605413 2 13.42630967575397 20.69821886965625 41.84247321851113 24.032998236078644 3 19.502590569494842 25.36376301920795 27.99339551847978 27.14025089281743 4 11.902295738125893 16.805154561528724 37.39815150221827 33.894398198127114 5 13.33380213522519 39.067868760823856 33.421006462649345 14.177322641301615 6 31.696670538133954 36.878055949871666 17.59259259259259 13.832680919401785 7 28.62151270468196 32.74158681547609 20.276879590032102 18.36002088980984 8 24.96849945599743 37.605915651649134 19.23360860771179 18.19197628464164 9 25.489742148930922 17.83204704047522 19.731526980613864 36.94668382997999 10-11 24.094990029843316 28.0777161849623 27.312194392536753 20.515099392657632 12-13 24.961375752266 25.12696070116019 27.480563382905522 22.43110016366829 14-15 21.902942190810187 26.661435308977065 25.282936726549586 26.15268577366316 16-17 22.131625114965328 29.767687197586334 25.733749701112536 22.366937986335806 18-19 22.11428023730675 28.562499048246938 27.53895917180912 21.784261542637193 20-21 23.118294498624795 27.386544470812566 27.64771965703805 21.847441373524585 22-23 22.556155207821348 27.55235021619556 27.275186992895495 22.616307583087597 24-25 22.402891925638553 27.4046361326228 27.63289701582041 22.559574925918234 26-27 22.254131964000887 27.65080803688106 27.46307215704636 22.631987842071695 28-29 22.096135357689402 27.856815685779967 27.536420621433148 22.510628335097483 30-31 22.350704809554202 27.557025565420094 27.580540708599198 22.511728916426502 32-33 22.09156118627281 27.464665083326047 27.597730911405073 22.846042818996075 34-35 22.540768916531913 27.620082428815017 27.48814546269902 22.351003191954046 36-37 22.57350229783387 27.300659038919633 27.389201891134917 22.73663677211158 38-39 22.27460599751231 27.6357199302529 27.43410330675269 22.655570765482103 40-41 22.73505759072241 27.27191278607215 27.590722410567736 22.402307212637705 42-43 22.253827509007326 27.340246571441995 27.630127058273384 22.775798861277295 44-45 22.457058237361352 27.65800424496101 27.376863798156386 22.50807371952125 46-47 22.629610452690713 27.6664145539315 26.917598537543157 22.78637645583463 48-49 22.494614804733278 27.541537241981224 27.110488786059584 22.853359167225918 50-51 22.540888176890263 27.810730800038403 27.01189962729929 22.636481395772044 52-53 22.680462052507337 27.72227261269915 26.96316690024638 22.634098434547127 54-55 22.622038435736975 27.62823182533477 27.089196453601527 22.66053328532673 56-57 22.61602979488076 27.845807336373195 26.861087387043987 22.677075481702058 58-59 22.592411323399027 27.5802848918972 26.9608814911956 22.866422293508172 60-61 22.50582984378526 27.558110378375666 27.23233763302969 22.70372214480938 62-63 22.513639379550376 27.596827730584533 27.239056485342534 22.65047640452255 64-65 22.49992210395952 27.677251097080674 27.09286962924708 22.72995716971273 66-67 22.472008690363197 27.82669121081919 26.901451145265465 22.799848953552143 68-69 22.800481950750992 27.505016739175943 27.090158775519924 22.604342534553144 70-71 22.649993437498935 27.416356760835427 27.107623764024996 22.82602603764064 72-73 22.615205350044494 27.55252506826384 26.956092564205065 22.876177017486604 74-75 22.500802460748346 27.74818862276253 26.936562009315168 22.814446907173956 76-77 22.672982906865958 27.5181713367296 27.01333289881726 22.79551285758718 78-79 22.685867233584432 27.617847666755786 26.998975171501638 22.697309928158145 80-81 22.539626936932873 27.767904025697064 27.02393411831453 22.66853491905553 82-83 22.55408877602939 27.82496740086433 27.093414320910885 22.527529502195392 84-85 22.625971629702 27.697910906606076 26.985895790297025 22.690221673394895 86-87 22.53177598568202 27.71620396599552 27.001917100236778 22.750102948085686 88-89 22.763600477140596 27.67859637559419 26.937380670074475 22.620422477190747 90-91 22.580229219433633 27.832895497722294 27.011588839140764 22.57528644370331 92-93 22.44450676721407 27.729996987732804 27.151989117154983 22.673507127898144 94-95 22.425613550505403 27.77934214273815 27.132142863540203 22.66290144321624 96-97 22.60527319717803 27.613377679628464 27.10287588231396 22.678473240879544 98-99 22.499137661466843 27.596892644135902 26.995655533097146 22.90831416130011 100-101 22.3370384701677 27.69307020576858 27.237509734064204 22.732381589999516 102-103 22.311082832395225 27.459433238645016 27.353549271423006 22.87593465753675 104-105 22.211234364908712 27.587054000275383 27.24259273086094 22.959118903954966 106-107 22.171109449000923 27.66503820058049 27.146648435582062 23.01720391483653 108-109 22.20347788619048 27.64380651618785 27.033284389125967 23.119431208495698 110-111 22.138646648633824 27.715257233011137 27.182883693882797 22.963212424472246 112-113 22.27702556701053 27.711257286516545 27.1750076219635 22.836709524509423 114-115 22.08184239188422 27.847068612785346 27.104492051136813 22.96659694419362 116-117 22.089262642145417 27.821429600835412 27.141251268184476 22.948056488834695 118-119 21.962665754017557 27.846495951879717 27.159270455890784 23.03156783821194 120-121 21.91910794109619 27.939109462464707 27.198578862821783 22.943203733617324 122-123 21.98538388560766 27.86468268880789 27.14218422448639 23.00774920109806 124-125 22.00046726504043 27.7744846433613 27.158768962501533 23.06627912909674 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4369.0 1 3181.0 2 1588.5 3 1066.5 4 877.5 5 767.5 6 720.5 7 790.0 8 920.0 9 991.5 10 1016.0 11 1024.0 12 1091.5 13 1219.0 14 1319.5 15 1479.5 16 1656.0 17 1859.5 18 2200.5 19 2601.5 20 3147.0 21 4006.0 22 5143.5 23 6621.0 24 8556.0 25 11175.5 26 15074.0 27 19759.0 28 24093.0 29 28353.0 30 33333.0 31 39328.5 32 46322.5 33 52281.0 34 57978.5 35 64218.0 36 69788.0 37 74950.0 38 78607.5 39 81386.5 40 84637.5 41 88259.5 42 90457.5 43 93127.0 44 96445.5 45 98549.5 46 100924.5 47 103378.5 48 105323.5 49 105240.5 50 104072.5 51 101519.5 52 97994.0 53 95007.0 54 91992.0 55 86145.5 56 78900.5 57 72719.0 58 66085.0 59 58310.0 60 48829.5 61 40230.5 62 32743.0 63 24909.5 64 18512.5 65 14378.5 66 11066.5 67 8376.0 68 6426.5 69 5039.5 70 3763.5 71 2663.0 72 1976.5 73 1501.5 74 1125.0 75 862.0 76 619.0 77 390.0 78 280.0 79 207.5 80 141.0 81 102.5 82 65.0 83 45.0 84 40.0 85 28.5 86 23.0 87 21.5 88 17.0 89 12.5 90 9.5 91 7.5 92 5.5 93 4.5 94 3.0 95 2.0 96 1.5 97 1.0 98 1.5 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21082175423011434 2 0.24894490775162442 3 0.3003003003003003 4 0.28578060017859946 5 0.320220184457412 6 0.23463978447901276 7 0.22616399893999037 8 0.20645869163196778 9 0.2057434354683372 10-11 0.1472891254956278 12-13 0.17468343656267915 14-15 0.1909197514770934 16-17 0.1644552734227618 18-19 0.18948923914983223 20-21 0.18462549723714425 22-23 0.2069772523506 24-25 0.19792926188067309 26-27 0.24712100453436642 28-29 0.17736564717629383 30-31 0.15674838825964227 32-33 0.1958729004102352 34-35 0.15512118048738271 36-37 0.15494236644647508 38-39 0.1881302524389341 40-41 0.20817530642468118 42-43 0.27315632889051966 44-45 0.17493377621994985 46-47 0.1595200058937108 48-49 0.161630011576421 50-51 0.1675487563304641 52-53 0.1866639773034914 54-55 0.17589937204085115 56-57 0.17341385687223487 58-59 0.1487554006310705 60-61 0.1620412838705086 62-63 0.14997133610924251 64-65 0.14369496327338413 66-67 0.13169654112848111 68-69 0.13588078968572 70-71 0.13663180865753213 72-73 0.11363632299680888 74-75 0.11549598902224839 76-77 0.1367033342738952 78-79 0.14399894714292713 80-81 0.15331515867421547 82-83 0.15494236644647508 84-85 0.14251479060339367 86-87 0.16971240622544662 88-89 0.16347179619776975 90-91 0.1519025277510451 92-93 0.1532078702496709 94-95 0.17402182461132085 96-97 0.16222009791141626 98-99 0.1564265229860085 100-101 0.136488757424806 102-103 0.17659674680039095 104-105 0.13319857907210533 106-107 0.13949283331205445 108-109 0.15186676494286355 110-111 0.1258493219908011 112-113 0.11749870628041403 114-115 0.11526353076906845 116-117 0.11910803264858283 118-119 0.10254985246053484 120-121 0.09525423959150289 122-123 0.12379296052036316 124-125 0.11993057723675801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2796201.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.13164644427768 #Duplication Level Percentage of deduplicated Percentage of total 1 82.8399121737681 57.268595198719495 2 10.46506684418318 14.469346021756085 3 3.144757825075242 6.522068583479316 4 1.326325257504872 3.667641950877694 5 0.6560029015839941 2.267528032936248 6 0.3954705673580762 1.6403718865029855 7 0.2525554689929367 1.2221702772991903 8 0.16198199172380226 0.8958465425751846 9 0.11948095548829256 0.7433923654477007 >10 0.588712881882405 7.191061809493306 >50 0.03400569780291502 1.5857870248086678 >100 0.014405326279271298 1.7325864888365436 >500 0.0010576866856242951 0.47527754297321007 >1k 2.64421671339606E-4 0.31832627429436416 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.576280818152915E-5 0.0 0.0 0.0 3 0.0 3.576280818152915E-5 0.0 0.0 0.0 4 0.0 3.576280818152915E-5 0.0 0.0 0.0 5 3.576280818152915E-5 7.15256163630583E-5 0.0 0.0 0.0 6 3.576280818152915E-5 7.15256163630583E-5 0.0 0.0 0.0 7 3.576280818152915E-5 7.15256163630583E-5 0.0 0.0 0.0 8 3.576280818152915E-5 7.15256163630583E-5 0.0 0.0 0.0 9 3.576280818152915E-5 7.15256163630583E-5 0.0 3.576280818152915E-5 0.0 10-11 3.576280818152915E-5 7.15256163630583E-5 0.0 3.576280818152915E-5 0.0 12-13 3.576280818152915E-5 7.15256163630583E-5 0.0 3.576280818152915E-5 0.0 14-15 7.15256163630583E-5 7.15256163630583E-5 0.0 7.15256163630583E-5 0.0 16-17 1.2516982863535205E-4 7.15256163630583E-5 0.0 7.15256163630583E-5 0.0 18-19 1.430512327261166E-4 1.0728842454458747E-4 0.0 1.609326368168812E-4 0.0 20-21 1.7881404090764577E-4 1.430512327261166E-4 0.0 2.1457684908917493E-4 1.7881404090764577E-5 22-23 1.7881404090764577E-4 1.7881404090764577E-4 0.0 3.5762808181529155E-4 1.0728842454458747E-4 24-25 2.1457684908917493E-4 1.7881404090764577E-4 0.0 5.364421227229373E-4 1.7881404090764577E-4 26-27 2.1457684908917493E-4 1.7881404090764577E-4 0.0 6.79493355449054E-4 1.7881404090764577E-4 28-29 3.218652736337624E-4 1.7881404090764577E-4 0.0 0.0011086470536274037 1.7881404090764577E-4 30-31 3.933908899968207E-4 1.7881404090764577E-4 0.0 0.0020742428745286912 1.7881404090764577E-4 32-33 4.112722940875853E-4 2.1457684908917493E-4 0.0 0.004935267529051024 2.1457684908917493E-4 34-35 4.2915369817834987E-4 2.1457684908917493E-4 0.0 0.008279090094024 2.1457684908917493E-4 36-37 4.2915369817834987E-4 2.3245825317993953E-4 0.0 0.014394530293065484 2.1457684908917493E-4 38-39 4.8279791045064357E-4 2.503396572707041E-4 0.0 0.02465845624116435 2.1457684908917493E-4 40-41 5.006793145414082E-4 2.503396572707041E-4 0.0 0.03889205389741296 2.1457684908917493E-4 42-43 5.185607186321728E-4 2.503396572707041E-4 0.0 0.05142691816503892 2.1457684908917493E-4 44-45 5.722049309044665E-4 2.503396572707041E-4 0.0 0.06707314674445793 2.1457684908917493E-4 46-47 6.973747595398186E-4 2.503396572707041E-4 0.0 0.0863671817583929 2.503396572707041E-4 48-49 7.152561636305831E-4 2.503396572707041E-4 0.0 0.10789639228367345 2.861024654522332E-4 50-51 7.152561636305831E-4 2.503396572707041E-4 0.0 0.1318932365734795 2.861024654522332E-4 52-53 7.152561636305831E-4 2.861024654522332E-4 0.0 0.15621194613691936 2.861024654522332E-4 54-55 7.152561636305831E-4 3.933908899968207E-4 0.0 0.18323074771806463 2.861024654522332E-4 56-57 7.867817799936414E-4 3.933908899968207E-4 0.0 0.21547091929371315 2.861024654522332E-4 58-59 7.867817799936414E-4 4.2915369817834987E-4 0.0 0.2505899969279748 2.861024654522332E-4 60-61 7.867817799936414E-4 4.64916506359879E-4 0.0 0.2924324825003639 2.861024654522332E-4 62-63 7.867817799936414E-4 4.64916506359879E-4 0.0 0.3347935287913852 2.861024654522332E-4 64-65 7.867817799936414E-4 5.364421227229373E-4 0.0 0.3783526291564877 3.2186527363376236E-4 66-67 7.867817799936414E-4 5.364421227229373E-4 0.0 0.4297974287256174 3.2186527363376236E-4 68-69 7.867817799936414E-4 5.364421227229373E-4 0.0 0.49098759352421373 3.2186527363376236E-4 70-71 7.867817799936414E-4 5.543235268137018E-4 0.0 0.562298633038183 3.5762808181529155E-4 72-73 8.046631840844059E-4 5.722049309044665E-4 0.0 0.6367389182680359 5.006793145414082E-4 74-75 8.225445881751705E-4 5.722049309044665E-4 0.0 0.7099275052115352 5.006793145414082E-4 76-77 8.225445881751705E-4 5.722049309044665E-4 0.0 0.7937555275890396 5.006793145414082E-4 78-79 8.225445881751705E-4 5.722049309044665E-4 0.0 0.8912807055000695 5.006793145414082E-4 80-81 8.225445881751705E-4 5.722049309044665E-4 0.0 1.0082250882536699 5.364421227229373E-4 82-83 8.225445881751705E-4 5.722049309044665E-4 0.0 1.1335200867176574 5.722049309044665E-4 84-85 8.225445881751705E-4 5.722049309044665E-4 0.0 1.2551851601512194 5.722049309044665E-4 86-87 8.225445881751705E-4 5.722049309044665E-4 0.0 1.3911553568573933 5.722049309044665E-4 88-89 8.225445881751705E-4 5.722049309044665E-4 0.0 1.547027556316588 5.722049309044665E-4 90-91 8.940702045382289E-4 5.722049309044665E-4 0.0 1.7258058344160523 5.722049309044665E-4 92-93 8.940702045382289E-4 6.079677390859956E-4 0.0 1.9208740716422033 5.722049309044665E-4 94-95 8.940702045382289E-4 6.079677390859956E-4 0.0 2.1165681580115305 5.722049309044665E-4 96-97 8.940702045382289E-4 6.079677390859956E-4 0.0 2.319200229168075 5.722049309044665E-4 98-99 9.834772249920519E-4 6.079677390859956E-4 0.0 2.5476709292357738 5.722049309044665E-4 100-101 0.0010371214372643456 6.079677390859956E-4 0.0 2.812583930840451 5.722049309044665E-4 102-103 0.0010728842454458746 6.437305472675247E-4 0.0 3.0982930054026876 5.900863349952311E-4 104-105 0.001233816882262756 6.437305472675247E-4 0.0 3.3855398807167294 6.437305472675248E-4 106-107 0.0013232239027165787 6.616119513582893E-4 0.0 3.671248955278966 6.79493355449054E-4 108-109 0.0013411053068073434 6.79493355449054E-4 0.0 3.9813482650210053 7.152561636305831E-4 110-111 0.0014126309231704015 6.79493355449054E-4 0.0 4.337796174166306 8.404259922659351E-4 112-113 0.0014662751354426952 6.79493355449054E-4 0.0 4.731473166628579 8.583073963566997E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7555 0.0 37.84873 1 ATCAACG 8595 0.0 32.50543 3 TCAACGC 8650 0.0 32.50535 4 CAACGCA 8850 0.0 31.770763 5 GTACATG 12865 0.0 31.163754 1 TACATGG 12635 0.0 30.504082 2 TATCAAC 9320 0.0 30.296421 2 AACGCAG 9035 0.0 30.104774 6 ACATGGG 12950 0.0 29.486088 3 GGTATCA 5215 0.0 28.786621 1 TAGGACG 850 0.0 25.930548 4 ACGCAGA 10535 0.0 25.419271 7 TACCTGG 2710 0.0 25.059044 2 TAGCCCT 2800 0.0 24.040823 4 CGCAGAG 10325 0.0 23.972345 8 GTGTAGC 2520 0.0 23.876207 1 CATGGGT 3400 0.0 23.47766 4 GTATAGG 820 0.0 23.247732 1 GTACCTG 3110 0.0 22.411451 1 GAGTACA 10350 0.0 22.389984 1 >>END_MODULE