FastQCFastQC Report
Thu 26 May 2016
SRR1364624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796201
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82400.2946855394158002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72060.25770679575609906No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47250.16897926865772525No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76000.047.106531
GTATCAA116300.043.024891
TATCAAC144300.034.53272
ATCAACG145200.034.29843
TCAACGC145350.034.0198254
CAACGCA147200.032.980295
AACGCAG152450.032.0312926
GTACATG133450.029.9518831
TACATGG131200.029.7674372
ACATGGG134400.027.979043
TAGGACG7250.027.905324
GTATCGC1505.262851E-627.7904016
ACGCAGA180450.026.9295487
CGCAGAG181050.026.8074118
TACCTGG27500.024.030442
GTACCTG29100.023.9504181
GAGTACT112200.023.5925412-13
GCAGAGT200550.023.370679
GTACTTT116250.022.82183314-15
AGTACTT117700.022.36359412-13
Produced by FastQC (version 0.11.5)